International Journal of Clinical Biochemistry and Research最新文献

{"title":"About the many faces of autism spectrum disorder: from psychoanalysis to biological markers. Could the methionine sulfoxide reductase enzymes play a role?","authors":"C. Achilli, A. Ciana, G. Minetti","doi":"10.18231/j.ijcbr.2022.053","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.053","url":null,"abstract":"Autism spectrum disorder is a dramatic condition for the affected children, their families and the entire society. Genetic and environmental factors are involved in its pathogenesis and oxidative stress appears to be the main trigger. Methionine sulfoxide reductase enzymes are physiologically involved in the contrast to oxidative stress and disorders in their expression/activity could result in several diseases. In the fight against autism spectrum disorder, this neglected biochemical feature should be taken into account.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87695631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of D-dimer with estimated glomerular filtration rate in chronic kidney disease at different stages in Indian population","authors":"K. Ramprasad, M. Siddappa","doi":"10.18231/j.ijcbr.2022.061","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.061","url":null,"abstract":": Hyper-coagulability followed by cardiovascular disease is the most common cause of mortality in CKD (chronic kidney disease). Diagnostic usefulness of hypercoagulability marker at various stages of CKD needs to be assessed. : An observational cross-sectional study.: Dept of Biochemistry, Institute of Nephrourology, Bangalore, India from January 2022 to March 2022.Study population consists of CKD patients attending Nephrology outpatient for follow up. They were categorized into 5 stages using eGFR values. Laboratory test including D-Dimer was assayed in all the participants using Abbott Architect ci4100 analyzer. In our study, a peaked and statistically significant (P value = <0.0001) D-Dimer values were observed in stage 4&5 (5.4 ±2.5) in comparison with stage 1, 2 & 3 (0.9±0.2). D- Dimer had strong negative correlationR= - 0.79 with eGFR in stage 5 CKD. D-Dimer assay should be considered as a part of routine investigations in CKD patients especially in developing countries like India, where most of the patients reach hospital only at the later stages of the disease, in order to achieve a better follow-up and management of the disease.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72875535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of MMP-9 with atherogenic index and electrocardiogram as a predictor of cardiovascular risk factors in hypertensive patients","authors":"G. Gupta, R. Jain, B. Das, S. Saxena","doi":"10.18231/j.ijcbr.2022.056","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.056","url":null,"abstract":"Hypertension is the strongest or one of the strongest risk factors for almost all different cardiovascular diseases acquired during life, including coronary disease, left ventricular hypertrophy, and valvular heart diseases. So, the aim of this study was to evaluate the plasma concentration of MMP-9 and AIP in mild to moderate hypertensive patients. : A total of 162 patients with hypertensive case were recruited and compared with the 100 normotensive controls from the Rohilkhand Medical College and Hospital Bareilly. Parameters include waist circumference (WC), Fasting Blood Sugar (FBS), Lipid profile with Atherogenic Index, and Matrix metalloproteinase-2 were estimated in the plasma of all the test groups. Electrocardiography (ECG) was also performed as a cardiovascular tool.There was a significantly increased concentration of WC, FBS, and Lipid parameters except for HDL which is slightly lower in the case group when compared to the control group. The concentration of MMP-9 was also increased, which is positively significant. The mean value of the P-R interval, QRS complex, and QT interval was also increased in hypertensive as compared to the controls.Atherogenic index and MMP-9 are significantly higher in hypertensive patients. So, it could be a biomarker of cardiovascular disease.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76721857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Dyslipidemia with HbA1c levels in Diabetes Mellitus","authors":"R. Malathi, A. Fatima, Mohd Iqbal Ahmed","doi":"10.18231/j.ijcbr.2022.060","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.060","url":null,"abstract":"Diabetes is a metabolic disease which has hyperglycaemia resulting from defects in insulin secretion or action. HbA1C is one of the most reliable test used for estimation of glycaemic index. Diabetes is often accompanied by undiagnosed dyslipidaemia, characterized by increased triglyceride level, decreased high-density lipoprotein levels and increase in low-density lipoprotein (LDL) particles. Diabetes is a significant risk factor for atherosclerotic cardiovascular disease (ASCVD). The purpose of this study was to correlate lipid parameters with different levels of HbA1c to assess dyslipidaemia with diabetic status. An observational study was conducted in biochemistry laboratory at Gandhi Hospital, Telangana.After informed consent, 5ml of venous blood sample was collected in plain tube and EDTA tube from diabetic and apparently non diabetic patients. Estimation of serum triglycerides was done by glycerol phosphate oxidase peroxidase, serum cholesterol by cholesterol oxidase method, serum HDL by direct clearance method in Beckmann Coulter AU 500 and HbA1c was estimated by high performance liquid chromatography in Bio-Rad D10.A total of 90 samples were divided into 3 groups according to their HbA1c results. Group 1 with HbA1c less than 5.6, Group 2 with HbA1c 5.6-7 and Group 3 with HbA1c more than 7.The present study showed no variation in the lipid profile in Group1 with HbA1c < 5.6. In Group 2 with HbA1c between 5.6-7, there was significant increase in LDL cholesterol. In Group 3 with HbA1c >7 there was marked increase in LDL, serum triglyceride. Hence we can conclude that dyslipidemia is increased in poor glycemic control and evaluation of HbA1c may also be used as a predictor of dyslipidaemia in diabetics.