International Journal of Angiology最新文献

{"title":"Cumulative Serum Endothelin-1 and Endothelin-3 Level Predicts 1-year Adverse Cardiac Events after ST-Elevation Acute Myocardial Infarction.","authors":"Astrid Karina Putri, Anggoro Budi Hartopo, Ira Puspitawati, Budi Yuli Setianto","doi":"10.1055/a-2572-0833","DOIUrl":"10.1055/a-2572-0833","url":null,"abstract":"<p><p>Endothelin-1 (ET-1) and endothelin-3 (ET-3) released in circulation during acute myocardial infarction (AMI) contribute to vasoconstriction, which elevates the severity of the myocardial infarction. This study aims to investigate the role of cumulative ET-1 and ET-3 levels on the major adverse cardiac events (MACE) 1 year after AMI hospitalization. This retrospective cohort study included patients with ST-elevation acute myocardial infarction (STEMI). Baseline data were collected, including sociodemographic characteristics, clinical data, and serum ET-1 and ET-3 levels, measured with ELISA assay. Follow-up was performed 1 year after hospital discharge to identify MACE. A multivariate logistic regression was performed to describe predictors of 1-year MACE. A total of 81 subjects were enrolled and followed up. Subjects were divided into two groups based on their cumulative ET-1 and ET-3 levels, using a rounded cut-off point of 17.0 ng/mL, produced by the ROC curve for MACE. At 1 year MACE occurred in 24 (29.3%) subjects. A multivariate analysis demonstrated that a cumulative ET-1 and ET-3 level >17.0 ng/mL was independently associated with 1-year MACE (aOR = 5.34, 95% CI: 1.36-21.07, <i>p</i>  = 0.017). A higher cumulative serum ET-1 and ET-3 level at admission predicts MACE in 1 year of follow-up after STEMI.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"35 1","pages":"43-49"},"PeriodicalIF":0.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Endoaortic Balloon Occlusion and Transthoracic Aortic Clamp for Minimally Invasive Cardiac Surgery: Systematic Review and Meta-analysis.","authors":"Noritsugu Naito, Hisato Takagi","doi":"10.1055/a-2572-1060","DOIUrl":"10.1055/a-2572-1060","url":null,"abstract":"<p><p>This meta-analysis aimed to compare short-term outcomes in patients undergoing minimally invasive cardiac surgery (MICS) using endoaortic balloon (EAB) and transthoracic aortic clamp (TAC) techniques. A comprehensive search of relevant databases was conducted through July 2024. Pooled results were calculated, and subgroup analyses of studies published during early and late eras were performed. Additionally, a meta-regression analysis based on the year of publication was conducted to assess potential influences on outcomes. The systematic review identified 17 non-randomized studies encompassing a total of 8,253 patients. In terms of intraoperative outcomes, no significant differences were observed in operation duration, aortic cross-clamp time, cardiopulmonary bypass time, or rates of sternotomy conversion and iatrogenic aortic dissection. However, subgroup analysis of early-era studies demonstrated significantly longer operation times in the EAB cohort ( <i>p</i>  = 0.03). Meta-regression analysis indicated that the standardized mean differences in cardiopulmonary bypass time between the two groups decreased in favor of EAB as publication years progressed ( <i>p</i>  = 0.01). For postoperative outcomes, no significant differences were found in postoperative stroke rate, the rate of reoperation for bleeding, or length of hospital stay. However, the EAB group had significantly lower rates of postoperative atrial fibrillation (OR = 0.82 [0.70-0.95], <i>p</i>  < 0.01) and short-term mortality (OR = 0.60 [0.39-0.92], <i>p</i>  = 0.04). This meta-analysis demonstrated that EAB is associated with perioperative outcomes comparable to TAC in select patients. The cumulative experience and evolution of techniques may have contributed to improved outcomes with EAB over time.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 3","pages":"176-190"},"PeriodicalIF":0.