Indian Journal of Pharmaceutical Education and Research最新文献_第9页

Evaluation of Cytotoxic Potential of Classical Siddha Medicine Padikara Parpam in Human Monocytic Leukemic Cell Lines (THP-1) 西达经典药物帕迪卡拉·帕帕对人单核细胞白血病细胞系（THP-1）细胞毒性的评估

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.72

Aswathy Karanath-Anilkumar, G. Munuswamy-Ramanujam, V. Soman, Shree Devi Munusamy Sampangiramulu, S. Parameswaran

{"title":"Evaluation of Cytotoxic Potential of Classical Siddha Medicine Padikara Parpam in Human Monocytic Leukemic Cell Lines (THP-1)","authors":"Aswathy Karanath-Anilkumar, G. Munuswamy-Ramanujam, V. Soman, Shree Devi Munusamy Sampangiramulu, S. Parameswaran","doi":"10.5530/ijper.57.3s.72","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.72","url":null,"abstract":"Introduction: Padikara Parpam (PP), is one among the highly regarded Siddha medicine that is been traditionally claimed for its anti-proliferating and anti-carcinogenic properties. Objectives: In the present work, we investigated the cytotoxic effects of PP in a cellular model of Human leukemia (Human monocytic cell lines (THP-1). Materials and Methods: Determination of cell viability was assessed by the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) assay and by using Confocal Laser Scanning Microscope (CLSM). Fluorescence-activated single cell sorting (FACS), and 2'-7'-Dichloro-Dihydro-Fluorescein Diacetate (DCFH-DA) mediated intracellular Reactive Oxygen Species (ROS) measurements were also carried out to understand the effect of PP on THP-1 cells. Results: PP induced cytotoxic effects against THP-1 in a concentration-dependent manner (6.02%–92.7%), with the highest cytotoxicity at 0.5 mg/mL concentration of PP. The IC 50 values of PP in THP-1 cell lines were 0.115 mg/mL. The result from the MTT assay was further confirmed by CLSM reports. The images of Acridine orange/Ethidium bromide stained THP-1 cells treated with PP (IC 50 concentration) indicated red fluorescence compared to the control cells which showed only green fluorescence. The images indicated the induction of apoptosis by the study drug. FACS and DCFH-DA based intracellular ROS measurements indicated the ability of PP to increase intracellular ROS levels in a concentration dependent manner. Thereby indicating at a possible ROS mediated apoptotic mechanism of action. Conclusion: These results suggest that with further clinical studies, PP could be used as an economic and effective anti-leukemic drug in patients suffering from leukemia.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47023154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Analysis of the Extent of Intra and Inter-rater Variability in OSCE 欧安组织国家内部和国家间变异程度的分析

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.58

Swanand S Pathak, Gaurav Vedprakash Mishra, Rupesh A. Warbhe

{"title":"An Analysis of the Extent of Intra and Inter-rater Variability in OSCE","authors":"Swanand S Pathak, Gaurav Vedprakash Mishra, Rupesh A. Warbhe","doi":"10.5530/ijper.57.3s.58","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.58","url":null,"abstract":"Background: OSCE is an objective structured clinical examination, though the tool is designed to increase the objectivity in the examination of the clinical cases, the inter and intra rater variability of the tool needs to analysed. Aim: To determine the extent of intra and inter-rater variability in OSCE. Settings and Design: The study was conducted in a medical college and is a cross sectional study. Materials and Methods: 5 OSCE stations were designed and videos recorded of the candidates performing the clinical exercise, 9 teachers were selected, three of assistant professor grade (junior level), three of associate grade (middle level) and three of professor grade (senior level), all the teachers were shown the videos on day 1 and the scores recorded, the same videos were shown to the teachers on the second day and the scores recorded, each teacher graded all the 5 OSCE recordings. Statistical Analysis Used: Student paired t test, One-Way ANOVA and multiple comparison to Tukey test and Cronbach alpha. Software used for analyses was SPSS 27.0 version. Results: when the scores were segregated according to level of Assessors it was observed that there was significant intra-rater variability at senior level, while there is significant inter-rater variability between junior and mid-level assessors. Conclusion: There is intra and inter-rater variability observed in OSCE assessment when assessors were segregated in groups as per their seniority levels","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49164360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability-Indicating Reversed Phase-HPLC Method Development and Validation for Estimation of Phenylephrine in Bulk and Tablet 稳定性指示反相高效液相色谱法测定原料药和片剂中苯肾上腺素的方法开发与验证

