Innate Immunity最新文献_第9页

IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants. 巨噬细胞中的IRAK-4有助于髋关节植入物松动周围磨损颗粒的炎症性骨溶解。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-06-18 DOI: 10.1177/17534259211018740

Yang-Chun Zhang, Jian-Hong Xiao, Shao-Jie Deng, Guo-Liang Yi

{"title":"IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants.","authors":"Yang-Chun Zhang, Jian-Hong Xiao, Shao-Jie Deng, Guo-Liang Yi","doi":"10.1177/17534259211018740","DOIUrl":"https://doi.org/10.1177/17534259211018740","url":null,"abstract":"TLRs recognizing PAMPS play a role in local immunity and participate in implant-associated loosening. TLR-mediated signaling is primarily regulated by IL-1 receptor associated kinase-M (IRAK-M) negatively and IRAK-4 positively. Our previous studies have proved that wear particles promote endotoxin tolerance in macrophages by inducing IRAK-M. However, whether IRAK-4 is involved in inflammatory osteolysis of wear particles basically, and the specific mechanism of IRAK-4 around loosened hip implants, is still unclear. IRAK-4 was studied in the interface membranes from patients in vivo and in particle-stimulated macrophages to clarify its role. Also, IL-1β and TNF-α levels were measured after particle and LPS stimulation in macrophages with or without IRAK-4 silenced by siRNA. Our results showed that the interface membranes around aseptic and septic loosened prosthesis expressed more IRAK-4 compared with membranes from osteoarthritic patients. IRAK-4 in macrophages increased upon particle and LPS stimulation. In the former, IL-1β and TNF-α levels were lower compared with those of LPS stimulation, and IRAK-4 siRNA could suppress production of pro-inflammatory cytokines. These findings suggest that besides IRAK-M, IRAK-4 also plays an important role in the local inflammatory reaction and contributes to prosthesis loosening.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"470-482"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17534259211018740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39241955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Acanthopanax senticosus supplementation on innate immunity and changes of related immune factors in healthy mice. 补充刺五加对健康小鼠先天免疫的影响及相关免疫因子的变化。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2020-09-16 DOI: 10.1177/1753425920955200

YunQiang Zhang, YunLu Zhang, ZiKui Liu

{"title":"Effects of Acanthopanax senticosus supplementation on innate immunity and changes of related immune factors in healthy mice.","authors":"YunQiang Zhang, YunLu Zhang, ZiKui Liu","doi":"10.1177/1753425920955200","DOIUrl":"https://doi.org/10.1177/1753425920955200","url":null,"abstract":"Modern scientific research has shown that Acanthopanax senticosus (AS) can regulate the innate immunity of healthy animals, thus affecting the health of animals. However, there are few systematic reports on the changes of innate immune indices of healthy animals after consuming AS. The purpose of this project was to study the effect on healthy mice's innate immunity and changes of related immune factors induced by feeding AS root powder supplementation. The results showed that the killing rate of natural cells increased in a dose-dependent manner in a certain time period. Compared to the control group, the treatment groups (T1, T2 and T3) improved significantly in the innate immune index (lysozyme, β-defensin-2 and duodenal secretory IgA (SIgA) to varying degrees) and induced corresponding changes of immune factors at certain time periods. The correlation between SIgA and IFN-γ in mouse serum was enhanced, and the higher the concentration of AS in the diet, the stronger the correlation was. However, there was no significant difference in growth performance among groups. It is proved that AS supplementation can enhance innate immunity and change several relevant immune factors and cells of healthy mice without affecting growth performance.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"461-469"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920955200","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38386762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation. 沃尔夫-赫希霍恩综合征候选病例 1 通过 NEK7 介导的 NLRP3 炎性体激活，在脓毒症诱发的急性肺损伤中促进肺泡巨噬细胞的脓毒症。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-08-25 DOI: 10.1177/17534259211035426

