International Journal of Clinical Biochemistry and Research最新文献

{"title":"Role of nanoparticles in maintaining food safety and tackling malnutrition","authors":"S. Kushwaha, H. Pant","doi":"10.18231/j.ijcbr.2023.004","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.004","url":null,"abstract":"The food varieties which complete what seems like a forever cycle from development to bundling through nanotechnology are known as Nano food, for instance, we are devouring some braced food that has nanoparticles like iron, zinc, omega 3, and coenzymes. Numerous food sources normally having safe nanoparticles, get processed in the body at the nanoscale. Nano food sources have high dietary benefits and less bad cholesterol. They are the cutting-edge food that may battle against Malnutrition. There are more than 500 nano foods accessible in the market which can be sorted as Nanoemulsion, Nano container, and bundled food. Nanoparticles are additionally utilized as a protected palatable covering that monitors our food against parasite, dampness, and gas trade in this way expanding the self-existence of food. Nanoparticles have an extraordinary job in food bundling.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"476 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79936125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of progressive muscle relaxation technique on physical parameters and anxiety among patients with myocardial infarction","authors":"Sudhir Kumar Khuntia, Reena Thakur","doi":"10.18231/j.ijcbr.2023.006","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.006","url":null,"abstract":"The main aim of the study is to assess the effect of progressive muscle relaxation therapy on physical parameter and anxiety among patient with myocardial infarction admitted at selected hospitals of Durg (C.G). The study was conducted at B.M Shah Hopsital and K. Gurunath Cardiac Hospital for patient with myocardial infacrtion.Quasi experimental pretest posttest control group design was adopted for this study. Convenient sampling technique was used to select the study participants. The patient with myocardial infarction was assigned to experimental group (n=23) and the other to the control group (n=23). Progressive muscle relaxation therapy was given for 20 minutes once daily in the morning between 6-8AM for 7 days. Physical parameter and anxiety measurements were taken from the first day and 7 day and was documented. The study showed a significant reduction of physical parameters mean blood pressure from 5.75 to 5.5 mm of Hg, for pulse 5.5 to 5, for respiration 7 to 5.7, and SPO2 7 to 5.8. calculated ‘t’ value was greater than the table value at 0.001 level of significance. Level of anxiety before and after progressive muscle relaxation among the experimental group was 50and 44.5. respectively. Standard deviation was 7.0, 6.6 and the calculated ‘t’ value was 3.4. While comparing with table value, it showed that the calculated ‘t’ value was greater than the table value at 0.001 level of significance.Hence it is concluded that progressive muscle relaxation therapy is an effective measure to maintain the level of physical parameters and reduce the level of anxiety among patient with myocardial infarction at selected hospitals of Durg (C.G).","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84764359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of allergen specific immunoglobulin e testing in Indian population: A retrospective study","authors":"F. Almeida, J. Surana, A. Christy, R. Jatale, Shibani Ramchandani","doi":"10.18231/j.ijcbr.2023.002","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.002","url":null,"abstract":"Prevalence of allergies has increased dramatically in recent decade and is now considered major chronic disease at the global level. Measurement of total and allergen specific IgE (Immunoglobulin E) is often requested to assess possible allergy. The main objective of our study was to understand the relevance of allergen specific IgE testing in Indian population. This retrospective study was conducted at a Global Reference Laboratory in Mumbai from May 2020 to May 2022. Total available data of 65431 patients was included in the study. The data was sub grouped as per age group, gender, and positivity for specific allergens. Of the total 65431 patients, 34601 (52.88%) were Females and 30830 (47.12%) were Males. Among those tested for specific IgE allergen, positivity was found to be 30%. The dust allergy was the most prevalent (62.18%) followed by Insect allergy (52.69%) and food allergy (51.78%). Out of the 18377 patients tested for Specific allergens, 7340 (39.94%) had at least one allergen positive. The availability of multiple and allergen specific panels has proved to be a major tool for the detection and diagnosis of multiple allergies. Detection of allergy is necessary to avoid wrong diagnosis and mismanagement of disease. Combination of total IgE with specific IgE can be used as evidence of allergy along with clinical findings. It is recommended to proceed with clinically appropriate allergen specific IgE tests, regardless of total IgE concentration in patients with a history of an acute allergic reaction.