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72757591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in breast cancer patients of Bihar: Case-control study","authors":"Tanu Verma, R. Lal, Manish Kumar, Uday Kumar, S. Shahi","doi":"10.18231/j.ijcbr.2022.059","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.059","url":null,"abstract":"Breast cancer (BC) is the most frequent cancer in women globally and the main cause of cancer-related mortality. MTHFR C677T is a functional polymorphism in the genes encoding a one-carbon metabolism enzyme that affect folate metabolism and may be associated with breast cancer susceptibility. We have conducted a case-control study taking 100 cases of diagnosed breast cancer and 100 healthy control. PCR-RFLP technique was used to detect the genotype of C677T polymorphism in the study population.About 35% of cases had normal genotype(C677C), 49% had the genotype C677T & rest 16% had the genotype T677T for MTHFR. Among control the frequency of C677C genotype was 55% C677T was 40% & T677T was 5%. C677T & T677T MTHFR genotype were more common in case as compared to control [χ2 (2, N = 200) = 11.12, p = 0.004]. T allele frequency was significantly higher in case (40%) as compared to control (5%) [χ2 (1, N = 400) = 10.91, p = 0.001]. Cases with MTHFR 677TT genotype had a significantly higher risk of BC than 677CC individual [OR=1.64 (1.03 to 2.10)] and increasing T-allele was significant in BC [OR=1.07 (0.71 to 1.33)] (P for trend = 0.04).C677T of MTHFR gene has a strong correlation with breast cancer in the studied population and can be used as a prognostic marker for the development of breast cancer.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79568201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of ‘Role play’ in teaching ‘Progression of Learning’ in all the three domains [cognitive, psychomotor and affective] for medical teachers","authors":"P. Agrawal","doi":"10.18231/j.ijcbr.2022.067","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.067","url":null,"abstract":"Role play is an important and interesting educational tool and it provides ample opportunity for learner to learn at both cognitive and affective domain simultaneously. Role play is favoured by both learners and instructors at the same time, as this activity breaks the monotony, brings variation and make the learner active and involved. Each domain of learning has multiple levels of learning that progresses from more basic surface level learning to more complex deeper level learning. Author wish to share her personal experience of implementing role-play as a novel approach to teach three learning domains and the progression of learning. Participants were faculty who were attending revised basic course workshop. All the participants were divided into three groups representing one domain of learning. Group one represented cognitive [knowledge] domain. Group two represented psychomotor [skill] domain. Group three represented affective or attitudinal domain. They were assigned task related to their domain and wee asked to express progression of learning of their domain. All the participants found activity very useful and enjoyed learning the progression of learning in an enjoyable manner.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78861143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular differentiation of dengue virus serotypes using RT-PCR and estimation of their effect on liver function","authors":"Dr. Abhinav Manish, P. Pandey, N. Sharma","doi":"10.18231/j.ijcbr.2022.065","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.065","url":null,"abstract":"Dengue is one of the common and leading cause of mortality and morbidity in tropical countries specially in India. Its hepatotoxic effects have been demonstrated by the researchers over a long time. Dengue virus is a RNA virus, and known to have four serotypes and recently a fifth variant is also demonstrated in India. The effect of the different serotypes is seldomely investigated by the researchers. So we try to estimate the effect on Liver due to infection via different serotypes of Dengue virus. After taking the ethical clearance, from the ethical committee, A hospital based prospective study was conducted from August 2017 to November 2018, on patients attending OPD and IPD, of Medicine Department at Shri Mahant Indiresh Hospital Dehradun. 60 Dengue Positive cases were selected. RNA gets extracted using Reverse Transcriptase PCR, Conventional PCR and Real Time PCR techniques and further visualized using Agarose gel electrophoresis techniques at the CMRL (Central Molecular Research Laboratory), and liver Enzymes SGPT, SGOT, ALP, GGT were analyzed at Central Clinical Laboratory of SMI Hospital using the vitros 5600 fully autoanlyzer of the Orthoclinical diagnostics. Results were analyzed using suitable statistical tools.