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coronary Artery Disease and Loneliness: A Cross-Sectional Analysis using the Revised UCLA Loneliness Scale.","authors":"Tanzim Bhuiya, Jonathan H Sussman, Francis O'Neill, Tashina Dussie, Gerard Dichiara, Joseph Abraham, Roman Zeltser, Amgad N Makaryus","doi":"10.1055/a-2552-8626","DOIUrl":"10.1055/a-2552-8626","url":null,"abstract":"<p><strong>Background: </strong>Loneliness has been identified as an important psychosocial factor associated with cardiovascular disease, but the relationship has been underexplored using validated measures.</p><p><strong>Materials and methods: </strong>This cross-sectional study analyzed 92 patients from 2018 to 2019 using the Revised UCLA Loneliness Scale to evaluate associations between loneliness and coronary artery disease (CAD). Statistical analysis was performed using R v4.4.0. A multivariate logistic regression model assessed the relationship between loneliness scores and CAD, adjusting for age and race/ethnicity. A one-sided Wilcoxon rank-sum test compared loneliness scores between CAD and non-CAD patients.</p><p><strong>Results: </strong>Age was significantly associated with CAD (odds ratio [OR]: 1.05, 95% confidence interval [CI]: 1.01-1.08, <i>p</i>  = 0.0084), whereas loneliness score showed a trend toward significance (OR: 1.03, 95% CI: 0.99-1.07, <i>p</i>  = 0.140). No significant associations were found between race/ethnicity or sex and loneliness. In a subgroup analysis of patients ≥ 55 years, loneliness was significantly associated with CAD (OR: 1.06, 95% CI: 1.00-1.12, <i>p</i>  = 0.04), whereas age was not ( <i>p</i>  = 0.378). Patients aged ≥ 55 years with CAD had significantly higher loneliness scores than those without CAD ( <i>p</i>  = 0.044), whereas no significant difference was observed in patients < 55 years ( <i>p</i>  = 0.87).</p><p><strong>Conclusion: </strong>While loneliness was not independently associated with CAD in the overall cohort, it was significantly associated with CAD in patients aged ≥ 55 years. This suggests that loneliness may be a relevant factor in cardiovascular health among older adults. This emphasizes the need for health care providers to consider loneliness as a potential risk factor for CAD, alongside traditional risk factors.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"35 2","pages":"83-89"},"PeriodicalIF":0.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13171201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147941373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aortic Atherosclerosis: An Updated Review of Current Guidelines and Management Approach-A Literature Review.","authors":"Mohamad Mubder, Jasmine Dugal, Nazanin Houshmand, Tahir Tak","doi":"10.1055/a-2536-9215","DOIUrl":"10.1055/a-2536-9215","url":null,"abstract":"<p><p>Aortic atheroma (AA) is defined by the development of atherosclerotic plaques within the aorta wall and is associated with unfavorable cardiovascular outcomes such as cryptogenic stroke, systemic embolism, and other major adverse cardiac events (MACE). The clinical significance of AA is discussed in this review along with its relationship to cardiovascular outcomes. The purpose of this review article is to present a thorough summary of the current state of knowledge on AA, including its pathogenesis, classifications and grading systems, clinical implications, methods for diagnosis and imaging, therapeutic modalities and treatments, and lastly pertinent complications. There are various methods of AA classification or grading, which entail scoring extent of disease via plaque thickness, severity of atherosclerosis, or by the presence of mobile components or ulcerations. The implications of AA as a systemic atherosclerotic process on the development of coronary artery disease and MACE include, but are not limited to, vascular diseases such as strokes or peripheral arterial disease. The diagnostic approaches include multiple invasive and noninvasive imaging modalities. The treatment strategies range from prevention to medical therapy to invasive surgical interventions. The present evaluation highlights the significance of sustained investigation and advancement in the domain of aortic atherosclerosis to augment precision in diagnosis and effectiveness of treatment.