International immunology最新文献_第9页

Lung group 2 innate lymphoid cells differentially depend on local IL-7 for their distribution, activation, and maintenance in innate and adaptive immunity-mediated airway inflammation. 肺2组先天性淋巴细胞在先天和适应性免疫介导的气道炎症中不同地依赖于局部IL-7的分布、激活和维持。

IF 4.4 4区医学

International immunology Pub Date : 2023-11-07 DOI: 10.1093/intimm/dxad029

Daichi Takami, Shinya Abe, Akihiro Shimba, Takuma Asahi, Guangwei Cui, Shizue Tani-Ichi, Takahiro Hara, Keishi Miyata, Masashi Ikutani, Kiyoshi Takatsu, Yuichi Oike, Koichi Ikuta

{"title":"Lung group 2 innate lymphoid cells differentially depend on local IL-7 for their distribution, activation, and maintenance in innate and adaptive immunity-mediated airway inflammation.","authors":"Daichi Takami, Shinya Abe, Akihiro Shimba, Takuma Asahi, Guangwei Cui, Shizue Tani-Ichi, Takahiro Hara, Keishi Miyata, Masashi Ikutani, Kiyoshi Takatsu, Yuichi Oike, Koichi Ikuta","doi":"10.1093/intimm/dxad029","DOIUrl":"10.1093/intimm/dxad029","url":null,"abstract":"Interleukin-7 (IL-7) is a cytokine critical for the development and maintenance of group 2 innate lymphoid cells (ILC2s). ILC2s are resident in peripheral tissues such as the intestine and lung. However, whether IL-7 produced in the lung plays a role in the maintenance and function of lung ILC2s during airway inflammation remains unknown. IL-7 was expressed in bronchoalveolar epithelial cells and lymphatic endothelial cells (LECs). To investigate the role of local IL-7 in lung ILC2s, we generated two types of IL-7 conditional knockout (IL-7cKO) mice: Sftpc-Cre (SPC-Cre) IL-7cKO mice specific for bronchial epithelial cells and type 2 alveolar epithelial cells and Lyve1-Cre IL-7cKO mice specific for LECs. In steady state, ILC2s were located near airway epithelia, although lung ILC2s were unchanged in the two lines of IL-7cKO mice. In papain-induced airway inflammation dependent on innate immunity, lung ILC2s localized near bronchia via CCR4 expression, and eosinophil infiltration and type 2 cytokine production were reduced in SPC-Cre IL-7cKO mice. In contrast, in house dust mite (HDM)-induced airway inflammation dependent on adaptive immunity, lung ILC2s localized near lymphatic vessels via their CCR2 expression 2 weeks after the last challenge. Furthermore, lung ILC2s were decreased in Lyve1-Cre IL-7cKO mice in the HDM-induced inflammation because of decreased cell survival and proliferation. Finally, administration of anti-IL-7 antibody attenuated papain-induced inflammation by suppressing the activation of ILC2s. Thus, this study demonstrates that IL-7 produced by bronchoalveolar epithelial cells and LECs differentially controls the activation and maintenance of lung ILC2s, where they are localized in airway inflammation.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"513-530"},"PeriodicalIF":4.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9925211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental Sjögren's syndrome. IL-27调节实验性干燥综合征中MDSCs的NLRP3炎症小体激活。

IF 4.4 4区医学

International immunology Pub Date : 2023-11-07 DOI: 10.1093/intimm/dxad037

Xiaoyu Xia, Qiqiang Long, Jie Zha, Tingting Jiang, Junqiao Guo, Bo Jiang, Xiaojing Li, Genhong Yao

