International Journal of Bioassays最新文献

{"title":"Antibacterial effect of medicinal rue extracts on the infectious mouth in Al – Najaf community. An In-vivo study","authors":"S. A. Ibrahim","doi":"10.21746/IJBIO.2018.7.1.1","DOIUrl":"https://doi.org/10.21746/IJBIO.2018.7.1.1","url":null,"abstract":"Aim : Clinical evaluation of antibacterial and antimicrobial properties for herbal extracts plants as (rue) to have been investigated in order to suggest them as potential mouth rinse to overcome the microbial effect in the mouth of patients.   Materials and Methods:  Assessing the opposite effect of the extract of this plant (rue) on gingivitis. Using precise dilution in distilled water only without the addition of any other material of this extract a certain concentration of 20%. I've been watching influence through the state of the gum tissue surrounding the teeth clinical examination before and after use of this extract for a period of 10 days for a group of patients totaling 32 patients (25 females and 7 males), age (10-80 years). A swabs were taken of the bacteria from the mouth of the patients were cultured before treatment to observe bacterial growth and swab after the treatment to note the death of bacteria. Results: The clinical use of direct observation of signs and symptoms after the examination. Rue have a good function on inflammation of the gums and tissues surrounding the teeth after using the vegetable extract of rue material through actual viewing and examination of periodontal pockets. Also after taking swabs and cultured them.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"89 1","pages":"5464-5467"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85833832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival of bacterial and fungal spores in the expose-r2 experiment","authors":"N. Novikova, S. Poddubko, E. Deshevaya, V. Sychev","doi":"10.21746/IJBIO.2018.7.4.1","DOIUrl":"https://doi.org/10.21746/IJBIO.2018.7.4.1","url":null,"abstract":"The primary goal of the Biodiversity experiment, which was part of the Expose-R2 study, was to assess the effects of a prolonged exposure to the space environment on the survival of dormant microorganisms. To do this, units containing samples of bacterial and fungal spores were mounted on a support platform attached to the exterior wall of the Russian Zvezda Module of the International Space Station (ISS). After a 15-mon exposure to outer space the samples were returned to Earth. The experiment demonstrated that microbial spores had the capability to survive and remain viable after the exposure to outer space for the time period comparable to the Earth-Mars-Earth mission duration. This finding is not only of theoretical importance but also of practical significance as related to the development of adequate measures of planetary protection for deep space missions.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"30 1","pages":"5616-5622"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86464028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycopene and ketotifen potentiates the anti-hyperalgesic effect of gabapentin in PSNL-induced neuropathic pain in wistar rats","authors":"N. Tyagi","doi":"10.21746/IJBIO.2018.7.1.2","DOIUrl":"https://doi.org/10.21746/IJBIO.2018.7.1.2","url":null,"abstract":"The present study is designed to investigate the involvement of antioxidant mechanism and role of mast cells in pathophysiology of neuropathic pain induced by partial sciatic nerve ligation. The effect was evaluated by assessing various behavioural parameters (thermal hyperalgesia, cold hyperalgesia), biochemical parameters (lipid peroxidation, reduced glutathione, superoxide dismutase and catalase). Partial Sciatic nerve ligation (PSNL) significantly caused thermal hyperalgesia, cold hyperalgesia and oxidative damage as compared to normal and sham and group. Upon daily administration of combination of lycopene (50 mg/kg), ketotifen (10mg/kg) and gabapentin (100 mg/kg) considerably reversed hyperalgesia, cold hyperalgesia and also attenuated oxidative stress when compared to PSNL group. The results indicated that combination lycopene, ketotifen and gabapentin exhibited a potentiating effect in neuropathic pain by inhibiting degranulation of mast cells and also by reducing oxidative stress.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"2 1","pages":"5568-5573"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78323430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of variations in the shape of coronoid process in dry adulthuman mandibles","authors":"Meril Ann Soman, Rani Nallathamby","doi":"10.21746/IJBIO.2018.7.3.2","DOIUrl":"https://doi.org/10.21746/IJBIO.2018.7.3.2","url":null,"abstract":"Introduction: The coronoid process is one of the bony processes of the ramus of the mandible. It is a thin, triangular eminence, which is flattened from side to side. It varies in shape and size. The shape of coronoid process acts as an evolutionary marker. It is useful in anthropological and forensic studies. This study was undertaken to note the form of presentations of coronoid process and their incidence in the dry adult human mandibles. Method: The present study was conducted in the Department of Anatomy, Yenepoya Medical College, Mangalore. A total of hundred adult human mandibles were included in the study which consisted of 62 male and 38 female mandibles. The shapes of the coronoid processes were studied bilaterally in all the mandibles and the incidence of the shapes were analyzed statistically in both male and female mandibles. Results: Three types of variations in the shape were evident. Triangular, hook shaped and rounded. Hook shaped coronoid process was found in 90 (45%) sides, round shape in 64(32%) sides and triangular in 46 (23%) sides. Conclusion: Knowledge regarding the morphological shapes of the Coronoid process is useful for the maxillofacial surgeons during reconstructive surgeries and can be used as a donor site for sinus augmentation. It is also useful in anthropological studies and in forensic dentistry.