International Journal of Bioassays最新文献_第5页

Mycorrhizal association and influence on growth of Asian pigeonwings (Clitoria ternatea L.) 菌根结合力及其对亚洲鸽子生长的影响

International Journal of Bioassays Pub Date : 2017-06-02 DOI: 10.21746/IJBIO.2017.06.006

S. R. Mane, V. R. Kumbhar, G. M. Birajdar, R. S. Naryankar, M. T. Gavali, U. Bhale

引用次数: 1

Implication of blue green algae on yield attributes and economics of rice cultivation 蓝藻对水稻产量属性和经济的影响

International Journal of Bioassays Pub Date : 2017-06-01 DOI: 10.21746/IJBIO.2017.06.001

R. Mallick

引用次数: 0

Cadmium-induced alterations in bio-chemical constituents of Labeo rohita and Cyprinus carpio fry: A comparative study 镉对鲫鱼鱼苗生化成分影响的比较研究

International Journal of Bioassays Pub Date : 2017-06-01 DOI: 10.21746/IJBIO.2017.06.002

S. Vadlamani, C. K. Basuri, P. R. Yallapragada

引用次数: 2

Efficacy of AM fungi against drought stress on sweet corn cultivars with special reference to biochemical contents AM真菌对甜玉米品种抗旱性的影响，特别参考生化含量

International Journal of Bioassays Pub Date : 2017-06-01 DOI: 10.21746/IJBIO.2017.06.004

Neelima Singh, B. Shinde

引用次数: 5

Comparative evaluation of Optical Coherence Tomography (OCT) with Pattern Reversal Visual Evoked Potential (PRVEP) in early primary open angle glaucoma 光学相干断层扫描(OCT)与模式反转视觉诱发电位(PRVEP)在早期原发性开角型青光眼中的比较评价

International Journal of Bioassays Pub Date : 2017-06-01 DOI: 10.21746/IJBIO.2017.06.003

A. Taksande, A. Rawekar, P. Sune

{"title":"Comparative evaluation of Optical Coherence Tomography (OCT) with Pattern Reversal Visual Evoked Potential (PRVEP) in early primary open angle glaucoma","authors":"A. Taksande, A. Rawekar, P. Sune","doi":"10.21746/IJBIO.2017.06.003","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.06.003","url":null,"abstract":"Present study is to correlate the average retinal nerve fiber layer (RNFL) thickness and the visual function evaluated by electrophysiologic retinal and cortical responses assessed in primary open-angle glaucoma (POAG) eyes by a Case-control study. One hundred and sixty-one control and glaucoma patients (mean age, 55.18±5.19 years for study group and 54.45±4.81 years for control group) were selected in the study . Average Retinal Nerve fiber layer thickness was measured by optical coherence tomography. Retinal and visual pathway function was assessed by simultaneously recording pattern reversal visual evoked potentials (PRVEPs) Linear regression analyses were adopted to establish the correlation between average RNFL thickness and PRVEP parameters. Average Retinal Nerve fiber layer thickness were taken. PRVEP, P100 latency and N75-P100 amplitude were also measured. In POAG eyes, we found a significant ( P< 0.01) reduction in average RNFL thickness with respect to the values observed in control eyes. PRVEP parameters showed a significant ( p< 0.01) delay in p100 latency and reduced N75 p100 amplitude. Positive correlations between average RNFL values and PRVEP parameters were found. There is a positive correlation between PRVEP changes and average RNFL thickness, in POAG patients.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"124 1","pages":"5394-5398"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86575958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A novel method to achieve high yield of total RNA from zebrafish for expression studies 一种从斑马鱼中获得高产总RNA用于表达研究的新方法

International Journal of Bioassays Pub Date : 2017-05-08 DOI: 10.21746/IJBIO.2017.05.004

Saurabh Shukla, R. Jhamtani, M. S. Dahiya, R. Agarwal

引用次数: 7

A study on the antifungal susceptibility pattern of dermatophytes isolated in a tertiary care hospital 某三级医院分离的皮癣菌抗真菌药敏模式的研究

