International Journal of Applied Sciences and Biotechnology最新文献

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Epidemiology of Camel Brucellosis Intended for Export from Kassala State – Eastern Sudan 拟从苏丹东部卡萨拉州出口的骆驼布鲁氏菌病的流行病学
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.48495
Hussein Bashir, Hatim H Abraheem, Maha Ibrahim Khogly, Eslah Abedelhabib Dafaallh, S. H. Idris, Hussam Aldeen Mustafa Abdalrazig Bilal, Y. Shuaib, N. E. Mohammed, M. A. Abdalla
{"title":"Epidemiology of Camel Brucellosis Intended for Export from Kassala State – Eastern Sudan","authors":"Hussein Bashir, Hatim H Abraheem, Maha Ibrahim Khogly, Eslah Abedelhabib Dafaallh, S. H. Idris, Hussam Aldeen Mustafa Abdalrazig Bilal, Y. Shuaib, N. E. Mohammed, M. A. Abdalla","doi":"10.3126/ijasbt.v10i4.48495","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.48495","url":null,"abstract":"Summary\u0000This study was designed to determine the seroprevalence rate of Brucellosis and to identify the risk factors for Brucella infection in camels intended for export from Kassala State. A total of 400 blood samples were collected for serum from all Kassala localities, tested using Rose Bengal Plate test (RBPT) and Competitive Enzyme Linked Immunosorbent Assay C.ELISA). The prevalence rate was (29.5 %) and (27.8%) based on the results of  RBPT and  C.ELISA respectively. According to sex, the prevalence rate of  the disease was (30.4%) out of 335 in males and (24.6%) out of 65 in females. There were no clinical signs of camel's Brucellosis in all tested animals. The prevalence of the disease was (14.4%) in Rural KhashmElgerba, (15.3%) in North Delta, (8.5%) in Wad Elhilau, Rural Kassala (11.9%), Hamashkoreb (12.7%), Rustic Arouma (14.4%), Talkook (16.9%) and Atbra river locality (5.9%) with a significant statistical difference. Herds with more than 20 camels were more frequently affected. In this study the Seroprevalence of Brucella was (9.3 %) in young (1-2 years) and (23.7%) in adult camels (above 5 years). The results of the present study provide the status of seropositivity to Brucella in camels intended for export  in Kassala State and the risk factors that contribute to prevalence of Brucellosis among camels. This situation requires more attention and effort to implement.\u0000Int. J. Appl. Sci. Biotechnol. Vol 10(4): 216-220.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84280500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In-Silico Structural and Functional Characterization of WsMYB44 Protein from Withania somnifera L. Dunal 苦荞麦WsMYB44蛋白的结构与功能分析
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.50850
Lakee Sharma, B. Maurya, S. Rai
{"title":"In-Silico Structural and Functional Characterization of WsMYB44 Protein from Withania somnifera L. Dunal","authors":"Lakee Sharma, B. Maurya, S. Rai","doi":"10.3126/ijasbt.v10i4.50850","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.50850","url":null,"abstract":"The plant based MYB transcription factors have been extensively studied for structural and functional characterization. MYB protein is known to perform several molecular and biological processes including regulation of gene expression. In the current study, we have identified and characterized a R2R3 type MYB TF (WsMYB44) pertaining to root specific library from Withania somnifera. The phylogenetic studies of the identified protein sequence revealed its recent origin from Nicotiana sp., a member of Solanaceae family. Motif scans and domain analysis depicted strong conservation near signature R2 and R3 DNA binding domains which proved the specificity of the WsMYB44 protein. The modeled 3-D structure of protein showed significant binding affinity with the MYB binding DNA element C/TAACG/TG. The synergistic act of the predicted protein was proved by studying protein-protein interaction networks. Furthermore, Gene Ontology terms predicted nuclear localization of protein which was found to be involved in several defense related signaling cascades.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85932860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation on the Role of Different Production Factor on the Yield of Rapeseed 不同生产要素对油菜籽产量影响的评价
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.50901
B. Yadav, P. Wagle, Santosh Rasaily, Nabina B.K., S. Devkota
