International Journal of Applied Pharmaceutics最新文献

{"title":"PREPARATION, CHARACTERIZATION AND EVALUATION OF MYRICETIN-LOADED NANOEMULSION FOR THERAPEUTIC EFFICACY IN WOUND HEALING","authors":"Tanvir Y. Shaikh, S. Lodhi","doi":"10.22159/ijap.2024v16i1.49112","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49112","url":null,"abstract":"Objective: Aim of the present study was the development, optimization and evaluation of myricetin-loaded nanoemulsion gel for wound healing.\u0000Methods: Myricetin nanoemulsion was prepared by selecting Peanut oil as oil (wt %), Tween 20 and Polyethylene glycol 400 as surfactant and cosurfactant (Smix) and aqueous phase water. Performance of nanoemulsion gel was evaluated by wound healing activity tested against wound contraction, hydroxyproline content, protein content and antioxidant assay.\u0000Results: The optimized nanoemulsion (NEF1) exhibited appreciable stability concerning droplet size and PDI when stored at 5 ᵒC, 25 ᵒC and 40ᵒC up to three months. Morphological characterization by TEM indicated a spherical shape. Wound healing effect was observed through a significant (p<0.5) increase in hydroxyproline content, protein content and antioxidant status in wound tissue. The level of superoxide dismutase (SOD) and catalase were found to increase significantly in wound tissue after treatment with Myricetin loaded nanoemulsion (MYCT-NE) gel, as well as results were comparable to Betadine cream.\u0000Conclusion: In conclusion, MYCT-NE gel was found potent wound healing effect through the reduction of oxidative stress and epithelialization of tissue.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NOVEL BIOMATERIAL ASSISTED DRUG DELIVERY SYSTEMS FOR THE MANAGEMENT OF ORAL DISEASES–FUTURE THERAPEUTIC APPROACHES","authors":"Mridula R. Chandran, R. Usha","doi":"10.22159/ijap.2024v16i1.49448","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49448","url":null,"abstract":"Oral health is integral to maintaining systemic health as the mouth and oral cavity connect our digestive system with the external environment. The incidence of oro-dental disorders has been emerging as a serious threat to the healthcare sector owing to the increasing complexity of oral microbiome. Conventional treatment modalities are often limited by drug resistance and unwanted inflammatory responses. Recently, therapeutic strategies that can reinstate microbial homeostasis in the oral microenvironment have been implicated in the management of odontogenic infections. Biomaterial-based drug delivery systems, including nanocarriers, dendrimers, hydrogels, oral thin films, oral patches, and other stimuli-responsive polymeric systems, facilitate targeted administration of antimicrobials and anti-inflammatory agents to the site of infection. Bio adhesivity of the polymeric carriers facilitates faster disintegration and accurate dosing of the pharmacological agent to the target site. Moreover, restorative dentistry has been revolutionized by the advent of bio-functional templates that offer improved osseointegration and long-term stability of implants. A comprehensive review of the potential applications of biomaterial-mediated therapeutic strategies in the management of caries, peri-implantitis, periodontitis, and other oro-dental infections is explored here.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139449186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FORMULATION AND BIOPHARMACEUTICAL EVALUATION OF SUSTAINED RELEASE PELLETS OF BOSENTAN BY PANCOATING METHOD","authors":"G. Reddy, P. V, R. P., Ramarao Nadendla","doi":"10.22159/ijap.2024v16i1.49039","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49039","url":null,"abstract":"Objective: The aim of the present study was to formulate sustained-release pellets of bosentan by eudragit RL 100 and RS 100, which are the polymers used in the pan coating technique.\u0000Methods: The sustained release pellets of bosentan were formulated by pan coating method. The drug was coated on nonpareil seeds along with EudragitRL100 by solution layering technique. Drug-loaded pellets were coated with EudragitRS100. The prepared pellets were evaluated for moisture content, drug content, particle size, and in vitro drug release. Stability studies were carried out on the optimised formulations for a period of 6 mo.