International Journal of Applied Sciences and Biotechnology最新文献_第5页

Antibiotic profiling of Salmonella species isolated from Bagmati River, Kathmandu, Nepal 尼泊尔加德满都Bagmati河沙门氏菌的抗生素分析

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-10-01 DOI: 10.3126/ijasbt.v10i3.47305

N. Kunwar, Manoj Kc, Rashika Shrestha, S. Chaudhary, A. Shrestha, Bibek Rana Chhetri

{"title":"Antibiotic profiling of Salmonella species isolated from Bagmati River, Kathmandu, Nepal","authors":"N. Kunwar, Manoj Kc, Rashika Shrestha, S. Chaudhary, A. Shrestha, Bibek Rana Chhetri","doi":"10.3126/ijasbt.v10i3.47305","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i3.47305","url":null,"abstract":"With rapid population growth, unmanaged urbanization, and industrialization, the holy river Bagmati, has become one of the most contaminated water sources in Kathmandu, Nepal. As a result, the river has lost its capacity for self-purification and became ideal habitat for many pathogenic microorganisms such as E. coli, Salmonella, Vibrio, etc. The study aimed to perform the antibiogram profile of Salmonella species. According to the findings, Salmonella was identified in excessively polluted areas with sewage. Out of total 55 samples, 34.45% were Salmonella positive among which, 10.5% were found to be S. Typhi and S. Paratyphi, and the remaining 79% were found to be other S. enterica serovar. S. enterica serovar was detected in abundance from site B1. Site B2 yielded Salmonella Typhi and Salmonella Paratyphi isolates. Furthermore, Salmonella Paratyphi was isolated from Site B3. The water samples from Site B6 were proven to be Salmonella-free. Antibiotic Susceptibility Test was performed for the positive samples and all the serovars were found sensitive to Amikacin whereas least sensitive to Ampicillin i.e., 86.67%, 100%, and 50% resistant in case of other S. enterica serovar, S. Typhi and S. Paratyphi respectively. All the Salmonella species isolates were sensitive to Ciprofloxacin, Streptomycin, Ofloxacin, and Nitrofurantoin but 100 % resistant to Ampicillin. However, there is not a single data reported as Multi-Drug Resistant Salmonella species in this study. The study emphasized the use of effective treatments against the disease and addresses the health danger to people, animals, and many other living species living nearby the river.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"14 40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80680114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pesticide Persistence in Agriculture and its hazardous effects on Environmental Components 农药在农业中的持久性及其对环境成分的有害影响

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.45095

R. Thapa, Anjal Nainabasti, Ashish Lamsal, Santoshi Malla, B. Thapa, Yushika Subedi, S. Ghimire

引用次数: 1

Enhancing Production Through Input Use Efficiency of Virus-Resistant Pune Selection-3 Papaya Variety 通过投入利用效率提高抗病毒木瓜品种浦那选3号的产量

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.46176

S. Rai, S. Singh, A. Kumar, Apoorva

{"title":"Enhancing Production Through Input Use Efficiency of Virus-Resistant Pune Selection-3 Papaya Variety","authors":"S. Rai, S. Singh, A. Kumar, Apoorva","doi":"10.3126/ijasbt.v10i2.46176","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i2.46176","url":null,"abstract":"An experiment was carried out using virus-resistant papaya variety Pune Selection-3 to study the effect of different cultivation methods for enhanced production and input use efficiency at Horticultural Research Farm, RAU, Pusa during the year 2016-2019. The outcomes of raised bed cultivation (a), drip irrigation (b), fertigation (c), polyethylene mulching (d) along with micronutrient spray (e) were analyzed using different combinations of these parameters as treatments for growth, yield-related parameters, and fruit quality. Nutrient use efficiency was also assessed by examining various interactive components such as soil nutrient content, nutrient acquisition with nutrient content in the leaves, the efficiency of nutrient utilization informs of the biomass produced, fruit yield, and fruit nutritional quality. The plant height was maximum in T1 (155 cm) which was significantly higher than the plant of control treatment. The flowering time was found to be reduced in all treatments. However, total soluble sugar was higher in T3. Overall, when the B:C ratio was compared, the treatment (T1) showed a higher value along with a 2.38-fold fruit yield/plant and 0.89-fold increase in fruit yield per hectare. The nutritional quality of fruits enhanced in T1 treated plants having a 1.78-fold increment in flavonoid content, and 1.2-fold more lycopene. The phenolic content was also 1.8-fold higher than the control (T5). The result obtained in the present study demonstrates that with the implementation of a suitable fertigation system in papaya cultivation we can obtain papaya growth, fruit yield per plant, and fruit quality by targeting enhancement in cost: benefit ratio. \u0000Int. J. Appl. Sci. Biotechnol. Vol 10(2): 84-92.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79130410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild Floristic Diversity of Daman-Simbhanjyang Area, Makwanpur District, Central Nepal 尼泊尔中部Makwanpur地区Daman-Simbhanjyang地区的野生植物多样性