{"title":"Evaluation on the Role of Different Production Factor on the Yield of Rapeseed","authors":"B. Yadav, P. Wagle, Santosh Rasaily, Nabina B.K., S. Devkota","doi":"10.3126/ijasbt.v10i4.50901","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.50901","url":null,"abstract":"Rapeseed and mustard are third most important edible oilseeds of the world after soybean and palm oil. Among the factor the choice of fertilizer is also one of the major factors as rape seed is very much effective in up taking the nutrient from the soil.  With the objective of finding the judicious use of the combination of chemical fertilizer and other organic fertilizer, experiment was conducted in Oilseed Research program, NARC. The experiment was carried out in randomized complete block design with seven treatments replicated thrice. The plot size was 15 m 2 with plant sown continuously in rows spaced 30 cm apart. Highest yield (396 kg/ha) was observed in treatment no. 7 (125% RDF) Lack of knowledge in the field of the optimum fertilizers use is hindrance in the soil health as well a crop productivity. Best combination of fertilizer and their optimum used can enrich the soil health as well as reduce the input cost and increase the yield. \u0000 ","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"204 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77030912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors Affecting Potato Marketed Surplus in Kavre District of Nepal 影响尼泊尔Kavre地区马铃薯市场盈余的因素
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.50882
M. Kharel
{"title":"Factors Affecting Potato Marketed Surplus in Kavre District of Nepal","authors":"M. Kharel","doi":"10.3126/ijasbt.v10i4.50882","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.50882","url":null,"abstract":"Potato is a staple food crop. It has been being a highly consumed food grown in all parts of Nepal. A study was conducted to identify the factors affecting the potato marketed surplus, taking randomly selected 100 potato growing households in Kavre district of Nepal. Face to face survey with semi structured questionnaire for primary data collection and secondary information was collected through review of relevant publications from the government authority. Descriptive statistics and econometric model were used for data analysis. The multiple linear regression model was used to identify the determinants of potato marketed surplus. It was revealed around 81% of the total potato production was usually sold to the market and the remaining 11% and 9% of potato were used for seed and home consumption respectively. The multiple regression model indicates that among the seven variables used namely area of potato production, NARC released potato varieties, experience of farmer, education level, price and selling to traders were significantly influenced to the marketed surplus of potato in Kavre. Farmers’ access to quality inputs services and capacity building training for enhanced production and strengthening backward and forward linkage for effective marketing. \u0000 ","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84378959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pre-leukemic Cell Detection and Leukemic Transformation of a Normal Marrow Cell: A Mini review 白血病前细胞检测和正常骨髓细胞的白血病转化:一个小综述
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.49499
Bibek Rana Chhetri, R. Thapa, D. Shrestha, Agrani Paudel, Sandesh Adhikari, Arjun Gautam
{"title":"Pre-leukemic Cell Detection and Leukemic Transformation of a Normal Marrow Cell: A Mini review","authors":"Bibek Rana Chhetri, R. Thapa, D. Shrestha, Agrani Paudel, Sandesh Adhikari, Arjun Gautam","doi":"10.3126/ijasbt.v10i4.49499","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.49499","url":null,"abstract":"The hematopoietic cells are multipotent primitive cells, which differentiate into either common myeloid and lymphoid progenitor. However, if there an abnormality in this process of differentiation, condition of leukemia arises, which is the 11th leading cause of cancer-related mortality worldwide in the year 2018. These abnormalities are brought about by array of mutations occurring at cellular level. According to the two-hit model hypothesis, key oncogenic events are classified into two classes: class I mutations and class II mutations. Class I  mutations are those that causes activation of the receptor tyrosine kinase (RTK), FLT3, c-kit (KIT), and Ras signaling pathways thereby increasing proliferation rate of progenitor