\u0000Results: The drug content was in the range of 98.89±0.32. The mean particle size of the drug-loaded pellets was in the range of 835 μm. The drug release rate decreased as the concentration of eudragit increased in the pellet formulations. Among the prepared formulations, PC 4 showed 89.35±0.52 drug release in 12 h from a good linear relationship was established between model-independent approaches (T25%, T50%, and T100%) and weight gain in coating. This indicated the possibility of extending the drug release by increasing the weight gain in the coating, and hence, it was proposed to extend the drug release for 24 h. From the prepared pellets, the optimised formulation PC 12 showed a 100.02±0.03 drug release in 24 h. Furthermore, these pellets were filled into capsules and compared the dissolution studies. The compatibility between drugs and polymers in the drug-loaded pellets was confirmed by DSC and FTIR studies. Stability studies indicated that the pellets were stable.\u0000Conclusion: The prepared pellets were capable of releasing the drug for 24 h to treat the Pulmonary Arterial Hypertension.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MODIFIED CYCLODEXTRIN-BASED THERMOSENSITIVE IN SITU GEL FOR VORICONAZOLE OCULAR DELIVERY AGAINST FUNGAL KERATITIS","authors":"S. Sampathi, S. Maddukuri, Ramdas Ramavath, S. Dodoala, V. Kuchana","doi":"10.22159/ijap.2024v16i1.48817","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.48817","url":null,"abstract":"Objective: Fungal keratitis is a severe corneal infection. The present study aims to design and formulate an inclusion complex of Voriconazole-Sulfobutyl ether-beta-cyclodextrin (V-SBECD) loaded thermosensitive in-situ gel to improve solubility, therapy efficacy, durability and reduce the dose-related side effect.\u0000Methods: Poloxamer 407, a thermosensitive polymer along with hydroxypropyl methylcellulose (HPMC E 15), were used as gelling agents; the formulations with poloxamer (16% w/v) and HPMC E15 (1 and 1.5 % w/v) led to a consistent in-situ gel at 37 °C. The formulations were evaluated for drug content, pH, gelation temperature, viscosity, sterility test, antifungal studies, and cell lines studies.\u0000Results: The molar ratio of the drug to SBECD (1:3), showing 42-fold increase in solubility, was chosen to prepare the inclusion complexes using the lyophilization method. The stability constant was found to be 721-m. ATIR peaks, DSC thermograms and NMR spectra indicate the inclusion behavior of Voriconazole and SBECD. In vitro and ex-vivo studies demonstrated that optimized formulation sustained the drug release for over 12 h. Cellular cytotoxicity on Human corneal epithelial cells showed that V-SBECD formulations do not cause corneal epithelial damage after 24 h. In-situ gel and marketed formulation have shown a markable reduction in the growth of the Aspergillus Niger. The optimized SBECD-loaded in-situ gel formulation (F10) did not vary significantly in pH, drug content, viscosity, and % cumulative drug release, signifying stable formulations when tested at 4, 25, and 40 °C.\u0000Conclusion: The research findings envisaged V-SBECD in-situ gel formulation as a concrete strategy to treat severe fungal keratitis.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INVESTIGATING THE TOXICITY OF BETALAIN COMPOUNDS: IN SILICO ANALYSIS AND IN VIVO PREDICTIONS FOR STANDARDIZED BETA VULGARIS L. EXTRACT","authors":"S. E. Nugraha, J. M. Keliat, R. A. Syahputra, Sony Eka, Nugraha","doi":"10.22159/ijap.2024v16i1.49189","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49189","url":null,"abstract":"Objective: Extensive research has been conducted on beetroot's antioxidant, hematoprotective, and cardioprotective properties. However, there currently needs to be more available evidence pertaining to the toxicity assessment of the extract. The toxicity assessment was conducted using both in silico and in vivo methods. Prior to testing, the extracts were standardized in accordance with the guidelines set by the Indonesian Food Drug Authority (BPOM), which is the regulatory authority for food and drugs in Indonesia.