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.90

S. Srivastava, S. Dhaneshwar, N. Kawathekar

{"title":"Stability-Indicating Reversed Phase-HPLC Method Development and Validation for Estimation of Phenylephrine in Bulk and Tablet","authors":"S. Srivastava, S. Dhaneshwar, N. Kawathekar","doi":"10.5530/ijper.57.3s.90","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.90","url":null,"abstract":"Background: This study is aimed to develop a validated stability-indicating method of a nasal decongestant phenylephrine hydrochloride in bulk and tablet. Materials and Methods: A sensitive, accurate, and specific reversed-phase stability indicating HPLC method was developed and validated by following ICH guidelines, for the estimation of Phenylephrine Hydrochloride (PHE) in bulk and tablet. On Luna® 5µm C 18 column (250 × 4.6mm), the isocratic separation was achieved using mobile phase of 5mM ammonium acetate (pH 4.7): methanol (80:20; v/v) with a flow rate of 1 mL/min and a column temperature at 30°C. The proposed method was able to produce good separation of the drug and its degradation products with sharp peaks. The quantification was done at 272 nm by photodiode array detection. Conclusion: It was discovered that the phenylephrine hydrochloride was resilient to photolytic and thermal degradation, but degraded under acid, base, and oxidative stress conditions. The developed method was found to be linear, robust, and accurate and can be successfully applied for identification, quantitative determination, and monitoring of the stability of phenylephrine in bulk and tablet dosage forms.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47416812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Formation Analysis of Ca (II), Mg (II), Zn (II) and 5-Hydroxysalicylic Acid Binary Complexes in Cetyltrimethylammonium Bromide Cationic Micelles 十六烷基三甲基溴化铵阳离子胶束中Ca (II)、Mg (II)、Zn (II)和5-羟基水杨酸二元配合物的形成分析

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.83

M. Ramanaiah, M. Balakrishna, R. Neeraja, Sattaru Gouthamsri, P. Seetharam

引用次数: 1

Synthesis of Cyclopyrrolidine Clubbed with Oxadiazole Bases and Evaluation of their Anti-Diabetic Activity through in vivo Model 恶二唑基环吡咯烷棒状化合物的合成及其体内抗糖尿病活性评价

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.85

Salve Megha Tukaram, Jadhav Shailaja

{"title":"Synthesis of Cyclopyrrolidine Clubbed with Oxadiazole Bases and Evaluation of their Anti-Diabetic Activity through in vivo Model","authors":"Salve Megha Tukaram, Jadhav Shailaja","doi":"10.5530/ijper.57.3s.85","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.85","url":null,"abstract":"Background and Aim: Based on inhibitors of DPP-IV, there is a fantastic method for creating anti-diabetic medications. These inhibitors regulate diabetic patients' blood sugar levels to prevent difficulties with their health. In the current work, we created a brand-new series of compounds Cyclopyrrolidine clubbed with oxadiazole bases. Materials and Methods: Cyclopyrrolidine clubbed with oxadiazole bases (B-1 to B-16) were synthesized and characterized through IR, NMR, mass spectrometry, and elemental analysis. Docking studies were performed to assess interactions and binding modes of synthesized hits at the binding site of receptor DPP-4 (PDB 3W2T). Using vildagliptin as a standard drug, six of the synthesized compounds were tested for their antidiabetic activity in diabetic rats induced with HFD-STZ-Nicotinamide. Results: The results showed that compound B-XIV (220*4.56B) resulted in the greatest reduction in blood glucose level from all synthesized compounds compared to that of vildagliptin (215*7.52B) in HFD-STZ-Nicotinamide. Other compounds showed moderate to good antihyperglycemic activity. Conclusion: From he presents work it can be concluded that synthesized compounds possess good DPP-IV inhibitory activity. Compounds containing electron-withdrawing groups (chlorine, nitro, methoxy) were displayed a good anti-diabetic effect than electron-donating groups (methyl, hydroxyl). Oxadiazole derivatives could be used for further development to obtain more promising drug candidates.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42752665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of Analytical Quality by Design Methodology to Develop a UV Spectrometric Technique for Arteether Quantification 用设计方法实现分析质量——建立一种紫外光光谱法测定乙醚含量的方法

