制备、表征和评估负载 myricetin 的纳米乳液对伤口愈合的疗效

Q2 Pharmacology, Toxicology and Pharmaceutics

International Journal of Applied Pharmaceutics Pub Date : 2024-01-07 DOI:10.22159/ijap.2024v16i1.49112

Tanvir Y. Shaikh, S. Lodhi

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引用次数: 0

摘要

研究目的本研究的目的是开发、优化和评估用于伤口愈合的杨梅素纳米乳液凝胶：方法：选用花生油作为油（重量百分比），吐温 20 和聚乙二醇 400 作为表面活性剂和助表面活性剂（Smix），水相为水，制备出米格列汀纳米乳液。纳米乳液凝胶的性能通过伤口收缩、羟脯氨酸含量、蛋白质含量和抗氧化测定的伤口愈合活性进行了评估：结果：优化后的纳米乳液（NEF1）在 5 ᵒC、25 ᵒC、40 ᵒC条件下储存三个月后，在液滴大小和 PDI 方面表现出明显的稳定性。用 TEM 进行的形态学表征表明，该材料呈球形。通过观察伤口组织中羟脯氨酸含量、蛋白质含量和抗氧化状态的显著增加（p<0.5），可以发现伤口愈合效果。经 Myricetin 负载纳米乳液（MYCT-NE）凝胶处理后，伤口组织中的超氧化物歧化酶（SOD）和过氧化氢酶的水平明显增加，其结果与倍他丁乳膏相当：总之，MYCT-NE 凝胶通过减少氧化应激和组织上皮化，具有强大的伤口愈合效果。

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PREPARATION, CHARACTERIZATION AND EVALUATION OF MYRICETIN-LOADED NANOEMULSION FOR THERAPEUTIC EFFICACY IN WOUND HEALING

Objective: Aim of the present study was the development, optimization and evaluation of myricetin-loaded nanoemulsion gel for wound healing. Methods: Myricetin nanoemulsion was prepared by selecting Peanut oil as oil (wt %), Tween 20 and Polyethylene glycol 400 as surfactant and cosurfactant (Smix) and aqueous phase water. Performance of nanoemulsion gel was evaluated by wound healing activity tested against wound contraction, hydroxyproline content, protein content and antioxidant assay. Results: The optimized nanoemulsion (NEF1) exhibited appreciable stability concerning droplet size and PDI when stored at 5 ᵒC, 25 ᵒC and 40ᵒC up to three months. Morphological characterization by TEM indicated a spherical shape. Wound healing effect was observed through a significant (p<0.5) increase in hydroxyproline content, protein content and antioxidant status in wound tissue. The level of superoxide dismutase (SOD) and catalase were found to increase significantly in wound tissue after treatment with Myricetin loaded nanoemulsion (MYCT-NE) gel, as well as results were comparable to Betadine cream. Conclusion: In conclusion, MYCT-NE gel was found potent wound healing effect through the reduction of oxidative stress and epithelialization of tissue.

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来源期刊

International Journal of Applied Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

219

期刊介绍： International Journal of Applied Pharmaceutics (Int J App Pharm) is a peer-reviewed, bimonthly (onward March 2017) open access journal devoted to the excellence and research in the pure pharmaceutics. This Journal publishes original research work that contributes significantly to further the scientific knowledge in conventional dosage forms, formulation development and characterization, controlled and novel drug delivery, biopharmaceutics, pharmacokinetics, molecular drug design, polymer-based drug delivery, nanotechnology, nanocarrier based drug delivery, novel routes and modes of delivery; responsive delivery systems, prodrug design, development and characterization of the targeted drug delivery systems, ligand carrier interactions etc. However, the other areas which are related to the pharmaceutics are also entertained includes physical pharmacy and API (active pharmaceutical ingredients) analysis. The Journal publishes original research work either as a Original Article or as a Short Communication. Review Articles on a current topic in the said fields are also considered for publication in the Journal.