\u0000Methods: The experimental subjects consisted of 25 male Wistar rats in good health, weighing between 150 and 170 grams. These rats were separated into five groups, each including five rats. Group 1 will serve as the control group, while groups 2 through 5 will be designated as the treatment groups. The analysis of chemical toxicity was conducted using pK-CSM, SwissADME, and Pro-Tox II methodologies.\u0000Results: The results indicated that the standardized ethanol extract contained 4.341% water, 3.67 % total ash, and 1.53 % acid-insoluble ash. Lead (Pb) and cadmium (Cd) were absent at a concentration of 0 parts per million (ppm). Subsequently, the total plate count and yeast mould count were 0.47 5 x 10-4 (CFU/g) and a of 0.382 x 10-4 (CFU/g) respectively. This finding implies that the extract meets BPOM requirement. This study also measured the betalain content of red beetroot, yielding a total concentration of 11.34 0.37 mg/100 gram of sample. Haematological experiments showed that beetroot extract affected rat blood haematology. Compared to the control group, rats given the extract had higher red blood cell and platelet counts. Additionally, the Insilico toxicity test conducted on the active component derived from beetroot revealed LD50 of the compounds ranged from 305 mg/kg so that were categorized into classes IV and presence of hepatotoxic potential. During the in vivo experiment, there has been a notable rise in hepatic and renal parameters. Furthermore, one mortality event occurred in the test subject at a 5,000 mg/kg body weight dosage.\u0000Conclusion: Single oral administration of the extract at a dose larger than 5,000 mg per kilogram of body weight does not result in lethal effects, however showed potential toxicity to the liver.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LIGAND-BASED VIRTUAL SCREENING OF FDA-APPROVED DRUGS TO IDENTIFY NEW INHIBITORS AGAINST LACTATE DEHYDROGENASE ENZYME OF MALARIA PARASITES","authors":"H. Odhar, Ahmed Fadhil Hashim, S. Humadi, S. Ahjel","doi":"10.22159/ijap.2024v16i1.49382","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49382","url":null,"abstract":"Objective: The aim of this study is to computationally repurpose FDA-approved drugs as potential inhibitors of the Plasmodium falciparum lactate dehydrogenase (PfLDH) by competing with the cofactor NADH.\u0000Methods: In this in silico study, we have virtually screened a library of FDA-approved drugs for structural similarity to the dihydro nicotinamide adenine dinucleotide (NADH). Then, the top hits were further assessed for clinical safety and by application of molecular docking and dynamics simulation.\u0000Results: Ligand-based virtual screening reports that the antibiotic Novobiocin has a good similarity to the cofactor NADH with a score of 0.7. Also, molecular docking study indicates that Novobiocin may has the ability to interact with PfLDH enzyme with a docking energy of-8.8 Kcal/mol. However, during molecular dynamics (MD) simulation, the mean ligand proximity root mean square deviation (RMSD) and binding energy for Novobiocin were 4.3 Angstrom and-37.45 Kcal/mol, respectively. These MD simulation parameters are inferior to those recorded for NADH molecule during 50 nanosecond intervals.\u0000Conclusion: The antibiotic Novobiocin may serve as a potential lead candidate toward the design of novel antimalarial agents. However, further evaluation of Novobiocin may be recommended to affirm its capacity against PfLDH enzyme.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NANOPARTICLE PREPARATION OF SIAM CITRUS PEEL EXTRACT (CITRUS NOBILIS L. VAR. MICROCARPA) USING SHORT-CHAIN CHITOSAN AND TRIPOLYPHOSPHATE AS CROSS LINKER AND CELLULAR UPTAKE STUDY ON MCF-7 CELL LINE BY IN VITRO","authors":"Wintari Taurina, Mohamad Andrie","doi":"10.22159/ijap.2024v16i1.49487","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49487","url":null,"abstract":"Objective: High consumption of oranges causes a lot of orange peel waste. Orange peel contains the compound naringenin, which has a cytotoxic effect on various cancer cells. This research aims to develop a preparation of Siamese orange peel extract nanoparticles with short-chain chitosan and tripolyphosphate carriers as an oral drug delivery system and determine its cytotoxic activity against the Michigan Cancer Foundation-7 (MCF-7) cell line.