Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells.

IF 2.8 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Innate Immunity Pub Date : 2021-10-01 Epub Date: 2021-11-18 DOI:10.1177/17534259211057038

Di Zhao, Bo Yang, Chen Ye, Shaoyi Zhang, Xiaoqiong Lv, Qiyi Chen

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引用次数: 3

Abstract

Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease.

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肠内营养通过先天淋巴样细胞激活特殊的促溶解介质，改善小鼠克罗恩病的症状。

克罗恩病激活炎症反应，诱发肠道紊乱。肠内营养可发挥全身免疫调节作用。采用盲肠结扎穿孔术(CLP)建立克罗恩病小鼠模型。采用生存分析、苏木精-伊红染色、流式细胞术、ELISA、Western blot和液相色谱-串联质谱。加入黄芩素抑制脂氧合酶。与CLP治疗相比，EN治疗可恢复大鼠的存活率，改善炎症损伤、腹腔灌洗液中渗出的中性粒细胞和血清中IL-6、TNF-α的水平。EN可提高腹腔灌洗物中ILC-3含量、5/15-LOX水平及RvD1-RvD5水平。除ILC-3含量外，黄芩苷逆转了EN的所有检测效果。EN可通过ILCs激活特殊促溶解介质(SPMs)，减轻克罗恩病的损伤。

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来源期刊

Innate Immunity 生物-免疫学

CiteScore

7.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Innate Immunity is a highly ranked, peer-reviewed scholarly journal and is the official journal of the International Endotoxin & Innate Immunity Society (IEIIS). The journal welcomes manuscripts from researchers actively working on all aspects of innate immunity including biologically active bacterial, viral, fungal, parasitic, and plant components, as well as relevant cells, their receptors, signaling pathways, and induced mediators. The aim of the Journal is to provide a single, interdisciplinary forum for the dissemination of new information on innate immunity in humans, animals, and plants to researchers. The Journal creates a vehicle for the publication of articles encompassing all areas of research, basic, applied, and clinical. The subject areas of interest include, but are not limited to, research in biochemistry, biophysics, cell biology, chemistry, clinical medicine, immunology, infectious disease, microbiology, molecular biology, and pharmacology.