FORMULATION AND ASSESSMENT OF HERBAL EMULGELS IN THE MANAGEMENT OF ACNE: IN VITRO AND IN VIVO INVESTIGATIONS

Q2 Pharmacology, Toxicology and Pharmaceutics

International Journal of Applied Pharmaceutics Pub Date : 2024-01-07 DOI:10.22159/ijap.2024v16i1.49671

Swapna Neela, Makula Ajitha, V. Kuchana

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Abstract

Objective: The main objective of the current research was to prepare herbal emulgel and analyze the effect of herbal formulation in the treatment of acne. Methods: The plants Tabernaemontana coronaria and Thunbergia alata were selected for the study because of folklore for their medicinal values. The T. coronaria and T. alata test extracts were prepared by soxhlet extraction procedure and subjected to physico-chemical evaluation. The formulated herbal emulgels prepared by dispersion technique were investigated for anti-acne properties by in vitro and in vivo methods. The prepared emulgel formulations were assessed for parameters like viscosity, spreadability, pH, content uniformity, stickiness, zeta potential, particle size, surface morphology, and in vitro diffusion studies. Results: The physico-chemical evaluation of herbal gel revealed that emulgel appeared light green in colour, opaque, and odourless with smooth texture. The emulgels of both the test extracts showed no stickiness, and revealed pH ranging from 5.467±0.13 to 5.889±0.1. When the shear rate was increased, there was a decrease in the viscosity of the test emulgels, with good extrudability. The content uniformity of F5 emulgel for T. coronaria and T. alatawas 99%, and spreadibilty was more with F7 formulation of T. coronaria and F6 formulation of T. alata, respectively. In the stability testing studies, amongst all the formulations prepared, F5 was found to be stable upon storage for six months. In vitro studies, F5 formulation of both the test extracts had a remarkable zone of inhibition; whereas F5 formulation treated histopathological sections in in vivo investigation displayed a decline in the overall damage induced by Propionibacterium acnes. The results showed no statistical significant difference for measurement of zone of inhibition and histopthological studies between the test formulations and standard drug. Conclusion: The study concludes that both herbal formulations were promising agents for the treatment of acne vulgaris.

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配制和评估用于治疗痤疮的草药凝胶：体外和体内研究

研究目的本研究的主要目的是制备草药凝胶，并分析草药配方在治疗痤疮方面的效果：研究选取了民间传说中具有药用价值的 Tabernaemontana coronaria 和 Thunbergia alata 植物。通过索氏提取法制备了 T. coronaria 和 T. alata 的试验提取物，并对其进行了理化评价。采用分散技术制备的草药凝胶配方通过体外和体内方法研究了其抗痤疮特性。对所制备的乳凝胶配方的粘度、涂抹性、pH 值、含量均匀性、粘性、zeta 电位、粒度、表面形态和体外扩散研究等参数进行了评估：草药凝胶的理化评估显示，凝胶呈淡绿色，不透明，无味，质地光滑。两种试验提取物的凝胶均无粘性，pH 值范围为 5.467±0.13 至 5.889±0.1。当剪切速率增加时，试验乳胶的粘度下降，挤出性良好。冠突散囊菌和白头翁的 F5 含量均匀度为 99%，冠突散囊菌的 F7 和白头翁的 F6 含量均匀度更高。在稳定性测试研究中，在所有制备的制剂中，F5 在储存 6 个月后保持稳定。在体外研究中，两种测试提取物的 F5 制剂都有显著的抑制区；而在体内研究中，经 F5 制剂处理的组织病理切片显示，痤疮丙酸杆菌引起的整体损伤有所下降。结果表明，在抑制区的测量和组织病理学研究方面，试验配方与标准药物之间没有明显的统计学差异：研究得出结论，两种草药配方都是治疗寻常痤疮的有效药物。

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来源期刊

International Journal of Applied Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

219

期刊介绍： International Journal of Applied Pharmaceutics (Int J App Pharm) is a peer-reviewed, bimonthly (onward March 2017) open access journal devoted to the excellence and research in the pure pharmaceutics. This Journal publishes original research work that contributes significantly to further the scientific knowledge in conventional dosage forms, formulation development and characterization, controlled and novel drug delivery, biopharmaceutics, pharmacokinetics, molecular drug design, polymer-based drug delivery, nanotechnology, nanocarrier based drug delivery, novel routes and modes of delivery; responsive delivery systems, prodrug design, development and characterization of the targeted drug delivery systems, ligand carrier interactions etc. However, the other areas which are related to the pharmaceutics are also entertained includes physical pharmacy and API (active pharmaceutical ingredients) analysis. The Journal publishes original research work either as a Original Article or as a Short Communication. Review Articles on a current topic in the said fields are also considered for publication in the Journal.