Indian Journal of Pharmaceutical Sciences最新文献

{"title":"Resveratrol Ameliorates Lipopolysaccharide-Induced Injury by Regulating Apoptosis and NRF2-Mediated Antioxidant Pathway in HMC3 Cells","authors":"Yousu Shen, Xiaobing Liu, Xianwei Jin, Xia Jin, Chunhui Qin, Mingsheng Zhang, Fen Liu","doi":"10.36468/pharmaceutical-sciences.1348","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1348","url":null,"abstract":"Postoperative cognitive dysfunction is a common complication following anesthesia and surgery, and is associated with oxidative stress and apoptosis in the central nervous system. Resveratrol, an antioxidant, natural polyphenol, has been reported to have neuroprotective effect. The aim of this study was to examine whether resveratrol could attenuate the apoptosis and oxidative stress in a cell model of postoperative cognitive dysfunction. A cell model of postoperative cognitive dysfunction was constructed using human microglial clone 3 cells exposed to 1000 ng/ml lipopolysaccharide, 50 µM resveratrol, and their combination for 48 h. Then, the cell viability, proliferation, apoptosis, production of reactive oxygen species, and the expression of apoptosis-related genes and nuclear factor-E2-related factor 2 and its downstream antioxidant genes were examined. In the postoperative cognitive dysfunction cell model, lipopolysaccharide decreased the cell viability and proliferation, while it increased cell apoptosis and reactive oxygen species level. Lipopolysaccharide also upregulated the expression of pro-apoptotic genes (Bcl-2-associated X-protein and caspase-3) and genes involved in nuclear factor-E2-related factor 2-mediated antioxidant pathway (nuclear factor-E2-related factor 2, Kelch-like ECH-associated protein 1, heme oxygenase-1, and superoxide dismutase-2) in human microglial clone 3 cells. Resveratrol ameliorated the lipopolysaccharide-induced decrease of cell viability and proliferation, increase of cell apoptosis and reactive oxygen species level, and upregulation of the expression of pro-apoptotic genes. Additionally, resveratrol upregulated the expression of nuclear factor-E2-related factor 2 and its downstream antioxidant genes in lipopolysaccharide-treated human microglial clone 3 cells. Resveratrol protects human microglial clone 3 cells against lipopolysaccharide-induced injury by reducing oxidative stress through activating nuclear factor-E2-related factor 2-mediated antioxidant pathway.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/resveratrol-ameliorates-lipopolysaccharideinduced-injury-by-regulating-apoptosis-and-nrf2mediated-antioxidant-pathway-in.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iridoid Glucosides from Boschniakia rossica Inhibits Ovarian Cancer Cell Proliferation and Metastasis through Circular-Homeodomain-Interacting Protein Kinase 3/ microRNA-495-3p Axis","authors":"Ying Chen, Xia Guangming, Jin Xia","doi":"10.36468/pharmaceutical-sciences.1349","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1349","url":null,"abstract":"To explore the effect and possible mechanism of iridoid glucosides from Boschniakia rossica on ovarian cancer cell proliferation and metastasis. Human ovarian cancer cells (OVCAR3) were treated with different concentrations of iridoid glucosides from Boschniakia rossica. Also, human ovarian cancer cells were transfected with plasmid cloning deoxyribonucleic acid, plasmid cloning deoxyribonucleic acid circular-homeodomain-interacting protein kinase 3, microRNA-NC+plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 and microRNA-495-3p mimics+plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3, followed by treated with high-dose of iridoid glucosides from Boschniakia rossica. Cell functions were tested by colony formation, transwell and 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide assays. Gene levels were examined using quantitative reverse transcription polymerase chain reaction and Western blot method. Ribonuclic acid interaction was verified using dual-luciferase reporter assay. Iridoid glucosides from Boschniakia rossica could reduce OVCAR3 cell proliferation and metastasis. Moreover, iridoid glucosides from Boschniakia rossica decreased circular-homeodomain-interacting protein kinase 3 expression and increased microRNA-495-3p expression. Plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 reversed iridoid glucosides from Boschniakia rossica-mediated the inhibition on OVCAR3 cell functions. Circular-homeodomain-interacting protein kinase 3 targeted microRNA-495-3p. Also, microRNA-495-3p mimics abolished the effects of plasmid cloning deoxyribonucleic acid-circular-homeodomain-interacting protein kinase 3 on iridoid glucosides from Boschniakia rossica-mediated cell functions. Iridoid glucosides from Boschniakia rossica inhibited ovarian cancer cell proliferation and metastasis by circular-homeodomain-interacting protein kinase 3/microRNA-495-3p.