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82788622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four hundred IU vs One thousand IU of vitamin D supplementation in first episode of nephrotic syndrome","authors":"S. Lekhwani, N. D. Vaswani, Sudeep Kumar, P. Kamboj","doi":"10.18231/j.ijcbr.2022.062","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.062","url":null,"abstract":"Use of steroids in nephrotic children may lead to changes in bone mineral density and osteoporosis eventually affecting growth on a long term basis. We compared the proportionate changes in bone mineral content (BMC) and density(BMD), Vitamin D levels, Serum Calcium, phosphate and alkaline phosphatase levels in nephrotic children with the aim of giving high Vs low vitamin D doses (1000 IU Vs 400 IU) to two groups; group 1 (n=20) vs group 2 (n=20) respectively. The median BMC in group 1 increased from 11.53±3.48 g to 11.61±3.54 g after 1000 IU Vitamin D supplement and was statistically significant. However group 2 showed insignificant increases in BMC from 11.24±2.71 g to 11.25±2.67 g following 400IU Vitamin D. The change in BMD observed in group 1 from a mean of 0.426 to 0.429g/cm2 whereas in group 2 with 400 IU of vitamin D it didn’t show any significant change. The median vitamin D increased significantly in both groups; from 16.62±7.20 ng/ml to 27.45±6.47 ng/ml in group 1 while in group 2 from 18.72±8.07 ng/ml to 26.18±7.61 ng/ml which was statistically significant. The serum calcium levels normalized irrespective of 1000 IU or 400 IU of vitamin D supplementation. Changes in serum phosphate levels (decline from initial) were statistically significant however the changes in serum ALP were insignificant. We concluded that children supplemented with 1000 IU /day of vitamin D had better osteoprotection as compared to the other group.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77648733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic role of different biochemical parameters in pleural effusion","authors":"Jaspreet Singh, Khushdeep Singh, Rincal Saini, Umesh Kumar, Shiv K. Sharma, H. Singh","doi":"10.18231/j.ijcbr.2022.057","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.057","url":null,"abstract":"A pleural effusion, an excessive accumulation of fluid in the pleural space, indicates an imbalance between pleural fluid formation and removal. For diagnosing and treatment plan, pleural effusions have to be classified into transudate and exudate. The aim of present study was to analyze various biochemical parameters (LDH, pH, Glucose, Triglycerides, Cholesterol, Creatinine, Amylase and ADA) in pleural fluid and to correlate these Biochemical parameters with diagnosis of the patients. The study was a hospital based descriptive study. The study was conducted over a period of one year on 100 samples. Total 100 samples were enrolled in the study. Both serum and pleural fluid samples were collected and quantitatively analyzed using semi-automated analyzer. The results of the present study shows thatThe Mean±SD of concentration of glucose in pleural fluid was 65.69±14.13 mg/dl, total protein 3.53±1.53g/dl, albumin 1.64±0.85g/dl, cholesterol 58.9±13.05mg/dl, triglycerides 68.7±10.47mg/dl, creatinine 1.73±0.96mg/dl, amylase 49.56±17.78IU/L, LDH 299.82±65.46 U/L and ADA was 49.56±17.78U/L.It was concluded that Biochemical parameters play important role in diagnosing Pleural effusions. These markers when used collectively their diagnostic efficacy is greatly increased. The SEAG is superior to Light's criteria in identifying the transudative effusions. It is also observed that Light's criteria identified exudative effusions better than SEAG.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84321491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to avoid analytical errors in blood gas analysis","authors":"P. K. Nigam","doi":"10.18231/j.ijcbr.2022.068","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.068","url":null,"abstract":"","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83328312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum protein electrophoresis in patients with renal disorders","authors":"Shiv K. Sharma, D. Soin, H. Singh, Khushdeep Singh","doi":"10.18231/j.ijcbr.2022.058","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.058","url":null,"abstract":"One of the cardinal findings of the renal disease is proteinuria, which appears in the early phase of kidney disease and is very important in diagnosis, prognosis and decision-making in treatment process and results of treatment. Besides this abnormal protein fractions are commonly observed in renal disorder. Serum will be collected for protein electrophoresis and Quantization of each fraction was done. So the present study is designed to find the role of serum protein electrophoresis in renal disorder patients.The aim of the present study was to study protein electrophoresis pattern in various renal disorders.The present study was a hospital-based observational study. The study was conducted over a period of 1 year on 50 patients with renal disorder of either sex visiting the OPD/IPD of Medicine department of Guru Gobind Singh Medical College and Hospital Faridkot. SDS Page electrophoresis was done to evaluate serum proteins in the patients with renal disorders.The results of the study showed the comparison of protein content in patients of NS and ESRD at p<0 Albumin(t=3.86), α1 Globulin(t=6.18), t=-2.76)>0.05). The Comparison of protein content in patients of NS and CKD at p<0 Albumin(t=5.42), α1 Globulin(t=3.38)>0.05).The present study concluded that serum protein fractions are altered in various renal disorders. Protein electrophoresis should be used in addition to other renal markers for better monitoring of the disease progression.