Indian Journal of Pharmaceutical Sciences最新文献

{"title":"Inhibitory Effect of Ouabain on Resistance to Imatinib in Chronic Myeloid Leukemia K562/G01 Cells","authors":"Chang-Quan Sun, J. Liu, Shiqi Zhai, H. Liu, L. Peng, Jing Hu","doi":"10.36468/pharmaceutical-sciences.1129","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1129","url":null,"abstract":"Ouabain has shown powerful anti-proliferation activities in various cancers, but its effect on imatinib-resistant chronic myeloid leukemia and toxicity on normal mice has not been investigated. Cell Counting Kit-8 assay was used to detect cytotoxicity and reversal effect of ouabain with different concentration (0.01 µM, 0.1 µM, 1.0 µM, 10 µM) on drug resistance of imatinib-resistant cell line of chronic myeloid leukemia (K562/G01 cell line). Flow cytometry was used to detect the apoptosis effect and cell cycle arrest. Hematological examination, biochemical examination and histological examination were used to detect sub-chronic toxicity of ouabain on healthy mice. In our present study, ouabain showed greatly inhibitory effect and significantly reduced half minimal inhibitory concentration of imatinib in K562/ G01 cells, an imatinib-resistant cell line of chronic myeloid leukemia, in a dose-and time-dependent manner, which implied that ouabain increased cell sensitivity to imatinib. Ouabain enhanced apoptosis induced by imatinib in K562/G01 cells not through cell cycle arrest. Animal experiments showed that there were no significant variances in hematological, liver function, kidney function parameters and organ histopathology of all mice groups. These data suggested that ouabain could be a potential agent to treat imatinib-resistant chronic myeloid leukemia for its powerful cytotoxicity as well as reversal effect, but further study is needed to find out its specific mechanism.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69606862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Calponin 2 as a Tumor Inhibitor in Esophageal Cancer","authors":"M. Gan, Yuhui Xia, Yixiao Pan, Qingxin Yu, Yiqing Guo, Yuyi Feng, J. Zheng","doi":"10.36468/pharmaceutical-sciences.1137","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1137","url":null,"abstract":"Calponin is an actin filament-associated regulatory protein that can inhibit the activity of myosin-ATPase and stabilize the dynamics of the actin cytoskeleton. Although calponin 2 has been reported to play roles in several cancers, whether it takes part in the progression of esophageal cancer still remains unknown. To explore the pathologic significance of calponin 2 in esophageal squamous cell carcinoma, the expression level of calponin 2 proteins in the tumor tissue of 190 esophageal squamous cell carcinoma patients was examined with immunohistochemistry while the expression level of calponin 2 messenger ribonucleic acid was analyzed by using the data from The Cancer Genome Atlas database. Both the calponin 2 messenger ribonucleic acid and protein level were increasingly expressed in the tumor tissues of esophageal squamous cell carcinoma patients compared with the adjacent non-tumor tissue and correlated negatively with the tumor grade. Patients with higher calponin 2 in the tumor tissue were found to have longer overall survival time. Calponin 2 was shown to be an independent factor influencing the overall survival of the esophageal squamous cell carcinoma patients. Methylation analysis based on MethSurv database revealed a methylation site in the body of calponin 2 gene, which was associated with a better prognosis. Further, in esophageal cancer tumor tissue, calponin 2 gene was found to co-express with genes associated with tight junction and the expression level of calponin 2 was observed to correlate significantly with the number of infiltrating immune cells. These results supported the idea that calponin 2 is involved in esophageal cancer and may function as a tumor inhibitor probably through modulating cancer cells tight junction and tumor immunity.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69607355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total Flavones of Selaginella uncinata (Desv.) Spring Inhibits Breast Cancer Cell Proliferation and Induces Apoptosis via Regulating microRNA-1269","authors":"Lideng Ni, Xu-lin Wang, J. Gu, Shuai Hao, Liyang Sun","doi":"10.36468/pharmaceutical-sciences.1149","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1149","url":null,"abstract":"","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69607606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and Evaluation of Self-Emulsifying Drug Delivery Systems of Larotaxel for Oral Administration","authors":"J. Shan, Yuan Tian, G. Jingxin, H. Haibing, W. Yanjiao, YU Z, T. Xing","doi":"10.36468/pharmaceutical-sciences.1150","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1150","url":null,"abstract":"The