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.91

Neha Bajwa, Pallavi Saroch, Ashish Baldi

{"title":"Implementation of Analytical Quality by Design Methodology to Develop a UV Spectrometric Technique for Arteether Quantification","authors":"Neha Bajwa, Pallavi Saroch, Ashish Baldi","doi":"10.5530/ijper.57.3s.91","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.91","url":null,"abstract":"Aim/Background: Analytical Quality by Design refers to applying the Quality by Design idea to the development of analytical procedures. The present study emphasizes the AQbD concept of developing and validating a spectrophotometric technique for detecting α, β-arteether per the ICH Q8 ( R 2 ) requirements for the first time. Materials and Methods: Sample preparation, sample pH, and wavelength were all integrated into the Ishikawa diagram, and essential parameters were obtained. The ratio of ethanolic PBS 6.8 and HCl was taken as factors. At the same time, the drug molecule's absorbance was identified as a critical factor that was further analyzed using a simple mixture design of experiments methodology for method robustness and optimization. The novel, durable, precise, and accurate UV-spectrophotometric method, α, β-arteether, was developed using the Quality by Design principle. By adjusting the HCl concentration (for acid degradation) and ethanolic PBS 6.8, the highest absorption of α, β-arteether was achieved by heating at 50°C for 30 min. Results and Discussion: The percent RSD less than 2, R 2 > 0.99 was recorded for the concentration range of 2–20 μg/mL at λ max 254 nm. The developed method's LOD and LOQ were within acceptable limits. The presented approach might be used at the industrial level as a rapid, precise, accurate, and cost-effective quality control method for a frequent and simultaneous estimate of α, β-arteether.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41908235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Evaluation of Nanoemulsion of the Phenolic Content of Foeniculum vulgare for Antidepressant and Antihypertensive Potentiality 小茴香酚含量纳米乳抗抑郁降压作用的制备及评价

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.75

Sanjita Das, S. Rani

{"title":"Formulation and Evaluation of Nanoemulsion of the Phenolic Content of Foeniculum vulgare for Antidepressant and Antihypertensive Potentiality","authors":"Sanjita Das, S. Rani","doi":"10.5530/ijper.57.3s.75","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.75","url":null,"abstract":"Objectives: Foeniculum vulgare (common name: fennel; family: Umbelifereae) is a well-documented plant with various medicinal properties to manage different types of disease. Depression and hypertension are associated with each other. The study focuses mainly on the evaluation of the antidepressant and antihypertensive effect of the nanoemulsion formulations of the phenolic content of the arial part of Foeniculum vulgare . Materials and Methods: The nanoemulsion formulation (1% and 2% W/V) formulation was formulated and subjected to in vivo screening of its antidepressant activity following forced swim and tail suspension tests in mice, while its antihypertensive effect using salt induces hypertension in wistar rats with BIOPAC power lab. Results: The study measured a significant ( p <0.01) reduction ( p <0.01) by Nanoemulsion of Foeniculum vulgare (NFV) in immobility times in mice, which was well comparable to imipramine (15 mg/kg) and so significant ( p <0.01) normalization ( p <0.01) of increased blood pressure. It was further interpreted significant ( p <0.01) increase in the brain level of dopamine and serotonin exhibiting major mechanism behind the antidepressant and antihypertensive potentiality of Foeniculum vulgare . Conclusion: The study suggested the implementation of the use of NFV in the overall treatment of hypertension associated with depression and vice versa.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46734625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiparticulate Floating Beads an Aid to Enhance Therapeutic Efficacy of Rabeprazole 多关节漂浮珠辅助提高雷贝拉唑疗效

