Indian Journal of Pharmaceutical Sciences最新文献

{"title":"Loganin inhibits Liver Cancer Cell Proliferation and Metastasis via Blocking Mitogen-Activated Protein Kinase Signaling Pathway","authors":"Jiajun Chen, Yan Qu, Fu Li, Haiyang Zhang, Xiwen Zhang, Xuedong Cao","doi":"10.36468/pharmaceutical-sciences.1262","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1262","url":null,"abstract":"To investigate the action of loganin on the oncogenic phenotypes of liver cancer cells. Huh7 cells were treated with loganin, or loganin and 20 μmol/l mitogen-activated protein kinase signaling pathway inhibitor SP600125. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and Transwell assays detects cell abilities. Western blot detects E-cadherin, N-cadherin, vimentin, phosphorylated-extracellular signal-regulated kinase, extracellular signal-regulated kinase, phosphorylated-p38, and p38 protein expressions. Cell survival rate, the number of migratory and invasive Huh7 cells were significantly reduced with the increasing dose of loganin. E-cadherin content was increased, and contents of N-cadherin, vimentin, phosphorylated-extracellular signal-regulated kinase, and phosphorylated-p38 proteins were decreased in cells with the increasing dose of loganin. SP600125 could enhance the effects mediated by loganin on Huh7 cells. Loganin could significantly restrain the proliferation and metastasis of liver cancer cells by inhibiting the mitogen-activated protein kinase pathway.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/loganin-inhibits-liver-cancer-cell-proliferation-and-metastasis-emviaem-blocking-mitogenactivated-protein-kinase-signali.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Regulating of TGFBR1 Expression by Bone Marrow Mesenchymal Stem Cell-Derived Exosome Let-7a and its Mechanism in Diabetic Nephropathy Rats","authors":"Zirui Pang, Xiaoxi Chen, Linbo Zhang","doi":"10.36468/pharmaceutical-sciences.1269","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1269","url":null,"abstract":"To explore the renal protection mechanism of targeted regulating of transforming growth factor beta receptor type 1 expression by let-7a in diabetic nephropathy rats, diabetic nephropathy rats were created through high-sugar and high-fat diet combined with a small dose of streptozotocin. Normally reared rats were taken as control group. Diabetic nephropathy rats were divided into two groups, one group was treated with let-7a analogs group, and the other group was not treated (diabetic nephropathy group). Experimental results showed that diabetic nephropathy rats had symptoms such as increased dietary frequency, weight loss, proteinuria, and decreased creatinine clearance. After let-7a treatment, the proteinuria symptoms of rats in let-7a analogs group were notably improved, and the creatinine clearance rate increased. The expression level of collagen IV in let-7a analogs group was higher than that of the control, but the difference was not significant. The expression level of fibronectin increased significantly in the diabetic nephropathy group (p<0.05), and that of the let-7a analogs group was significantly lower than that of the diabetic nephropathy group (p<0.05). The immunofluorescence expression result was consistent with the expression trend detected by Western blot. Compared with the control, the expressions of transforming growth factor-beta 1, transforming growth factor beta receptor type 1, and mothers against decapentaplegic homolog 2 in the diabetic nephropathy group were evidently increased (p<0.05), and the expression of mothers against decapentaplegic homolog 7 decreased obviously (p<0.05). The protein expression level was consistent with the messenger ribonucleic acid expression result. In summary, by regulating the levels of collagen IV and fibronectin and targeting the transforming growth factor beta receptor type 1 pathway, let-7a inhibited extracellular matrix synthesis to protect kidney function in patients with diabetic nephropathy.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/targeted-regulating-of-tgfbr1-expression-by-bone-marrow-mesenchymal-stem-cellderived-exosome-let7a-and-its-mechanism-in-.