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"35 1","pages":"25-36"},"PeriodicalIF":0.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Left Ventricular Assist Device in the Management of Heart Failure: A Single-Center Experience.","authors":"Sam Karem, Rajasekhar S R Malyala, Sibu Saha","doi":"10.1055/a-2536-9161","DOIUrl":"10.1055/a-2536-9161","url":null,"abstract":"<p><strong>Background: </strong>Heart failure is a prevalent health care issue in the United States. While most cases of heart failure can be managed medically, intractable cases benefit from a left ventricular assist device (LVAD).</p><p><strong>Aim: </strong>The aim of this study is to review the outcomes of LVAD therapy at the University of Kentucky.</p><p><strong>Materials and methods: </strong>Data received from University of Kentucky Hospital. We received Institutional Review Board approval to review patient records admitted for LVAD implantation from January 1, 2017, to December 31, 2021.</p><p><strong>Results: </strong>After reviewing records, we had 127 eligible LVAD patients with an age range of 18 to 83 years, with 87% of recipients being white, 12.5% being black, 81% male, and 19% female. Results show the average length of admission for patients receiving LVAD is 41 days. Quality of life is reported using the New York Heart Association (NYHA) classification at follow-up, which shows 6 patients in Class I, 52 patients progressing to Class II, 21 patients to class III; other patients were deceased before discharge or did not go below class IV heart failure. Prior to 2021, there were 105 patients and 65 patients were alive at 2 years follow-up, and 18 out of 23 patients who received LVAD after 2021 are still living. For postoperative complications, 26% developed stroke, 24.4% developed gastrointestinal bleeding, 26% developed renal failure, 44.8% developed respiratory failure, 37% developed driveline infection, 14.2% developed right ventricular dysfunction/failure, and 20.4% developed an LVAD complication.</p><p><strong>Conclusion: </strong>LVAD has been implemented to manage advanced heart failure and improve quality of life.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 3","pages":"220-225"},"PeriodicalIF":0.8,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for Postoperative Intimal Hyperplasia.","authors":"Mitsuhiro Yamamura, Taichi Sakaguchi, Hiroe Tanaka, Ken-Ichi Watanabe, Hisashi Uemura, Yuji Sakashita, Yoshio Teshima, Masashi Bungo","doi":"10.1055/a-2524-1844","DOIUrl":"10.1055/a-2524-1844","url":null,"abstract":"<p><p>Interleukin-8 (IL-8) is a neutrophil chemotactic factor produced by vascular endothelial cells. This study aimed to evaluate whether the serum IL-8 level can be the biomarker for postoperative intimal hyperplasia in the rat model. Sixteen male Lewis rats (mean weight: 501 ± 44 g) were subjected in this study. The right epigastric vein graft is interposed into the common femoral artery with 10-0 interrupted sutures, as previously described. Vein grafts from each group were stained with hematoxylin and eosin and Elastica Verhoeff's van Gieson's at 2 and 4 weeks postoperatively. Unoperated right epigastric veins were also examined as a Sham. In Group edaravone, free-radical scavenger, edaravone (Radicut ^® , Mitsubishi Tanabe Pharma Corp., Osaka Japan) was administered, instead of saline. The initial areas of vein grafts were measured using computerized planimetry (NIH Image Ver. 1.61). Serum IL-8 levels were measured and compared using an unpaired <i>t</i> -test. This study was approved by the Hyogo College of Medicine Animal Research Committee (No. 235). The average intimal area at 4 weeks in Control group was significantly increased compared with that of Sham group (0.43 ± 0.11 vs. 0.00 ± 0.00 mm ² , <i>p</i>  < 0.01). The average serum IL-8 levels at 4 weeks were also significantly increased, compared with that of Sham group (148 ± 6 vs. 53 ± 14 pg/mL, <i>p</i>  < 0.05). The serum IL-8 levels in Group edaravone were appeared to be suppressed but not significant (132 ± 24 pg/mL, <i>p</i>  = 0.72). It is very important to detect the biomarker such as the serum IL-8, before the establishment of postoperative intimal hyperplasia.