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74051734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti oxidative activities of aqueous and ethanolic extracts of stem barks from trichilia emetica in albinos rats","authors":"Dere Kwadjo Anicet Luc, D. Prisca, Manhan Kahissié, Y. Félix, T. G. Georges","doi":"10.18231/j.ijcbr.2022.064","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.064","url":null,"abstract":": In chronic diseases in general and in diabetes mellitus and hypertension in particular, there is an important imbalance between antioxidant defenses and the production of free radicals due to reactive oxygen species, thus leading to an increase in oxidant stress markers. Therefore, this study was designed to evaluate the antioxydative and protective effects of aqueous and ethanolic extracts of trichilia emetica stem bark in albinos rats with alloxan-induced diabetes or adrenaline-induced hypertensive.: Two different batches of albinos rats were used distinctly and including a control one of 3 rats for each batch so a test group of 21 rats. Diabetes was induced by injecting Alloxane® intraperitoneally for 7 days. Hypertension was induced by injecting Adrenaline® intraperitoneally for eight days and we used visitech BP 2000 tools to measure arterial pressure. The aqueous and ethanolic extracts of Trichilia emetica were prepared and the obtained mixture was homogenized using a magnetic stirrer for 24 hours. Rats received the above-mentioned extracts orally at doses of 100 and 200 mg/kg bw for 6 days.: Aorta and heart activities of catalase were significantly increased. This same trend was observed with superoxide dismutase activity together with increasing AChE catalytic activity in vessels. Treatment of rats for seven days with extracts of Trichilia emetic and nifedipineproduced a significant decrease of antioxidative markers activities of catalase and SOD for aqueous and ethanolic extracts as well. This action was slightly above nifedipineone administrated at 10 and 20 mg/kg bw. For rats treated with Trichilia emetica or with diastabolwe registered a significant decrease of MDA and GSH concentration.Our results showed that the extracts have the potential to reduce the rate of reactive oxygen species and free radicals in rats with alloxane-induced diabetes or adrenaline-induced hypertensive. However, further large studies are needed to investigate the specific action and particularity of Trichilia emetica that could justify these activities.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84605166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of biosensors - A short review","authors":"Dr. Abhinav Manish","doi":"10.18231/j.ijcbr.2022.055","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.055","url":null,"abstract":"Enzyme-based, tissue-based, immunosensors, DNA biosensors, thermal and piezoelectric biosensors are explored here, revealing applications in medical fields to distinguish between natural and man-made substances. Some industries have implemented the use of biosensors, such as the food industry. Biosensors are used in metabolic engineering to enable in vivo monitoring of cell metabolism, in order to obtain accurate glucose concentrations, in fermentation industries and in saccharification processes to control its quality and safety. Biosensors and their role in medicine, including early detection of human interleukin 10, which causes heart disease, and rapid detection of human papillomavirus (HPV). It's an important aspect. Fluorescent biosensors play an important role in drug discovery and cancer diagnosis. Biosensing applications are widely used to find missing links in metabolic processes. Basically, biosensors serve as inexpensive and highly efficient devices for these purposes in addition to other routine applications.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89439106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II type I receptor gene polymorphism in essential hypertensive and normotensive subjects","authors":"H. Sharma, Sapna Patel, Shreyansh Sharma, Drashti Rao, Jagruti Dholakia, Hina Baraiya","doi":"10.18231/j.ijcbr.2022.063","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2022.063","url":null,"abstract":"Hypertension is a polygenic disorder that involves a complex interplay of genetic and environmental factors. Genes that influence the renin–angiotensin–aldosterone system (RAAS) appear to be particularly important among the many potential candidate components of EHT. The RAAS system plays an important role in BP regulation by maintaining vascular tone and renal hemodynamics. Most of these are mediated via the activation of the angiotensin II type 1 receptor (AT1R). Untreated hypertension increases the risk of developing cardiovascular diseases such as stroke, myocardial infarction, ischemic heart disease, and, target organ damage. A polymorphism in the 3’ untranslated region of the AT1R gene has been described with either an adenine (A) or a cytosine (C) base (A/C transversion) at the 1166 position that is associated with increased blood pressure. A total of 170 hypertensive patients and 170 normal subjects were included in the present study. Genomic DNA was isolated from peripheral blood leukocytes, amplified with PCR, and separated with gel electrophoresis to determine the AT1R genotype. On electrophoresis, the genotypes A/A-359 bp, A/C-220+139 bp, and C/C-220, 139, and 359 bp were identified. In hypertensive patients, the CC genotype was present in 21.18 percent (n = 36) as compared to 11.76 percent (n = 20) in non-hypertensive patients. In this study AT1R CC homozygote was significantly associated with hypertension (p = 0.019). The odds ratio related to the association of the prevalence of the CC genotype with hypertension was 2.014 (95% CI: 1.112–3.650).: From the present study, it is concluded that AT1R gene polymorphism is associated with hypertension, and with the CC genotype, the risk of hypertension increases in comparison to AC and AA. A allele is not significantly associated with hypertension, but the recessive and co-dominant modes of inheritance of allele C (CC) and its co-dominant mode (AC) are a genetic predisposition to hypertension.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81137083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}