International Journal of Bioassays Pub Date : 2017-05-06 DOI: 10.21746/IJBIO.2017.05.003

Vanathi Sabtharishi, R. Katragadda, T. Ravinder

{"title":"A study on the antifungal susceptibility pattern of dermatophytes isolated in a tertiary care hospital","authors":"Vanathi Sabtharishi, R. Katragadda, T. Ravinder","doi":"10.21746/IJBIO.2017.05.003","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.05.003","url":null,"abstract":"Recent years, due to increased usage of antifungal treatment worldwide, there is an increased chance of rising resistance among antifungal drugs too. Dermatophytic infections causes’ superficial mycosis and it affects skin, hair and nail. These infections are more common and antifungal drugs are used everywhere to treat those common infections. To conduct a study by determining the antifungal susceptibility pattern in dermatophytic isolates from patients attending dermatology OPD in a tertiary care hospital. A total of 217 samples like hair, nail and skin scrapings were obtained and isolation of dermatophytes was done. Antifungal susceptibility testing for dermatophytes was performed by micro broth dilution method. Antifungal drugs tested were Griseofulvin, Fluconazole, Itraconazole and Ketoconazole. Minimum inhibitory concentration for each drug for fungal isolates was tested and results studied. Fluconazole showed a higher MIC values in the range of 1-8µg/ml. Itraconazole showed the lowest MIC values by micro broth dilution method. Since there is limitation of standard guidelines and protocol, meticulous research must be conducted on effect of antifungals and derive at universally implementable guidelines.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"49 1","pages":"5379-5382"},"PeriodicalIF":0.0,"publicationDate":"2017-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78630454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Biofertilizer effect of free living nitrogen fixing bacterium on the growth and water quality properties of Penaeid prawn culture system 游离固氮菌对对虾养殖系统生长和水质特性的生物肥料效应

International Journal of Bioassays Pub Date : 2017-05-06 DOI: 10.21746/IJBIO.2017.05.001

V. K. Raju, K. Sreeramulu

{"title":"Biofertilizer effect of free living nitrogen fixing bacterium on the growth and water quality properties of Penaeid prawn culture system","authors":"V. K. Raju, K. Sreeramulu","doi":"10.21746/IJBIO.2017.05.001","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.05.001","url":null,"abstract":"The role of phytoplankton is significant in improving the yield of prawn culture. The growth of phytoplankton will be effected various factors viz. dissolved oxygen, production of natural food to shrimp larvae in control of ammonia. The growth of phytoplankton can be enhanced by application of nitrogeneous fertilizers to improve water quality and growth performance of Penaeus indicus. The present research work is focussed on a free-living nitrogen fixing bacterium Azotobacter chroococcum supplemented by nitrogeneous fertilizers to increase the content of proteins, carbohydrates and wet weight of P. indicus. Ten animals of juveniles of P. indicus were collected and grown in controlled conditions for fifteen days. The liquid suspension of free living nitrogen fixing bacteria A. chroococcum has been inoculated for experimental treatment purpose. The inoculum has been introduced to four treatments and for findings of water quality analysis and phytoplankton growth analysis by Strickland and Parsons method and biochemical analysis by Raymont et al., method . The research findings revealed that A. chroococcum supplemented with fertilizers will affect the growth of phytoplankton which is vital in prawn culture and enhance the growth of hormones and dry weight for improvement of the yield.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"29 1","pages":"5369-5372"},"PeriodicalIF":0.0,"publicationDate":"2017-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79349302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virulence factors associated with organisms causing asymptomatic bacteriuria in pregnant females attending a tertiary care hospital 在三级保健医院就诊的孕妇中，与引起无症状细菌尿的生物体相关的毒力因子

International Journal of Bioassays Pub Date : 2017-05-06 DOI: 10.21746/IJBIO.2017.05.002

