Innate Immunity最新文献

{"title":"Circulating MiR-126 as a potential biomarker in Egyptian colorectal cancer patients: A case-control study.","authors":"Rasha Ahmed Ghorab, Shaimaa H Fouad, Ahmed F Sherief, Rana M Taha, Marwa Hamdy, Mohammad M Darwish, Eman M El-Sehsah, Sara I Taha","doi":"10.1177/17534259241308661","DOIUrl":"10.1177/17534259241308661","url":null,"abstract":"<p><strong>Background: </strong>Globally, colorectal cancer (CRC) is among the most prevalent malignant tumors. It is characterized by unlimited proliferation, invasion, and metastasis. MicroRNA-126 (miR-126) has been shown in many studies to play a significant role in CRC, but data regarding its role in CRC Egyptian patients are limited.</p><p><strong>Objectives: </strong>This case-control study aimed to investigate the miR-126 as a potential marker in CRC Egyptian patients and to correlate its expression levels with CRC tumor, node, metastasis (TNM) stage, distant metastasis, and tumor size.</p><p><strong>Methods: </strong>The study included 50 adult Egyptian participants (30 patients with CRC, 10 patients with colorectal adenoma as a pathological control, and 10 healthy controls). MiR-126 expression levels were detected using Real-Time Quantitative PCR (qPCR) along with the endogenous reference gene hsa-miR-103a in all participants.</p><p><strong>Results: </strong>MiR-126 expression was significantly decreased in CRC patients than both control groups. It was associated with advanced TNM stage (<i>p </i>= 0.001) and distant metastasis (<i>p </i>= 0.002). However, it was not correlated with tumor size (<i>p </i>= 0.980), carcinoembryonic antigen (CEA) (<i>p </i>= 0.397), and cancer antigen 19-9 (CA19-9) (<i>p </i>= 0.236). The best cut-off point of miR-126 to discriminate CRC from both controls was 0.7 and to discriminate metastatic CRC from non-metastatic CRC was 0.3.</p><p><strong>Conclusions: </strong>Our results suggest that miR-126 could be used as an early marker for CRC detection among Egyptian patients and a good prognostic indicator associated with metastasis.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"17534259241308661"},"PeriodicalIF":2.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective IgG binding to the LPS glycolipid core found in bovine colostrum, or milk, during Escherichia coli mastitis influences endotoxin function","authors":"Suzanne M. Hurst, David A. L. Flossdorf, Raveen Koralagamage Don, Anton Pernthaner","doi":"10.1177/17534259241269724","DOIUrl":"https://doi.org/10.1177/17534259241269724","url":null,"abstract":"The dynamic interplay between intramammary IgG, formation of antigen-IgG complexes and effector immune cell function is essential for immune homeostasis within the bovine mammary gland. We explore how changes in the recognition and binding of anti-LPS IgG to the glycolipid “functional” core in milk from healthy or clinically diagnosed Escherichia coli (E. coli) mastitis cows’ controls endotoxin function. In colostrum, we found a varied anti-LPS IgG repertoire and novel soluble LPS/IgG complexes with direct IgG binding to the LPS glycolipid core. These soluble complexes, absent in milk from healthy lactating cows, were evident in cows diagnosed with E. coli mastitis and correlated with endotoxin-driven inflammation. E. coli mastitis milk displayed a proportional reduction in anti-LPS glycolipid core IgG compared to colostrum. Milk IgG extracts showed that only colostrum IgG attenuated LPS induced endotoxin activity. Furthermore, LPS-stimulated reactive oxygen species (ROS) in milk granulocytes was only suppressed by colostrum IgG, while IgG extracts of neither colostrum nor E. coli mastitis milk influenced N-formylmethionine-leucyl-phenylalanine (fMLP)-stimulated ROS in LPS primed granulocytes. Our findings support bovine intramammary IgG diversity in health and in response to E. coli infection generate milk anti-LPS IgG repertoires that coordinate appropriate LPS innate-adaptive immune responses essential for animal health.","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":"8 1","pages":"96-118"},"PeriodicalIF":3.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory bioenergetics is enhanced in human, but not bovine macrophages after exposure to <i>M. bovis</i> PPD: Exploratory insights into overall similar Cellular Metabolic Profiles.","authors":"Marie-Christine Bartens, Sam Willcocks, Dirk Werling, Amanda J Gibson","doi":"10.1177/17534259241296630","DOIUrl":"10.1177/17534259241296630","url":null,"abstract":"<p><p>The role of macrophage (MØ) cellular metabolism and reprogramming during TB infection is of great interest due to the influence of <i>Mycobacterium</i> spp. on MØ bioenergetics. Recent studies have shown that <i>M. tuberculosis</i> induces a TLR2-dependent shift towards aerobic glycolysis, comparable to the established LPS induced pro-inflammatory M1 MØ polarisation. Distinct differences in the metabolic profile of murine and human MØ indicates species-specific differences in bioenergetics. So far, studies examining the metabolic potential of bovine MØ are lacking, thus the basic bioenergetics of bovine and human MØ were explored in response to a variety of innate immune stimuli. Cellular energy metabolism kinetics were measured concurrently for both species on a Seahorse XFe96 platform to generate bioenergetic profiles for the response to the bona-fide TLR2 and TLR4 ligands, FSL-1 and LPS respectively. Despite previous reports of species-specific differences in TLR signalling and cytokine production between human and bovine MØ, we observed similar respiratory profiles for both species. Basal