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/iridoid-glucosides-from-emboschniakia-rossicaem-inhibits-ovarian-cancer-cell-proliferation-and-metastasis-through-circul.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147918348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Houttuyn Berberine Promotes Apoptosis of Nasopharyngeal Carcinoma Cells and Inhibits Proliferation, Migration and Invasion by Regulating the circ-SMARCA5/microRNA-346 pathway","authors":"Dongbao Yang, Xiaodong Wen, Jing Xiao, Hong Qin, Jie Hou, Yujie Li, Xiaojun Liang, F. L. Qiao","doi":"10.36468/pharmaceutical-sciences.1295","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1295","url":null,"abstract":"To explore the effect of houttuyn berberine on the biological behavior of nasopharyngeal carcinoma cells by regulating circ-SMARCA5/microRNA-346 pathway. Nasopharyngeal carcinoma cells C666-1 were divided into control group, houttuyn berberine (1, 2, 4 μg/ml) group, plasmid cloning deoxyribonucleic acid group-circSMARCA5 group, houttuyn berberine+si-circ-SMARCA5 group, and houttuyn berberine+microRNA-346 group. Real-time quantitative polymerase chain reaction detected the expression levels of circ-SMARCA5 and microRNA-346. Cell counting kit, plate cloning experiment, flow cytometry, wound healing assays and Transwell assays were performed to assess the cell viability, clone formation, apoptosis, migration and invasion ability of C666-1. The targeting relationship between circ-SMARCA5 and microRNA-346 was verified with the aid of dual luciferase experiment. In comparison with the control group, the cell viability of C666-1 cells, microRNA-346 expression, wound healing rate, clone formation numbers, invasive numbers in the houttuyn berberine (1, 2, 4 μg/ml) group were increased, and the expression of circ-SMARCA5 and apoptosis rate were increased (p<0.05). Compared with plasmid cloning deoxyribonucleic acid group, the cell viability of C666-1 cells, wound healing rate, microRNA-346 expression in the plasmid cloning deoxyribonucleic acidcirc-SMARCA5 group were reduced, the apoptosis rate was increased, invasive numbers and clone formation numbers were reduced (p<0.05). Circ-SMARCA5 directly bound to microRNA-346. Compared with the houttuyn berberine group, the cell viability of C666-1 cells, clone formation numbers and invasive numbers were increased in the houttuyn berberine+si-circ-SMARCA5 group and houttuyn berberine+microRNA-346 group, the apoptotic rate was decreased, and the wound healing rate was increased (p<0.05). Houttuyn berberine could inhibit nasopharyngeal carcinoma cells’ malignant proliferation, migration and invasion, and induce cell apoptosis. The mechanism is associated with the up-regulation of circ-SMARCA5/microRNA-346 pathway.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/houttuyn-berberine-promotes-apoptosis-of-nasopharyngeal-carcinoma-cells-and-inhibits-proliferation-migration-and-invasio.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Angiotensin II-Mediated Janus Kinase/Signal Transducers and Activators of Transcription Pathways on Thoracic Aortic Smooth Muscle Cells","authors":"Ru Zhang, Zhong Lu, Zhongya Yan","doi":"10.36468/pharmaceutical-sciences.1306","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1306","url":null,"abstract":"At present, the effect of angiotensin II on the biological behavior of smooth muscle cells of thoracic aortic aneurysm is not clear. In this study, thoracic aortic aneurysm rat model (thoracic aortic aneurysm model group) was prepared by applying calcium chloride to blood vessels, and normal rats were taken as a sham operation group (sham group), with 10 rats in each group. The changes of angiotensin II content in plasma and left ventricular myocardial tissue were detected. Normal rat thoracic aortic smooth muscle cells were extracted and divided into the control group, angiotensin II group (1 μg/ml angiotensin II), and angiotensin II+angiotensin receptor blocker group (1×10-6 mol/l candesartan+1 μg/ml angiotensin II). The cholecystokinin cell counting kit-8, flow cytometry, Transwell chamber, scratch healing, and Western blot experiments were used to detect cell proliferation, apoptosis, invasion, migration, and the expression changes of Janus kinase/signal transducers and activators of transcription pathway-related proteins. The results showed that angiotensin II content