\u0000Methods: This research uses the micro tetrazolium (MTT) method to see the cytotoxic activity extract of methanol obtained from maceration extraction. The extract was then formulated into nanoparticles using chitosan and tripolyphosphate. Characterization and evaluation of nanoparticles were carried out, including particle size, zeta potential, entrapment efficiency, and stability in the stomach using 0.1 N HCl and in the intestine using Artificial Intestinal Fluid (AIF) in vitro. This research was also conducted to assess the ability of nanoparticles to enter MCF-7 cells (cellular uptake).\u0000Results: Nanoparticles were successfully developed from Siamese orange peel extract. The results of the day 0 nanoparticle characterization were spherical, with average particle size 284.3 nm, zeta potential 0.713 mV, entrapment efficiency 96.73%, and stability in 0.1 N HCl at the 0th hours, respectively. 1st, 2nd, and 3rd. 99.16%, 98.70%, 98.47%, 98.31%, stability on AIF at hours 0, 1, 2, 3 and 4 respectively 99.52%, 99.30%, 99.40%, 98.99%, 99.29%. Characterization of nanoparticles on day 25 showed that the average particle size was 196.2 nm, zeta potential 0.476 mV, entrapment efficiency 96.92%, stability in 0.1 N HCl at 0, 1, 2 and 3 h respectively 99.51%, 98.67%, 98.51%, 98.27%, stability in AIF at 0th, 1st, 2nd, 3rd, and 4th hours 99.24 respectively %, 98.76%, 98.46%, 97.93%, 97.58%. Cytotoxic activity of extract Siamese citrus peel against MCF-7 cells with IC50 of 290.58 µg/ml. The result shows that cellular uptake of Siamese citrus peel nanoparticles can penetrate MCF-7 cells.\u0000Conclusion: Stable nanoparticles were successfully developed from Siamese orange peel extract, and their stability was maintained throughout a 30-day storage period. This extract displayed cytotoxic effects and showcased the ability for cellular uptake in MCF-7 cell cultures in vitro.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BI-LAMINATED ORAL DISINTEGRATING FILM FOR SYMPTOMATIC TREATMENT OF VIRAL NASOPHARYNGITIS: FORMULATION, CHARACTERIZATION, TASTE MASKING, AND STABILITY STUDIES","authors":"Merna A. Rizk, M. Teaima, Rehab Abdelmonem, M. EL-NABARAWI, S. F. Elhabal","doi":"10.22159/ijap.2024v16i1.49499","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49499","url":null,"abstract":"Objective: Nasopharyngitis (NP) is one of the most common upper respiratory tract infections (URTIs) brought on by viral infections and requires symptomatic treatment. In this study, a new approach to delivering drugs was developed, which is a bi-laminated oral disintegrating film (ODF) containing dextromethorphan hydrobromide (DEX), phenylephrine hydrochloride (PE), and methylcobalamin (MeCbl) to support the symptomatic treatment of NP. Yet, the extreme bitterness of DEX and PE required a taste-masking technique before ODF formulation to enhance patient compliance.\u0000Methods: Various complexing agents were tried at different ratios to mask the bitter taste of the drug(s). DEX-PE ODFs were formulated using the solvent casting procedure. A 31.21 full factorial design was performed to characterize DEX-PE ODFs, where the effects of polymer grade and plasticizer type were evaluated on the disintegration time (DT) and the percentage of drugs released after 10 min (Q10).\u0000Results: Complexation with maltodextrin (MD) and ion exchange resin (Kyron T-314) could successfully mask the bitter taste at a ratio of 1:2:2 of drugs, MD, and Kyron T-314, respectively. The ODF optimized formula (F4) recorded the least DT (5±0.5 s) and highest Q10 (96.7±2.1%) and (97.4±1.9%) for DEX and PE, respectively. F4 was then combined with the second film layer containing MeCbl to develop the bi-laminated ODF (B1), which was later evaluated and subjected to stability studies.