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"79 1","pages":"5612-5615"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80002274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on population fluctuation of cucurbit fruit flies, Bactrocera cucurbitae associated with cucurbit crops","authors":"Sohrab, C. Prasad, W. Hasan","doi":"10.21746/ijbio.2018.7.6.1","DOIUrl":"https://doi.org/10.21746/ijbio.2018.7.6.1","url":null,"abstract":"The population of cucurbit fruit flies, Bactrocera cucurbitae of bitter gourd was observed vary much changeability at Block Daurala, District Meerut. Decreasing temperature negative effect was observed in cucurbits fruit flies population at District Meerut but positive effect of temperature was observed in cucurbit fruit population at District Saharanpur. Fruit flies population of cucurbits fruit increased with the increase of relative humidity, rainfall and decreased temperature while negative relationship was observed with maximum correlation in cucurbits in zaid and Kharif. Moderate to high negative correlation coefficients were observed between maximum temperature and population fluctuation of cucurbit fruit flies in cucurbits at both places. The significant positive and negative correlation coefficients were present between relative humidity, rainfall and temperature and percentage of fruit flies population","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"74 1","pages":"52-58"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80793860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Toxicity Induced by Different Domestic and Industrial Wastes in Nile Tilapia (Oreochromis niloticus)","authors":"N. Rizk, M. Goher, Ayman S. Eldourghamy, W. Aboulthana, Y. Hagag","doi":"10.21746/IJBIO.2018.7.5.3","DOIUrl":"https://doi.org/10.21746/IJBIO.2018.7.5.3","url":null,"abstract":"The River Nile represents the principle freshwater resource. It represents the major source for the potable water which is susceptible to be polluted by different metals. Heavy metals are the most common pollutants in aquatic environment. The present study aimed to evaluate the adverse effect of heavy metals on in liver and kidney tissues of Nile Tilapia (Oreochromis niloticus) that were collected from El-Kanater El-Khayria and two discharge points (El-Rahawy drain and Kafr El-Zayat industrial area) at Rosetta branch, River Nile, Egypt. During the present study, it was found that concentration of Lead (Pb), Cadmium (Cd), Iron (Fe) and Zinc (Zn) elevated significantly (P<0.05) in livers of fishes living in Rahawy and Kafr El-Zayat with respect to liver of fishes living in water of kanater. No differences observed significantly in concentration of Nickle (Ni), Chromium (Cr), Cobalt (Co) and Copper (Cu). On the other hand, it was noticed that concentration of Pb, Cd, Ni, Fe and Zn increased significantly (P<0.05) in kidney of fishes living in Rahawy and Kafr El-Zayat with respect to kidney of fishes living in water of kanater. There were no significant differences in concentration of Co and Cu. Bioaccumulation of the various heavy metals in liver and kidney tissues of fishes caused different mutagenicity in the native protein and isoenzymes detected electrophoretically. These alterations were represented qualitatively by hiding of the normal bands or appearance of abnormal bands with different relative mobilities and quantities. Moreover, these abnormalities might occur quantitatively by changing quantities of the qualitatively normal bands. The study concluded that presence of the heavy metals with higher concentration could be toxic and affect fishers and other aquatic organisms and this is worrisome in view of the health implications for the population that need fish resources to cover the food requirements.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"1 1","pages":"5645-5651"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91116447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Serum Metalloprotinase-9 (MMP-9) in Iraqi Men with urinary tract infection","authors":"H. Abbas","doi":"10.21746/IJBIO.2017.6.12.1","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.6.12.1","url":null,"abstract":"Objective: The inflammation of the urinary tract and its various causes is one of the important diseases, which neglect and non-treatment lead to many amplifications, therefore, many countries including the United States of America has given urinary tract infection ″ UTI ″ a great interest. Since the disease is sometimes without obvious symptoms and because of research lacking that are using MMP-9 . The aim of this work is to study the alterations in the Metalloprotinase-9 (MMP-9), and its role in pathogenicity of urinary tract infections ″UTI″ in serum of men who are initially diagnosed with this disease. Methods: This study was applied in educational Alsader hospital in Najaf city by using Enzyme Linked Immuno Sorbent Assay (ELISA) on serum taken from 42 men infected with UTI, and other 38 healthy considered as a control group to detect MMP-9. Data analysis: SPSS computerizing system has been used for statistically analysis of data . Results: The results revealed a highly significant (P<0. 05) level of ″MMP-9″in serum of patients in comparison with the control group. The result also demonstrated the strong relation between MMP-9 and other parameters such as age and smoking. Conclusion: It was concluded from the results of the present study thatMetalloprotinase-9″MMP-9″ was good prognostic biomarker for ″UTI″ in men, and these results significantly correlate with the early stages of disease. Recommendation: 1-It may be recommended that using MMP-9 to predict and diagnosis the urinary tract infection. 