{"title":"IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental Sjögren's syndrome.","authors":"Xiaoyu Xia, Qiqiang Long, Jie Zha, Tingting Jiang, Junqiao Guo, Bo Jiang, Xiaojing Li, Genhong Yao","doi":"10.1093/intimm/dxad037","DOIUrl":"10.1093/intimm/dxad037","url":null,"abstract":"Excessive NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome activation has an important function in the pathogenesis of Sjögren's syndrome (SS). Increased and dysfunctional myeloid-derived suppressor cells (MDSCs) promoted SS. However, NLRP3 inflammasome activation of MDSCs in SS and its regulated components are unclear. Splenic MDSCs were purified by immunomagnetic beads and cultured. Western blot was used to assess NLRP3 inflammasomes. Interleukin-1β (IL-1β) and IL-18 were measured using enzyme-linked immunosorbent assay. Here we showed that the NLRP3 inflammasome was activated in non-obese diabetic (NOD) mice with SS-like manifestations. We found that NLRP3 inflammasome activation was augmented in MDSCs of SS mice and NLRP3 inflammasome activation was suppressed in IL-27-deficient NOD mice. Consistent with findings of SS mice in vivo, we observed that NLRP3 inflammasome activation by adenosine triphosphate and lipopolysaccharide was remarkably intensified in MDSCs with IL-27 treatment in vitro. Collectively, our data highlighted that IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental SS.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"531-542"},"PeriodicalIF":4.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Immunogenicity evaluation of viral peptides via nonspecific interactions between anti-peptide IgYs and non-cognate peptides. 修正:通过抗肽IgYs和非同源肽之间的非特异性相互作用来评估病毒肽的免疫原性。

IF 4.4 4区医学

International immunology Pub Date : 2023-11-07 DOI: 10.1093/intimm/dxad023

引用次数: 0

Correction to: Lung group 2 innate lymphoid cells differentially depend on local IL-7 for their distribution, activation, and maintenance in innate and adaptive immunity-mediated airway inflammation. 更正：肺2组先天性淋巴细胞在先天和适应性免疫介导的气道炎症中不同地依赖于局部IL-7的分布、激活和维持。

IF 4.4 4区医学

International immunology Pub Date : 2023-11-07 DOI: 10.1093/intimm/dxad036

引用次数: 0

Mucosal-associated invariant T cells from Clostridioides difficile-infected patients exhibit a distinct proinflammatory phenotype and enhanced cytotoxic activity. 来自艰难梭菌感染患者的粘膜相关不变T细胞表现出明显的促炎表型和增强的细胞毒性活性。

IF 4.4 4区医学

International immunology Pub Date : 2023-11-07 DOI: 10.1093/intimm/dxad032

Steffen Brauns, Isabel Marquardt, Cosima Thon, Sarah Frentzel, Josefine Jakob, Jacqueline Färber, Lars Philipsen, Lothar Jänsch, Alexander Link, Dunja Bruder

{"title":"Mucosal-associated invariant T cells from Clostridioides difficile-infected patients exhibit a distinct proinflammatory phenotype and enhanced cytotoxic activity.","authors":"Steffen Brauns, Isabel Marquardt, Cosima Thon, Sarah Frentzel, Josefine Jakob, Jacqueline Färber, Lars Philipsen, Lothar Jänsch, Alexander Link, Dunja Bruder","doi":"10.1093/intimm/dxad032","DOIUrl":"10.1093/intimm/dxad032","url":null,"abstract":"Mucosal-associated invariant T (MAIT) cells are innate-like T cells mainly found in the mucosa and peripheral blood. We have recently demonstrated that Clostridioides difficile activates MAIT cells in vitro. However, their role in the pathogenesis of C. difficile infection (CDI) in human patients remains elusive to date. In this study, we performed comprehensive immunophenotyping of MAIT cells derived from CDI patients and compared their phenotype to that of patients with inflammatory bowel diseases (IBD) and healthy controls. Our study revealed that blood MAIT cells from CDI patients exhibit an interleukin 17a (IL-17a)-dominated proinflammatory phenotype and an increased readiness to synthesize the proinflammatory cytokine interferon γ (IFN-γ) following in vitro re-stimulation. Moreover, the cytotoxic activity of MAIT cells, as measured by surface CD107a and intracellular granzyme B expression, was strongly increased in CDI. Multi epitope ligand cartography (MELC) analysis of intestinal biopsies from CDI patients revealed that MAIT cells exhibit an increased production of granzyme B and increased cytotoxicity compared to the control group. Together with previously published in vitro data from our group, our findings suggest that MAIT cells are functionally involved in the immune response against C. difficile and contribute to the pathogenesis of CDI.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"543-554"},"PeriodicalIF":4.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outstanding Merit Award 2023 2023年杰出优异奖