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.62

Prajakta Magdhut, P. Dandagi, Umashri A Kokatanur, A. Patil

{"title":"Multiparticulate Floating Beads an Aid to Enhance Therapeutic Efficacy of Rabeprazole","authors":"Prajakta Magdhut, P. Dandagi, Umashri A Kokatanur, A. Patil","doi":"10.5530/ijper.57.3s.62","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.62","url":null,"abstract":"Background: Rabeprazole sodium is a newer generation anti-ulcer drug with short half-life and low bioavailability. Present research work is an attempt to design novel floating beads of Rabeprazole sodium in multiparticulate dosage form to increase residence time and modulate its release behavior for stomach-specific delivery. Materials and Methods: In this present study, Rabeprazole sodium beads were formulated by ionotropic gelation method and effect of variation in sodium alginate and gellan gum concentrations alone and in combination on release properties was examined. Results: Formulated beads were analyzed for particle size, density, entrapment efficiency, swelling index, in vitro buoyancy properties, surface topography, in vitro drug release and release kinetics study. The percentage content and entrapment efficiency of Rabeprazole sodium in beads ranged from 77.06±3.612 to 92.88±5.723 and 57±1.543 to 89±1.089 respectively. In vitro drug release of Rabeprazole sodium from the beads at the end of 12 hr ranged from 69.373% to 97.0142%. The release behavior was best fitted in Korsemeyer-Peppas equation. F9 was optimized depending on entrapment efficiency, in vitro buoyancy properties and in vitro drug release. Modified formulation F9 was also subjected to a series of tests. mucoadhesive study , in vivo X-ray imaging in rabbits and stability study. Conclusion: These studies revealed that beads exihibited 84% mucoadhesion, floating up to 12 hr as well as stability at 25±2°C. Hence Floating bead formulation of Rabeprazole sodium will be a promising drug delivery system to improve drug residence time and patient compliance.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45358021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoemulgel Formulation of Tetrahydrocurcumin with Efficient Anti-inflammatory Effect for the Treatment of Skin Disorders 具有高效抗炎作用的四氢香豆素纳米乳液制剂治疗皮肤疾病

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.61

K. Sharma

{"title":"Nanoemulgel Formulation of Tetrahydrocurcumin with Efficient Anti-inflammatory Effect for the Treatment of Skin Disorders","authors":"K. Sharma","doi":"10.5530/ijper.57.3s.61","DOIUrl":"https://doi.org/10.5530/ijper.57.3s.61","url":null,"abstract":"Introduction: Tetrahydrocurcumin (THC) is a partially reduced white metabolite of curcumin which does not impart yellowish colour on skin due to which it seemed to be a promising topical agent compared to curcumin which discoloured the skin with long lasting visible mark. Objectives: The research work was intended to formulate THC loaded Nano Lipid-based (TNL) Carbopol Gum Gel (TNLCG) also called tetrahydro curcumin nanoemulgel and to investigate its therapeutic efficacy against inflammation using animal model. Materials and Methods: TNLCG was prepared based on pseudoternary phase-diagram with solubilizing capacity of tocopheryl acetate (10% w/w) and emulsifying combination of tween 80: polyethylene-glycol 400 (2:1 in 35% w/w) in 45% w/w aqueous phase with Carbopol 934 gel base. Selected formulations were evaluated for various parameters using in-house developed HPLC analytical method (at 280nm). Results: Nanoemulgel was found to be a stable formulation which significantly improved cellular absorbency with tocopheryl acetate with high concentration of THC for treating skin inflammation. Significant increase in the steady state flux (Jss) of 41µg/cm 2 /h, permeability coefficient (Kp) of 1.08 and Enhancement ratio (Er) of 3.7 were observed. The TNLCG demonstrated 77.36% in vitro drug release, significant skin permeability and optimal properties including spherical shape with 129nm nanosize, adequate zeta potential (-21.45mV), and PDI value of 0.18. Conclusion: This study revealed encouraging outcomes for TNLCG formulation as a novel tool for safe delivery of THC. According to the findings of the preceding research, it can be an effective therapeutic formulation which offers significant inflammation reducing activity with good moisturising quality.","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42061341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Innovative UV-spectroscopic Approaches for Simultaneous Assessment of Celecoxib and Tramadol Hydrochloride in Binary Mixture 同时评价塞来昔布和盐酸曲马多二元混合物的不同创新紫外光谱方法

IF 0.8 4区医学

Indian Journal of Pharmaceutical Education and Research Pub Date : 2023-08-23 DOI: 10.5530/ijper.57.3s.89

A. Sen, Preksha Darji, D. Sen, A. Zanwar, C. Aundhia, R. Maheshwari

引用次数: 0