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 2","pages":"164-166"},"PeriodicalIF":0.8,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal Trends in Pulmonary Embolism Mortality Associated with COVID-19 in the United States: A 5-Year Retrospective Analysis.","authors":"Giuseppe Lippi, Camilla Mattiuzzi","doi":"10.1055/a-2534-2520","DOIUrl":"10.1055/a-2534-2520","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19) has been strongly associated with thrombotic complications, particularly pulmonary embolism (PE). This study aims to analyze the real-world impact of COVID-19 on PE-related mortality in the United States over the first 5 years of the pandemic. We conducted a retrospective observational study using the CDC WONDER database, combining data on mortality from PE and COVID-19 using the 10th revision of the International Classification of Diseases (ICD-10) codes I26 (PE) and U07.1 (COVID-19). The analysis spanned from 2020 to 2024. We found a peak in COVID-19-associated PE deaths in 2021, with an estimated crude rate of 211 × 1,000 PE deaths. This rate progressively declined over the following years, being 141 × 1,000 in 2022, 44 × 1,000 in 2023, and 29 × 1,000 in 2024, respectively. The estimated crude rate for COVID-19-associated PE in the United States was 111 × 1,000 PE deaths throughout the 5-year period. The results of our study show that COVID-19 may have contributed to nearly one-tenth of all PE deaths throughout the first 5 years of the pandemic. Despite the gradual decline over the years since its peak in 2021, the still sustained mortality suggests ongoing thrombotic risks, especially impacting high-risk populations, emphasizing the need for continued vigilance and prevention of thrombotic complications in COVID-19 patients.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 3","pages":"211-214"},"PeriodicalIF":0.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Left and Right Branch Blocks with Clinical Outcomes in Patients with Heart Failure with Preserved Ejection Fraction.","authors":"Sukhila Reddy, Lakshmi Kattamuri, Alok Dwivedi, Debabrata Mukherjee, Abhizith Deoker","doi":"10.1055/a-2516-2082","DOIUrl":"10.1055/a-2516-2082","url":null,"abstract":"<p><p>The associations of left bundle branch block (LBBB) and right bundle branch block (RBBB) with cardiovascular (CV) outcomes compared with normal QRS in heart failure patients with preserved ejection fraction (HFpEF) are unclear. We sought to determine CV morbidity, mortality, and total costs associated with LBBB and RBBB in acute HFpEF compared with those without BBB. A cross-sectional study using the 2019 NIS database analyzed adult HFpEF hospitalizations for acute heart failure, categorizing patients by LBBB, RBBB, or normal QRS. Clinical outcomes included atrial fibrillation (AF), ventricular fibrillation (VF), complete heart block (CHB), sick sinus syndrome (SSS), cardiac arrest (CA), hospitalization costs, and in-hospital mortality. Associations between BBB types and outcomes were assessed using logistic and relative risk (RR) models with propensity score weighting, validated by sensitivity analyses. A total of 334,511 hospitalizations with HFpEF including LBBB (6,818, 2%) and RBBB (7,948, 2.4%) were analyzed. Compared with normal QRS duration, LBBB was associated with VF (odds ratio [OR] = 2.47, <i>p</i>  < 0.001), SSS (OR = 1.72, <i>p</i>  < 0.001), CHB (OR = 2.77, <i>p</i>  < 0.001) and greater hospitalization costs (RR = 1.15, <i>p</i>  < 0.001) after adjusting for covariates in PS analysis. Similarly, RBBB was associated with AF (OR = 1.30, <i>p</i>  < 0.001), VF (OR = 1.59, <i>p</i>  = 0.033), SSS (OR = 1.72, <i>p</i>  < 0.001), CHB (OR = 2.81, <i>p</i>  < 0.001), CA (OR = 1.19, <i>p</i>  < 0.001), and higher hospitalization costs (RR = 1.08, <i>p</i>  < 0.001). These associations remained unchanged in multiple validation analyses even after additionally adjusting for obesity, hypertension, and diabetes. The length of stay was shorter in HFpEF with RBBB and LBBB subgroups compared with normal QRS. In hospitalizations of acute decompensated HFpEF, BBB was associated with an increased risk of CV outcomes and hospitalization costs.