Innate Immunity最新文献_第8页

TLRs in COVID-19: How they drive immunopathology and the rationale for modulation. tlr在COVID-19中的作用:它们如何驱动免疫病理以及调节的基本原理。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-10-01 Epub Date: 2021-11-20 DOI: 10.1177/17534259211051364

F Linzee Mabrey, Eric D Morrell, Mark M Wurfel

引用次数: 24

Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways. 黄脾醇D通过TLR4-MAPKs/NF-κB信号通路抑制氧化应激、炎症和凋亡，保护小鼠免受lps诱导的急性肺损伤。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-10-01 Epub Date: 2021-11-20 DOI: 10.1177/17534259211051069

Qinqin Zhang, Aozi Feng, Mengnan Zeng, Beibei Zhang, Jingya Shi, Yaxin Lv, Bing Cao, Chenxin Zhao, Mengya Wang, Yifan Ding, Xiaoke Zheng

{"title":"Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways.","authors":"Qinqin Zhang, Aozi Feng, Mengnan Zeng, Beibei Zhang, Jingya Shi, Yaxin Lv, Bing Cao, Chenxin Zhao, Mengya Wang, Yifan Ding, Xiaoke Zheng","doi":"10.1177/17534259211051069","DOIUrl":"10.1177/17534259211051069","url":null,"abstract":"This study investigated the effect and mechanism of chrysosplenol D (CD) on LPS-induced acute lung injury in mice. Histological changes in the lungs were measured by hematoxylin-eosin staining. The levels of IL-6, IL-1β, and TNF-α in the bronchoalveolar lavage fluid were detected by ELISA. The levels of oxidative stress were detected by the cuvette assay. Immune cells in peripheral blood, the levels of reactive oxygen species, and apoptosis of primary lung cells were detected by flow cytometry. The mRNA levels of TLR4, MyD88, IL-1β, and NLRP3 were measured by quantitative real-time polymerase chain reaction. The levels of proteins in apoptosis and the TLR4-MAPKs/NF-κB signaling pathways were detected by Western blot. Hematoxylin-eosin staining showed that CD could improve lung injury; decrease the levels of inflammatory factors, oxidative stress, reactive oxygen species, and cell apoptosis; and regulate the immune system. Moreover, CD could down-regulate the mRNA levels of TLR4, MyD88, NLRP3, and IL-1β in lung, and the protein levels of Keap-1, Cleaved-Caspase-3/Caspase-3, Cleaved-Caspase-9/Caspase-9, TLR4, MyD88, p-ERK/ERK, p-JNK/JNK, p-p38/p38, p-p65/p65, NLRP3, and IL-1β, and up-regulated the levels of Bcl-2/Bax, p-Nrf2/Nrf2, and HO-1. The results suggested that CD could protect mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via the TLR4-MAPKs/NF-κB signaling pathways.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 7-8","pages":"514-524"},"PeriodicalIF":3.2,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/89/10.1177_17534259211051069.PMC8762090.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39645610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Circulating innate lymphoid cell subtypes and altered cytokine profiles following an atherogenic high-fat diet. 循环先天性淋巴细胞亚型和致动脉粥样硬化高脂饮食后细胞因子谱的改变。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-10-01 Epub Date: 2021-11-17 DOI: 10.1177/17534259211053634

Vuyolwethu Mxinwa, Phiwayinkosi V Dludla, Tawanda M Nyambuya, Bongani B Nkambule

{"title":"Circulating innate lymphoid cell subtypes and altered cytokine profiles following an atherogenic high-fat diet.","authors":"Vuyolwethu Mxinwa, Phiwayinkosi V Dludla, Tawanda M Nyambuya, Bongani B Nkambule","doi":"10.1177/17534259211053634","DOIUrl":"10.1177/17534259211053634","url":null,"abstract":"Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid cell (ILC) profiles in a state of impaired Glc tolerance. Sixteen male C57BL/6 mice were randomized to receive two experimental diets (n = 8 per group), low-fat (LFD), and HFD for 8-13 wk. We evaluated the levels of circulating innate lymphoid cells and their respective cytokines following HFD-feeding. The HFD group had impaired Glc tolerance, elevated insulin levels, and increased total cholesterol levels. Notably, the levels of circulating ILC1s were elevated following 13 wk of HFD-feeding. Moreover, the levels of TNF-α were decreased, but there were no changes in IFN-γ levels. Lastly, the levels of circulating ILC2s and ILC3s were comparable between the HFD and LFD group. The findings demonstrated that short-term HFD-feeding increases postprandial blood Glc, total cholesterol and insulin levels. However, the metabolic changes did not alter ILC2 and ILC3 levels and their respective cytokine profiles.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 7-8","pages":"525-532"},"PeriodicalIF":3.2,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/1e/10.1177_17534259211053634.PMC8762092.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39631154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Lys694Arg polymorphism leads to blunted responses to LPS by interfering TLR4 with recruitment of MyD88. Lys694Arg多态性通过干扰TLR4与MyD88的募集而导致对LPS的迟钝反应。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2020-06-08 DOI: 10.1177/1753425920927479

