Indian Journal of Pharmaceutical Sciences最新文献

{"title":"Role of Deubiquitinase Josephin Domain Containing 2 in Proliferation and Migration of Human Hepatoma Cells Through Suppression of Dual Specificity Phosphatase 28 Ubiquitylation","authors":"Qinying Feng, Xiaoyu Song, Li Liu, Xinzhong Zhou, Zhihao Chen","doi":"10.36468/pharmaceutical-sciences.1428","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1428","url":null,"abstract":"This study aims to uncover the regulatory mechanism behind the up-regulation of dual-specificity phosphatase 28 in hepatocellular carcinoma. The differential expression and survival analyses of studied genes were carried out by the Encyclopedia of Ribonucleic Acid Interactomes platform. The GeneMANIA and biological general repository for interaction datasets were applied to obtain the regulatory network of dual-specificity phosphatase 28. Western blot analyzed the protein expression levels of dual-specificity phosphatase 28 and Josephin domain containing 2 in clinical samples collected from hepatocellular carcinoma individuals. To study the functions of dual-specificity phosphatase 28 and Josephin domain containing 2, transfection was performed in hepatocellular carcinoma cells, of which efficiency was determined by quantitative real-time polymerase chain reaction and Western blot. Cell proliferation was evaluated by colony formation and ethynyl deoxyuridine assays. Both dual-specificity phosphatase 28 and Josephin domain containing 2 were found to be highly expressed in hepatocellular carcinoma. The overexpression of dual-specificity phosphatase 28 exerts a promotion role in the cell proliferation and migration of human hepatocellular carcinoma cells, whereas the deletion of dual-specificity phosphatase 28 exhibited the opposite role. Bioinformatics tools found that Josephin domain containing 2 is an interacted gene of dual-specificity phosphatase 28, which was verified by co-immunoprecipitation. Josephin domain containing 2 regulates dual-specificity phosphatase 28 stability through deubiquitination. Furthermore, overexpressing dual-specificity phosphatase 28 reversed both the proliferation and migration of hepatocellular carcinoma cells inhibited by Josephin domain containing 2 silencing. Josephin domain containing 2 interacts with and deubiquitinates dual-specificity phosphatase 28 to improve its protein stability, thereby promoting the proliferation and migration of hepatocellular carcinoma cells.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/role-of-deubiquitinase-josephin-domain-containing-2-in-proliferation-and-migration-of-human-hepatoma-cells-through-suppr.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total Flavonoids of Eucommia ulmoides Oliver Protects Cardiomyocytes against Lipopolysaccharide-Induced Injury by Regulating microRNA-494 Expression","authors":"Chen Sun, Xueli Ren, Feini Wu, Wei Chen, Jing Wang, Lili Deng","doi":"10.36468/pharmaceutical-sciences.1300","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1300","url":null,"abstract":"To explore the protective mechanism of total flavonoids from Eucommia ulmoides Oliver leaves (thin-film electroluminescent) on lipopolysaccharide-induced cardiomyocyte H9C2 injury. Cardiomyocytes were divided into control, lipopolysaccharides, lipopolysaccharides+thin-film electroluminescent-low group, lipopolysaccharides+thin-film electroluminescent-middle, lipopolysaccharides+thin-film electroluminescent-high, lipopolysaccharides+microRNA-negative control, lipopolysaccharides+microRNA-494, lipopolysaccharides+thin-film electroluminescent+anti-microRNA-negative control, and lipopolysaccharides+thin-film electroluminescent+anti-microRNA-494 groups. The commercial kits were used to evaluate superoxide dismutase and glutathione peroxidase activities, as well as malondialdehyde levels. Flow cytometry was utilized for the estimation of cell apoptosis. Western blotting was employed to detect cleaved caspase-3 and cleaved caspase-9 protein levels. Reverse transcription-quantitative polymerase chain reaction was selected to detect microRNA-494 expression. Lipopolysaccharides