respiration remained constant between stimulated MØ and controls, whereas addition of TLR ligands induced increased glycolysis, as measured by the surrogate parameter ECAR. In contrast to MØ stimulation with <i>M. tuberculosis</i> PPD, another TLR2 ligand, <i>M. bovis</i> PPD treatment significantly enhanced basal respiration rates and glycolysis only in human MØ. Respiratory profiling further revealed significant elevation of ATP-linked OCR and maximal respiration suggesting a strong OXPHOS activation upon <i>M. bovis</i> PPD stimulation in human MØ. Our results provide an exploratory set of data elucidating the basic respiratory profile of bovine vs. human MØ that will not only lay the foundation for future studies to investigate host-tropism of the <i>M. tuberculosis</i> complex but may explain inflammatory differences observed for other zoonotic diseases.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"136-149"},"PeriodicalIF":2.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11577332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innate lymphoid cells and infectious diseases.","authors":"Ting Yuan, Qianhui Zhou, Yuqiu Tian, Yangjing Ou, YunZhu Long, YingZheng Tan","doi":"10.1177/17534259241287311","DOIUrl":"10.1177/17534259241287311","url":null,"abstract":"<p><p>Innate lymphoid cells (ILCs) are the main resident lymphocytes that mostly reside in tissues owing to the lack of adaptive antigen receptors. These cells are involved in early anti-infective immunity, antitumour immunity, regulation of tissue inflammation, and maintenance of homeostasis in the internal environment of tissues and have been referred to as the \"first armies stationed in the human body\". ILCs are widely distributed in the lungs, colon, lymph nodes, oral mucosa and even embryonic tissues. Due to the advantage of their distribution location, they are often among the first cells to come into contact with pathogens.Relevant studies have demonstrated that ILCs play an early role in the defence against a variety of pathogenic microorganisms, including bacteria, viruses, fungi and helminths, before they intervene in the adaptive immune system. ILCs can initiate a rapid, nonspecific response against pathogens prior to the initiation of an adaptive immune response and can generate a protective immune response against specific pathogens, secreting different effectors to play a role.There is growing evidence that ILCs play an important role in host control of infectious diseases. In this paper, we summarize and discuss the current known infectious diseases in which ILCs are involved and ILC contribution to the defence against infectious diseases. Further insights into the mechanisms of ILCs action in different infectious diseases will be useful in facilitating the development of therapeutic strategies for early control of infections.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"120-135"},"PeriodicalIF":2.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study of monocytes in relapsing remitting multiple sclerosis: Correlation with disease activity.","authors":"Sara I Taha, Hala Ghareeb Mohamed, Rasha Mamdouh, Nada E Kamal, Shaimaa Sayed Khater","doi":"10.1177/17534259241269674","DOIUrl":"10.1177/17534259241269674","url":null,"abstract":"<p><p><b>Background:</b> Numerous immune cells are involved in developing multiple sclerosis (MS). Monocytes are believed to be the first to enter the brain and initiate inflammation. The role of monocyte subtypes in MS needs to be better understood. Objective: The current study aims to investigate the presence of different subsets of monocytes in relapsing-remitting MS (RRMS) Egyptian patients and their correlation with disease activity. <b>Methods:</b> This study included 44 RRMS patients (22 patients in relapse, 22 patients in remission), diagnosed according to the 2017 MacDonalds criteria, and 44 matched healthy controls. Personal and medical histories were taken from the patients, and the Expanded Disability Status Scale (EDSS) was used to evaluate the degree of impairment. Characterization of peripheral blood monocyte subsets was done by flow cytometry for all participants. <b>Results:</b> The percentage of classical, intermediate, and non-classical monocyte subsets showed a significant increase in RRMS patients than controls with <i>p</i>-values of 0.029, 0.049, and 0.043, respectively. In the RRMS patients, there were no statistically significant correlations (<i>p</i>-values >0.05) between the EDSS scores, the duration of disease, and number of relapses in the past year and the percentages of the various monocyte subsets. Furthermore, there were no significant differences in the percentage of each monocyte subset between RRMS patients in remission and those experiencing a relapse (<i>p</i>-values >0.05). However, patients with evidence of activity in magnetic resonance imaging (MRI) had a significantly high percentage of non-classical monocytes with a <i>p</i>-value of 0.002. <b>Conclusion:</b> In RRMS patients, the three monocyte subsets (classical, non-classical and intermediate) increase significantly regardless of the disease activity. This increase denotes the vital role of monocytes and innate immunity in MS pathology, especially the non-classical monocyte subset. These findings suggest that monocytes might be a promising MS therapeutic target.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"90-95"},"PeriodicalIF":2.