Yajie Yang, Yan Hu, Yile Zhou, Tao Liang, Haihong Tang, Huihui Ju, Qiqing Shi, Hao Fang

{"title":"Lys694Arg polymorphism leads to blunted responses to LPS by interfering TLR4 with recruitment of MyD88.","authors":"Yajie Yang, Yan Hu, Yile Zhou, Tao Liang, Haihong Tang, Huihui Ju, Qiqing Shi, Hao Fang","doi":"10.1177/1753425920927479","DOIUrl":"https://doi.org/10.1177/1753425920927479","url":null,"abstract":"TLR4 polymorphisms such as Asp299Gly and Thr399Ile related to Gram-negative sepsis have been reported to result in significantly blunted responsiveness to LPS. Our study group previously screened other TLR4 polymorphic variants by checking the NF-κB activation in comparison to wild type (WT) TLR4 in human embryonic kidney 293T cells. In this study, we found that the Lys694Arg (K694R) polymorphism reduced the activation of NF-κB, and the production of downstream inflammatory factors IL-1, TNF-α and IL-6, representing the K694R polymorphism, led to blunted responsiveness to LPS. Then, we examined the influence of the K694R polymorphism on total and cell-surface TLR4 expression by Western blotting and flow cytometry, respectively, but observed no differences between the K694R polymorphism and WT TLR4. We also used co-immunoprecipitation to determine the interaction of the K694R polymorphism and WT TLR4 with their co-receptor myeloid differentiation factor 2 (MD2) and their downstream signal adaptor MyD88. We found that K694R reduced the recruitment of MyD88 in TLR4 signalling but had no impact on the interaction with MD2.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"483-492"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920927479","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38027226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants. 巨噬细胞中的IRAK-4有助于髋关节植入物松动周围磨损颗粒的炎症性骨溶解。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-06-18 DOI: 10.1177/17534259211018740

Yang-Chun Zhang, Jian-Hong Xiao, Shao-Jie Deng, Guo-Liang Yi

{"title":"IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants.","authors":"Yang-Chun Zhang, Jian-Hong Xiao, Shao-Jie Deng, Guo-Liang Yi","doi":"10.1177/17534259211018740","DOIUrl":"https://doi.org/10.1177/17534259211018740","url":null,"abstract":"TLRs recognizing PAMPS play a role in local immunity and participate in implant-associated loosening. TLR-mediated signaling is primarily regulated by IL-1 receptor associated kinase-M (IRAK-M) negatively and IRAK-4 positively. Our previous studies have proved that wear particles promote endotoxin tolerance in macrophages by inducing IRAK-M. However, whether IRAK-4 is involved in inflammatory osteolysis of wear particles basically, and the specific mechanism of IRAK-4 around loosened hip implants, is still unclear. IRAK-4 was studied in the interface membranes from patients in vivo and in particle-stimulated macrophages to clarify its role. Also, IL-1β and TNF-α levels were measured after particle and LPS stimulation in macrophages with or without IRAK-4 silenced by siRNA. Our results showed that the interface membranes around aseptic and septic loosened prosthesis expressed more IRAK-4 compared with membranes from osteoarthritic patients. IRAK-4 in macrophages increased upon particle and LPS stimulation. In the former, IL-1β and TNF-α levels were lower compared with those of LPS stimulation, and IRAK-4 siRNA could suppress production of pro-inflammatory cytokines. These findings suggest that besides IRAK-M, IRAK-4 also plays an important role in the local inflammatory reaction and contributes to prosthesis loosening.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"470-482"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17534259211018740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39241955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Acanthopanax senticosus supplementation on innate immunity and changes of related immune factors in healthy mice. 补充刺五加对健康小鼠先天免疫的影响及相关免疫因子的变化。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2020-09-16 DOI: 10.1177/1753425920955200