significantly decreased malondialdehyde levels, increased glutathione peroxidase and superoxide dismutase activities, induced cell apoptosis, and upregulated cleaved caspase-3 and cleaved caspase-9 protein levels in cardiomyocytes, suggesting that lipopolysaccharides facilitated cardiomyocyte oxidative stress and apoptosis. Thin-film electroluminescent overtly weakened lipopolysaccharides induced cardiomyocyte oxidative stress and apoptosis in a dose-dependent mode. Thin-film electroluminescent partly overturned lipopolysaccharides induced downregulation of microRNA-494 expression in cardiomyocytes. Furthermore, elevation of microRNA-494 impaired lipopolysaccharides-induced cardiomyocyte oxidative stress and apoptosis. In addition, microRNA-494 knockdown weakened thin-film electroluminescent mediated repressive effects on lipopolysaccharides induced cardiomyocyte oxidative stress and apoptosis. Thin-film electroluminescent could alleviate lipopolysaccharides induced cardiomyocyte oxidative stress and apoptosis, which was achieved by upregulating microRNA-494 expression.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/total-flavonoids-of-emeucommia-ulmoidesem-oliver-protects-cardiomyocytes-against-lipopolysaccharideinduced-injury-by-reg.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Expression of Nuclear Factor-Kappa B Signaling Pathway and Apoptosis of Renal Tubular Epithelial Cells in Rats with Calcium Oxalate Kidney Stones","authors":"Xiaowei Zheng, Shaohua Chen, Xiaozhi Yao, Yun Chen","doi":"10.36468/pharmaceutical-sciences.1287","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1287","url":null,"abstract":"The study aimed to explore the effect of the nuclear factor-kappa B signaling pathway on apoptosis of rat renal tubular epithelial cells caused by calcium oxalate monohydrate crystals. After being cultivated in vitro, NRK-52E cells were split into a control group, 1 mm calcium oxalate monohydrate group and 10 μM QNZ+1 mm calcium oxalate monohydrate group. The optical density value was detected via cell counting kit-8 assay, the content of lactate dehydrogenase in cell supernatant was detected via colorimetry, and the expressions of nuclear factor-kappa B signaling pathway-related proteins p65 and p50 and B cell lymphoma-2, tumor necrosis factor receptor 1 and caspase-3 were determined through Western blotting. 1 mm calcium oxalate monohydrate could significantly reduce the proliferation ability of NRK-52E cell and cause cytotoxicity. 1 mm calcium oxalate monohydrate significantly up-regulated the expressions of p65, p50, tumor necrosis factor receptor 1 and caspase-3, and significantly lowered the B cell lymphoma-2. Besides, 10 μM QNZ+1 mm calcium oxalate monohydrate obviously down-regulated the expressions of p65, p50, tumor necrosis factor receptor 1 and caspase-3, and obviously increased the B cell lymphoma. The apoptosis of rat renal tubular epithelial cells in rats with calcium oxalate kidney stones may be promoted through activating the nuclear factor-kappa B signaling pathway. Inhibiting the nuclear factor-kappa B activity can alleviate apoptosis and improve renal cell function.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/association-between-expression-of-nuclear-factorkappa-b-signaling-pathway-and-apoptosis-of-renal-tubular-epithelial-cell.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kifunensine Attenuates Transforming Growth Factor-Beta 1 Induced Cardiac Fibrosis by Inhibiting Transforming Growth Factor-Beta Receptor Complex Formation","authors":"Juanjuan Zhang, Linna Zhao, Yangong Liu, Gaoshan Yang, Yi-Ping Qin, Weizhe Liu, Aiying Li","doi":"10.36468/pharmaceutical-sciences.1410","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1410","url":null,"abstract":"N-glycosylation, the predominant post-translational protein modification, significantly influences protein physicochemical properties and biological functions. Kifunensine, a N-glycosylation inhibitor, shows promise as a potential therapeutic agent for modulating the hyperactivation of transforming growth factor-beta 1 signalling