Caixia Liu, Benlong Cai, Dan Li, Yuan Yao

{"title":"Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.","authors":"Caixia Liu, Benlong Cai, Dan Li, Yuan Yao","doi":"10.1177/17534259211035426","DOIUrl":"10.1177/17534259211035426","url":null,"abstract":"Sepsis is a complex clinical syndrome with high incidence and mortality. Acute lung injury (ALI) is a common complication of sepsis. At present, there is no effective therapeutic strategy to treat ALI. The SET domain-containing histone methyltransferase Wolf-Hirschhorn syndrome candidate 1 (WHSC1) regulates cancer progression, while its role in sepsis-induced ALI remains unclear. Thus, this study aimed to study the effect of WHSC1 on sepsis-induced ALI and to explore the potential mechanism of action. In the study, LPS treatment induced lung injury. WHSC1 was highly expressed in LPS-induced ALI. Knockdown of WHSC1 attenuated LPS-induced ALI and pyroptosis in vivo. Besides, knockdown of WHSC1 attenuated LPS-induced alveolar macrophage pyroptosis in vitro. Furthermore, NIMA-related kinase-7 (NEK7) expression could be regulated by WHSC1, and NEK7 bound to NLRP3 in alveolar macrophages. Moreover, WHSC1 regulated alveolar macrophage pyroptosis through modulating NEK7-mediated NLRP3 inflammasome activation. In conclusion, WHSC1 was highly expressed in LPS-induced ALI. WHSC1 facilitated alveolar macrophage pyroptosis in sepsis-induced ALI through NEK7-mediated NLRP3 inflammasome activation. WHSC1 may be a valuable target for the therapy of sepsis-induced ALI.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"437-447"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/af/10.1177_17534259211035426.PMC8504266.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39340831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neglected roles of IgG Fc-binding protein secreted from airway mucin-producing cells in protecting against SARS-CoV-2 infection. 气道粘液生成细胞分泌的IgG fc结合蛋白在预防SARS-CoV-2感染中的作用被忽视。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-09-15 DOI: 10.1177/17534259211043159

Kensuke Kobayashi, Mitsuhiro Tachibana, Yutaka Tsutsumi

{"title":"Neglected roles of IgG Fc-binding protein secreted from airway mucin-producing cells in protecting against SARS-CoV-2 infection.","authors":"Kensuke Kobayashi, Mitsuhiro Tachibana, Yutaka Tsutsumi","doi":"10.1177/17534259211043159","DOIUrl":"https://doi.org/10.1177/17534259211043159","url":null,"abstract":"Both innate immunity and acquired immunity are involved in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The induction of Abs that neutralize the virus has been described, and certain Abs against endemic coronaviruses may cross-react with SARS-CoV-2. Detailed mechanisms to protect against the pandemic of SARS-CoV-2 remain unresolved. We previously reported that IgG Fc-binding protein (Fcγbp), a unique, large molecular weight, and mucin-like secretory Fc receptor protein, secreted from goblet cells of human small and large intestine, mediates the transportation of serum IgG onto the mucosal surface. In this review, we show that mucous bronchial gland cells and some goblet cells are immunoreactive for Fcγbp. Fcγbp traps the cross-reactive (both neutralizing and non-neutralizing) IgG bound to the virus and can consequently eliminate the virus from the mucosal surface to decrease viral loads. Fcγbp can also suppress immune overreaction by interfering with Fc-binding by macrophages and competing with complement fixation. Fcγbp secreted from mucin-producing cells of the airway functions as an important anti-infection mucosal defense. The Fcγbp-mediated mechanism can be a key factor in explaining why SARS-CoV-2 is less infective/lethal in children, and may also be involved in the unique Ab response, recurrent infection, and effects of serum therapy and vaccination.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"423-436"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/90/10.1177_17534259211043159.PMC8504265.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39415547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Differential induction of innate memory in porcine monocytes by β-glucan or bacillus Calmette-Guerin. β-葡聚糖和卡介苗芽孢杆菌对猪单核细胞先天记忆的差异诱导。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2020-08-30 DOI: 10.1177/1753425920951607