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ginsenoside Rd on the Biological Characteristics of Lung Adenocarcinoma H1299 Cell Line","authors":"Wei Ding, Xudong Qin, Lingjie Zhou, Zhi-xiang Ming","doi":"10.36468/pharmaceutical-sciences.1331","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1331","url":null,"abstract":"To explore the effect of ginsenoside Rd on the biological characteristics of lung adenocarcinoma H1299 cell line. Firstly, 10 μmol/l ginsenoside Rd, 20 μmol/l ginsenoside Rd, 40 μmol/l ginsenoside Rd, 80 μmol/l ginsenoside Rd, 160 μmol/l ginsenoside Rd were applied to treat H1299 cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay was used to measure the inhibitory rate of glutathione reductase on cell viability, and the half-maximal inhibitory concentration value was calculated as 48.17 μmol/l. After treating H1299 cell line with 48.17 μmol/l ginsenoside Rd for 3 d, effects of ginsenoside Rd on proliferation ability, migration ability, and apoptosis were respectively detected using Ki67 immunofluorescence, Transwell culture system, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay kit. Ginsenoside Rd had inhibitory effects on the viability of H1299 cells, and the inhibition is concentration-dependent. The half-maximal inhibitory concentration value is 131.92 μmol/l; compared to the control group, treatment of H1299 cells with 130 μmol/l ginsenoside Rd induced a significant decrease in Ki67-positive cells number and migratory cells. However, there was hardly any observed apoptosis in both groups. Ginsenoside Rd can suppress activity, proliferation ability, and migration capability of H1299 cell line, which indicates a noticeable suppression of malignancy in H1299 cells. Ginsenoside Rd may have potential therapeutic effects in the treatment of lung adenocarcinoma.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/effect-of-ginsenoside-rd-on-the-biological-characteristics-of-lung-adenocarcinoma-h1299-cell-line.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Plantamajoside on Malignant Behaviors of MHCC97H Cells by Mediating microRNA-3178 Expression","authors":"Xinghua Zhao, Xizhen Li, Mingyu Yang","doi":"10.36468/pharmaceutical-sciences.1337","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1337","url":null,"abstract":"The objective of this study is to explore the effect of plantamajoside on malignant properties of MHCC97H cells and its possible mediating molecular mechanisms. Effects of high, median, and low concentrations of plantamajoside on MHCC97H cell malignant behaviors were assessed. Quantitative reverse transcriptase polymerase chain reaction analysis detected microRNA-3178 expression in tissue samples. Western blotting detected E-cadherin and N-cadherin protein levels. Plantamajoside led to a remarkable reduction in the proliferative, migrating and invasive capabilities of MHCC97H cells, which was accompanied with a diminution in N-cadherin protein levels as well as an augmentation in E-cadherin protein level and microRNA-3178 expression. Reduction of microRNA-3178 was noted in hepatocellular carcinoma samples vs. para-carcinoma tissues. Furthermore, microRNA-3178 overexpression demonstrated an ability to inhibit the effects on the invasion, metastasis, and proliferation of MHCC97H cells. Amusingly, microRNA-3178 silencing attenuated the plantamajoside-mediated inhibitory impact on malignant behaviours of MHCC97H cells. Plantamajoside treatment resulted in an upregulation of microRNA-3178, thus repressing MHCC97H cell invasion, metastasis, and proliferation.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/effect-of-plantamajoside-on-malignant-behaviors-of-mhcc97h-cells-by-mediating-microrna3178-expression.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calycosin Inhibits Pancreatic Cancer Cell Proliferation, Migration and Invasion by Repressing Circ_0000285","authors":"Ruibing Ma, Xiaoping Yu, Kun Cheng","doi":"10.36468/pharmaceutical-sciences.1294","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1294","url":null,"abstract":"Recent studies have shown that calycosin possess multiple pharmacological properties, containing anti-cancer activity. Hence, this project explored the effect and possible mechanism of calycosin in pancreatic cancer. After being cultured, SW1990 cells were exposed to different concentrations of calycosin. 3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyl tetrazolium bromide, plate colony formation, and transwell experiments assessed cell proliferation, colony formation, migration, and invasion. Circ_0000285 expression was assessed using quantitative reverse transcription-polymerase chain reaction experiment. E-cadherin and N-cadherin protein levels were examined using Western blot. After calycosin treatment, SW1990 cell proliferation, migration, invasion, circ_0000285 expression, N-cadherin were repressed, and E-cadherin was increased in dose-dependent way. Circ_0000285 deficiency might hinder cell proliferation, migration, and invasion. Furthermore, the upregulation of circ_0000285 might partly antagonize calycosin-mediated SW1990 cell proliferation, migration, and invasion inhibition. Calycosin treatment might block pancreatic cancer cell development through down-regulating circ_0000285.