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"180 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80682569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Lab request forms: How do our clinicians communicate with Laboratory?","authors":"D. Trivedi","doi":"10.18231/j.ijcbr.2022.066","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.066","url":null,"abstract":"Central clinical laboratory is an important division in the health care setup. Laboratory results help clinical decisions, follow up care and ensure patient safety. Laboratory request Form [LRF] is an important medium between the patient, a treating clinician and Laboratory. A meticulously filled LRF is important for patient care. Providing accurate and complete information in LRF is the doctor's responsibility. Erroneous LRF will have an impact on the quality of laboratory results. Present study evaluates the degree of completeness and correctness of quality indicators on laboratory request forms [LRF] to examine preanalytical standards of laboratory services.This study is a single center, prospective, cross sectional, descriptive type conducted at a 650 bed teaching hospital from Gujarat. In the span of a six months study period, 3735 [20% of total] LRFs were selected by simple random sampling method from the total LRF received at OPD blood collection center. They were analyzed for patient, clinician and sample identifier quality indicators along with completeness and correctness. Qualitative information was converted to quantitative by using two point scale, 0 score for incomplete information and 1 score for complete information.Among patient identifier quality indicators name, age, gender and location were filled in more than 75% forms whereas, very poorly filled 2% provisional diagnosis and 42% MRD number. Clinician identifier quality indicator was attended to in less than 50% forms. Time and date of request were absent on 100% forms. Sample identifier quality indicator shows 97% forms with the nature of the sample and 92% having investigation requests. Test requests on one third forms were invalid and inappropriate. 38% forms were incomplete and inappropriate whereas 46% forms had error in filling one or other data indicators.Appropriately filled LRF communicates well with the Central clinical laboratory. It will help in providing quality reports in time and benefit clinicians to manage quality care for patients. Hand written, poorly legible, inappropriately abbreviated, erroneous LRF are misleading and may compromise laboratory service and patient safety. Training and change in attitude towards LRF writing is required to maintain the standard of the health care system.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80739213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue fever: A global threat to human beings","authors":"A. K. Shah, Alisha Wadhwa, Bhawna Tyagi, Mukul Mudgal","doi":"10.18231/j.ijcbr.2022.054","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.054","url":null,"abstract":"The RNA virus of the Flaviviridae family, which causes the acute viral sickness known as dengue, is transmitted by Aedes mosquitoes. Asymptomatic fever to terrifying consequences including hemorrhagic fever and shock may be the presenting symptoms. The most typical symptoms include acute-onset high fever, muscle and joint pain, myalgia, cutaneous rash, hemorrhagic episodes, and circulatory shock. Even though oral symptoms are rarely the main presenting symptom of dengue infection, this can happen in some situations. To reduce mortality, an early and precise diagnosis is essential. Despite the fact that dengue virus infections are normally self-limiting, dengue infection has become a public health concern in tropical and subtropical regions. Infections with the dengue virus, their various clinical symptoms, diagnosis, differential diagnosis, and prevention are the important concern of this paper.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83883377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of glutathione s-transferase pi (GSTP1) gene polymorphism in unexplained infertile women","authors":"S. Vanitha, V. Job, Aleyamma T K, Marimuthu S, L. Jeyaseelan","doi":"10.18231/j.ijcbr.2022.051","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.051","url":null,"abstract":"15–20 million people in India are affected by infertility.Among them, 34% of the couples are shown to have unexplained infertility (UEI). Both genetic and environmental factors influence UEI. Data from various studies show that oxidative stress plays an important role in unexplained infertility. The genes of the phase II detoxification enzyme, Glutathione s-transferase family are upregulated in humans as a defense mechanism opposing the adverse effects of oxidative stress and play important role during pregnancy. Since all investigations for infertility work-up are normal evaluating the causes for UEI will have impact on the treatment protocol. In this study the association between GSTP1 variations and unexplained infertility are discussed. To compare the association of GSTP1 polymorphism in women with unexplained infertility and a control group.: This is a case control study with 70 normal ovulatory women who conceived within 12 months of contraceptive free intercourse, and with no history of miscarriage were recruited in the control group and 70 women with unexplained infertility were recruited as study group. All participants included in the study were between 28 and 38 years of age. The association of GSTP1 polymorphism were studied using real-time PCR with the Light Cycler instrument (Roche Applied Science).: GSTP1 variant allele frequencies were 0.39 and 0.41for control and cases respectively. The results of this study indicate GSTP1 genetic polymorphisms did not show a significant association with unexplained infertile group.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84987322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}