in left ventricular myocardium of thoracic aortic aneurysm group was higher than that of Sham group (p<0.01). Compared with the control group, angiotensin II group showed increased cell proliferative activity, number of invaded cells, mobility, and expression levels of Ras-related C3 botulinum toxin substrate 1, phosphorylated-Janus kinase 2, phosphorylated-signal transducers and activators of transcription 1, and phosphorylated-signal transducers and activators of transcription 3 proteins, and decreased apoptosis rate (p<0.05). Compared with the angiotensin II group, the cell proliferative activity, the number of invaded cells, and mobility as well as the expression levels of Ras-related C3 botulinum toxin substrate 1, phosphorylated- Janus kinase 2, phosphorylated-signal transducers and activators of transcription 1, and phosphorylatedsignal transducers and activators of transcription 3 proteins in the angiotensin II+angiotensin receptor blocker group were decreased, and the apoptosis rate was increased (p<0.05). These results indicated that angiotensin II could induce the proliferation, invasion, and migration of thoracic aortic aneurysm smooth muscle cells, and promote apoptosis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/effect-of-angiotensin-iimediated-janus-kinasesignal-transducers-and-activators-of-transcription-pathways-on-thoracic-aor.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ailanthone Suppresses Liver Cancer Cell Malignant Behavior by Regulating microRNA-489-3p","authors":"Ruiyu Guan, Yiyang Yao, Zengliang Liang","doi":"10.36468/pharmaceutical-sciences.1258","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1258","url":null,"abstract":"Previous literature has validated the clinical effectiveness of ailanthone in repressing the development of different human cancers. Therefore, this project aimed to investigate the role and possible mechanism of ailanthone in liver cancer. Huh-7 cells were treated with various concentrations of ailanthone (low-dose ailanthone, medium-dose ailanthone, high-dose ailanthone), or transfected with microRNA-negative control and microRNA-489-3p mimics. Furthermore, transfected Huh-7 cells were exposed with high-dose ailanthone. Cell counting kit-8, plate clone formation, scratch, and Transwell detected cell proliferation, clone formation, migration, and invasion. Besides, microRNA-489-3p, matrix metalloproteinase-2, and matrix metalloproteinase-9 contents were assessed using quantitative reverse transcription polymerase chain reaction or Western blot. After exposure to various doses of ailanthone, cell proliferation inhibition rate and microRNA-489-3p expression were increased in a dose-dependent manner, whereas cell clone formation, migration, invasive, and matrix metalloproteinase-2, matrix metalloproteinase-9 protein levels were decreased. Beyond that, microRNA-489- 3p expression was reduced in liver cancer tissues; its upregulation might block Huh-7 cell growth. MicroRNA489-3p downregulation might abrogate high-dose ailanthone-mediated cell malignant behaviors inhibition. Ailanthone treatment could hinder Huh-7 cell malignant behaviors through regulating microRNA-489-3p.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/ailanthone-suppresses-liver-cancer-cell-malignant-behavior-by-regulating-microrna4893p.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huangqi Decoction Serum Suppressed Proliferation and Migration of Rat Liver Sinusoidal Endothelial Cells via Angiogenesis Inhibition by Promoting AKT/mTOR-Dependent Autophagy","authors":"Changyan Wan, Tianlu Hou, Cheng Yang","doi":"10.36468/pharmaceutical-sciences.1364","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1364","url":null,"abstract":"Huangqi decoction, one of the traditional Chinese medicine prescriptions, has been found useful for the treatment of liver fibrosis. This study focused on investigating Huangqi decoction serum effect on growth, migration and angiogenesis of rat liver sinusoidal endothelial cells. We administered Huangqi decoction (20 mg/100 g) into Sprague-Dawley rats orally once daily for 3 d consecutively. Then, Huangqi decoction serum of rats was obtained. Initial Huangqi decoction serum was considered a high concentration. The initial Huangqi decoction serum was diluted one time and three times respectively to create a medium and low concentration. Liver sinusoidal endothelial cells were isolated from Sprague-Dawley rats, and the Huangqi decoction serum was obtained. Liver sinusoidal endothelial cells without any treatment were used as control. Liver sinusoidal endothelial cells treated by vascular endothelial growth factor A (40 