2-It may be also recommended to measure MMP-9 in women to establish its role in this disease","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"460 1","pages":"5558-5561"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84287884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report on Subacute Combined Degeneration of spinal cord in children – A rare neurological manifestation in Vitamin B12 deficiencies","authors":"T. M. Alexander, V. Pande, S. Agarkhedkar, D. Upase","doi":"10.21746/IJBIO.2017.6.12.2","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.6.12.2","url":null,"abstract":"Megaloblastic anemia is a common feature between 6 months – 2 years and rarely occurs after 5 years of age, especially in a child consuming non-vegetarian diet. B12 deficiency may occur after 5 years of age because of chronic diarrhea, malabsorption syndrome, or intestinal surgical causes. Pernicious anemia causes B12 deficiency, but nutritional B12 deficiency with subacute combined degeneration causing ataxia is rare.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"21 1","pages":"5562-5563"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76753941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on influence of education and occupation on family planning practices in rural Shamirpet, R.R. District, T.S., India.","authors":"L. Vijayasree","doi":"10.21746/IJBIO.2017.6.11.1","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.6.11.1","url":null,"abstract":"Family planning is an important pillar for safe motherhood and good reproductive health and is influenced by number of socio-demographic factors. Since fertility in India is primarily marital this study was planned among married women. A community based cross-sectional study was conducted among 402 women of reproductive age group. Details regarding various socio-demographic factors with special emphasis on education and occupation of husband and wife and their socio-economic status and its relation to contraceptive usage were studied. Prevalence of contraception was found to be 61.7%. Contraceptive usage had a significantly positive relation with literacy status of women, socio economic status of the couple. On the other hand husband’s educational status, occupation of women, husband’s occupation did not have any relation with contraceptive usage.","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"32 1","pages":"5525-5529"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76897002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of standard permissible levels of Lead (Pb) for potable waters on fish innate immune response and health compared with Pb levels found in natural waterbodies","authors":"Grayson Orvile Halley","doi":"10.21746/IJBIO.2017.6.11.2","DOIUrl":"https://doi.org/10.21746/IJBIO.2017.6.11.2","url":null,"abstract":"Among the list of pollutants, the heavy metals group is attracting the focus of Aquaculturist, Environmentalist and Fisherfolks alike, since they not only affect aquatic organisms but also have the potential to ultimately affect human beings. The deleterious effects of heavy metals such as lead (Pb) on aquatic ecosystems necessitate continuous monitoring of its accumulation in key species since it affords an indication of its impact on organism’s health. In natural waters, the total Pb concentrations range between 0.05 and 10.0 mg/l (Galvin 1996). While the standard recommended a limit for water for consumption range between 0.01 and 0.05 mg/L Pb. In the current study, we exposed juvenile tilapias ( Oreochromis niloticus) to waterborne Pb in five concentrations 0 (control), 0.01, 0.05, 0.25 and 1.25 mg/L Pb, over six weeks period. The present study focused on the bioaccumulation of low to moderate concentrations of Pb while simultaneously assessing the effects of waterborne Pb on O. niloticus feed intake, growth performances, blood plasma  Lysozyme, Immunoglobulin M (IgM), Complement 3 (C3) and Cortisol levels. Results from the present study showed that among the Pb exposed groups a trend emerged, which indicated a high bioaccumulation rate in fish exposed to the lower (0.01, 0.05 and 0.25 mg/L) Pb concentrations. Fish exposed to 0.01 mg/l Pb showed highest accumulation rates, after 2 weeks of exposure, they were able to accumulate muscle Pb level that was equal to the concentration of Pb to which they were exposed. Meanwhile, data showed that fish exposed to 0.05 and 0.25 mg/L waterborne Pb recorded mean muscle Pb levels equal to or above the concentration of Pb to which they were exposed in the 4th week of exposure time. Furthermore, the mean muscle Pb recorded in fish after the 6th week of exposure to 0.01 mg/L waterborne Pb was 6 times above the level of the water concentration, while fish exposed to 0.05 mg/L, 0.25 mg/L and 1.25 mg/L waterborne Pb recorded mean muscle Pb after the 6 th week that was 5, 3 and 2 times above the concentration of Pb in their respective water environment. Compared with the control group no significant difference in C3 activities were observed in fish exposed to 0.01 mg/L, 0.05 mg/L, and 0.25 mg/L Pb; however, as the dose of Pb increased to 1.25 mg/L significant decrease in C3 activity was observed after 4 weeks of exposure when compared with the control group. Additionally, no significant difference in IgM activities was observed in the 0.01 mg/L, 0.05 mg/L and 0.25 mg/L Pb treated groups of fish when compared with the control respectively. However, as the dose of Pb increased to 1.25 mg/L significant decrease in IgM activity was observed after 2 weeks of exposure compared with the control group. Similarly, as the dose of Pb was increased to 1.25 mg/L significant decrease in lysozyme activity was observed after 2 weeks of exposure compared to the control group. While Pb dose 0.25 mg/L after 4 weeks of exposure sh","PeriodicalId":13765,"journal":{"name":"International Journal of Bioassays","volume":"50 1","pages":"5543-5557"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74022374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}