Kristen A Byrne, Christopher K Tuggle, Crystal L Loving

{"title":"Differential induction of innate memory in porcine monocytes by β-glucan or bacillus Calmette-Guerin.","authors":"Kristen A Byrne, Christopher K Tuggle, Crystal L Loving","doi":"10.1177/1753425920951607","DOIUrl":"https://doi.org/10.1177/1753425920951607","url":null,"abstract":"Innate immunomodulation via induction of innate memory is one mechanism to alter the host's innate immune response to reduce or prevent disease. Microbial products modulate innate responses with immediate and lasting effects. Innate memory is characterized by enhanced (training) or depressed (tolerance) innate immune responses, including pro-inflammatory cytokine production, to secondary exposure following a priming event. To investigate the ability of β-glucans and bacillus Calmette-Guerin to induce innate training or tolerance in pig cells, porcine monocytes were cultured with priming agonist (β-glucans or bacillus Calmette-Guerin) then re-stimulated 5 d later with a heterologous microbial agonist to determine induction of innate memory. Priming with β-glucan from Saccharomyces cerevisiae depressed IL-1β and TNF-α cytokine responses to re-stimulation with LPS, indicative of a tolerized state. However, bacillus Calmette-Guerin priming induced a trained state in porcine monocytes, as LPS re-stimulation enhanced IL-1β and TNF-α gene expression and protein production. We present the first evidence of innate memory in pig monocytes, with bacillus Calmette-Guerin (training) or Saccharomyces cerevisiae β-glucan (tolerance). Induction of a trained or tolerized state in vitro is a first step to identify agonists to alter the innate immune system at the animal level with the intent of enhancing disease resistance.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"448-460"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920951607","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38419990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis. TLR4和TLR9多态性:在比哈尔邦印度内脏利什曼病人群中易感性的可能作用

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-08-01 Epub Date: 2021-04-28 DOI: 10.1177/1753425920965658

Abhishek Mandal, Manish Kumar, Ashish Kumar, Abhik Sen, Pradeep Das, Sushmita Das

{"title":"TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis.","authors":"Abhishek Mandal, Manish Kumar, Ashish Kumar, Abhik Sen, Pradeep Das, Sushmita Das","doi":"10.1177/1753425920965658","DOIUrl":"https://doi.org/10.1177/1753425920965658","url":null,"abstract":"Genetic variations in the host TLRs genes play an important role in susceptibility and/or resistance to visceral leishmaniasis by altering the host-pathogen interaction. In this study, we investigated the association between polymorphisms of TLR4 (Asp299Gly, Thr399Ile) and TLR-9 (T-1237C), with susceptibility to visceral leishmaniasis. A bi-directional PCR amplification of specific alleles technique was used to characterize the distribution of TLR4 (Asp299Gly and Thr399Ile) and TLR9 (T-1237C) polymorphisms. A total of 60 samples were randomly selected from confirmed visceral leishmaniasis patients and 24 endemic healthy volunteers. The samples were genotyped and allele frequencies were determined. We observed that TLR4 Asp299Gly and Thr399Ile genotypes were more frequent in visceral leishmaniasis patients (10% and 15% respectively) compared to controls (4.2% and 8.3% respectively). However, the differences were not significant in TLR4 Asp299Gly and Thr399Ile alleles and genotypes. In the case of TLR9, we observed the frequency of T1237C genotype was higher in visceral leishmaniasis patients (43.3%) than in healthy controls (33.3%). Statistically significant differences were observed in TLR9 T1237C alleles and genotypes. We concluded that TLR9 T1237C, but not TLR4, gene polymorphisms can be regarded as contributors to visceral leishmaniasis susceptibility among the Indian population of Bihar state.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 6","pages":"493-500"},"PeriodicalIF":3.2,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1753425920965658","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38917749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver? 当通过肝脏的第一次或第二次LPS刺激引发急性期反应时，慢性饮食暴露于真菌毒素脱氧雪腐镰刀菌醇是否会影响猪肝脏转录组?

