改变 B 细胞表位的竞争环境可大大延长抗体的产生时间。

IF 4.8 4区 医学 Q2 IMMUNOLOGY
Hongke Xu, Yanfei Chen, Jingzhi Li, Mengyu Li, Miao Sun, Jian Chen, Ling Li, Qinghong Xue, Hongwei Ma
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引用次数: 0

摘要

持续产生免疫球蛋白 G (IgG) (PIP) 可提供长期疫苗保护。虽然从不同病原体制备的疫苗在保护持续时间上存在差异,但人们对决定 PIP 的因素知之甚少。在这里,我们研究了氨基酸序列、蛋白载体和免疫程序这三个参数对抗肽 IgG 生成持续时间的影响。我们的研究表明,通过使用牛血清白蛋白(BSA)共轭物代替原始病毒颗粒,将含有线性 B 细胞表位的短肽(Pi)置于不同的竞争环境中,抗肽 IgGs 的产生可从瞬时 IgG 产生(TIP)转变为 PIP。用小反刍兽疫(PPR)减毒活疫苗(含有 Pi 作为组成成分)和 BSA-Pi 结合物对山羊进行免疫,抗 Pi IgGs 的产生分别表现出 TIP(持续时间为 368 天)。此外,这种 PIP 不受同一只山羊随后接种 PPR 减毒活疫苗的影响。当山羊同时接种 PPR 减毒活疫苗和 BSA-Pi 时,诱导产生的抗 Pi IgG 的 TIP 稍有延长(从 ~60 天延长到 ~100 天)。这一发现为研究体液免疫反应中浆细胞的命运以及开发与各种病毒线性中和表位相关的多肽疫苗提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Altering the competitive environment of B cell epitopes significantly extends the duration of antibody production.

Persistent immunoglobulin G (IgG) production (PIP) provides long-term vaccine protection. While variations in the duration of protection have been observed with vaccines prepared from different pathogens, little is known about the factors that determine PIP. Here, we investigated the impact of three parameters on the duration of anti-peptide IgG production, namely amino acid sequences, protein carriers, and immunization programs. We show that anti-peptide IgG production can be transformed from transient IgG production (TIP) to PIP, by placing short peptides (Pi) containing linear B cell epitopes in different competitive environments using bovine serum albumin (BSA) conjugates instead of the original viral particles. When goats were immunized with the peste des petits ruminants (PPR) live-attenuated vaccine (containing Pi as the constitutive component) and BSA-Pi conjugate, anti-Pi IgG production exhibited TIP (duration < 60 days) and PIP (duration > 368 days), respectively. Further, this PIP was unaffected by subsequent immunization with the PPR live-attenuated vaccine in the same goat. When goats were coimmunized with PPR live-attenuated vaccine and BSA-Pi, the induced anti-Pi IgG production showed a slightly extended TIP (from ~60 days to ~100 days). This discovery provides new perspectives for studying the fate of plasma cells in humoral immune responses and developing peptide vaccines related to linear neutralizing epitopes from various viruses.

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来源期刊
International immunology
International immunology 医学-免疫学
CiteScore
9.30
自引率
2.30%
发文量
51
审稿时长
6-12 weeks
期刊介绍: International Immunology is an online only (from Jan 2018) journal that publishes basic research and clinical studies from all areas of immunology and includes research conducted in laboratories throughout the world.
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