cells. Class II mutations include recurrent chromosomal abnormalities such as t(8; 21), inv(16), and t(15; 17), which result in fusion transcripts of RUNX1/ETO, CBF/MYH11, and PML/RAR, respectively that eventually impair hematopoietic differentiation. The factors associated with leukemia can be biological, chemical or socio-economical. The advancement in the researches on the topic have aided to the development of various technologies such as detection of DNMT3A and xenografts assays, in order to detect these mutations in pre-leukemic cells. This review aims to provide an introduction to the condition, its types and provide brief summary on genes and mutations responsible for the condition. The factors associated with leukemia and technologies involved in the detection of leukemia are also reviewed.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90232820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance Evaluation of Papaya Genotypes in Nepal 尼泊尔木瓜基因型的性能评价
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-12-30 DOI: 10.3126/ijasbt.v10i4.50883
JN Chaudhary, IP Gautam, YR Bhusal, T. Gotame
{"title":"Performance Evaluation of Papaya Genotypes in Nepal","authors":"JN Chaudhary, IP Gautam, YR Bhusal, T. Gotame","doi":"10.3126/ijasbt.v10i4.50883","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i4.50883","url":null,"abstract":"Coordinated varietal trials (CVTs) were conducted to identify the high yielding papaya (Carica papaya L.) genotypes at different agro- climatic conditions with eight genotypes at Agriculture Research Station (ARS), Malepatan, Pokhara; Viuran, Nawalparasi and Parewatar, Dhading during 2006-2009 and seedling were transplanted at the time of October and experiment was designed in RCBD and replicated thrice. Observations were recorded on biological and socio-economic parameters and analyzed. The pooled data over year revealed that number of fruits, marketable yield, average weight and circumference of the fruits at ARS, Malepatan and marketable fruit yield and average weight of the fruit at Viuran, Nawalparasi and Parewatar, Dhading showed the highly significant differences among the tested genotypes. Days to first harvest and length of the fruits at ARS, Malepatan and days to flowering, first harvest and number of fruits per plant at Viuran, Nawalparasi and Parewatar, Dhading showed the significant differences within genotypes. While, non-significant difference was noticed under days to flowering at ARS, Malepatan. Genotypes Red Lady was found early in all location (295 days to harvest) and the highest production of the marketable fruit yield (104.22 t/ha) followed by Farm selection-1 (102.37 t/ha) and which was at par with each other. Farm selection-1 recorded in the highest number of fruits per plant (41.4), the average fruit weight (1.46 kg) followed by Red Lady (37 and 1.37 kg at ARS, Malepatan, Pokhara. Farmers and consumers preferred red Lady due to earliness, red flesh color and good storability. \u0000Int. J. Appl. Sci. Biotechnol. Vol 10(4): 221-224.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79179137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Invitro Efficacy of Trichoderma Isolates and Commercial Fungicides against Exobasidium vexans, Causal Agent of Blister Blight in Tea 分离木霉与市售杀菌剂对茶叶水疱病病原菌vexaobasidium的体外防治效果研究
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-10-01 DOI: 10.3126/ijasbt.v10i3.47524
Karun Adhikari, A. Rai
{"title":"Invitro Efficacy of Trichoderma Isolates and Commercial Fungicides against Exobasidium vexans, Causal Agent of Blister Blight in Tea","authors":"Karun Adhikari, A. Rai","doi":"10.3126/ijasbt.v10i3.47524","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i3.47524","url":null,"abstract":"Blister blight is one of the most significant foliar fungal diseases of tea plant caused by Exobasidium vexans. An invitro experimentation on efficacy of biofungicides and chemical fungicides was carried out at Agriculture Research Station, Pakhribas from November 2020 to January 2021 to evaluate and analyze effectivity of two Trichoderma spp and five commonly used chemical fungicides against Blister Blight pathogen. Isolates of Trichoderma viride and Trichoderma harzianum were tested against E. vexans using dual culture technique. Five Chemical fungicides namely Copper oxychloride 50WP, Hexaconazole 5% EC, Metalaxyl 8% WP+ Mancozeb 64% WP, Carbendazim 50% WP, Carboxin 