objective of the present study was to develop a self-emulsifying drug delivery system of larotaxel and evaluate its in vitro and in vivo performance. The prepared larotaxel loaded self-emulsifying drug delivery system spontaneously formed microemulsion with a mean particle size of 115.4 nm when mixed with 100-fold water under gentle agitation. In vivo pharmacokinetics study of larotaxel loaded self-emulsifying drug delivery system demonstrated 5.19-fold enhancement in oral bioavailability compared to larotaxel-Sol. Furthermore, intestinal bio-distribution studies revealed that the intestinal residence time of larotaxel loaded self-emulsifying drug delivery system was dramatically extended in comparison to larotaxel-Sol. Lymphatic transport studies showed that cycloheximide, a chylomicron secretion blocker, would impede oral absorption of larotaxel loaded self-emulsifying drug delivery system, which confirmed that lymphatic route was involved in the absorption of larotaxel loaded self-emulsifying drug delivery system. What is more, in vivo antitumor experiment proved that the antitumor activity of oral larotaxel loaded self-emulsifying drug delivery system was significantly superior to oral larotaxel-Sol, which was close to intravenous larotaxel-Sol. In conclusion, self-emulsifying drug delivery system is a promising vehicle for the oral delivery of larotaxel.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69607662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TGF-β/Smad/ADAMTS-7 Axis Regulates the Process of Curcumin in Promoting the Cartilage Cells Proliferation","authors":"X. B. Niu, Jiangying Zhang, Y. C. Wang, Lei Guo, Y. X. Liu, W. Liu, XU Y.S.","doi":"10.36468/pharmaceutical-sciences.1151","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1151","url":null,"abstract":"To explore the function of curcumin on the cartilage cells, the cell proliferation and apoptosis by transforming growth factor-beta/suppressor of mothers against decapentaplegic/a disintegrin and metalloproteinase with thrombospondin motifs-7 axis was the objective of the study. The cell viability was detected by cell counting kit-8. The expression of transforming growth factor-beta, suppressor of mothers against decapentaplegic protein, a disintegrin and metalloproteinase with thrombospondin motifs-7, caspase-9, B-cell lymphoma 2, Bcl-2-associated X protein was determined by Western blot. The cartilage cells were treated with 1 mmol/l sodium nitroprusside for 24 h. Then, the cells were treated with different concentration of curcumin for 24 h. We found that 1 μmol/l curcumin could recover the cartilage cells proliferation. The transforming growth factor-beta and suppressor of mothers against decapentaplegic protein show high expression and a disintegrin and metalloproteinase with thrombospondin motifs-7 was low in the curcumin treatment group. Meanwhile, the apoptosis pathway was also detected. The caspase-9 and B-cell lymphoma 2 was higher in the curcumin treatment group than without curcumin group. However, the Bcl-2-associated X protein was lower. The cell viability, a disintegrin and metalloproteinase with thrombospondin motifs-7, caspase-9, B-cell lymphoma 2 and Bcl-2-associated X proteins also show no changes with or without curcumin when the transforming growth factor-beta was inhibited. We found that a disintegrin and metalloproteinase with thrombospondin motifs-7 show over expression, the transforming growth factor-beta and suppressor of mothers against decapentaplegic protein show high expression in the curcumin treatment group, the cell viability, caspase-9, B-cell lymphoma 2 and Bcl-2-associated X proteins show no changes with or without curcumin. Curcumin can promote the cartilage cells proliferation via transforming growth factor-beta/suppressor of mothers against decapentaplegic/a disintegrin and metalloproteinase with thrombospondin motifs-7 axis and inhibit the cell apoptosis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69607713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Adverse Reactions and Effects of Triamcinolone Acetonide Combined with Compound Betamethasone in Keloid","authors":"W. Zhang, Jincui Shen, B. Zeng","doi":"10.36468/pharmaceutical-sciences.spl.689","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.spl.689","url":null,"abstract":"Zhang et al. : Effects of Triamcinolone Acetonide Combined with Compound Betamethasone in Keloid To explore the drug effect and adverse reaction of triamcinolone acetonide combined with compound betamethasone in keloid is the objective of the study. 