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/calycosin-inhibits-pancreatic-cancer-cell-proliferation-migration-and-invasion-by-repressing-circ0000285.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Impact of Ginkgo Diterpenoid Lactones on Cognitive Impairment in Cerebrovascular Disease-affected Rats","authors":"Xizi Qiu, Qiang Yao, Cheng Hu, M. He, Tiantian Zhou, W. Tian","doi":"10.36468/pharmaceutical-sciences.1270","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1270","url":null,"abstract":"To examine the potential benefits of Ginkgo biloba diterpene glumine injection as a treatment for cerebral small vascular disease, as well as the effects of the injection on oxidative stress and brain inflammation in cerebral small vascular disease model rats, as well as the cognitive function of these animals. 24 male Sprague-Dawley rats were split into three groups randomly; a diterpene glumine injection group, a model group, and a normal control group, eight rats in each group. The cerebral small vascular disease model was induced, and rats in the experimental group underwent water maze testing to evaluate their behavioral and cognitive functions. Biochemical methods were employed to analyze oxidase activity and malondialdehyde levels in brain tissue. Nitric oxide content and inducible nitric oxide synthase activity were performed in brain tissue. Brain water content was determined using the weightlessness method, and the percentage of brain infarct volume was calculated via 2,3,5-triphenyltetrazolium chloride staining. Pathological alterations in brain tissue and neuronal death were observed through terminal deoxyuridine triphosphate nick end labeling, haemotoxylin and eosin labeling techniques. Following the establishment of the cerebral small vascular disease model, there was a decrease in cognitive function, an increase in the neurological impairment score, an increase in the infarct area and brain water content, an observation of oxidative stress and inflammatory infiltration in the brain tissue, a decrease in synaptic function, and an increase in the rate of neuronal apoptosis. Following diterpene glumine injection administration, the cerebral small vascular disease model rats cognitive function progressively returned, the damage to their neurological system decreased, the extent of their cerebral infarction shrank, the levels of oxidative stress and inflammatory infiltration in the brain improved. More significantly, in rats with cerebral small vascular disease model disease, diterpene glumine injection reduced the pathogenic alterations in brain tissue, synaptic function, and neuronal death. Diterpene glumine injection can improve the cognitive function of cerebral small vascular disease model rats, reduce the damage of nerve function and the size of cerebral infarction, and improve the apoptosis and pathological development of brain neurons in cerebral small vascular disease model rats by reducing brain oxidative stress and inflammatory infiltration.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/exploring-the-impact-of-emginkgoem-diterpenoid-lactones-on-cognitive-impairment-in-cerebrovascular-diseaseaffected-rats.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosponge-An Emerging Nanomaterial in Recent Advancement of Novel Drug Delivery: An Overview and Future Perspectives","authors":"Samit Kumar Nandi, Poulomi Biswas","doi":"10.36468/pharmaceutical-sciences.1290","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1290","url":null,"abstract":"Nanosponge can be considered as an effective carrier among all the novel drug delivery systems based on nanotechnology. It is a newer class of nanoparticulate system having 3-dimensional scaffold structure and nanometric cavity size. Owing to the highly porous nature, nanosponge can offer several benefits like capability to entrap multiple active moieties, loading both hydrophobic and hydrophilic drugs, enhancing the drug solubility followed by promoting the bioavailability of drug molecules, and exhibiting programmable release. These spherical shaped colloidal nanocarriers have the potential to elevate the solubility for poorly soluble drugs through their inclusion and non-inclusion activities. The drugs belonging to Biopharmaceutical Classification System class II and IV are the suitable candidates to be developed as nanosponge formulation for increasing their solubility. Emulsion solvent diffusion method, ultrasound-assisted synthesis, high pressure homogenization etc. are the popular methods for preparation of nanosponge. An extensive variety of additives such as polymers, copolymers, cross-linkers, surfactants are used for releasing the drugs to the target site in a predictable manner through various routes of administration like oral, parenteral, topical. In this review, an overview of nanosponge and its recent advancement in field of drug delivery system along with fu ture perspectives will be discussed.