pathways in fibrotic diseases. This study explored kifunensine's effects and mechanisms of action using an innovative in vitro cardiac fibrosis model involving primary rat cardiac fibroblasts treated with transforming growth factor-beta 1. Protein expression related to cardiac fibrosis was assessed via Western blotting and immunofluorescence assays. Kifunensine significantly protected these cells against transforming growth factor-beta 1 induced fibrosis, as indicated by decreased collagen I, collagen III, and alpha-smooth muscle actin expression. Interestingly, kifunensine did not alter transforming growth factor-beta receptor expression but reduced transforming growth factor-beta 1 ligand-receptor complex formation, inhibiting transforming growth factor-beta type I receptor and transforming growth factor-beta type II receptor activation and suppressing suppressor of mothers against decapentaplegic 2 and suppressor of mothers against decapentaplegic 3 phosphorylation. This underlines N-glycosylation's role in cardiac fibrosis and establishes the anti-fibrotic potential of moderate N-glycan alteration on transforming growth factor-beta receptors using kifunensine, suggesting a novel therapeutic strategy for myocardial fibrosis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/kifunensine-attenuates-transforming-growth-factorbeta-1-induced-cardiac-fibrosis-by-inhibiting-transforming-growth-facto.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platycodin D Impairs the Oncogenic Phenotypes of Esophageal Squamous Cell Carcinoma Cells by Elevating microRNA-4319","authors":"Shuo Zhang, Yinghua Ma, Geng Xiao","doi":"10.36468/pharmaceutical-sciences.1311","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1311","url":null,"abstract":"To probe the action of platycodin D in the oncogenic phenotypes of esophageal squamous cell carcinoma cells and its possible mechanism. Esophageal squamous cell carcinoma cell line EC9706 was exposed to different doses of platycodin D (2.5, 5 or 10 μmol/l). Untreated cells were adopted as control group. After indicated transfection, functional analyses were carried out. The proliferation, migration and invasion of EC9706 cells were dose-dependently decreased by platycodin D. Moreover, platycodin D treatment dose-dependently increased microRNA-4319 levels in cells. MicroRNA-4319 rise impaired EC9706 cell proliferation, invasiveness and migration. Besides that, the inhibition of microRNA-4319 abolished the action of platycodin D in EC9706 cells. Platycodin D could impair cell proliferation, migration and invasion abilities in esophageal squamous cell carcinoma through elevating microRNA-4319.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/platycodin-d-impairs-the-oncogenic-phenotypes-of-esophageal-squamous-cell-carcinoma-cells-by-elevating-microrna4319.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese Herbal Medicine Jia Wei Jing Xie Yin Ameliorates Imiquimod-Induced Psoriasis by Modulating Gamma Delta T Cells","authors":"Jie Wu, Yu Lu, Deng‐Ke Yang, Wen-Lei Zhao, Yiyu Guo, Shaodan Zhang, Lanlan Huang, Jianping Qin, Yan Xiao, Xinrui Tang, Xuanran Liang","doi":"10.36468/pharmaceutical-sciences.1271","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1271","url":null,"abstract":"Psoriasis is a chronic immune-mediated inflammatory skin disease in which gamma delta T cells play an important role. Chinese herbal medicine Jia Wei Jing Xie Yin has its origin in Jing Xie Yin, a medicine created by the traditional Chinese medicine doctor Wu Jun. Previous studies have shown that Jia Wei Jing Xie Yin has good clinical efficacy for patients with blood-heat type psoriasis by inhibiting the interleukin-23/T-helper 17 immune inflammation axis. This study aims to determine the immunoregulatory effects and the underlying mechanisms of Jia Wei Jing Xie Yin in psoriasis-related inflammatory responses. Bagg albino mice with imiquimod-induced psoriasis-like dermatitis were treated with saline (Model), methotrexate that serves as a positive control, or low, medium and high doses of Jia Wei Jing Xie Yin intragastrically. The