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-07-01 Epub Date: 2021-07-31 DOI: 10.1177/17534259211030563

Sven Dänicke, Ann-Katrin Heymann, Michael Oster, Klaus Wimmers, Tanja Tesch, Erik Bannert, Susanne Bühler, Susanne Kersten, Jana Frahm, Jeannette Kluess, Stefan Kahlert, Hermann-Josef Rothkötter, Fabian Billenkamp

{"title":"Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver?","authors":"Sven Dänicke, Ann-Katrin Heymann, Michael Oster, Klaus Wimmers, Tanja Tesch, Erik Bannert, Susanne Bühler, Susanne Kersten, Jana Frahm, Jeannette Kluess, Stefan Kahlert, Hermann-Josef Rothkötter, Fabian Billenkamp","doi":"10.1177/17534259211030563","DOIUrl":"https://doi.org/10.1177/17534259211030563","url":null,"abstract":"The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammation might play an additional role. First and second-pass exposure of the liver with LPS for triggering a SI was achieved by LPS infusion via pre- or post-hepatic venous route, respectively. Each infusion group was pre-conditioned either with a control diet (0.12 mg DON/kg diet) or with a DON-contaminated diet (4.59 mg DON/kg diet) for 4 wk. Liver transcriptome was evaluated at 195 min after starting infusions. DON exposure alone failed to modulate the mRNA expression significantly. However, pre- and post-hepatic LPS challenges prompted transcriptional responses in immune and metabolic levels. The mRNAs for B-cell lymphoma 2-like protein 11 as a key factor in apoptosis and IFN-γ released by T cells were clearly up-regulated in DON-fed group infused with LPS post-hepatically. On the other hand, mRNAs for nucleotide binding oligomerization domain containing 2, IFN-α and eukaryotic translation initiation factor 2α kinase 3 as ribosomal stress sensors were exclusively up-regulated in control pigs with pre-hepatic LPS infusion. These diverse effects were traced back to differences in TLR4 signalling.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 5","pages":"388-408"},"PeriodicalIF":3.2,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/3b/10.1177_17534259211030563.PMC8419296.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39267456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of genetic polymorphisms in TLR6 and TLR10 genes on the risk of pulmonary tuberculosis in a Moldavian population. TLR6和TLR10基因遗传多态性对摩尔多瓦人群肺结核风险的协同作用

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-07-01 Epub Date: 2021-07-18 DOI: 10.1177/17534259211029996

Alexander Varzari, Igor V Deyneko, Elena Tudor, Harald Grallert, Thomas Illig

{"title":"Synergistic effect of genetic polymorphisms in TLR6 and TLR10 genes on the risk of pulmonary tuberculosis in a Moldavian population.","authors":"Alexander Varzari, Igor V Deyneko, Elena Tudor, Harald Grallert, Thomas Illig","doi":"10.1177/17534259211029996","DOIUrl":"https://doi.org/10.1177/17534259211029996","url":null,"abstract":"Polymorphisms in genes that control immune function and regulation may influence susceptibility to pulmonary tuberculosis (TB). In this study, 14 polymorphisms in 12 key genes involved in the immune response (VDR, MR1, TLR1, TLR2, TLR10, SLC11A1, IL1B, IL10, IFNG, TNF, IRAK1, and FOXP3) were tested for their association with pulmonary TB in 271 patients with TB and 251 community-matched controls from the Republic of Moldova. In addition, gene-gene interactions involved in TB susceptibility were analyzed for a total of 43 genetic loci. Single nucleotide polymorphism (SNP) analysis revealed a nominal association between TNF rs1800629 and pulmonary TB (Fisher exact test P = 0.01843). In the pairwise interaction analysis, the combination of the genotypes TLR6 rs5743810 GA and TLR10 rs11096957 GT was significantly associated with an increased genetic risk of pulmonary TB (OR = 2.48, 95% CI = 1.62-3.85; Fisher exact test P value = 1.5 × 10-5, significant after Bonferroni correction). In conclusion, the TLR6 rs5743810 and TLR10 rs11096957 two-locus interaction confers a significantly higher risk for pulmonary TB; due to its high frequency in the population, this SNP combination may serve as a novel biomarker for predicting TB susceptibility.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 5","pages":"365-376"},"PeriodicalIF":3.2,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17534259211029996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39195722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Long non-coding RNA ZFAS1 alleviates sepsis-induced myocardial injury via target miR-34b-5p/SIRT1. 长链非编码RNA ZFAS1通过靶miR-34b-5p/SIRT1减轻败血症诱导的心肌损伤。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-07-01 Epub Date: 2021-08-02 DOI: 10.1177/17534259211034221

