Indian Journal of Microbiology最新文献

{"title":"A Chronicle Review of In-Silico Approaches for Discovering Novel Antimicrobial Agents to Combat Antimicrobial Resistance","authors":"Nagarjuna Prakash Dalbanjan, S. K. Praveen Kumar","doi":"10.1007/s12088-024-01355-x","DOIUrl":"https://doi.org/10.1007/s12088-024-01355-x","url":null,"abstract":"<p>Antimicrobial resistance (AMR) poses a foremost threat to global health, necessitating innovative strategies for discovering antimicrobial agents. This review explores the role and recent advances of <i>in-silico</i> techniques in identifying novel antimicrobial agents and combating AMR giving few briefings of recent case studies of AMR. <i>In-silico</i> techniques, such as homology modeling, virtual screening, molecular docking, pharmacophore modeling, molecular dynamics simulation, density functional theory, integrated machine learning, and artificial intelligence, are systematically reviewed for their utility in discovering antimicrobial agents. These computational methods enable the rapid screening of large compound libraries, prediction of drug-target interactions, and optimization of drug candidates. The review discusses integrating <i>in-silico</i> approaches with traditional experimental methods and highlights their potential to accelerate the discovery of new antimicrobial agents. Furthermore, it emphasizes the significance of interdisciplinary collaboration and data-sharing initiatives in advancing antimicrobial research. Through a comprehensive discussion of the latest developments in <i>in-silico</i> techniques, this review provides valuable insights into the future of antimicrobial research and the fight against AMR.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"40 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141781236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Extracellular Polymeric Substances from Biofilm-Forming Marine Bacteria from the Arabian Sea, India","authors":"Madhav Kumar, Riya Soni, Rajiv S. Maniyar, Pooja Saran, Doongar R. Chaudhary","doi":"10.1007/s12088-024-01351-1","DOIUrl":"https://doi.org/10.1007/s12088-024-01351-1","url":null,"abstract":"<p>Extracellular polymeric substances (EPS) are complex, hydrated matrices produced by biofilm-forming bacteria to anchor themselves to surfaces and resist antibiotic treatment. EPS plays a critical role in the formation, maintenance and virulence of biofilms, leading to persistent infections and posing significant challenges in healthcare. Characterizing bacterial EPS is essential to understand their biochemical composition and functional properties, which is critical for optimizing their applications in biotechnology, medicine, and environmental management. Therefore, the present study aimed to isolate and screen the bacteria from Arabian Sea for their ability to produce EPS from biofilm surfaces. Additionally, the detailed characterization of the EPS was also carried out. These bacteria were identified using 16S rRNA gene sequence analysis and revealed that all the EPS-producing bacterial isolates belong to different bacterial genera (<i>Oceanimonas</i>, <i>Psychrobacter</i> and <i>Vibrio</i>). The bacteria were cultured on Zobell marine broth media and EPS were isolated using the propanol precipitation method. The EPS weight varied among the bacterial isolates and ranged from 0.81 g L⁻¹ to 2.21 g L⁻¹. The EPS produced by the bacterial strains have shown antimicrobial as well as free radicals (2,2-diphenyl-1-picrylhydrazyl; DPPH) and 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity. The antimicrobial and free radical scavenging properties of EPS have promising biotechnological applications in developing new antibiotics, enhancing food preservation, creating protective coatings, and improving wound healing therapies. The Fourier-transform infrared spectroscopy (FTIR) revealed the presence of aliphatic methyl, halide groups, saccharides and primary amines. Gas chromatography equipped with mass spectroscopy (GC–MS) confirmed the presence of monosaccharides such as glucose, galactose, arabinose and mannose. The EPS were further characterized using X-Ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The study highlights the importance of EPS in biofilm formation, antibiotic resistance and persistent infections, emphasizing the importance of isolating and characterizing of EPS for its potential biotechnological applications, including antimicrobial and free radical scavenging activities.