Indian Journal of Microbiology Research最新文献_第8页

Diagnostic utility of adenosine deaminase in bronchoalveolar lavage (BAL) in pulmonary tuberculosis from a tertiary care hospital in North India 印度北部三级医院支气管肺泡灌洗(BAL)中腺苷脱氨酶的诊断价值

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.038

Unairah Naqash, Rukhsana Taj, Nahid Nahvi

{"title":"Diagnostic utility of adenosine deaminase in bronchoalveolar lavage (BAL) in pulmonary tuberculosis from a tertiary care hospital in North India","authors":"Unairah Naqash, Rukhsana Taj, Nahid Nahvi","doi":"10.18231/j.ijmr.2022.038","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.038","url":null,"abstract":"Adenosine Deaminase (ADA) has been acclaimed as a biochemical marker in numerous studies in the diagnosis of tuberculosis of pleura, peritoneum, meninges etc because of its high sensitivity and specificity. As a diagnostic test, ADA offers several advantages; it is rapid, simple, low cost, non-invasive and can be performed easily in most clinical laboratories. The current retrospective study was carried out on a total of 91 specimens of bronchoalveolar lavage collected on bronchoscopy. The samples were collected and sediments were confirmed for presence of tubercular bacilli through Lowenstein Jensen(LJ) media (Gold standard), GeneXpert(CBNAAT).Simultaneously the ADA estimation was done from supernatant fluid obtained after centrifugation of sample.The Mean ADA level for Culture Positive samples in BAL was 5.899± 1.723 and from Culture Negative Samples the Mean ADA was1.217 ± 1.439. The ADA cut off levels of >4.0 IU/L in BAL when compared with LJ culture media (gold standard) showed a sensitivity of 90.0% and a specificity of 97.2%. Upon ROC analysis a high rate of accuracy was recorded in diagnosis of TB through ADA estimation with sensitivity and specificity reaching 100% and 97.2% at a ADA cut off 3.76 IU/L when compared with LJ culture media (gold standard). However, the sensitivity and specificity achieved was slightly lower when ADA results were compared with GeneXpert results.The determination of ADA levels may help in early diagnosis, improve the prognosis and reduce the spread of disease and thereby the test should be included in routine investigations in patients suspected of tuberculosis.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86205078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-viral activity of suramin against influenza A virus in A549 cells 苏拉明对A549细胞甲型流感病毒的抗病毒活性研究

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.034

M. Khanna, Tanusri Nandi

{"title":"Anti-viral activity of suramin against influenza A virus in A549 cells","authors":"M. Khanna, Tanusri Nandi","doi":"10.18231/j.ijmr.2022.034","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.034","url":null,"abstract":"Influenza A virus (IAV) is highly contagious global factor of causing pandemics and epidemics occasionally. Suramin, an anti-trypanosomal drug, also under evaluation as anti-cancer and anti-viral agent. However, inhibitory effect of suramin against influenza A virus have not yet been explored. The purpose of this study was to investigate the anti-viral activity of suramin against IAV. Cytotoxicity assay (IC) of suramin and oseltamivir was determined using MTT assay in A549 cells. The anti-viral effect of suramin was determined by RT-qPCR, western blot and CPE (Cytopathic effect). Oseltamivir, an antiviral drug that has established anti influenza activity was used as positive control drug. Half-maximal toxic concentration of suramin was found to be at 269.2 µg/ml (Log IC=2.43 µg/ml) and IC for oseltamivir was 145.4 µg/ml (Log IC=2.16 µg/ml) in A549 cells at 24hr. Suramin exhibited anti-viral activity at all the concentration, however at 250 µg/ml and 125 µg/ml demonstrated significant down regulation of viral RNA and protein in A549 cells. Oseltamivir, a positive control drug also showed significant viral inhibition at 200 and 100 µg/ml at 24hr. : Suramin is effective in inhibiting influenza A virus replication in A549 cells at lower concentration.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73370189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To study drug resistance & biofilm production in gram negative isolates from clinical samples 目的:研究临床革兰氏阴性分离菌的耐药性及生物膜的生成