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.46188

Gyanu Thapa Magar, Sujan Chaudhary

{"title":"Wild Floristic Diversity of Daman-Simbhanjyang Area, Makwanpur District, Central Nepal","authors":"Gyanu Thapa Magar, Sujan Chaudhary","doi":"10.3126/ijasbt.v10i2.46188","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i2.46188","url":null,"abstract":"A floristic study of an area is very crucial to determine the status of plant species of particular area or geographical region, as it reflects the whole plant diversity of that area. Our study aimed at identifying the floristic composition of Daman-Simbhanjyang area which included flowering plants, gymnosperms, pteridophytes and bryophytes at wild habitat. The present study has recorded altogether 189 plant species under 163 genera and 90 families. Out of 189 plant species (114 Dicots and 20 Monocots), 134 phanerogams were recorded under 112 genera and 55 families. Asteraceae was found to be the richest family with 14 species among dicots and Orchidaceae (8 spp.) among monocots. Moreover, 30 species of pteridophytes and 22 species of bryophytes were recorded under 28 genera from 15 families and 20 genera from 17 families respectively. However, only three species of gymnosperm were recorded under 3 genera and 3 families at wild habitat. Therefore, the present study has concluded Daman-Simbhanjyang area to be rich in plant species diversity which encompasses many high valued plant species that needs to be conserved and used in sustainable manner. \u0000Int. J. Appl. Sci. Biotechnol. Vol 10(2): 112-123.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89334788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Organic Waste Using Bacillus Species Isolated From Soil 利用土壤中分离的芽孢杆菌降解有机废物

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.44303

Bibek Rana Chhetri, Preksha Silwal, Pooja Jyapu, Yumika Maharjan, Trishana Lamsal, Anup Basnet

{"title":"Biodegradation of Organic Waste Using Bacillus Species Isolated From Soil","authors":"Bibek Rana Chhetri, Preksha Silwal, Pooja Jyapu, Yumika Maharjan, Trishana Lamsal, Anup Basnet","doi":"10.3126/ijasbt.v10i2.44303","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i2.44303","url":null,"abstract":"Organic waste can be enzymatically degraded by microbes. In this study, the Bacillus species were isolated from soil and identified as Bacillus subtilis, Bacillus licheniformis, Bacillus macquariensis, Bacillus brevis, and Bacillus circulans which were optimized considering pH (5, 7, 9) and temperature (37°C, 45°C, 55°C) for the maximum production of amylase, gelatinase, lipase, and cellulase, principally for the degradation of organic waste. The maximum production of amylase was found at 37°C with pH 7 and 9, gelatinase and lipase at 37°C with pH 5,7,9 by almost all identified species. Similarly, the production of cellulase was shown by Bacillus licheniformis only at 45°C, pH 5. The degradation was confirmed by the analysis of the solid content of degraded waste. The maximum degradation of starch and lipid-containing waste was shown by Bacillus macquariensis whereas Bacillus circulans were able to degrade gelatin-containing waste effectively. Bacillus species showed a synergistic effect in biodegradation. Bacillus subtilis and Bacillus licheniformis used in ratios 1:1 and 1:2 were found to be effective degraders of lipid and starch-containing waste respectively. Bacillus macquariensis, Bacillus brevis, and Bacillus circulans used in ratio 1:1:1 showed effective degradation of gelatin-containing waste. The degradation of the organic waste by multi-enzyme producer Bacillus species can be the most effective and eco-friendly method and their optimization for enzyme production can be beneficial for commercial enzyme production as well as for biotechnological applications.\u0000Int. J. Appl. Sci. Biotechnol. Vol 10(2): 104-111.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76268081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earthworm Diversity and Distribution in Kathmandu, Bagmati Province, Central Nepal 尼泊尔中部巴格马蒂省加德满都蚯蚓的多样性和分布

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.45105

A. Singh

{"title":"Earthworm Diversity and Distribution in Kathmandu, Bagmati Province, Central Nepal","authors":"A. Singh","doi":"10.3126/ijasbt.v10i2.45105","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i2.45105","url":null,"abstract":"Earthworms are of ecological and economic significance and are directly related wit human health through biogeochemical cycle. Such a valuable faunal group needs immediate intensified taxonomic treatment in Nepal for their sustainable use, efficient commercial production and biodiversity conservation. Systematic random sampling was used for site selection and hand sorting method was used for sample collection from the field. Collected specimen were photographed in field and preserved in ethanol for lab study with tagging. In total, study reported eight species belonging to two families Lumbricidae and Megascolecidae. External morphological features helped on identification and taxonomic key preparation. Among two sites 6 species were common and abundantly recorded from almost habitats whereas, 1/1 species were different in each site with uncommon reporting from harsh environment. Cultivated crop land with litter and compost was known to be more diverse in species richness than other habitats. Detail taxonomic study and distribution mapping is an essential for Earthworms in Nepal to complete the global database and national soil engineer documentation for organic and healthy environment.\u0000Int. J. Appl. Sci. Biotechnol. Vol 10(2): 124-133.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81853924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Problems of Sweet Orange A Case Study from Pyuthan Municipality, Pyuthan 甜橙的问题识别——以平丹市甜橙为例