ng/ml) served as vascular endothelial growth factor A group. Huangqi decoction serum hindered protein kinase B and mammalian target of rapamycin protein phosphorylation and microtubule-associated protein light chain 3 II/microtubule-associated protein light chain 3 I and Beclin-1 protein expression in rat liver sinusoidal endothelial cells (p<0.01). SC79 and 3MA treatment reversed high-concentration of Huangqi decoction serum inhibition on liver sinusoidal endothelial cells proliferation, migration, angiogenesis, and promotion on autophagy in rat liver sinusoidal endothelial cells (p<0.01). Huangqi decoction serum hindered proliferation and migration of rat liver sinusoidal endothelial cells via angiogenesis inhibition by promoting protein kinase B/mammalian target of rapamycin-dependent autophagy. Huangqi decoction might be applied to be an effective drug to treat patients' liver fibrosis in the future.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/huangqi-decoction-serum-suppressed-proliferation-and-migration-of-rat-liver-sinusoidal-endothelial-cells-emviaem-angioge.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radix Ranunculus ternatus Saponins Inhibits Malignant Behavior in Sarcoma Osteogenic-2 Osteosarcoma Cells Through microRNA-1305 Upregulation","authors":"Xiaobin Lin, Jianxin Huang, Hui Ye, Xiaocong Lin","doi":"10.36468/pharmaceutical-sciences.1338","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1338","url":null,"abstract":"To investigate the effects of radix Ranunculus ternatus saponins on sarcoma osteogenic-2 cell malignancy and its possible mechanism. Sarcoma osteogenic-2 cells were treated with different doses (50, 100, 200 mg/l) of radix Ranunculus ternatus saponins for 24 h. Effect of radix Ranunculus ternatus saponins on sarcoma osteogenic-2 cell malignant behaviors were detected by cell counting, colony formation, wound-healing, and transwell invasion assays. Protein levels were detected by Western blotting. Treatment with radix Ranunculus ternatus saponins repressed the proliferative, migratory, and invading capacities of sarcoma osteogenic-2 cells in a dose-dependent manner. Radix Ranunculus ternatus saponins reduced neural-cadherin protein levels and elevated epithelial-cadherin protein levels. MicroRNA-1305 was downregulated in osteosarcoma samples, while microRNA-1305 was elevated in microRNA-1305 treated sarcoma osteogenic-2 cells. Upregulation of microRNA-1305 restrained sarcoma osteogenic-2 cell proliferation, metastasis, and invasion, while microRNA-1305 knockdown radix Ranunculus ternatus saponins-mediated inhibiting effects on sarcoma osteogenic-2 cell malignant behaviors. Radix Ranunculus ternatus saponins might inhibit sarcoma osteogenic-2 cell malignant behaviors by up-regulating miR-1305, which has a potential value for osteosarcoma treatment.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/radix-emranunculus-ternatusem-saponins-inhibits-malignant-behavior-in-sarcoma-osteogenic2-osteosarcoma-cells-through-mic.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin Promotes the Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Up-Regulating microRNA-148a","authors":"Junling Shan, Hui-Feng Wang, Guangyu Zhu, Wenjing Zhao","doi":"10.36468/pharmaceutical-sciences.1256","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1256","url":null,"abstract":"To probe curcumin action on the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells and its possible molecular mechanism. Human bone marrow mesenchymal stem cells were osteogenic induced for 14 d. Real-time quantitative reverse transcription-polymerase chain reaction and Western blotting analysis were utilized for the detection of microRNA-148a, alkaline phosphatase, osteocalcin and osteopontin levels in human bone marrow mesenchymal stem cells after curcumin incubation for 14 d. After curcumin treatment, the proliferation of human bone marrow mesenchymal stem cells was notably rose, and contents of alkaline phosphatase, osteocalcin and osteopontin in osteogenic induced-human bone marrow mesenchymal stem cells were elevated. Curcumin treatment elevated microRNA-148a expression. Overexpression of microRNA-148a could boost human bone marrow mesenchymal stem cells proliferation, and increased alkaline phosphatase, osteocalcin and osteopontin contents in human bone marrow mesenchymal stem cells during osteogenesis induction. Down-regulation of microRNA-148a reversed the promoting effect of curcumin on human bone marrow mesenchymal stem cells proliferation and the levels of alkaline phosphatase, osteocalcin and osteopontin in human bone marrow mesenchymal stem cells during osteogenesis induction (p<0.05). Curcumin promoted the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells by up-regulating microRNA-148a.