YunQiang Zhang, YunLu Zhang, ZiKui Liu

{"title":"Effects of Acanthopanax senticosus supplementation on innate immunity and changes of related immune factors in healthy mice.","authors":"YunQiang Zhang, YunLu Zhang, ZiKui Liu","doi":"10.1177/1753425920955200","DOIUrl":"https://doi.org/10.1177/1753425920955200","url":null,"abstract":"Modern scientific research has shown that Acanthopanax senticosus (AS) can regulate the innate immunity of healthy animals, thus affecting the health of animals. However, there are few systematic reports on the changes of innate immune indices of healthy animals after consuming AS. The purpose of this project was to study the effect on healthy mice's innate immunity and changes of related immune factors induced by feeding AS root powder supplementation. The results showed that the killing rate of natural cells increased in a dose-dependent manner in a certain time period. Compared to the control group, the treatment groups (T1, T2 and T3) improved significantly in the innate immune index (lysozyme, β-defensin-2 and duodenal secretory IgA (SIgA) to varying degrees) and induced corresponding changes of immune factors at certain time periods. The correlation between SIgA and IFN-γ in mouse serum was enhanced, and the higher the concentration of AS in the diet, the stronger the correlation was. However, there was no significant difference in growth performance among groups. It is proved that AS supplementation can enhance innate immunity and change several relevant immune factors and cells of healthy mice without affecting growth performance.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"461-469"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920955200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38386762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation. 沃尔夫-赫希霍恩综合征候选病例 1 通过 NEK7 介导的 NLRP3 炎性体激活，在脓毒症诱发的急性肺损伤中促进肺泡巨噬细胞的脓毒症。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-08-25 DOI: 10.1177/17534259211035426

Caixia Liu, Benlong Cai, Dan Li, Yuan Yao

{"title":"Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.","authors":"Caixia Liu, Benlong Cai, Dan Li, Yuan Yao","doi":"10.1177/17534259211035426","DOIUrl":"10.1177/17534259211035426","url":null,"abstract":"Sepsis is a complex clinical syndrome with high incidence and mortality. Acute lung injury (ALI) is a common complication of sepsis. At present, there is no effective therapeutic strategy to treat ALI. The SET domain-containing histone methyltransferase Wolf-Hirschhorn syndrome candidate 1 (WHSC1) regulates cancer progression, while its role in sepsis-induced ALI remains unclear. Thus, this study aimed to study the effect of WHSC1 on sepsis-induced ALI and to explore the potential mechanism of action. In the study, LPS treatment induced lung injury. WHSC1 was highly expressed in LPS-induced ALI. Knockdown of WHSC1 attenuated LPS-induced ALI and pyroptosis in vivo. Besides, knockdown of WHSC1 attenuated LPS-induced alveolar macrophage pyroptosis in vitro. Furthermore, NIMA-related kinase-7 (NEK7) expression could be regulated by WHSC1, and NEK7 bound to NLRP3 in alveolar macrophages. Moreover, WHSC1 regulated alveolar macrophage pyroptosis through modulating NEK7-mediated NLRP3 inflammasome activation. In conclusion, WHSC1 was highly expressed in LPS-induced ALI. WHSC1 facilitated alveolar macrophage pyroptosis in sepsis-induced ALI through NEK7-mediated NLRP3 inflammasome activation. WHSC1 may be a valuable target for the therapy of sepsis-induced ALI.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"437-447"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/af/10.1177_17534259211035426.PMC8504266.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39340831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neglected roles of IgG Fc-binding protein secreted from airway mucin-producing cells in protecting against SARS-CoV-2 infection. 气道粘液生成细胞分泌的IgG fc结合蛋白在预防SARS-CoV-2感染中的作用被忽视。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-09-15 DOI: 10.1177/17534259211043159

Kensuke Kobayashi, Mitsuhiro Tachibana, Yutaka Tsutsumi

{"title":"Neglected roles of IgG Fc-binding protein secreted from airway mucin-producing cells in protecting against SARS-CoV-2 infection.","authors":"Kensuke Kobayashi, Mitsuhiro Tachibana, Yutaka Tsutsumi","doi":"10.1177/17534259211043159","DOIUrl":"https://doi.org/10.1177/17534259211043159","url":null,"abstract":"Both innate immunity and acquired immunity are involved in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The induction of Abs that neutralize the virus has been described, and certain Abs against endemic coronaviruses may cross-react with SARS-CoV-2. Detailed mechanisms to protect against the pandemic of SARS-CoV-2 remain unresolved. We previously reported that IgG Fc-binding protein (Fcγbp), a unique, large molecular weight, and mucin-like secretory Fc receptor protein, secreted from goblet cells of human small and large intestine, mediates the transportation of serum IgG onto the mucosal surface. In this review, we show that mucous bronchial gland cells and some goblet cells are immunoreactive for Fcγbp. Fcγbp traps the cross-reactive (both neutralizing and non-neutralizing) IgG bound to the virus and can consequently eliminate the virus from the mucosal surface to decrease viral loads. Fcγbp can also suppress immune overreaction by interfering with Fc-binding by macrophages and competing with complement fixation. Fcγbp secreted from mucin-producing cells of the airway functions as an important anti-infection mucosal defense. The Fcγbp-mediated mechanism can be a key factor in explaining why SARS-CoV-2 is less infective/lethal in children, and may also be involved in the unique Ab response, recurrent infection, and effects of serum therapy and vaccination.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"423-436"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/90/10.1177_17534259211043159.PMC8504265.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39415547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Differential induction of innate memory in porcine monocytes by β-glucan or bacillus Calmette-Guerin. β-葡聚糖和卡介苗芽孢杆菌对猪单核细胞先天记忆的差异诱导。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2020-08-30 DOI: 10.1177/1753425920951607