37.5% WS + Thiram 37.5% WS were tested at four different concentrations viz. 50 ppm, 100 ppm, 200 ppm and 500 ppm using poisoned food technique. The experiment was carried out in completely randomized design with three replications for each treatment. Both Trichoderma viride and Trichoderma harzianum demonstrated significant effect on mycelial growth reduction of E. vexans. Trichoderma viride and Trichoderma harzianum exhibited 70.87% and 66.98% inhibition in growth of E. vexans respectively. Similarly, Hexaconazole, Carbendazim, Carboxin + Thiram provided complete (100%) inhibition on pathogen growth regardless of concentration. Least inhibition (36%) on pathogen growth was recorded at 50 ppm of Copper Oxychloride. All chemical fungicides provided significant difference on reducing growth of pathogen in comparison to control at all concentration. It is recommended that Trichoderma spp be taken as a priority regarding its environmental benefit and furthermore if application of chemical fungicides is necessitated, rationalized use be done at lower dose and with appropriate timing.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86505106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Pruning and Fertilizers on Growth, Flowering and Yield of Cucumber (Cucumis sativus L.) under Protected Structure in Panchthar, Nepal 修剪和施肥对尼泊尔Panchthar保护地黄瓜生长、开花和产量的影响
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-10-01 DOI: 10.3126/ijasbt.v10i3.47521
Upama Chapagain, Sujan Bhandari, Yogendra Shrestha, Sagar Bista, Januka Dahal
{"title":"Effect of Pruning and Fertilizers on Growth, Flowering and Yield of Cucumber (Cucumis sativus L.) under Protected Structure in Panchthar, Nepal","authors":"Upama Chapagain, Sujan Bhandari, Yogendra Shrestha, Sagar Bista, Januka Dahal","doi":"10.3126/ijasbt.v10i3.47521","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i3.47521","url":null,"abstract":"A study was conducted to determine the effect of pruning practices and different fertilizer combinations on Bhaktapur local variety of cucumber under protected structure in Panchthar, Nepal during February to June 2021. The experiment was laid out in two factorial Randomized Complete Block Design with three replications. Treatments used were two pruning practices (no pruning and 3G cutting) and four fertilizer doses (no fertilizer (control), 30 Mt/ha FYM+ 100% RDF (140:60:100 kg of NPK), 9.4 Mt/ha Vermicompost+ 100% RDF of NPK and 4.3 Mt/ha Poultry manure+ 100% RDF of NPK). Although 3G cutting practice increased the days required for 50 % flowering, it significantly decreased the male: female flowers ratio (1.69) compared to no pruning (4.38). Similarly, number of fruits per plant, individual fruit weight, fruit diameter and average fruit yield (95.21 Mt/ha) were maximum in 3G cutting. In terms of fertilizers, time requirement for 50% male flowering remained unaffected, however, plants treated with fertilizers 30 Mt/ha FYM+ 100% RDF of NPK showed significantly least days required for 50% female flowering, lowest male: female flowers ratio (2.41), maximum number of fruits per plant, maximum average individual fruit weight and highest average yield (105.78 Mt/ha) which are statistically at par with results of 9.4 Mt/ha vermicompost+ 100% RDF of NPK and 4.3 Mt/ha poultry manure+ 100% RDF of NPK for these parameters. The results concluded that combined application of organic manures and inorganic fertilizers in recommended dose with 3G pruning practices is best for growth and yield of cucumber.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73251870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of Heavy Metals in Vegetables from Contaminated Agricultural Soils of Madhyapur Thimi, Bhaktapur District, Nepal and their Potential Health Risk Assessment 尼泊尔中央区农业土壤中蔬菜重金属含量评价及潜在健康风险评价
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-10-01 DOI: 10.3126/ijasbt.v10i3.48703
Anuska Sharma, B. Adhikari, M. Shrestha, Dipesh Raj Pant, Bindra Devi Shakya, Achut Ram Pradhananga, Sudarshana Shakya, Pawan Raj Shakya