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-06-28 DOI: 10.3126/ijasbt.v10i2.46190

A. Adhikari, Bigyan G.C.

{"title":"Identification of Problems of Sweet Orange A Case Study from Pyuthan Municipality, Pyuthan","authors":"A. Adhikari, Bigyan G.C.","doi":"10.3126/ijasbt.v10i2.46190","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i2.46190","url":null,"abstract":"In view of suitable climatic condition in mid hill of Nepal, sweet orange is emerging as a new enterprise for farmers, for which the government of Nepal has also given priority to this crop. In the light of these facts, a case study was conducted in Pyuthan municipality of Pyuthan district to identify the problems related to sweet orange in Pyuthan municipality. All of the farmers had their own land for Sweet orange production. Majority of the respondents (80%) had the annual income below NRs. 20000. Farmers were able to increase their higher education, household expenses, health and savings from Sweet orange production. Transportation, irrigation, citrus greening disorder and green stinky bug were major problem in Sweet orange production in study area. Study suggested that concerned stakeholders should focus their program on quality seedling production, input use, and human resource management, and scientific orchard management, capacity development of farmers, insect pest management, scientific value chain management and post-harvest loss reduction for overall development of sweet orange industry in Pyuthan district. \u0000Int. J. Appl. Sci. Biotechnol. Vol 10(2): 134-139.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"167 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91356628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Improved Amperometric Lactose Biosensor Based on Enzyme Nanoparticles 基于酶纳米颗粒的改进的电流型乳糖生物传感器

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-03-29 DOI: 10.3126/ijasbt.v10i1.44155

J. Ahlawat, V. Aggarwal, R. Jaiwal, C. S. Pundir

{"title":"An Improved Amperometric Lactose Biosensor Based on Enzyme Nanoparticles","authors":"J. Ahlawat, V. Aggarwal, R. Jaiwal, C. S. Pundir","doi":"10.3126/ijasbt.v10i1.44155","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i1.44155","url":null,"abstract":"A desolvation method was used for the synthesis of β-galactosidase (β-GAL) and glucose oxidase (GOD) nanoparticles (NPs) using ethanol. The enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM) and Fourier transform Infra-red spectroscopy (FTIR) and were co-immobilized covalently onto surface of polycrystalline Au electrode. An improved amperometric lactose biosensor was constructed using ENPs (β-GALNPs/GODNPs) modified Au electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode connected through potentiostat. Cyclic voltammetry (CV) and scanning electron microscope (SEM) were used for the study of working electrode before and after immobilization of ENPs. The biosensor showed maximum current at 0.25V within 5s, at pH 6.5 and 25oC. The limit of detection (LOD) was i.e. 1mg/ml and working range was 1-10 mg/mL. The analytical recovery of added lactose (5 and 10 mg/mL) was 94.73±0.5% and 96.4±0.9%, respectively. Coefficient of variation (CV) within and between batch were <3.0 and <4.0 respectively. The biosensor exhibited a good correlation (R2=0.91) between lactose level in milk as measured by standard (enzymatic colorimetric) method and present biosensor. The biosensor was employed for quantification of lactose in milk from human, cow, buffalo and goat. The working electrode lost 50% of its initial activity within 3 months, after its 120 uses, while being stored dry at 4ºC. \u0000Int. J. Appl. Sci. Biotechnol. Vol 10(1): 21-30.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"254 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75048958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluating Potential Importance of Cucumber (Cucumis sativus L. -Cucurbitaceae): A Brief Review 黄瓜潜在重要性评价综述(英文

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-03-29 DOI: 10.3126/ijasbt.v10i1.44152

P. K. Mallick

引用次数: 7

Screening and Confirmation of Vip3 gene presence in Bacillus thuringiensis strains native to Nepal 尼泊尔苏云金芽孢杆菌株中Vip3基因的筛选和确认

International Journal of Applied Sciences and Biotechnology Pub Date : 2022-03-29 DOI: 10.3126/ijasbt.v10i1.44162

Sagar Dahal, Jaishree Sijapati, Sandeep Rijal

引用次数: 0