\u0000Conclusion: In conclusion, a taste-masked, bi-laminated ODF could be successfully developed for the symptomatic treatment of NP.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NAVIGATING THE LANDSCAPE OF ADJUVANTS FOR SUBUNIT VACCINES: RECENT ADVANCES AND FUTURE PERSPECTIVES","authors":"Fredmoore L. Orosco, Llewelyn M. Espiritu","doi":"10.22159/ijap.2024v16i1.49563","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49563","url":null,"abstract":"The development of effective subunit vaccines relies on the incorporation of adjuvants to enhance immune responses and improve vaccine efficacy. This paper provides a comprehensive review of the various adjuvants employed in subunit vaccine development, with an emphasis on liposome-based, carbohydrate-based, polymer-based, and nanoparticle-based adjuvants. Additionally, the general concept of vaccine adjuvants, their classification into different types, and the underlying molecular mechanisms by which they exert their immunostimulatory effects are discussed. The use of adjuvants in subunit vaccine development has revolutionized immunization strategies by enhancing vaccine efficacy and inducing robust immune responses. Further research is needed to understand the safety profiles of adjuvants, elucidate the underlying molecular mechanisms, and optimize the adjuvant formulations. By harnessing the power of adjuvants, we can advance the development of effective subunit vaccines against infectious diseases and malignancies, thereby contributing to global health outcomes.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139449157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FORMULATION AND ASSESSMENT OF HERBAL EMULGELS IN THE MANAGEMENT OF ACNE: IN VITRO AND IN VIVO INVESTIGATIONS","authors":"Swapna Neela, Makula Ajitha, V. Kuchana","doi":"10.22159/ijap.2024v16i1.49671","DOIUrl":"https://doi.org/10.22159/ijap.2024v16i1.49671","url":null,"abstract":"Objective: The main objective of the current research was to prepare herbal emulgel and analyze the effect of herbal formulation in the treatment of acne.\u0000Methods: The plants Tabernaemontana coronaria and Thunbergia alata were selected for the study because of folklore for their medicinal values. The T. coronaria and T. alata test extracts were prepared by soxhlet extraction procedure and subjected to physico-chemical evaluation. The formulated herbal emulgels prepared by dispersion technique were investigated for anti-acne properties by in vitro and in vivo methods. The prepared emulgel formulations were assessed for parameters like viscosity, spreadability, pH, content uniformity, stickiness, zeta potential, particle size, surface morphology, and in vitro diffusion studies.\u0000Results: The physico-chemical evaluation of herbal gel revealed that emulgel appeared light green in colour, opaque, and odourless with smooth texture. The emulgels of both the test extracts showed no stickiness, and revealed pH ranging from 5.467±0.13 to 5.889±0.1. When the shear rate was increased, there was a decrease in the viscosity of the test emulgels, with good extrudability. The content uniformity of F5 emulgel for T. coronaria and T. alatawas 99%, and spreadibilty was more with F7 formulation of T. coronaria and F6 formulation of T. alata, respectively. In the stability testing studies, amongst all the formulations prepared, F5 was found to be stable upon storage for six months. In vitro studies, F5 formulation of both the test extracts had a remarkable zone of inhibition; whereas F5 formulation treated histopathological sections in in vivo investigation displayed a decline in the overall damage induced by Propionibacterium acnes. The results showed no statistical significant difference for measurement of zone of inhibition and histopthological studies between the test formulations and standard drug.\u0000Conclusion: The study concludes that both herbal formulations were promising agents for the treatment of acne vulgaris.","PeriodicalId":13737,"journal":{"name":"International Journal of Applied Pharmaceutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}