4区医学

International immunology Pub Date : 2023-10-31 DOI: 10.1093/intimm/dxad039

引用次数: 0

Introduction: Novel Aspects of the Germinal Center Reaction Special Issue 导言:生发中心反应的新方面特刊

4区医学

International immunology Pub Date : 2023-10-20 DOI: 10.1093/intimm/dxad040

Tomohiro Kurosaki

引用次数: 0

Decreased TAX1BP1 participates in systemic lupus erythematosus by regulating monocyte/macrophage function. TAX1BP1降低通过调节单核细胞/巨噬细胞功能参与系统性红斑狼疮。

IF 4.4 4区医学

International immunology Pub Date : 2023-10-06 DOI: 10.1093/intimm/dxad027

Tian Qian, Bengang Huo, Xiaorong Deng, Xiaoli Song, Yiwei Jiang, Jurong Yang, Fei Hao

{"title":"Decreased TAX1BP1 participates in systemic lupus erythematosus by regulating monocyte/macrophage function.","authors":"Tian Qian, Bengang Huo, Xiaorong Deng, Xiaoli Song, Yiwei Jiang, Jurong Yang, Fei Hao","doi":"10.1093/intimm/dxad027","DOIUrl":"10.1093/intimm/dxad027","url":null,"abstract":"Systemic lupus erythematosus (SLE) involves disorders of innate and adaptive immune pathways. Tax1-binding protein 1 (TAX1BP1) modulates the production of antibodies in B cells and the T-cell cycle by regulating the NF-κB signaling pathway. However, the potential association of TAX1BP1 with SLE and its role in monocytes/macrophages have not been fully elucidated. In this study, we utilized whole-exome sequencing (WES) in combination with Sanger sequencing and identified 16 gene mutations, including in TAX1BP1, in an SLE family. TAX1BP1 protein expression with western blotting detection was reduced in SLE patients and correlated with disease activity negatively. Furthermore, RNA sequencing and 4D Label-Free Phosphoproteomic analysis were employed to characterize the transcriptome and phosphoproteome profiles in THP-1 and THP-1-differentiated M1 macrophages with TAX1BP1 knockdown. Silencing of TAX1BP1 in THP-1 and THP-1-differentiated M1 macrophages led to an increase in cluster of differentiation 80 (CD80) expression and differential changes in CD14 and CD16 expression, as assessed by flow cytometry. Additionally, western blot analysis showed that knockdown of TAX1BP1 led to a reduction in TRAF6 and p-p65 in THP-1-differentiated macrophages, with or without lipopolysaccharide (LPS) or tumor necrosis factor (TNF)-α stimulation. Taken together, our findings suggest that TAX1BP1 participates in SLE activity by regulating antigen presentation in monocytes and inflammatory responses in M1 macrophages.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"483-495"},"PeriodicalIF":4.4,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9828927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NOTCH4 potentiates the IL-13 induced genetic program in M2 alternative macrophages through the AP1 and IRF4-JMJD3 axis. NOTCH4通过AP1和IRF4-JMJD3轴增强M2替代巨噬细胞中IL-13诱导的遗传程序。

IF 4.4 4区医学

International immunology Pub Date : 2023-10-06 DOI: 10.1093/intimm/dxad028

Susana López-López, María José Romero de Ávila, María Julia González-Gómez, María Luisa Nueda, Victoriano Baladrón, Eva M Monsalve, José Javier García-Ramírez, María José M Díaz-Guerra

