International journal of biochemistry and molecular biology最新文献

{"title":"Aleppo galls alleviate paracetamol-induced hepatotoxicity and tissue damage: an experimental study.","authors":"Ahmed Alamir Mahmoud Abdallah,&nbsp;Emad A Albadawi,&nbsp;Moutasem Salih Aboonq,&nbsp;Maha K Desouky,&nbsp;Ahmed Rh Ahmed,&nbsp;Rawan Bafail,&nbsp;Osama B Abdel-Halim,&nbsp;Mehrevan M AbdElmoniem,&nbsp;Ahmed J Aldhafiri,&nbsp;Ali Alalawi,&nbsp;Faten M Omran,&nbsp;Wafaa A Abdellah,&nbsp;Azza Ma Abouelella,&nbsp;Abdelaziz Aa El-Sayed,&nbsp;Amal Yaseen Zaman,&nbsp;Nawal Almohammadi,&nbsp;Sultan S Al Thagfan,&nbsp;Ibrahim M Abdel-Rahman,&nbsp;Abdullah Mahfouz Alsharif,&nbsp;Mariam Eid Alanazi,&nbsp;Salah Mohamed El Sayed,&nbsp;Hussam H Baghdadi,&nbsp;Hytham Mahmoud Abdel-Latif","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Acute paracetamol toxicity is a common and potentially life-threatening emergency causing liver failure that may necessitate liver transplantation. Unfortunately, current therapies are still defective.</p><p><strong>Objectives: </strong>To investigate the protective effects exerted by Aleppo galls (Quercus infectoria Olivier) extract against acute paracetamol toxicity in mice.</p><p><strong>Methodology: </strong>Eighteen mice were divided into three experimental groups, each included six mice in each group. The groups included: negative control group, paracetamol toxicity group that received an acute toxic intraperitoneal dose of paracetamol (250 mg/kg) for four consecutive days, and treatment group (received 250 mg/kg paracetamol followed few hours later by Aleppo galls extract for the same duration). Animals were anaesthetized using ether anaesthesia. Animals were sacrificed by decapitation and blood samples were drawn. Paracetamol toxicity effects versus Aleppo galls protection were evaluated on liver function tests, liver histology, serum cholesterol and serum triglycerides.</p><p><strong>Results: </strong>Acute paracetamol toxicity caused significantly elevated serum transaminases (ALT and AST), decreased serum albumin, and increased serum cholesterol and triglycerides. Aleppo galls extract exerted significant protective effects and restored near normal serum levels of the previously-mentioned parameters. Upon histopathological evaluation, mice in the control group showed normal hepatic architecture with preserved hepatic cords and sinuses. Acute paracetamol toxicity induced peripheral zonal degeneration with focal necrosis of the hepatic tissue. The hepatocytes showed cytoplasmic vacuolation with indistinct cell borders. Central hepatic venules were congested. Administration of Aleppo galls extract reduced the tissue damaging effects induced by paracetamol toxicity with only minimal residual degenerative changes that were observed with absent necrosis.</p><p><strong>Conclusion: </strong><i>Quercus infectoria Olivier</i> (Aleppo galls) is a promising source of phytochemicals and future therapeutics.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"14 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018004/pdf/ijbmb0014-0001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9514478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The variation of the androgen receptor single nucleotide polymorphism (rs6152) gene in different stages of endometriosis disease in Iraqi women","authors":"Mohammed Abdul-Jalil Z Alkaabi, Hedef Dhafr El-Yassin","doi":"10.33545/26646501.2023.v5.i2a.50","DOIUrl":"https://doi.org/10.33545/26646501.2023.v5.i2a.50","url":null,"abstract":"Gene (AR) is a nuclear receptor belonging to a family of super-nuclear futures. This study aims to screen the diagnostic and clinical of the (AR) gene single nucleotide polymorphism (rs6152) at various stages of endometriosis. Methods: 120 serums of Iraqi women suffering from endometriosis were taken and it was distinguished into (I, II, III, and IV) stages according to the “American Society of Reproductive Medicine” (ASRM) guidelines. Iraqi healthy women numbering 50 were taken as a control group. The statistical software (SPSS. 2017) Version (25.0) were used to analyze the data. Results: The (AR) gene single nucleotide polymorphism (rs6152) has three genotypes which are (AA, GA, and GG), 72% of the group in the control carry the (GA) genotype copy of the (AR) gene. The wild-type (GG) genotype was found in (85) patient cohort with a genotype frequency of (70.8%), while the mutant genotype (AA) was detected in 17 (14.2%), and (GA) was noticed in 18 patients with a genotype frequency (15.0%). Conclusion: The augmentation of frequency of the (GG) genotype of this SNP in different stages of endometriosis can be considered promising signs to identify and diagnose patients with endometriosis. Abbreviations: AR = androgen receptor, kDa = kilodaltons, DNA = Deoxyribonucleic acid, SNP= Single nucleotide polymorphism, bp = base pair.","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135008453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality control of Thuja occidentalis sesame oil by UV- VIS