Dan-Dan Chen, Hong-Wu Wang, Xing-Jun Cai

{"title":"Long non-coding RNA ZFAS1 alleviates sepsis-induced myocardial injury via target miR-34b-5p/SIRT1.","authors":"Dan-Dan Chen, Hong-Wu Wang, Xing-Jun Cai","doi":"10.1177/17534259211034221","DOIUrl":"https://doi.org/10.1177/17534259211034221","url":null,"abstract":"Long non-coding RNA ZFAS1 is down-regulated in sepsis. However, whether ZFAS1 participates in sepsis-induced cardiomyopathy (SIC) remains largely unknown. LPS injection to rats was used to establish an in vivo sepsis model, while LPS stimulation with H9C2 cell was used to mimic an in vitro sepsis-induced myocardial injury model. Western blots and quantitative RT-PCR were performed to evaluate protein and mRNA levels, respectively. ELISA was conducted to determine cytokine levels in supernatant. Flow cytometry was used to test apoptosis. Dual-luciferase assay was performed to validate binding between ZFAS1 and miR-34b-5p, miR-34b-5p and SIRT1. Our data revealed that ZFAS1 and SIRT1 were down-regulated, while miR-34b-5p was up-regulated in LPS-induced H9C2 cells. Inhibition of miR-34b-5p or overexpression of ZFAS1 alleviated inflammatory response and cell apoptosis in LPS-stimulated H9C2 cells. A mechanism study revealed that ZFAS1 sponged miR-34b-5p and thus elevated expression of SIRT1, which was prohibited by miR-34b-5p. ZFAS1 alleviated inflammatory response and cell apoptosis in LPS-stimulated H9C2 cells via the miR-34b-5p/SIRT1 axis, providing novel potential therapeutic targets for SIC.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 5","pages":"377-387"},"PeriodicalIF":3.2,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17534259211034221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39269232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of fatty acid nitroalkanes on signal transduction pathways and airway macrophage activation. 脂肪酸硝基烷烃对信号转导通路和气道巨噬细胞活化的影响。

IF 3.2 4区医学

Innate Immunity Pub Date : 2021-07-01 Epub Date: 2021-08-10 DOI: 10.1177/17534259211015330

Melissa L Wilkinson, Andrew J Gow

{"title":"Effects of fatty acid nitroalkanes on signal transduction pathways and airway macrophage activation.","authors":"Melissa L Wilkinson, Andrew J Gow","doi":"10.1177/17534259211015330","DOIUrl":"10.1177/17534259211015330","url":null,"abstract":"Fatty acid nitroalkenes are reversibly-reactive electrophiles that are endogenously detectable at nM concentrations and display anti-inflammatory, pro-survival actions. These actions are elicited through the alteration of signal transduction proteins via a Michael addition on nucleophilic cysteine thiols. Nitrated fatty acids (NO2-FAs), like 9- or 10-nitro-octadec-9-enolic acid, will act on signal transduction proteins directly or on key regulatory proteins to cause an up-regulation or down-regulation of the protein's expression, yielding an anti-inflammatory response. These responses have been characterized in many organ systems, such as the cardiovascular system, with the pulmonary system less well defined. Macrophages are one of the most abundant immune cells in the lung and are essential in maintaining lung homeostasis. Despite this, macrophages can play a role in both acute and chronic lung injury due to up-regulation of anti-inflammatory signal transduction pathways and down-regulation of pro-inflammatory pathways. Through their propensity to alter signal transduction pathways, NO2-FAs may be able to reduce macrophage activation during pulmonary injury. This review will focus on the implications of NO2-FAs on macrophage activation in the lung and the signal transduction pathways that may be altered, leading to reduced pulmonary injury.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"27 5","pages":"353-364"},"PeriodicalIF":3.2,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fa/17/10.1177_17534259211015330.PMC8419298.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39297143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1