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"33 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141718297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Real-Time PCR for Accurate Identification and Quantification of Common Probiotic Bacteria in Iranian Probiotic Dairy Products","authors":"Setayesh Zamanpour, Vida Eraghi, Mohammad Hashemi, Mahdi Ram, Asma Afshari","doi":"10.1007/s12088-024-01325-3","DOIUrl":"https://doi.org/10.1007/s12088-024-01325-3","url":null,"abstract":"<p>The effectiveness of probiotics for health depends on the strain type and dosage. In this study, we endeavored to establish a robust quantitative Real-time PCR method for the evaluation probiotic strains in Iranian probiotic dairy products. The process was initiated by extracting DNA from selected bacterial strains and selecting well-matched primers. Then, we optimized the Real-time PCR method to efficiently identify and quantify three prevalent beneficial bacterial strains, including <i>Lactobacillus acidophilus</i>, <i>Lactobacillus casei</i>, and <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> in four types of Iranian probiotic yogurt with different brands simultaneously and within 4 h. To overcome complications such as sample variability or interference with other common microorganisms in dairy products, we simultaneously used IPC and specific primers of each strain. The sensitivity and specificity obtained in the present study were 10⁶ CFU/mL for the targeted bacterial strains. The relative standard deviation (%RSD) was maintained at less than 2% regarding repeatability and reproducibility. The method designed and validated for identifying and counting these strains exhibits remarkable specificity, sensitivity, accuracy, optimal reaction efficiency, and minimal variance. Collectively, our method has proven to be specialized, cost-effective, and rapid, which makes it suitable for qualitative evaluation of probiotic dairy products in the industry.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"7 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141610707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Frontiers: Graphene-Based Nano-biosensor Platforms for Cutting-Edge Research and Future Innovations","authors":"Niket Rana, Jasjeet Narang, Arjun Chauhan","doi":"10.1007/s12088-024-01318-2","DOIUrl":"https://doi.org/10.1007/s12088-024-01318-2","url":null,"abstract":"<p>Graphene and its derivatives have excellent electrical, mechanical, and optical capabilities, making it the perfect foundation for sensing living things. Graphene-based nano biosensors have shown exceptional sensitivity, selectivity, and quick response times when used to detect a range of analytes, such as biomolecules, cells, and pathogens. The main uses of graphene-based nano biosensors are disease diagnosis, environmental monitoring, food safety, and drug development. It also explores prospective future strategies, such as methods for functionalizing nanomaterials, their incorporation with other nanomaterials, and the creation of wearable and implantable gadgets. Various signalling techniques, such as fluorescence, electrochemistry, surface plasmon resonance, surface-enhanced Raman scattering, etc., can be coupled with graphene-based biosensors to quantitatively detect disease-associated DNA, RNA, and protein biomarkers quantitatively. Graphene-based nano biosensors, combined with cutting-edge innovations like artificial intelligence and the Internet of Things, can completely transform industries like healthcare and environmental monitoring. Developing these biosensors with high sensitivity and low detection limits provides a new direction in medical and personal care. The later portion of the review covers the difficulties, prospective fixes, and opportunities of graphene-based biosensors.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"88 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, Purification, and Characterization of a Novel Exopolysaccharide from Probiotic Lactobacillus amylovorus: MTCC 8129","authors":"Janani Murugu, Rajnish Narayanan","doi":"10.1007/s12088-024-01346-y","DOIUrl":"https://doi.org/10.1007/s12088-024-01346-y","url":null,"abstract":"<p>Extracellular polysaccharides (EPS) produced by Lactic Acid Bacteria have an individual effect on the flavour and consistency of novel food materials, as well as potential therapeutic applications. The purpose of this study was to create, improve, and characterise EPS from <i>Lactobacillus amylovorus</i> MTCC 8129. FTIR examination showed the compound's composition (acetyl group, hydroxy group, ring structure) as well as the numerous interlinks between sugar residues, which were then validated by Nuclear Magnetic Resonance Spectroscopy. Thermogravimetric examination showed that the EPS exhibited resistance to heat at a temperature of 640 °C, with antioxidant levels ranging from 70 to 85% and emulsification activity above 50%. Furthermore, it has 180% water holding capacity and 140% oil holding capacity. Based on these findings, it seems that the EPS that was reviewed might potentially be an advantageous addition to the food processing industry.