International Journal of Bioassays最新文献

{"title":"Simultaneous assay of Mupirocin and Metronidazole in formulations using Reverse Phase-High Performance Liquid Chromatography","authors":"S. P, K. Rambabu","doi":"10.21746/IJBIO.2016.12.008","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.12.008","url":null,"abstract":"A new reverse phase-high performance liquid chromatographic method for the assay of mupirocin and metronidazole in formulation has been developed and validated as per ICH guidelines. The present study was carried on Water’s X-bridge C-18 column (4.6 x150mm, 5μ particle size) with mobile phase containing a mixture phosphate buffer (pH 2.5) and acetonitrile in the ratio of 70:30, %v/v at a flow rate of 1.0ml/min with UV detection at 220nm in ambient column temperature. The retention times for mupirocin and metronidazole were found to be 2.153 and 3.157 min respectively with linearity in the concentration range of 20-60μg/mL for mupirocin and 10-30μg/mL for metronidazole respectively. The developed reverse phase-high performance liquid chromatographic method was found to be best suitable for pharmacokinetic studies of these mentioned drugs in formulations.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"23 1","pages":"5151-5155"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83704917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular biomarkers of high altitude adaptation: Selection aid for livestock breeding","authors":"Arup Giri, V. Bharti, Sahil Kalia, B. Kumar","doi":"10.21746/IJBIO.2016.12.007","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.12.007","url":null,"abstract":"The efficiency of oxygen usage and energy metabolism is important for high-altitude adaptation and optimum performance of animals. With regards to adaptation and productivity of high yielder animals, high-altitude stress (hypobaric-hypoxia, cold stress) is a primary concern at high altitude. The cardiovascular system is an essential link in the transport of oxygen from the air to the mitochondria, and it, therefore, has an important role in acclimatisation and adaptation to the oxygen depleted environment of high altitude. These pathophysiological changes belong to the morphological, functional, and component of the cardiovascular system which can be detected as the biomarker whenever changes are found in this system. These cardiovascular biomarkers are structural, haematological, biochemical, molecular, and genetic. This lecture has reviewed the different types of cardiovascular biomarkers which are important in high altitude adaptation and therefore may be helpful in selection of adapted animals for future breeding and rearing at high altitude. Some structural biomarkers are hypertrophy of auricle and ventricle, pulmonary arterial pressure, hypertension, myocardial contractibility, hydrothorax, ascites, etc., whereas hematocrit level, blood viscosity, plateletatic, is haematological biomarkers. Cardiac troponin-I and troponin-T, brain natriuretic peptide (BNP), inflammatory markers, rennin, etc. are biochemical biomarkers. Details of these biomarkers and other related markers will be discussed in present lecture. These biomarkers concentration or levels are indicators of physiological state genetic make-up of animals of the respective environmental condition. Hence, these biomarkers along with performance and physical traits may be considered for selection high altitude adapted animals for breeding and rearing.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"139 1","pages":"5146-5150"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76798477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on antibacterial potential and phytochemical screening of different extract of Achyranthes aspera","authors":"Shyamji Shukla, Priyanka Soni, Harish K. Kewat","doi":"10.21746/IJBIO.2016.12.005","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.12.005","url":null,"abstract":"There is an alarming increase in the problem of resistance towards antibiotics amongst most of the pathogenic bacterial strains in recent years. This has drawn the attention of researchers around the world to search for novel and eco-friendly antibacterial compounds. Several biological sources have been explored in this respect but medicinal plants have taken a centre stage out of all. Plants have been known as a reservoir of number of bioactive compounds specially the antibacterial ones since time immemorial. Therefore, the present investigation was undertaken to analyze the antibacterial potential of the medicinal plant Achyranthes aspera. This study revealed that highest antibacterial activity was observed in the methanolic extract of stem against almost all test Bacteria. It showed maximum activity against E.coli (30 mm), followed by S. aureus (28 mm), Enterococcus sp.(25mm), Salmonella typhi ( 20 mm) and least activity was recorded in same extract against K.pneumoniae (6 mm). Four phytochemicals were screened in various solvent extracts. They are alkaloid, flavonoids, saponins and tannins.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"22 5","pages":"5138-5142"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91484969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of potential salt tolerant bacteria for soybean growth promotion","authors":"Anukool Vaishnav, S. Kumari, Shekhar Jain, D. Choudhary, K. Sharma","doi":"10.21746/IJBIO.2016.12.001","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.12.001","url":null,"abstract":"Salinity is a major limiting factor for soybean crop productivity. To enhance the tolerance of soybean against salt stress, it is essential to understand the diversity of microbiota harboured by soybean rhizosphere. Earlier studies have demonstrated that local adaptation of plants to habitat imposed stresses is driven by their closely associated microbes. The present study aimed to isolation and characterization of salt tolerant rhizobacteria with respect to their functional plant growth promotion ability. A total of 43 bacterial isolates were recovered from soybean rhizospheric soil grown in Bundi district, Rajasthan, India. Out of them, one bacterial isolate strain AU was found to tolerate 10% NaCl stress and significantly enhanced soybean seedlings growth under 100mM NaCl condition. Molecular phylogenetic analysis placed this isolate closely to Pseudomonas simiae OLi T with 99.93% similarity. Molecular characterization of functional genes revealed that AU bacterium possessed genes like IaaM (IAA production), g6pd (Pi-solubilization) and sid (siderophore production). Here, we show that soybean rhizosphere possessed a salt tolerant plant growth promoting bacterium strain AU, which may have impacts on alleviation and tolerance of salt stress in legume plants.