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The <i>in vitro</i> effect of myeloperoxidase oxidized LDL on THP-1 derived macrophages.","authors":"Elias Jeradeh, Christian Frangie, Samer Bazzi, Jalil Daher","doi":"10.1177/17534259241269687","DOIUrl":"10.1177/17534259241269687","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs) linked to atherosclerosis remains the leading cause of death worldwide. Atherosclerosis is primarily caused by the accumulation of oxidized forms of low density lipoprotein (LDL) in macrophages (MΦs) in the subendothelial layer of arteries leading to foam cell and fatty streak formation. Many studies suggest that LDL that is modified by myeloperoxidase (MPO) is a key player in the development of atherosclerosis. MΦs can adopt a variety of functional phenotypes that include mainly the proinflammatory M1 and the anti-inflammatory M2 MΦ phenotypes which are both implicated in the process of atherogenesis. In fact, MΦs that reside in atherosclerostic lesions were shown to express a variety of phenotypes ranging between the M1- and M2 MΦ types. Recently, we pointed out the involvement of MPO oxidized-LDL (Mox-LDL) in increasing inflammation in MΦs by reducing their secretion of IL-10. Since little is known about Mox-LDL-mediated pro-atherosclerostic responses in MΦs, our study aimed at analyzing the <i>in vitro</i> effects of Mox-LDL at this level through making use of the well-established model of human THP-1-derived Mφs. Our results demonstrate that Mox-LDL has no effect on apoptosis, reactive oxygen species (ROS) generation and cell death in our cell model; yet, interestingly, our results show that Mox-LDL is significantly engulfed at a higher rate in the different MΦ subtypes supporting its key role in foam cell formation during the progression of the disease as well as previous data that were generated using another primary MΦ cell model of atherosclerosis.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"82-89"},"PeriodicalIF":2.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin reduces neutrophil extracellular trap release in a rat model of rheumatoid arthritis, which is associated with a decrease in disease severity.","authors":"Yiqin Shu, Rui Yang, Huijie Wen, Qiannan Dong, Zhiqi Chen, Yang Xiang, Hao Wu","doi":"10.1177/17534259241255439","DOIUrl":"10.1177/17534259241255439","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA) is a chronic disease characterized by joint inflammation and severe disability. However, there is a lack of safe and effective drugs for treating RA. In our previous study, we discovered that myricetin (MC) and celecoxib have a synergistic effect in the treatment of RA. We conducted in vitro and in vivo experiments to further investigate the effects and mechanisms of action of MC. Our findings demonstrated that MC treatment effectively reduced the release of neutrophil extracellular traps (NETs) and alleviated the inflammatory response in RA. Mechanistic studies showed that MC prevents the entry of PADI4 and MPO into the cell nucleus, thereby protecting DNA from decondensation. In a rat arthritis model, MC improved histological changes in ankle joints and suppressed NET-related signaling factors. In conclusion, MC protects the ankle joints against arthritis by inhibiting MPO and PADI4, thereby reducing NET release. The pharmacological mechanism of MC in RA involves the inhibition of NET release.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"66-78"},"PeriodicalIF":2.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice.","authors":"","doi":"10.1177/17534259231224147","DOIUrl":"10.1177/17534259231224147","url":null,"abstract":"","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"80"},"PeriodicalIF":3.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nasal mucosal fibroblasts produce IL-4 to induce Th2 response.","authors":"Xianhai Zeng, Juanjuan Li, Jiangqi Liu, Lihua Mo, Yu Liu, Aizhi Zhang, Pingchang Yang, Hui Kong","doi":"10.1177/17534259241254623","DOIUrl":"10.1177/17534259241254623","url":null,"abstract":"<p><p>Th2 polarization is essential for the pathogenesis of allergic rhinitis (AR). Th2 polarization's mechanism requires further understanding. IL-4 is the primary cytokine involved in Th2 response. Fibroblasts play a role in immune regulation. This study aims to elucidate the role of nasal mucosal fibroblast-derived IL-4 in the induction of Th2 responses. Nasal mucosal tissues were obtained from surgically removed samples from patients with nasal polyps, whether with or without AR. Fibroblasts were isolated from the tissues by flow cytometry cell sorting, and analyzed by RNA sequencing (RNAseq). The data from RNAseq showed that nasal fibroblasts expressed genes of <i>GATA3, CD80, CD83, CD86, STAT6, IL2, IL4, IL5, IL6, IL13</i> and costimulatory factor. The data were verified by RT-qPCR. The level of gene activity was positively correlated with those of AR-related cytokines present in nasal secretions. Nasal fibroblasts release IL-4 upon activation. Nasal fibroblasts had the ability to transform naive CD4⁺ T cells into Th2 cells, which can be eliminated by inhibiting IL-4 receptor or CD28 in CD4⁺ T cells. To sum up, nasal mucosal fibroblasts produce IL-4, which can induce Th2 cell development. The data implicate that nasal fibroblasts are involved in the pathogenesis of nasal allergy.</p>","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"55-65"},"PeriodicalIF":2.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice.","authors":"","doi":"10.1177/17534259231224146","DOIUrl":"10.1177/17534259231224146","url":null,"abstract":"","PeriodicalId":13676,"journal":{"name":"Innate Immunity","volume":" ","pages":"79"},"PeriodicalIF":3.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}