{"title":"Evaluation of Heavy Metals in Vegetables from Contaminated Agricultural Soils of Madhyapur Thimi, Bhaktapur District, Nepal and their Potential Health Risk Assessment","authors":"Anuska Sharma, B. Adhikari, M. Shrestha, Dipesh Raj Pant, Bindra Devi Shakya, Achut Ram Pradhananga, Sudarshana Shakya, Pawan Raj Shakya","doi":"10.3126/ijasbt.v10i3.48703","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i3.48703","url":null,"abstract":"The present study aimed to determine Cd, Cr, Cu, Pb, and Zn concentrations using flame atomic absorption spectrophotometer (FAAS) in five vegetables viz., Coriander (Coriandrum sativum), Mustard (Brassica campestris), Radish (Raphanus sativus), Spinach (Spinacea oleracea) and Chinese spinach (Amaranthus dubius), and their growing soils in three agricultural sites (Manohara, Bode, and Nagadesh) of Madhyapur Thimi, Bhaktapur district and to assess health risks using USEPA deterministic approaches. The average concentrations of HMs in soils were 0.85, 30.65, 40.06, 47.42, and 129.55 mg/kg for Cd, Cr, Cu, Pb, and Zn respectively, exceeding the normal soil quality standards except Cr. Similarly, the average concentrations of HMs in vegetables were 0.42, 6.63, 22.33, 1.34, and 77.16 mg/kg for Cd, Cr, Cu, Pb, and Zn respectively exceeding joint FAO/WHO standards except for Cu. The I-geo values revealed a class of unpolluted to moderately polluted levels for the agricultural soils indicating the 1st degree of soil pollution. Among the vegetables, Chinese spinach measured considerably high transfer factor (TF) for Cd (0.79), Cr (0.34), Cu (0.76), Pb (0.04), and Zn (0.89) indicating health concerns to the consumers. The hazard index (HI) for these HMs was found less than the acceptable limit (1.0) indicating no non-carcinogenic risk to adults through vegetable consumption. However, the lifetime carcinogenic risk (LCR) parameter indicated low to high cancer risk for Cd, Cr, and Pb. Among the vegetables, Chinese spinach estimated the highest LCR values for the HMs suggesting regular monitoring of HMs in soil and vegetable on account of their toxic effects.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85931511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibiotic profiling of Salmonella species isolated from Bagmati River, Kathmandu, Nepal 尼泊尔加德满都Bagmati河沙门氏菌的抗生素分析
International Journal of Applied Sciences and Biotechnology Pub Date : 2022-10-01 DOI: 10.3126/ijasbt.v10i3.47305
N. Kunwar, Manoj Kc, Rashika Shrestha, S. Chaudhary, A. Shrestha, Bibek Rana Chhetri
{"title":"Antibiotic profiling of Salmonella species isolated from Bagmati River, Kathmandu, Nepal","authors":"N. Kunwar, Manoj Kc, Rashika Shrestha, S. Chaudhary, A. Shrestha, Bibek Rana Chhetri","doi":"10.3126/ijasbt.v10i3.47305","DOIUrl":"https://doi.org/10.3126/ijasbt.v10i3.47305","url":null,"abstract":"With rapid population growth, unmanaged urbanization, and industrialization, the holy river Bagmati, has become one of the most contaminated water sources in Kathmandu, Nepal. As a result, the river has lost its capacity for self-purification and became ideal habitat for many pathogenic microorganisms such as E. coli, Salmonella, Vibrio, etc. The study aimed to perform the antibiogram profile of Salmonella species. According to the findings, Salmonella was identified in excessively polluted areas with sewage. Out of total 55 samples, 34.45% were Salmonella positive among which, 10.5% were found to be S. Typhi and S. Paratyphi, and the remaining 79% were found to be other S. enterica serovar. S. enterica serovar was detected in abundance from site B1. Site B2 yielded Salmonella Typhi and Salmonella Paratyphi isolates. Furthermore, Salmonella Paratyphi was isolated from Site B3. The water samples from Site B6 were proven to be Salmonella-free. Antibiotic Susceptibility Test was performed for the positive samples and all the serovars were found sensitive to Amikacin whereas least sensitive to Ampicillin i.e., 86.67%, 100%, and 50% resistant in case of other S. enterica serovar, S. Typhi and S. Paratyphi respectively. All the Salmonella species isolates were sensitive to Ciprofloxacin, Streptomycin, Ofloxacin, and Nitrofurantoin but 100 % resistant to Ampicillin. However, there is not a single data reported as Multi-Drug Resistant Salmonella species in this study. The study emphasized the use of effective treatments against the disease and addresses the health danger to people, animals, and many other living species living nearby the river.","PeriodicalId":13876,"journal":{"name":"International Journal of Applied Sciences and Biotechnology","volume":"14 40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80680114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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