100 patients were randomly divided into study group and control group (50 patients in each group). The research group received sequential treatment with triamcinolone acetonide injection combined with compound betamethasone injection and the control group received compound betamethasone injection. Both groups had 3 courses of treatment and each course of treatment lasted for 3 w. The Vancouver scar scale score, treatment effect and adverse reactions were compared between the two groups and the recurrence rate was followed up for 1 y after the end of medication. Before treatment, there was no significant difference in Vancouver scar scale scores between the two groups but after medication, the study group was better than that of the control group (p<0.05). The total effective rate of the study group (82 %) was higher than that of the control group (54 %, p<0.05). The total incidence of adverse drug reactions in the study group was 8 % while in the control group it was 6 % and the comparison between the two groups was not statistically significant. The recurrence rates of the study group and the control group were 28 % and 34 % 6 mo after the end of medication and 32 % and 42 % after 12 mo. There was a significant difference between the two groups. The sequential injection of triamcinolone acetonide combined with compound betamethasone is effective in the treatment of keloids, with high safety and low recurrence rate, which is worthy of clinical recommendation.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Lung Microbiota Dysbiosis and Sepsis Induced Acute Respiratory Distress Syndrome","authors":"Jingkun Ye, Zhuandi Lin, Yuxia He, Shan Li","doi":"10.36468/pharmaceutical-sciences.spl.692","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.spl.692","url":null,"abstract":"Ye et al. : Microbiota Dysbiosis in Sepsis Induced Acute Respiratory Distress Syndrome Sepsis is associated with acute respiratory distress syndrome, which has a significant impact on the prognosis of patients. Several studies have shown that the microbiota plays a significant role in sepsis-induced acute respiratory distress syndrome, but the relationship between the microbiota and sepsis-induced acute respiratory distress syndrome is not fully understood. We conducted a case-control and single-center study in 19 sepsis-induced acute respiratory distress syndrome patients and 36 sepsis-non-induced acute respiratory distress syndrome patients to investigate the clinical features and microbiota expression. There were 55 subjects enrolled, 19 of whom suffered acute respiratory distress syndrome due to sepsis. A significant increase in the abundance of Pseudomonas aeruginosa , leptospiral virus, Cytomegalovirus , Klebsiella pneumoniae , Streptococcus pneumoniae , Candida albicans , Escherichia coli , Epstein-Barr virus and Staphylococcus aureus . Besides, expressions of peripheral T lymphocytes (cluster of differentiation 3 + , cluster of differentiation 4 + and cluster of differentiation 3 + , cluster of differentiation 8 + ) was much higher in the sepsis-induced acute respiratory distress syndrome group than that in the sepsis non-induced acute respiratory distress syndrome group. The acute physiology and chronic health evaluation II scores, duration of mechanical ventilation and mortality with 28 d and 90 d were much higher in the sepsis-induced acute respiratory distress syndrome group. Patients with sepsis-induced acute respiratory distress syndrome had worse clinical outcomes and a higher expression of peripheral T lymphocytes, as well as the relative abundance of microbiota dysbiosis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Heart Rate Variability in Obstructive Sleep Apnea Hypoventilation Syndrome","authors":"Yanling Hong, Yan Kou, Mingli Xie, Yong Wan, Yuyan Zhang","doi":"10.36468/pharmaceutical-sciences.spl.682","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.spl.682","url":null,"abstract":"NN50 and high frequency in group C were lower than A and B (p<0.05) and the low frequency was higher than the proportion of NN50 and high frequency in group C were lower than A and B (p<0.05) and the low frequency was higher than group A, group B and control group (p<0.05). Holter-monitoring is a non-invasive test, the price is not high and it can be a regular test for testing the autonomic nervous function in patients with obstructive sleep apnea hypopnea syndrome.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxymatrine Suppresses Colon Cancer by reducing microRNA-21 and microRNA-141 and Targets Epithelial-Mesenchymal Transition","authors":"Yifeng Zhao, Mingxia Li, Chao Zhang, Xiaomin Hu, Xiong Wang, T. Zhao","doi":"10.36468/pharmaceutical-sciences.spl.621","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.spl.621","url":null,"abstract":"","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rd Attenuates Lung Ischemia/Reperfusion Injury in Mice via Regulating Mitochondrial Function","authors":"Si-Fei Yu, Qiong Song","doi":"10.36468/pharmaceutical-sciences.spl.624","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.spl.624","url":null,"abstract":"","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}