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"52 1","pages":"5118-5123"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77255344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taurine improves lambda cyhalothrin induced biochemical alterations in Wistar rat liver","authors":"R. Ghosh, Tuhina Das, A. Paramanik, S. Choudhury","doi":"10.21746/IJBIO.2016.11.0013","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.0013","url":null,"abstract":"Taurine is a major intracellular free β-amino acid, which can protect the body against toxicity. Lambda-cyhalothrin, a third-generation type II pyrethroid. is used predominantly in agriculture production and animal husbandry. The aim of the present study was to investigate lambda cyhalothrin-induced biochemical changes in rat liver and to search out the possible role of taurine for the attenuation of hepatotoxic biomarkers.  Male rats were randomly divided into six groups and lambda cyhalothrin was orally administered at two dose levels (10.83mg/body wt., 15.17mg/body wt.) alone and in combination with taurine pretreatment (50mg/kg body wt) for 14 consecutive days. A significant change in blood glucose level with a marked decline in glycogen content were indicated the hepatic dysfunction in lambda cyhalothrin treated rats. This was also confirmed by the altered activities of serum hepatic biomarker enzymes and lipid profiles in LCT intoxicated rats. Pre-treatment of taurine mitigated the abnormalities. These findings pointed out the toxic effect of lambda cyhalothrin in rat liver and also revealed the protective action of taurine against this pyrethroid.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"33 1","pages":"5059-5065"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85397560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Major tea processing practices in India","authors":"Satyajit Sarkar, Anurag Chowdhury, Sanjay Das, Bhaskar Chakraborty, P. Mandal, M. Chowdhury","doi":"10.21746/IJBIO.2016.11.0015","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.0015","url":null,"abstract":"Tea is world’s most popular beverages and is the economical backbones of various tea producing countries like India, China, Sri Lanka, Kenya etc. Indian tea is very much famous in the world, especially Darjeeling tea which is famous for its unique flavour and taste. Among the various types of tea, Orthodox tea is highly demandable among the various types of tea due to its quality. Present article focused on different types of major tea processing in India. In India, mostly three types of tea processing are practices, among them, CTC tea processing is more common followed by Green tea and Orthodox tea. In this paper, step wise details manufacturing process are presented. All the data are extracted during the extensive survey in different world famous tea gardens of Darjeeling hills, terai and duars of Northern West Bengal.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"16 1","pages":"5071-5083"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79192383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between pamphlets and SMS on patterns of breakfast and between meal based on the health belief model among girl students of high schools in Sheiban city during academic year 2015-2016","authors":"S. Moradi, K. Karami, G. S. Nejad, A. Malehi","doi":"10.21746/IJBIO.2016.11.003","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.003","url":null,"abstract":"Nutrition has a direct role in creating, maintaining and improving of health. Breakfast is considered as one of the main meals and snacks as a major part of energy and nutrients supplier for children. This study aim to comparison between pamphlets and SMS instruction on breakfast and snack food consumption pattern based on the Health Belief Model among girls in high schools of Sheiban City. This intervention study involved three groups that were conducted by the two intervention groups and a control group among 300 female high school students. To determine the samples, three schools of girls and from each school 100 students were selected using random sampling method. The tools of data collection was a questionnaire which validated by a panel of experts and test-retest reliability and Cronbach's alpha calculation. Educational intervention was distribution of pamphlets and texting to the pamphlet and SMS groups, respectively. The statistical analysis of variance and covariance of collected data were performed by SPSS software version 22. The results were significant at level of P≤0.05. Comparing the two control and tested groups showed that mean score of Health Belief Model (susceptibility, severity, benefits, barriers, self-efficacy and cues to action) as well as breakfast and snack food consumption behavior scores of students significantly increased after the educational intervention in the intervention group compared to the control, but the education effects in both groups were similar. Education based on Health Belief Model by SMS and pamphlets was effective on breakfast and snack consumption behavior. There was no significant difference between educational effects of two methods .","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"12 1","pages":"5004-5010"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81854855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cheliped abnormality and embryonic development of commercially important portunid crab, Portunus