Kristen A Byrne, Christopher K Tuggle, Crystal L Loving

{"title":"Differential induction of innate memory in porcine monocytes by β-glucan or bacillus Calmette-Guerin.","authors":"Kristen A Byrne, Christopher K Tuggle, Crystal L Loving","doi":"10.1177/1753425920951607","DOIUrl":"https://doi.org/10.1177/1753425920951607","url":null,"abstract":"Innate immunomodulation via induction of innate memory is one mechanism to alter the host's innate immune response to reduce or prevent disease. Microbial products modulate innate responses with immediate and lasting effects. Innate memory is characterized by enhanced (training) or depressed (tolerance) innate immune responses, including pro-inflammatory cytokine production, to secondary exposure following a priming event. To investigate the ability of β-glucans and bacillus Calmette-Guerin to induce innate training or tolerance in pig cells, porcine monocytes were cultured with priming agonist (β-glucans or bacillus Calmette-Guerin) then re-stimulated 5 d later with a heterologous microbial agonist to determine induction of innate memory. Priming with β-glucan from Saccharomyces cerevisiae depressed IL-1β and TNF-α cytokine responses to re-stimulation with LPS, indicative of a tolerized state. However, bacillus Calmette-Guerin priming induced a trained state in porcine monocytes, as LPS re-stimulation enhanced IL-1β and TNF-α gene expression and protein production. We present the first evidence of innate memory in pig monocytes, with bacillus Calmette-Guerin (training) or Saccharomyces cerevisiae β-glucan (tolerance). Induction of a trained or tolerized state in vitro is a first step to identify agonists to alter the innate immune system at the animal level with the intent of enhancing disease resistance.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"448-460"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920951607","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38419990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis. TLR4和TLR9多态性:在比哈尔邦印度内脏利什曼病人群中易感性的可能作用

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-04-28 DOI: 10.1177/1753425920965658

Abhishek Mandal, Manish Kumar, Ashish Kumar, Abhik Sen, Pradeep Das, Sushmita Das

{"title":"TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis.","authors":"Abhishek Mandal, Manish Kumar, Ashish Kumar, Abhik Sen, Pradeep Das, Sushmita Das","doi":"10.1177/1753425920965658","DOIUrl":"https://doi.org/10.1177/1753425920965658","url":null,"abstract":"Genetic variations in the host TLRs genes play an important role in susceptibility and/or resistance to visceral leishmaniasis by altering the host-pathogen interaction. In this study, we investigated the association between polymorphisms of TLR4 (Asp299Gly, Thr399Ile) and TLR-9 (T-1237C), with susceptibility to visceral leishmaniasis. A bi-directional PCR amplification of specific alleles technique was used to characterize the distribution of TLR4 (Asp299Gly and Thr399Ile) and TLR9 (T-1237C) polymorphisms. A total of 60 samples were randomly selected from confirmed visceral leishmaniasis patients and 24 endemic healthy volunteers. The samples were genotyped and allele frequencies were determined. We observed that TLR4 Asp299Gly and Thr399Ile genotypes were more frequent in visceral leishmaniasis patients (10% and 15% respectively) compared to controls (4.2% and 8.3% respectively). However, the differences were not significant in TLR4 Asp299Gly and Thr399Ile alleles and genotypes. In the case of TLR9, we observed the frequency of T1237C genotype was higher in visceral leishmaniasis patients (43.3%) than in healthy controls (33.3%). Statistically significant differences were observed in TLR9 T1237C alleles and genotypes. We concluded that TLR9 T1237C, but not TLR4, gene polymorphisms can be regarded as contributors to visceral leishmaniasis susceptibility among the Indian population of Bihar state.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"493-500"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920965658","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38917749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1