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/nanospongean-emerging-nanomaterial-in-recent-advancement-of-novel-drug-delivery-an-overview-and-future-perspectives.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin Inhibits the Growth of Cancer Associated Fibroblast and Gastric Cancer Cell by Increasing the Expression of Caveolin-1 and Inducing Down-Regulation of Autophagy","authors":"Jing Xing, Huilian Shi, Wenqiang Zhu, Lu Zhang, Yi Xua, Hong Shen","doi":"10.36468/pharmaceutical-sciences.1368","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1368","url":null,"abstract":"Gastric cancer has become the fifth most common cause of cancer-related death worldwide. However, the treatment of gastric cancer remains a challenge. Recently, caveolin-1 has attracted the attention because of its association with cancer-associated fibroblasts that play essential roles in tumor growth. In this study, we established a co-culture model using fibroblasts and gastric cancer cells to mimic tumor microenvironment to investigate the effect of caveolin-1 on the growth of gastric cancer cells. It was found that down-regulation of caveolin-1 in cancer-associated fibroblasts led to cellular autophagy and increased gastric cancer cell growth. This co-culture model promoted the expression of biomarkers of myofibroblasts transformation, including alpha-smooth muscle actin and vimentin. Moreover, quercetin, a natural polyphenolic flavonoid compound which is often used for the treatment of gastric cancer, showed a strong inhibitory effect on gastric cancer cell growth in the co-culture; quercetin unregulated caveolin-1 leading to inhibit myofibroblasts oncogenic transformation both in vitro and in vivo. Quercetin treatment significantly reduced cancer cell migration and invasion, suppressed tumor size and weight. Taken together, our findings have provided new insights on the effect of tumor microenvironment, particularly cancer-associated fibroblast, on tumor cell growth. It has also illustrated the molecular mechanism of quercetin in the treatment of gastric cancer.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/quercetin-inhibits-the-growth-of-cancer-associated-fibroblast-and-gastric-cancer-cell-by-increasing-the-expression-of-ca.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Study on the Inhibition of microRNA-211-5p/ Nuclear Factor Kappa B Pathway by Mitoxantrone Affecting on Gastric Cancer Cells","authors":"Juan Wang, Chao Ye, Lihua Liu, Xin He, Yuan Fang, Junyuan Gu, Yao Xu, Zitong Zheng","doi":"10.36468/pharmaceutical-sciences.1357","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1357","url":null,"abstract":"To investigate the role of mitoxantrone in regulating gastric cancer cell phenotypes and the underlying mechanism. To reveal mitoxantrone-induced effects on gastric cancer cell malignancy, AGS cells were divided into different groups according to various study purposes, including negative control, 5 μmol/l mitoxantrone, 10 μmol/l mitoxantrone, 20 μmol/l mitoxantrone, anti-microRNA-negative control, anti-microRNA-211-5p, 20 μmol/l mitoxantrone+microRNA-negative control, and 20 μmol/l mitoxantrone+microRNA-211-5p groups. AGS cell proliferation was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, while apoptotic rate was quantified via flow cytometry. Bcl-2 associated X-protein, activated caspase-3, phosphorylated-p65 and phosphorylated-IκBα was detected by Western blot for protein expression. Malondialdehyde was detected via colorimetry, nicotinamide adenine dinucleotide phosphate oxidase activity was detected by chemiluminescence, superoxide dismutase activity was measured via colorimetry, and microRNA-211-5p was quantified by reverse transcription-quantitative polymerase chain reaction. AGS cell proliferation, superoxide dismutase activity, as well as microRNA-211-5p expression in mitoxantrone groups (5, 10 and 20 μmol/l) were decreased compared with the negative control group, while the apoptotic rate, Bcl-2-associated X-protein and activated caspase-3 protein expression, malondialdehyde content, and