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"149 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Methylocucumis oryzae, a Large-sized, Phylogenetically Unique Type Ia Methanotroph with Biotechnological Potential","authors":"Monali C. Rahalkar, Jyoti A. Mohite, Kajal Pardhi, Shubha S. Manvi, Yash S. Kadam, Yukta V. Patil","doi":"10.1007/s12088-024-01347-x","DOIUrl":"https://doi.org/10.1007/s12088-024-01347-x","url":null,"abstract":"<p>The cultivation of microorganisms is important as it provides us an opportunity to explore the characteristics that can be used for a variety of applications. Methanotrophs oxidize methane and are one of the most challenging organisms to culture. In 2018, we cultured one of the largest methanotrophs within the gammaproteobacterial group (Type Ia), a novel genus and species, <i>Methylocucumis oryzae</i>, with large peculiar, elongated oval (cucumber-shaped) cells (~ 3–6 µm length × 1.5 µm breadth). We have described three strains of <i>Methylocucumis oryzae</i> (abbreviated as Mcu or <i>Mcu oryzae</i>) isolated from two flooded rice fields and recently from a stone quarry in Pune, all three locations are in Maharashtra state, Western India. Mcu is a mesophile and prefers lower temperatures for growth in the range 20–28 °C and does not grow above 37 °C. No other species of <i>Methylocucumis</i> have been reported from any other country and Mcu oryzae appears to be phylogenetically unique after 6 years of its initial report. Though the culture has certain challenges to grow on a larger scale due to its slow growth rate, it might have significant potential in methane mitigation, plant growth promotion, carotenoid production, etc. awaiting more detailed studies on this rare organism. Further optimization experiments to grow Mcu in large quantities might help us in developing environmental and biotechnological applications.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"25 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Vaginal Microbiome Associated with Oncogenic HPV Infection Among Different Ethnic Groups of Women of the Eastern Region of India","authors":"Anindita Goswami, Suvanjana Ghosh, Arghya Bandyopadhyay, Raju Gopal Saha, Prabir Sengupta, Utpal Bhuniya, Paramita Mandal","doi":"10.1007/s12088-024-01320-8","DOIUrl":"https://doi.org/10.1007/s12088-024-01320-8","url":null,"abstract":"<p>The study aimed to identify the influence of vaginal bacterial composition on HPV infection among tribal women of the eastern region of India compared to non-tribal women of the same region. For this study, 13 tribal women and 12 non-tribal women were recruited. DNA was isolated from vaginal swab samples, and subsequently, 16S rRNA gene analysis was performed. We identified two distinct clusters of samples based on taxonomic profiling and bacterial diversity. One cluster belonged to HPV negative samples and the other to HPV16/18 positive samples. The abundance of three bacterial species was significantly lower (<i>p</i> value &lt; 0.05) among oncogenic HPV positive samples (mean abundance = 4.33, 0, and 0, respectively) compared to HPV negative samples (mean abundance = 29.71, 45.73, and 19.01, respectively) irrespective of their ethnicities, such as <i>Lactobacillus amylolyticus, Bacillus coagulans, and Costridium </i>sensu stricto<i>.</i> HPV16/18 positive samples also represent the differential microbiome composition between the two ethnic groups of women. Ethnicity specific variations in human vaginal microbiome composition might be recommended for geographically tailored microbiome-based therapeutic strategies.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"71 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence and mRNA Expression of the sar Family Genes in Clinical and Non-clinical (Healthy Conjunctiva and Healthy Skin) Isolates of Staphylococcus epidermidis","authors":"Mario E. Cancino-Diaz, Fernando Gómez-Chávez, Juan C. Cancino-Diaz","doi":"10.1007/s12088-024-01339-x","DOIUrl":"https://doi.org/10.1007/s12088-024-01339-x","url":null,"abstract":"<p><i>Staphylococcus aureus</i> possesses <i>sar</i> family genes, including <i>sar</i>A, S, R, T, U, V, X, Y, Z, and <i>rot</i>, which are transcription factors involved in biofilm formation and quorum sensing. In contrast, <i>Staphylococcus epidermidis</i> has <i>sar</i>A, R, V, X, Y, Z, and <i>rot</i> genes; specifically, SarA, Z, and X are involved in biofilm formation. The expression of the <i>sar</i> family members in <i>S. epidermidis</i> isolated from clinical and non-clinical environments is unknown. This study aimed to establish if clinical and non-clinical isolates of <i>S. epidermidis</i> express the <i>sar</i> family members. We genotyped isolates from clinical ocular infections (n = 52), or non-clinical healthy conjunctiva (n = 40), and healthy skin (n = 50), using multilocus sequence typing (MLST) and the staphylococcal chromosomal cassette <i>mec</i> (SCC<i>mec</i>). We selected strains with different genotypes and representatives of each source of isolation, and the presence of the <i>sar</i> family genes was detected using PCR and RT-qPCR to determine their expression. The <i>sar</i> family genes were present in all selected strains, with no observed differences. The relative expression of the <i>sar</i> family showed that all selected strains expressed each gene weakly, with no significant differences observed between them or between different sources of isolation. In conclusion, the presence and relative expression of the <i>sar</i> family genes are very similar among strains, with no differences based on their origin of isolation and genotype.