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.036

P. Prakash, R. Agarwal, E. Gupta, R. Rathore, Vishakha Ashopa, Eshank Gupta

{"title":"To study drug resistance & biofilm production in gram negative isolates from clinical samples","authors":"P. Prakash, R. Agarwal, E. Gupta, R. Rathore, Vishakha Ashopa, Eshank Gupta","doi":"10.18231/j.ijmr.2022.036","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.036","url":null,"abstract":"Biofilm are groups of microorganism encased in a matrix of extracellular polysaccharide (slime), called polysaccharide intercellular adhesion (PIA). Bacteria commonly involved include , , , and . The present study was performed to identify antibiotic resistance pattern & their ability to form biofilm in gram negative clinical isolates. All clinical samples received in laboratory for microbial culture during study period of 12 months (2017 to 2018) were included in this study Antibiotic susceptibility testing, ESBL & MBL detection was done for clinical isolates. Biofilm productions were determined by Congo red agar method, Christenson’s Test Tube method and Tissue culture plate method. 327 gram negative isolates were detected. Maximum were (32.72%) followed by (28.44%), (16.51%), (16.51%), Citrobacter species (3.97%). Maximum isolates showed resistance to ampicillin (93.27%) followed by amoxiclave (87.46%), ceftazidime (74%). Out of 327 GNB isolates, biofilm produced by 64 (19.57%) isolates by Tissue culture plate (TCP) method, 38(11.62%) by Congo red agar (CRA) methods and 23 (7.03%) by Tube methods. Maximum biofilm were detected in (24.29%). There is increase prevalence of multidrug resistant& biofilm forming bacteria. The routine monitoring of multidrug resistance pattern & biofilm detection can be recommended in clinical laboratories to guide proper antibiotic treatment.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85419247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological importance of strigolactones hormone on arbuscular mycorrhizal fungi symbiosis in plant 独角甾内酯激素对植物丛枝菌根真菌共生的生态学意义

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.029

Debasis Mitra, A. Priyadarshini, A. Senapati, S. Behera, I. Chatterjee, P. Mohapatra, P. Panneerselvam

{"title":"Ecological importance of strigolactones hormone on arbuscular mycorrhizal fungi symbiosis in plant","authors":"Debasis Mitra, A. Priyadarshini, A. Senapati, S. Behera, I. Chatterjee, P. Mohapatra, P. Panneerselvam","doi":"10.18231/j.ijmr.2022.029","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.029","url":null,"abstract":"Strigolactones (SLs) are versatile compounds that have recently been identified as a special generation of plant hormones. They play a significant role as modulators of coordinated plant development in response to nutrient deficiency and defence, particularly by influencing plant root microbiome and mycorrhization. SLs act as signals molecules that help host communicate with their environment belowground, in addition to regulating root architecture and growth promotion. Alternatively, boosting the SLs hormone level or applying external SLs, SL synthetic analogs e.g. GR24, and SL mimics to plants, can improve the root architecture, and physiological changes, and controls biotic and abiotic parameters by activating regulatory genes and molecular changes. Interestingly, SLs perform a fundamental character in the establishment of arbuscular mycorrhiza fungi (AMF) symbiosis by eliciting mycorrhization in the plant, which allows for adequate phosphorus utilization. Due to various their multifunctional aspect, they have a wide range of possible agricultural and biotechnological applications. We should be able to comprehend the biological mechanisms operating below ground in plant systems and their significance in the ecosystem with greater clarity as more research is conducted into the necessary conditions for various SLs in various biological activities.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90230634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti herpes simplex virus-2 activity of the extracts of Sericostoma pauciflorum stocks ex wight and Lepidagathis trinervis Nees 少花丝绒和白头翁提取物抗单纯疱疹病毒2的活性研究

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.032

B. Pancholi

{"title":"Anti herpes simplex virus-2 activity of the extracts of Sericostoma pauciflorum stocks ex wight and Lepidagathis trinervis Nees","authors":"B. Pancholi","doi":"10.18231/j.ijmr.2022.032","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.032","url":null,"abstract":"To determine the anti-HSV 2 properties and mechanism of action of two important medicinal plants Stocks ex Wight (Boraginaceae) and Nees (Acanthaceae) To identify natural products with anti-HSV-2 activity, the aqueous and 50% ethanolic extracts were prepared from whole plants of and . Cytotoxicity assay using Vero cells as target revealed CC values of these extracts ranging from 301.8 to 436.8 µg ml Extracts from both the plants showed dose dependent inhibition of HSV-2 infection using Plaque Reduction Assay. Among the tested extracts, 50% ethanolic extract of showed lowest IC value of 6.86 µg ml. To study the possible mode of antiviral action, all the extracts showed 50 to 97% reduction in the formation of plaques in the attachment assay. Extracts prepared from also inhibited the HSV-2 penetration in a time and dose dependent manner. In post-attachment assay, both the extracts from as well as 50% ethanolic extract from showed significant inhibition in HSV-2 infection.These studies document anti-HSV-2 activity of the two commonly used medicinal plants that will have implications in the development of an alternate remedy for HSV-2 infection. Significant and impact of the study: This work provides information on the anti HSV-2 properties of Indian medicinal plants that showed their significant inhibition of HSV-2 virus in system.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91118542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological hazard identification in selective food products and their association with food safety practices in Hyderabad, India 在印度海得拉巴，选择性食品中的微生物危害鉴定及其与食品安全实践的关系