Spectrophotometer","authors":"Vaghasiya Dhruvi Keshubhai, Pramanick Monimala, Makwana Kosmika, Singh Suraj, B. Panda, Chakraborthy Gs","doi":"10.33545/26646501.2023.v5.i2a.46","DOIUrl":"https://doi.org/10.33545/26646501.2023.v5.i2a.46","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"231 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79695863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, antibacterial, antifungal activities and gas chromatographic fingerprint of fractions from the root bark of <i>Afzelia africana</i>.","authors":"Bright Yaw Vigbedor, Clement Osei Akoto, Ralph Kwakye, Jonathan Osei-Owusu, David Neglo, Pius Kwashie","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong><i>Afzelia africana</i> is a tropical plant with numerous ethno-medicinal benefits. The plant has been used for the treatment of pain, hernia, fever, malaria, inflammation and microbial infections.</p><p><strong>Objectives: </strong>To perform bioassay-guided fractionation, antioxidant and antimicrobial activities of the bark of <i>Afzelia africana</i>.</p><p><strong>Methods: </strong>Column chromatography fractionation, antioxidant activity (% (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl picrylhydrazyl (DPPH) scavenging activity))), antimicrobial activity (microbroth dilution: Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio), and synergistic activities (Checkerboard assay: Fraction Inhibitory Concentration Index (FICI)).</p><p><strong>Results: </strong>Bioassay-guided fractionation of <i>A. africana</i> produced four fractions that displayed promising free radical scavenging activities in the ABTS (54-93)% and the DPPH (35-76)% assays in the ranking order of F1(93-54)>F4(81-58)>F2(74-58)>F3(72-55) and F3(77-42)>F1(64-46)>F4(55-44)>F2(47-35) respectively at a concentration range of 1.0-0.01 mg/mL. The fraction F1 (MBC: 2.5-5.0 mg/mL) and F4 (MBC: 1.25-10.0 mg/mL) exhibited broad spectrum of superior bactericidal effects than F2 (MBC≥100.0 mg/mL) and F3 (MBC: 12.5-100.0 mg/mL) against <i>Staphylococcus mutans, Staphylococcus aureus, Escherichia coli</i>, fluconazole-resistant <i>Candida albicans</i>, methicillin-resistant <i>S. aureus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi</i>, and <i>Candida albicans</i> (standard strain). The two most active fractions (F1 and F4) reported synergistic effects (FICI≤0.5) against <i>S. typhi</i> whilst the F4 reported additional synergism against <i>E. coli, K. pneumonia</i>, and <i>S. typhi</i> when combined with ciprofloxacin. Furthermore, the two fractions reported synergistic effects against <i>Escherichia coli, Klebsiella pneumonia, Salmonella typhi</i>, and <i>Pseudomonas aeruginosa</i> when combined with tetracycline whilst F1 reported antifungal synergism against fluconazole resistant <i>Candida albicans</i> when combined with fluconazole and ketoconazole.</p><p><strong>Conclusion: </strong>The study has confirmed the antioxidant, antimicrobial and synergistic uses of <i>A. africana</i> for the treatment of both infectious and non-infectious disease.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 6","pages":"60-76"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884338/pdf/ijbmb0013-0060.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10593447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of clinical outcomes, laboratory and imaging data of patients with solid tumor infected with COVID-19 infection.","authors":"Somayeh Sadeghi, Mohammadsaleh Peikar, Erfan Sadeghi, Ali Darakhshandeh, Safie Ghafel, Marzieh Aalinezhad, Alireza Sadeghi, Mehran Sharifi, Elahe Nasri","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 is associated with higher mortality rates in patients with cancer. In this study, we aimed to evaluate the clinical outcomes, and laboratory and imaging data of patients with solid tumor infected with COVID-19 infection.</p><p><strong>Methods: </strong>This is a cross-sectional retrospective study performed in 2020-2022 on 85 patients with a previous diagnosis of solid tumors infected with COVID-19. We included all patients with tumors of solid organs that were diagnosed with COVID-19 infection and required hospitalization those patients previously hospitalized for treatments and were infected with COVID-19 during hospitalization. Demographic data of patients were collected using a checklist. We collected data regarding clinical outcome (discharge, hospitalization or death), duration of hospitalization, requiring ICU admission, duration of hospitalization divided by received drugs and type of tumor and mean survival time. Furthermore, we collected laboratory data from all patients. The radiologic characteristics of patients were also extracted from their data.