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"12 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Analysis of Classical Swine Fever Virus Associated Field Infections Evidence Novel CSFV Sub Genotype in Tamil Nadu, Southern India","authors":"S. Parthiban, B. Kowsalya, M. Parthiban, A. Ramesh, P. Raja, K. Gopal, S. Jaisree, R. Thangathurai, K. Senthilkumar","doi":"10.1007/s12088-024-01345-z","DOIUrl":"https://doi.org/10.1007/s12088-024-01345-z","url":null,"abstract":"<p>Classical swine fever (CSF) is an endemic and major viral infection of Indian swine husbandry, contributing to great economic losses with multiple genotypes associated with vast clinical and subclinical outcomes. Molecular detection and genotyping of CSF virus directly from field samples has great application in disease monitoring and control measures hence this study aimed to isolate and characterize CSFV genotypes circulating in southern states of India. Fifty-seven porcine post-mortem tissues (lymph nodes, spleens, livers, lungs, and kidneys) collected from pigs suspected of systemic infections and sudden death with the history of live attenuated CSF vaccination from different regions of Tamil Nadu were used in this study. An <i>NS5B</i> gene based CSFV specific RT-PCR screening confirmed CSFV positivity in 7% (4/57) of samples with a specific amplicon of 449 bp. Further molecular screening for other viral co-infections such as PCV2, PPV and PRRSV done by specific individual PCR assays to all the samples. Non-involvement of above screened three viral pathogens in all four field samples which showed positivity for CSFV confirming CSFV as primary pathogen<b>.</b> Two RT-PCR positive samples (TNI-4 and CHNL-2) selected randomly and sequenced. Aligned contig sequences of both samples were subjected to BLAST homology search and phylogentic characterization. BLAST study of TNI-4 sequence revealed 99% sequence identity with Indian CSFV sequences of genotype 1 and CHNL-2 showed 98% sequence identity with Indian CSFV sequences of genotype 2. Phylogenetic analysis of the TNI-4 and CHNL-2 sequences obtained in this study along with 38 published CSFV sequences consisting of all 5 new genotypes and 14 sub genotypes through the Maximum Likelihood tree method in MEGA 11 revealed that TNI-4 clustering together with 1.7 sub genotypes and CHNL-2 clustering together with 2.2 sub genotypes. TNI-4 and CHNL-2 partial <i>NS5B</i> gene sequences obtained in this study deposited in the GenBank database under accession numbers of MW822568 and MW822569 respectively. The study is the first to report CSF infections associated with the newer 1.7 sub genotype in Tamil Nadu, southern India. It is possible that vaccination could affect the genetic diversity of the CSFV through recombination and point mutations for immune evasion.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"61 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141510959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Gut Microbiome in Cancer Treatment","authors":"Vijay Mishra, Yachana Mishra","doi":"10.1007/s12088-024-01340-4","DOIUrl":"https://doi.org/10.1007/s12088-024-01340-4","url":null,"abstract":"<p>The gut microbiota influences the effectiveness and side effects of cancer treatments, particularly immunotherapy and associated immune-related complications. This important involvement of the microbiome is supported by the patients receiving antibiotics responding poorly to immunotherapy. Relatively few research has examined the underlying processes, and until recently, data regarding the detection of the microbial organisms that trigger these effects were inconsistent. Since then, a deeper comprehension of the processes of action and taxonomic classification of the relevant species has been attained. It's been demonstrated that certain bacterial species can enhance the body’s reaction to immune checkpoint inhibitors through the release of distinct metabolites or products. Nonetheless, in certain patients who are not responding, Gram-negative bacteria may have a dominating suppressive impact. Patients' propensity to react to immunotherapy can be somewhat accurately predicted by machine learning techniques based on their microbiome makeup. Consequently, there has been an increase in interest in modifying the microbiome makeup to enhance patient reaction to medication. Clinical proof-of-concept studies demonstrate that dietary modifications or fecal microbiota transplantation (FMT) might be used therapeutically to increase the efficacy of immunotherapy in cancer patients. Current developments and new approaches for microbiota-based cancer treatments have been emphasized. In conclusion, preclinical research on animals and human clinical trials has made tremendous progress in our understanding of the function of the gut microbiome in health and illness. These investigations have shed light on the effects of food, FMT, probiotics, prebiotics, and microbiome-disease connections. However, there are still a lot of issues and restrictions that must be resolved before this research can be used in real-world clinical settings.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"205 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141530237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}