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 2","pages":"92-99"},"PeriodicalIF":0.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aorta Rupture Due to Extracorporeal Shock Wave Lithotripsy: A Rare Case Report.","authors":"Christos Stagkoglou, Elias Kaperonis, Vasileios Papavasileiou","doi":"10.1055/a-2516-2038","DOIUrl":"10.1055/a-2516-2038","url":null,"abstract":"<p><p>Since the 1980s, extracorporeal shock wave lithotripsy (ESWL) has been the treatment of choice for most urinary calculi. It is generally considered a safe method with a few complications involving the vascular system, among others. There are only a few literature reports of aortic rupture after ESWL and these mainly concern abdominal aortic aneurysms and rarely, severe atherosclerotic aorta. We report the case of a 67-year-old man with a rupture of a longitudinally and circumferentially calcified abdominal aorta and pseudoaneurysm formation following ESWL which he underwent a few days before the symptoms started. He reported urgently to our department after a recurrence of severe abdominal and lumbar pain. An endovascular treatment attempt with stent graft implantation was made initially but since the perforation did not seal, open surgery with stent removal, hematoma excision, and an aortobifemoral bypass was decided. He died 23 days later because of acute myocardial infarction. We believe that it is possible for severely calcified vessels to rupture through ESWL, and great care should be taken in these patients for symptoms or signs of retroperitoneal hematoma after the procedure.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 2","pages":"159-163"},"PeriodicalIF":0.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA Profiling in Chronic Limb-Threatening Ischemia Their Role in Arteriogenesis.","authors":"Muhamad T Ismail, Budi Y Setianto, Sofia M Haryana, Dyah W Anggrahini","doi":"10.1055/a-2504-1911","DOIUrl":"10.1055/a-2504-1911","url":null,"abstract":"<p><p>Chronic limb-threatening ischemia (CLTI) represents poses a substantial threat with escalating mortality and amputation rates. Despite the existence of various clinical techniques for diagnosing CLTI, the role of microRNAs (miRNAs) in arteriogenesis remains ambiguous. Comprehensive knowledge on miRNAs may facilitate the advancement of targeted therapy pertaining to the enhancement of collateral blood flow in obstructed vessels. Therefore, this study aimed at analyzing arteriogenesis-associated plasma miRNA profiles in patients with CLTI using gene expression. Samples were acquired from the collateral arteries (CA group, <i>n</i>  = 3) and the contralateral healthy limb (healthy artery; HA group, <i>n</i>  = 3) of a single set of patients with CLTI. The RNA extracted from the samples was assessed for concentration and purity. A normalization factor was used to address variations in analyte abundance and/or quality across the samples. Subsequently, individual RNA molecules were directly quantified and subjected to comparative analysis between the CA and HA groups to identify the miRNAs involved in arteriogenesis. The five arteriogenesis-related miRNAs exhibiting maximum upregulation were miR-301b-3p, miR-221-5p, miR-639, miR-34a-5p, and let-7a-5p, while the five most downregulated miRNAs included miR-151a-5p, miR-371a-5p, miR-651-5p, miR-510-5p, and miR-660-5p. Summarily, this study documented marked upregulation and downregulation of miRNAs associated with arteriogenesis in the collateral arteries of patients with CLTI as compared with their contralateral healthy limbs. Possible mechanisms involved, including the regulation of YAP/TAZ pathway, TGFBR3 mRNA, SIRT1 expression, and other processes have shown to be modulated by miRNAs fluctuations.</p>","PeriodicalId":13798,"journal":{"name":"International Journal of Angiology","volume":"34 2","pages":"83-91"},"PeriodicalIF":0.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}