</p><p><strong>Results: </strong>Breast cancer was the most common solid tumor (34.9%), followed by lung cancer (19.3%). The mortality rate was 24.1% (20 patients). The highest mortality rate in this study was for metastatic intestinal cancer to the lung (100%, one patient), followed by metastatic prostatic cancer to lung (50%, three patients). The highest hospitalization duration was for patients with glioblastoma multiform (GBM) (30 days). The mean survival time among patients with mortality was 19.15±1.80 days. The mean CT severity score of all patients was 27.53±22.90. Patient's most common radiologic sign was air space consolidation (89.1%). The highest CT severity score was found in patients with stomach cancer (46.67±5.77).</p><p><strong>Conclusion: </strong>The mortality rate in this study was 24.1%. Based on the results of our study and previous research, special care should be provided to patients with solid tumors during the COVID-19 pandemic and in infected cases.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 6","pages":"77-86"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884339/pdf/ijbmb0013-0077.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10593446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent monkeypox and COVID-19: role of interleukin-1 receptor antagonist-like protein mechanism.","authors":"Rujittika Mungmunpuntipantip,&nbsp;Viroj Wiwanitkit","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>In 2022, monkeypox becomes a new global public health threat. Monkeypox has been present in Africa for a very long time. The COVID-19 epidemic has been affecting this region for some years. The outbreak state persists. The coexistence of the two diseases is an intriguing discovery. There is no documentation of the co-occurrence.</p><p><strong>Methods: </strong>The possibility of coexisting of the two lethal illnesses, monkeypox and COVID-19, is an intriguing scientific subject. Using bioinformatic pathophysiological pathway analysis, the authors investigated the pathophysiological pathways of COVID-19 and monkeypox.</p><p><strong>Results: </strong>The most common pathway was identified. An interleukin-1 receptor antagonist-like protein has been identified as a common pathway between the pathogenic processes of monkeypox and COVID-19.</p><p><strong>Conclusion: </strong>The findings could explain why severe COVID-19 and monkeypox do not coexist.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 5","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678746/pdf/ijbmb0013-0049.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40721776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ligand recognition by peptidoglycan recognition protein-S (PGRP-S): structure of the complex of camel PGRP-S with heptanoic acid at 2.15 Å resolution.","authors":"Ankit Maurya,&nbsp;Nabeel Ahmad,&nbsp;Prashant K Singh,&nbsp;Vijayan Viswanathan,&nbsp;Punit Kaur,&nbsp;Pradeep Sharma,&nbsp;Sujata Sharma,&nbsp;Tej P Singh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peptidoglycan recognition proteins (PGRPs) are important components of the innate immune system which provide the first line of defense against invading microbes. There are four members in the family of PGRPs in animals of which PGRP-S is a common domain. It is responsible for the binding to microbial cell wall molecules. In order to understand the mode of binding of PGRP-S to the components of the bacterial cell wall, the structure of the complex of camel PGRP-S (CPGRP-S) with heptanoic acid has been determined at 2.15 Å resolution. The structure determination showed the presence of four crystallographically independent protein molecules which are designated as A, B, C, and D. These four protein molecules associate in the form of two homodimers which are represented as A-B and C-D dimers. The association between molecules A and B gives rise to a shallow cleft on the surface at one end of the dimeric interface. One molecule of heptanoic acid is observed at this binding site in the A-B dimer. The association of C and D molecules results in the formation of a long zig-zag tunnel along with the C-D interface. In the cleft at the C-D interface, three molecules of hydrogen peroxide along with other non-water solvent molecules have been observed. The analysis of the several complexes of CPGRP-S with fatty acids and non-fatty acids such as peptidoglycan, lipopolysaccharide, and lipoteichoic acid shows that the fatty acids bind at the A-B site while non-fatty acids interact through C-D interface.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 4","pages":"28-39"},"PeriodicalIF":0.0,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520249/pdf/ijbmb0013-0028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40392168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and chronic toxicity studies on ethanolic leaf extracts of <i>Clerodendrum viscosum</i> and <i>Leucas indica</i> in Swiss albino mice.","authors":"Rajan Chandrashekar,&nbsp;Mohandas Rai,&nbsp;Bhuvanesh Sukhlal Kalal","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background and objectives: </strong>To evaluate the safe dose range of <i>Clerodendrum viscosum (C. viscosum)</i> and <i>Leucas indica (L. indica)</i> ethanolic leaf extracts of acute and chronic oral toxicity study in Swiss Albino mice.