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.040

N. Kumar R, Uday Kumar P, Bhaskar V, Sudershan R V, K. Polasa, Hemalatha R, Sudip Ghosh

{"title":"Microbiological hazard identification in selective food products and their association with food safety practices in Hyderabad, India","authors":"N. Kumar R, Uday Kumar P, Bhaskar V, Sudershan R V, K. Polasa, Hemalatha R, Sudip Ghosh","doi":"10.18231/j.ijmr.2022.040","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.040","url":null,"abstract":"Microbiological risk assessment (MRA) is an emerging tool for evaluating the safety of food and water supplies. In this study, identification of microbial hazards in selected samples and their association with food safety practices were seen. Data were analyzed using Analysis of Variance (ANOVA) through the General Linear Models (GLM) procedure of the statistical analysis system software (SPSS version-11.5, 2003). The least significant differences were used to test means at p<0.05. Odds ratio (OR) values with 95% CI were computed to obtain the risk of the presence of the foodborne pathogen in a particular food. Analysis of 600 food samples indicated that (50%) was high in raw chicken samples followed by carrot salad (41%). The other emerging foodborne pathogens like (MRSA), , and were not detected in any of the food samples. Pathogens like (73.5%) in khoa, (45%) and (62.7%) in paneer were detected. A total of (n=143) cultures were analyzed for enterotoxin and coagulase enzyme. Nine cultures (6.3%) showed a positive result for enterotoxin production. For the risk assessment of contamination in foods, coagulase test and toxin production of isolates have to be evaluated. A significant association was found between the type of storage and log concentration of in khoa, whereas, with water for washing hands, the status of nails, and cleaning cloth were contributing to foodborne pathogens in other products. There is a need to provide food safety training to food handlers to improve food safety.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75738862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing awareness and execution of “Bio medical waste management rules 2016” among health care providers and grade 4 workers in a tertiary care hospital of Bhubaneswar 评估布巴内斯瓦尔一家三级保健医院的保健提供者和四级工作人员对" 2016年生物医疗废物管理规则"的认识和执行情况

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.035

Snigdha Singh, Nishant Panda, Imran Wasfi

{"title":"Assessing awareness and execution of “Bio medical waste management rules 2016” among health care providers and grade 4 workers in a tertiary care hospital of Bhubaneswar","authors":"Snigdha Singh, Nishant Panda, Imran Wasfi","doi":"10.18231/j.ijmr.2022.035","DOIUrl":"https://doi.org/10.18231/j.ijmr.2022.035","url":null,"abstract":"Hospital waste has always been a concern for the society. Recently the government has changed the BMW handling rules in 2016. However, mere introduction of laws is not sufficient for proper disposal of BMW. Awareness of these laws is also essential. Hence, this study was conducted with the objectives to assess the Knowledge, awareness, attitude & practice (KAP) towards biomedical waste management in Health care providers (HCP) and prevalence of needle-stick injury (NSI) in them.This was a cross-sectional study done from August 2018 to January 2019 in KIMS among 400 participants. A pre-tested, close ended questionnaire was used with few added questions related to new rules. An overall average level of KAP among HCP and grade 4 workers regarding BMW Rules and management 2016 with 86.49% doctors, 91.04% dentists, 79.55% nurses and 80% grade 4 workers securing average marks in the assessment. Moreover, 14 (20 .59%) doctors, 10 (14 .71%) dentists, 30 (44 .12%) nurses and 14 (20.59%) grade 4 workers reported that they had experienced NSI in the past 12 months. Overall prevalence of NSI is found to be 17%. We found an average level of knowledge, attitude and practice among HCP and grade 4 workers regarding BMW management. It is recommended that regular monitoring and training are required at all levels of Health Care System and the rules should be sincerely followed and practiced.","PeriodicalId":13428,"journal":{"name":"Indian Journal of Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75864130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical screening and antibacterial activity of Trachyspermum ammi L. against certain bacterial species 植物化学筛选及对某些细菌的抑菌活性研究

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.039

Aparajita Gupta, Mannu Kumari, Shabnam Sharma

引用次数: 0

Food fuel and fermentation products 食品燃料及发酵产品

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.030

Parthiban Meena, Arunchalam Manimekalan, Obebo Meshack Moranga

引用次数: 0

A review of maternal TORCH-S infections 母体TORCH-S感染的综述

Indian Journal of Microbiology Research Pub Date : 2022-10-15 DOI: 10.18231/j.ijmr.2022.031

P. Rajendiran, N. Saravanan, Mageshbabu Ramamurthy, Kumaran Vadivel, B. Nandagopal

引用次数: 0