To study drug resistance & biofilm production in gram negative isolates from clinical samples

P. Prakash, R. Agarwal, E. Gupta, R. Rathore, Vishakha Ashopa, Eshank Gupta
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Abstract

Biofilm are groups of microorganism encased in a matrix of extracellular polysaccharide (slime), called polysaccharide intercellular adhesion (PIA). Bacteria commonly involved include , , , and . The present study was performed to identify antibiotic resistance pattern & their ability to form biofilm in gram negative clinical isolates. All clinical samples received in laboratory for microbial culture during study period of 12 months (2017 to 2018) were included in this study Antibiotic susceptibility testing, ESBL & MBL detection was done for clinical isolates. Biofilm productions were determined by Congo red agar method, Christenson’s Test Tube method and Tissue culture plate method. 327 gram negative isolates were detected. Maximum were (32.72%) followed by (28.44%), (16.51%), (16.51%), Citrobacter species (3.97%). Maximum isolates showed resistance to ampicillin (93.27%) followed by amoxiclave (87.46%), ceftazidime (74%). Out of 327 GNB isolates, biofilm produced by 64 (19.57%) isolates by Tissue culture plate (TCP) method, 38(11.62%) by Congo red agar (CRA) methods and 23 (7.03%) by Tube methods. Maximum biofilm were detected in (24.29%). There is increase prevalence of multidrug resistant& biofilm forming bacteria. The routine monitoring of multidrug resistance pattern & biofilm detection can be recommended in clinical laboratories to guide proper antibiotic treatment.
目的:研究临床革兰氏阴性分离菌的耐药性及生物膜的生成
生物膜是包裹在细胞外多糖(黏液)基质中的微生物群,称为多糖细胞间粘附(PIA)。通常涉及的细菌包括、、和。本研究旨在鉴定革兰氏阴性临床分离株的抗生素耐药模式及其形成生物膜的能力。本研究纳入2017 - 2018年12个月期间实验室微生物培养的所有临床样本,进行抗生素药敏试验,对临床分离株进行ESBL和MBL检测。采用刚果红琼脂法、克里斯滕森试管法和组织培养板法测定生物膜产量。检出革兰氏阴性分离物327株。最多(32.72%),其次是(28.44%)、(16.51%)、(16.51%)和Citrobacter(3.97%)。对氨苄西林耐药最多(93.27%),其次是阿莫昔韦(87.46%)和头孢他啶(74%)。327株GNB分离株中,组织培养平板法(TCP) 64株(19.57%)、刚果红琼脂法(CRA) 38株(11.62%)、试管法23株(7.03%)产生生物膜。最大生物膜检出率为24.29%。多药耐药和形成生物膜的细菌的患病率增加。建议临床实验室开展常规多药耐药模式监测和生物膜检测,指导合理的抗生素治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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