</p><p><strong>Materials and methods: </strong>The Organization for Economic Co-operation and Development guideline was used for the toxicity studies. C. viscosum and L. indica plant extract were administered orally in a single dose of 2000 mg/kg, and general behavior, adverse effects, and mortality were studied for 72 h. For the chronic toxicity study, both plant extracts were administered orally to a separate set of animals at 300 mg/kg doses for 90 days. Animals body weight was taken out, blood and gastric juice were collected for biochemical parameters, and vital organs were collected for histopathological studies after sacrificing test and control group animals.</p><p><strong>Results: </strong>Both in acute and chronic toxicity assay, there was no significant alteration in body weight, physical signs, symptoms, hematological, biochemical parameters, and body organ weights compared to the normal group. The liver, kidney, and stomach histology did not show any drug-induced lesion.</p><p><strong>Conclusions: </strong>The result indicates that the oral administration of <i>C. viscosum</i> and <i>L. indica</i> ethanolic plant extract did not cause any toxicological effects. Hence it could be regarded as a safe natural product for therapeutic use.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 4","pages":"40-48"},"PeriodicalIF":0.0,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520248/pdf/ijbmb0013-0040.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40392167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved method for isolation of RNA from rat femur.","authors":"Alka Khera,&nbsp;Thungapathra MuthuKumarappa,&nbsp;Dheeraj Dumir,&nbsp;Poonam Kanta,&nbsp;Gaurav Kumar,&nbsp;Jaswinder Kalra","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>RNA isolation from ossified bone is a difficult and time-consuming process which often results in poor recovery of RNA. The yield is limited and might not be suitable for gene quantification studies by real time PCR.</p><p><strong>Methodology: </strong>The present study demonstrates RNA extraction from rat femur utilizing the silica column along with the trizol reagent. Quality of RNA was assessed by agarose gel analysis and its suitability for real-time PCR analysis was determined by β-actin Ct values.</p><p><strong>Results: </strong>The RNA isolated using silica columns in conjugation with trizol reagent resulted in higher yield of RNA and purity (A260/280=2.04; yield =1545.73 µg/ml) compared to the trizol method alone (A260/280=1.85; yield =571.2 µg/ml). Ct value of β actin obtained from RNA isolated by trizol method was higher than the Ct value obtained by trizol in conjugation with the column method (31.41 and 15.41 respectively).</p><p><strong>Conclusion: </strong>Combination of trizol along with silica column resulted in better quality and improved yield of RNA suitable for gene quantification by Real time PCR.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 3","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301144/pdf/ijbmb0013-0017.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40634876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of iron deficiency anaemia over glycated hemoglobin in non-diabetic women.","authors":"D'Sa Janice,&nbsp;Mangalore Balakrishna Prathima,&nbsp;Sushith Sushith,&nbsp;Rachana Narayanan,&nbsp;Shridhar Reshma,&nbsp;Suriyan Nair,&nbsp;Bhuvanesh Sukhlal Kalal","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Glycated hemoglobin (HbA1c) is a form of hemoglobin bound to glucose and used as an index of glycaemic control reflecting glucose levels of the previous three months. Iron deficiency anemia (IDA) is the commonest form of anemia that affects HbA1c. Reports on the effects of IDA on HbA1c levels are inconsistent in India. Therefore, the study correlated the HbA1c and IDA in non-diabetic female patients.</p><p><strong>Methods: </strong>A correlative study between HbA1c and IDA was carried out at the Department of Biochemistry, A. J. Institute of Medical Sciences, Mangaluru, India. A total of 50 non-diabetic female patients, aged between 20-50 years, with decreased levels of Hb, MCV and MCHC were selected. Their ferritin levels were determined by ELISA method to confirm IDA. Forty confirmed iron-deficient samples whose serum ferritin levels were <90 pg/dL, were tested for HbA1c levels by nephelometry method.</p><p><strong>Results: </strong>HbA1c correlated positively with serum ferritin, Hb, MCV, MCH and MCHC (P<0.05). There was a significant decrease in mean value of HbA1c in those with severe anemia (4.50±0.34) compared to those with moderate anemia (5.18±0.35) (P<0.001).</p><p><strong>Conclusion: </strong>Results showed positive correlation of HbA1c with ferritin and hemoglobin. Therefore, iron status should be considered during the interpretation of the HbA1c concentrations in diabetes mellitus.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"13 3","pages":"23-27"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301143/pdf/ijbmb0013-0023.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40634877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}