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/curcumin-promotes-the-proliferation-and-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-by-upregulating.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Emodin on Inflammatory Factors and Cellular Apoptosis in Knee Osteoarthritis, Focusing on the High Mobility Group Box-1/Toll-Like Receptor 4 Signaling Pathway","authors":"Haibo Li, Jinyang Zhao, Jinke Sun, Xu Wang","doi":"10.36468/pharmaceutical-sciences.1358","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1358","url":null,"abstract":"Using both cellular and animal models, this research assessed emodin effect on human chondrocytes as well as its underlying anti-inflammatory mechanisms. The results demonstrated that emodin had no significant impact on cell viability in normal chondrocytes, but exhibited a dose-dependent protective effect on lipopolysaccharides-induced inflammatory chondrocytes and inhibition of cell apoptosis. Moreover, emodin was found to reduce the levels of inflammatory cytokines induced by lipopolysaccharides. Mechanistically, emodin decreased the levels of reactive oxygen species in lipopolysaccharides-treated TC28a2 human chondrocyte cells and hampered the stimulation of the high mobility group box-1/toll-like receptor 4/nuclear factor kappa B signaling pathway. In a knee osteoarthritis rat model, emodin improved the morphology of cartilage tissue, suppressed cell apoptosis, and restrained the high mobility group box-1/toll-like receptor 4/nuclear factor kappa B signaling pathway and reactive oxygen species accumulation. In summary, emodin exhibited an anti-inflammatory effect, improving cell survival, suppressing apoptosis in chondrocytes, as well as reducing the levels of inflammatory cytokines, which might be achieved by modulating the high mobility group box-1/toll-like receptor 4/nuclear factor kappa B signaling pathway and reactive oxygen species levels. These results indicate that emodin seems to have therapeutic value in the treatment of knee osteoarthritis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/impact-of-emodin-on-inflammatory-factors-and-cellular-apoptosis-in-knee-osteoarthritis-focusing-on-the-high-mobility-gro.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allium mongolicum Regel Flavonoids Alleviate Hypoxia/ Reoxygenation-Induced Cardiomyocyte Injury by Downregulating lncRNA TALNEC2","authors":"Huaming Zhang, Feng Xi","doi":"10.36468/pharmaceutical-sciences.1273","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1273","url":null,"abstract":"To explore the effect and possible mechanism of Allium mongolicum Regel flavonoids on cardiomyocyte injury induced by hypoxia/reoxygenation. H9C2 cardiomyocytes were induced by hypoxia/reoxygenation and treated with different doses of Allium mongolicum Regel flavonoids. H9C2 cells were transfected with si-negative control/ si-TALNEC2 and treated with hypoxia/reoxygenation. Besides, cells transfected with plasmid cloning deoxyribonucleic acid/plasmid cloning deoxyribonucleic acid-TALNEC2 were treated with Allium mongolicum Regel flavonoids and induced with hypoxia/reoxygenation. Malondialdehyde, glutathione peroxidase and superoxide dismutase levels were tested to evaluate oxidative stress. Apoptosis rate was analyzed by flow cytometry. TALNEC2 expression was examined using quantitative reverse transcription-polymerase chain reaction, and cleaved caspase-3 and cleaved caspase-9 protein levels were tested by Western blot. Allium mongolicum Regel flavonoids could reduce malondialdehyde level, apoptosis rate, cleaved caspase-3 level, cleaved caspase-9 level, and TALNEC2 expression, while enhanced glutathione peroxidase and superoxide dismutase levels in hypoxia/reoxygenation-induced H9C2 cells in a dose-dependent manner. After transfection of si-TALNEC2, malondialdehyde level, apoptosis rate, cleaved caspase-3 level, and cleaved caspase-9 level were reduced, while superoxide dismutase and glutathione peroxidase levels were enhanced. Transfection of plasmid cloning deoxyribonucleic acid-TALNEC2 could abolish the effect of Allium mongolicum Regel flavonoids on cardiomyocyte injury. Allium mongolicum Regel flavonoids could inhibit hypoxia/reoxygenation-induced cardiomyocyte apoptosis and oxidative stress via reducing TALNEC2 expression.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/emallium-mongolicumem-regel-flavonoids-alleviate-hypoxia-reoxygenationinduced-cardiomyocyte-injury-by-downregulating-lnc.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}