{"title":"NOTCH4 potentiates the IL-13 induced genetic program in M2 alternative macrophages through the AP1 and IRF4-JMJD3 axis.","authors":"Susana López-López, María José Romero de Ávila, María Julia González-Gómez, María Luisa Nueda, Victoriano Baladrón, Eva M Monsalve, José Javier García-Ramírez, María José M Díaz-Guerra","doi":"10.1093/intimm/dxad028","DOIUrl":"10.1093/intimm/dxad028","url":null,"abstract":"IL-13 signaling polarizes macrophages to an M2 alternatively activated phenotype, which regulates tissue repair and anti-inflammatory responses. However, an excessive activation of this pathway leads to severe pathologies, such as allergic airway inflammation and asthma. In this work, we identified NOTCH4 receptor as an important modulator of M2 macrophage activation. We show that the expression of NOTCH4 is induced by IL-13, mediated by Janus kinases and AP1 activity, probably mediated by the IL-13Rα1 and IL-13Rα2 signaling pathway. Furthermore, we demonstrate an important role for NOTCH4 signaling in the IL-13 induced gene expression program in macrophages, including various genes that contribute to pathogenesis of the airways in asthma, such as ARG1, YM1, CCL24, IL-10, or CD-163. We also demonstrate that NOTCH4 signaling modulates IL-13-induced gene expression by increasing IRF4 activity, mediated, at least in part, by the expression of the histone H3K27me3 demethylase JMJD3, and by increasing AP1-dependent transcription. In summary, our results provide evidence for an important role of NOTCH4 signaling in alternative activation of macrophages by IL-13 and suggest that NOTCH4 may contribute to the increased severity of lesions in M2 inflammatory responses, such as allergic asthma, which points to NOTCH4 as a potential new target for the treatment of these pathologies.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"497-509"},"PeriodicalIF":4.4,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9841270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ligand interactions of B cell Siglecs are involved in the prevention of autoimmunity to sialylated self-antigens and in the quality control of signaling-competent B cells. B细胞Siglecs的配体相互作用涉及对唾液酸化自身抗原的自身免疫的预防和信号传导能力B细胞的质量控制。

IF 4.4 4区医学

International immunology Pub Date : 2023-10-06 DOI: 10.1093/intimm/dxad030

Takeshi Tsubata

{"title":"The ligand interactions of B cell Siglecs are involved in the prevention of autoimmunity to sialylated self-antigens and in the quality control of signaling-competent B cells.","authors":"Takeshi Tsubata","doi":"10.1093/intimm/dxad030","DOIUrl":"10.1093/intimm/dxad030","url":null,"abstract":"Sialic acid-binding immunoglobulin-like lectins (Siglecs) are a family of membrane molecules that recognize sialic acid. Most of them are inhibitory receptors that inhibit immune-cell activation by recognizing sialic acid as a self-motif. Human B cells express CD22 (also known as Siglec-2), Siglec-5, Siglec-6 and Siglec-10 whereas mouse B cells express CD22 and Siglec-G (ortholog of human Siglec-10). Siglecs recognize both sialylated molecules expressed on the same cell (cis-ligands) and those expressed by other cells (trans-ligands). In Guillain-Barré syndrome (GBS), antibody production to gangliosides (which are sialic acid-containing glycolipids) expressed by neurons plays a pathogenic role. A Siglec-10 variant deficient in recognition of gangliosides is genetically associated with GBS, suggesting that Siglec-10 induces self-tolerance to gangliosides by recognizing gangliosides as trans-ligands. Recognition of the BCR as a cis-ligand by Siglec-G and CD22 suppresses BCR signaling in B-1 cells and conventional B cells, respectively. This signal suppression prevents excess expansion of B-1 cells and is involved in the quality control of signaling-competent B cells by setting a threshold for tonic signaling during B cell development. CD22 recognizes other cis-ligands including CD22 and β7 integrin. Interaction of CD22 with other CD22 molecules induces CD22 clustering that suppresses CD22-mediated signal inhibition upon BCR ligation, and interaction with β7 integrin maintains its function in the gut-homing of B cells. Taken together, interactions of B cell Siglecs with multiple trans- and cis-ligands play important roles in B cell homeostasis and immune responses.","PeriodicalId":13743,"journal":{"name":"International immunology","volume":" ","pages":"461-473"},"PeriodicalIF":4.4,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10260479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0