P. Ponnusamy, R. Katragadda, T. Ravinder

{"title":"Virulence factors associated with organisms causing asymptomatic bacteriuria in pregnant females attending a tertiary care hospital","authors":"P. Ponnusamy, R. Katragadda, T. Ravinder","doi":"10.21746/IJBIO.2017.05.002","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.05.002","url":null,"abstract":"Asymptomatic bacteriuria (ASB), most common during pregnancy is endangering as it may lead to maternal and fetal complications. Various organisms causing ASB combats the host defense mechanisms through virulence factors exhibited by them. In order to understand the pathogenesis and sequelae of infections, virulence factors like hemolysin production, gelatinase production, haemagglutination, biofilm production and many more should be identified. Hence, we aimed at studying the distribution of virulence factors among each organism causing asymptomatic bacteriuria in pregnant females attending a tertiary care hospital. Materials and Methods: This cross-sectional study was conducted in Department of Microbiology over a period of one year and six months (January 2014 to June 2015) at a tertiary care teaching hospital. A total of 1000 urine samples were included in study taken from pregnant women with asymptomatic bacteriuria. Isolation, identification of organisms was done according to standard microbiological techniques and virulence factors for individual organisms by phenotypic method were tested. Results: Out of 1000 samples screened for ASB, organisms were isolated in following frequency distribution: Escherichia coli, the commonest 54/118 (45.76%) , Klebsiella pneumoniae 21/118 (17.80%) , Staphylococcus aureus 19/118 (16.10%) , Staphylococcus saprophyticus 10/118 (8.45%), Enterococcus faecalis 9/118 (7.63%), Pseudomonas aeruginosa 3/118 (2.54%) and Proteus mirabilis 2/118 (1.69%). Virulence factors for individual organisms and biofilm detection for all organisms were done. Conclusion: Multifactorial mechanisms determine the pathogenicity of an organism and it needs to be explored by analyzing each virulence factor and mechanism of invasion in combating the host defense systems. Hence analyzing the phenotypic expression of each virulence factor helps in better understanding about the complications of ASB.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"64 5","pages":"5373-5378"},"PeriodicalIF":0.0,"publicationDate":"2017-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72634150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and phylogenetic analysis of cyclopoid copepod Mesocyclops leuckarti using mtCOI 利用mtCOI对拟环类桡足动物Mesocyclops leuckarti的分子特征及系统发育分析

International Journal of Bioassays Pub Date : 2017-04-08 DOI: 10.21746/IJBIO.2017.04.008

Gomathi Jeyam, Ramanibai R*

{"title":"Molecular characterization and phylogenetic analysis of cyclopoid copepod Mesocyclops leuckarti using mtCOI","authors":"Gomathi Jeyam, Ramanibai R*","doi":"10.21746/IJBIO.2017.04.008","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.04.008","url":null,"abstract":"Copepods are the most abundant metazoan zooplankton amongst multicellular animals. The present study was performed to establish the sequence variation of partial mitochondrial Cytochrome c Oxidase I gene (COI) from Mesocyclops leuckarti collected from the Retteri Lake, Chennai in order to identify and describe their genetic divergence along with the phylogenetic relatedness with other species. DNA of individual M. leuckarti was extracted and the partial mitochondrial COI gene was successfully amplified using the universal primers LCO-1490 and HCO-2198. A 576bp partial mitochondrial COI gene sequence was obtained. Analysis of partial COI sequences of M. leuckarti exposed 93% similarity amongst all the individual of copepods selected from Genbank. The obtained COI sequences of Cyclopoid copepod was confirmed with BLAST analysis. Phylogenetic analysis of M. leuckarti along with selected out groups from different taxa level further supports the clarity and maintained the authentic of taxonomy up to the subclass level: Copepoda. The results showed that, the COI barcoding of cyclopoid copepod species could be distinguished from the others very clearly. Thus, it strongly indicated that COI may be a useful construction of a comprehensive DNA barcode database for copepods inhabiting the freshwater bodies in Chennai.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"47 1","pages":"5365-5368"},"PeriodicalIF":0.0,"publicationDate":"2017-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81042405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0