nicotinamide adenine dinucleotide phosphate oxidase activity were increased. Protein expression of phosphorylated-p65 and phosphorylated-IκBα (inhibitor of nuclear factor kappa B) was decreased in the 20 μmol/l mitoxantrone group. The microRNA-211-5p expression, cell proliferation, superoxide dismutase activity, and phosphorylated-p65 and phosphorylated-IκBα protein expression in anti-microRNA-211-5p group were significantly decreased in relative to anti-microRNA-negative control group, while cell apoptosis, Bcl-2-associated X-protein and activated caspase-3 protein expression, malondialdehyde content, and nicotinamide adenine dinucleotide phosphate oxidase activity were increased. The microRNA-211-5p expression, cell proliferation, superoxide dismutase activity, and phosphorylated-p65 and phosphorylated-IκBα protein expression in the 20 μmol/l mitoxantrone+microRNA-211-5p group were higher than in 20 μmol/l mitoxantrone+microRNA-negative control group, while apoptotic, Bcl-2-associated X-protein and activated caspase-3 protein expression, malondialdehyde content, and nicotinamide adenine dinucleotide phosphate oxidase activity were lower than those in the 20 μmol/l mitoxantrone+microRNA-negative control group. Mitoxantrone repressed gastric cancer cell tumor property by downregulating the microRNA-211-5p/nuclear factor kappa B pathway.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/experimental-study-on-the-inhibition-of-microrna2115p-nuclear-factor-kappa-b-pathway-by-mitoxantrone-affecting-on-gastri.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cornus officinalis Extracts Alleviate Amyloid Beta-Peptide 25-35-Induced Nerve Cell Injury in Alzheimer’s Disease by Upregulating Long Non-Coding RNA Rpph1","authors":"Shuhe Zhao, Yanping Duan, Xuan Chen","doi":"10.36468/pharmaceutical-sciences.1360","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1360","url":null,"abstract":"To explore the protective effect of Cornus officinalis extracts on Alzheimer’s disease cell injury models and its underlying molecular mechanism. Pheochromocytoma cells were treated with 20 μM amyloid beta-peptide 25-35 to establish an Alzheimer’s disease cell injury model in vitro, which was recorded as amyloid beta-peptide 25-35 group. Cells affected by amyloid beta-peptide 25-35 were treated with different-doses of Cornus officinalis extracts and recorded as low-dose Cornus officinalis extracts group, medium-dose Cornus officinalis extracts group and high-dose Cornus officinalis extracts group. Pheochromocytoma cells transfected with si-NC/si-long non-coding RNA Rpph1, treated with 20 μM amyloid beta-peptide 25-35 and 80 mg/ml of Cornus officinalis extracts were recorded as high-dose Cornus officinalis extracts+si-NC group, high-dose Cornus officinalis extracts+si-long non-coding RNA Rpph1 group. Cell viability and apoptosis were examined using cell counting kit-8 assay and flow cytometry. Protein expression was tested by Western blot. Malondialdehyde, superoxide dismutase and catalase levels were assessed by measuring cell oxidative stress. Amyloid beta-peptide, tumor necrosis factor-alpha, interleukin-6 and interferon gamma levels were examined using enzyme-linked immunosorbent assay. Long non-coding RNA Rpph1 expression was detected using quantitative reverse transcriptase polymerase chain reaction. Amyloid beta-peptide 25-35 treatment decreased Pheochromocytoma cell viability, CyclinD1, superoxide dismutase, catalase and long non-coding RNA Rpph1 levels, while increased apoptosis rate, cleaved-caspase-3, malondialdehyde, amyloid beta-peptide, tumor necrosis factor-alpha, interleukin-6 and interferon gamma levels. After treatment with different-doses of Cornus officinalis extracts, cell viability, CyclinD1, superoxide dismutase, catalase and long non-coding RNA Rpph1 levels were enhanced, while apoptosis rate, cleaved-caspase-3, malondialdehyde, Aβ, tumor necrosis factor-alpha, interleukin-6 and interferon gamma levels were reduced in pheochromocytoma cells treated with amyloid beta-peptide 25-35. Downregulation of long non-coding RNA Rpph1 reversed the inhibitory effect of Cornus officinalis extracts on cell injury. Cornus officinalis extracts relieved amyloid beta-peptide 25-35-induced nerve cell injury by upregulating long non-coding RNA Rpph1, suggesting that Cornus officinalis extracts might be used in Alzheimer's disease treatment.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/emcornus-officinalisem-extracts-alleviate-amyloid-betapeptide-2535induced-nerve-cell-injury-in-alzheimers-disease-by-upr.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}