sanguinolentus (L.)","authors":"B. Bhat, M. A. Parray, Sartaj Ahmad Allayie, S. Ravichandran, C. Elanchezhiyan","doi":"10.21746/IJBIO.2016.11.0018","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.0018","url":null,"abstract":"In the present study, an attempt was made to study the cheliped abnormalty of commercially important crab, Portunus sanguinolentus . The newly spawned eggs were round and golden yellow in colour. The undeveloped and mass of undifferentiated cells were also found in the berry. The yolk granules were denser. The cleavage and gastrulation were not clear. The diameter of the freshly laid egg was 0.34mm. The multicell eggs were round, deep yellow or yellowish orange in colour. The space between the egg wall and the inner developing embryo was visible. The diameter of the egg was 0.41mm. The eye stage eggs were round and orange in colour. The yolk granules were not denser. The egg diameter was 0.48mm. The pigment stage eggs were brown in colour. The egg diameter was 0.57 mm. The diameter of the egg was 0.64 mm. In prehatching stage the rate of heartbeat was increased and the chromatophores were also found increased throughout the body. The diameter of the egg was 0.73mm. Hence in the present study both the embryonic as well abnormality of cheliped was under taken in to the consideration.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"56 1","pages":"5097-5104"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80210934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical evaluation of leaf extracts of Naringi crenulata (roxb.) Nicolson","authors":"Sartaj Ahmad Allayie, M. A. Parray, B. Bhat, S. Hemalatha","doi":"10.21746/IJBIO.2016.11.0020","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.0020","url":null,"abstract":"The use of traditional medicines holds a great promise as an easily available source as effective medicinal agents to cure a wide range of ailments among the people particularly in tropical developing countries like India. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of N. crenulata leaf extracts. The extractive values of aqueous, acetone and chloroform extracts were found to be 11.34, 4.24 and 6.06 respectively. Qualitative phytochemical analysis of these three solvent extracts confirm the presence of Alkaloids, Saponins, Flavonoids and Phenolic compounds in all the three extracts; however, these phytochemicals were more significant in aqueous extract. Quantitative analysis was carried out using TLC method by different solvent system. Amongst various solvent systems, Butanol: acetic acid: water (9: 0.9: 0.1 v/v/v) shows maximum resolution and number of spots produced at long UV (365 nm) and under iodine vapours. The TLC chromatograms constituted different coloured phytochemical compounds with different Rf values. It can be conveniently used to evaluate the quality of different area samples. This indicates that the leaves can be useful for treating different diseases because the therapeutic activity of a plant is due to the presence of particular class of compounds and thus can serve as potential sources of useful drugs in future .","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"10 1","pages":"5110-5117"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88074964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Alu I polymorphism in estrogen receptor beta gene with adverse pregnancy outcome in HEV infection","authors":"S. Singh, V. K. Karra, M. Daga, A. Kumar, S. Husain, S. Choudhary, R. Hazam, Pk Gumma, S. Polipalli, P. Kar","doi":"10.21746/IJBIO.2016.11.006","DOIUrl":"https://doi.org/10.21746/IJBIO.2016.11.006","url":null,"abstract":"Hepatitis E has both a high incidence and severe course in pregnant women in some geographic regions of HEV (Hepatitis E virus) endemic countries. Intrauterine fetal death, preterm delivery, and perinatal mortality are reported to be higher in pregnant women with HEV infection. Alteration in the steroid hormone levels show high incidence of FHF (Fulminant Hepatic Failure) with high mortality in hepatitis E during pregnancy. The present study is designed to look for the association between ESR (Estrogen Receptor)-beta gene polymorphism for Alu I restriction site and pregnancy outcome. The study group comprised of 142 pregnant women with HEV infection in third trimester, 103 AVH (Acute Viral Hepatitis) cases and 39 ALF (Acute Liver Failure) cases. The control group comprised of 142, age and gestation matched healthy pregnant women with no obstetrics and medical complications. The inclusion criteria for the study group are pregnant women in third trimester with positivity to HEV IgM and/or HEV RNA in the age group of 18-40 years. Genomic DNA is extracted from peripheral blood leukocytes using DNA extraction kit according to the manufacturer’s instructions. The polymorphism study is done by using ESR2 specific primers and its genotype is determined by Alu I restriction enzyme. The occurrence of variant A allele for AluI restriction site is significantly higher in mothers with HEV infection who had preterm (25%) than full term delivery (10%) with OR 2.989 (95% CI = 1.265-8.084, p<0.05) and low birth weight (26.6%) than average birth weight (6.3%) babies with OR 5.399 (95% CI= 2.01-18.26, p<0.05) in pregnant women with HEV infection. The occurrence of variant A allele of ESR beta for AluI restriction site is significantly higher in mother of low birth weight babies (23%) than average birth weight babies (5.5%) in AVH group with OR 5.056 (95% CI= 1.634-21.57, p<0.05) and preterm (40.5%) than full term (0%) delivery in ALF group (p= 0.04). The higher occurrence of variant A allele for AluI restriction site of ESR-beta gene polymorphism is found to be associated with preterm delivery and low birth weight in pregnant women with HEV infection, preterm delivery in ALF group and low birth weight babies in AVH group.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"14 1","pages":"5021-5024"},"PeriodicalIF":0.0,"publicationDate":"2016-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75341665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}