pathological changes, inflammatory cell infiltration, the expression of inflammatory cytokines, and Janus tyrosine kinase 3/signal transducers and activators of transcription pathway related proteins in the skin lesion were examined. The abundance of Vγ4+ T cells in lymph nodes and spleen was determined by flow cytometry. Jia Wei Jing Xie Yin ameliorated psoriasis area and severity index scores, reduced the infiltration of inflammatory cells and interleukin-17 producing gamma delta T cells, inhibited the messenger ribonucleic acid expression of interleukin-17, interleukin-22, interleukin-6, tumour necrosis factor alpha and matrix metalloproteinase-9, reduced inflammatory cell infiltration and pathological damage. Nevertheless, the expression of the Janus tyrosine kinase 3/signal transducers and activators of transcription signaling pathway was not significantly changed in the MTX and Jia Wei Jing Xie Yin groups. Our work demonstrates that Chinese herbal medicine Jia Wei Jing Xie Yin alleviates Imiquimod-induced inflammation in psoriasis. We have found a good regulatory effect of Jia Wei Jing Xie Yin on gamma delta T cells.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/chinese-herbal-medicine-jia-wei-jing-xie-yin-ameliorates-imiquimodinduced-psoriasis-by-modulating-gamma-delta-t-cells.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marsdenia tenacissima Extract Affects the Proliferation, Apoptosis, and Migration of Oral Squamous Cell Carcinoma Cells Through hsa_circular_0003645/microRNA-335","authors":"Lixin Wang, Jin Fang","doi":"10.36468/pharmaceutical-sciences.1344","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1344","url":null,"abstract":"Numerous papers stated that Marsdenia tenacissima extract possessed tumor-suppression properties. Herein, we aimed to investigate the influence and underlying mechanism of Marsdenia tenacissima extract on oral squamous cell carcinoma. After being cultured, CAL-27 cells were treated with various doses of Marsdenia tenacissima extract. Proliferation, apoptosis, and migration were assessed using cell counting kit-8, clone formation, scratch, and flow cytometry assays. Western blot detected protein levels. Quantitative reverse transcription polymerase chain reaction method analyzed hsa_circular_0003645 and microRNA-335 expression. Their interaction was validated using dual-luciferase reporter gene experiment. 1.0 and 1.5 mg/ml Marsdenia tenacissima extract or si-hsa_circular_0003645 could suppress CAL-27 cell proliferation, migration, N-cadherin, and hsa_circ_0003645, whereas increase apoptosis, E-cadherin, and microRNA-335. Furthermore, the upregulation of hsa_circular_0003645 might abolish Marsdenia tenacissima extract exposure-mediated oral squamous cell carcinoma cell proliferation and migration inhibition and apoptosis promotion. Besides, hsa_circular_0003645 could target microRNA-335. Marsdenia tenacissima extract treatment might hinder oral squamous cell carcinoma progression via regulating hsa_circular_0003645 and microRNA-335.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/emmarsdenia-tenacissimaem-extract-affects-the-proliferation-apoptosis-and-migration-of-oral-squamous-cell-carcinoma-cell.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147912239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleuropein Inhibits Astrocyte Damage Induced by Oxygen-Glucose Deprivation","authors":"Shihang Zhang, Yao Fu, Mojiao Zhao, Xue Li, Na Zhao","doi":"10.36468/pharmaceutical-sciences.1275","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1275","url":null,"abstract":"To investigate the effect and its mechanism of oleuropein on the oxidative damage and apoptosis of astrocytes induced by oxygen-glucose deprivation, and its effect on circ_TTC3 and microRNA-138-5p. Astrocytes were divided into control group, oxygen-glucose deprivation group, oxygen-glucose deprivation+oleuropein 25 μmol/l, 50 μmol/l, 100 μmol/l groups, oxygen-glucose deprivation+si-negative control, si-circ_TTC3 group, oxygen-glucose deprivation+oleuropein+plasmid cloning deoxyribonucleic acid plasmid cloning deoxyribonucleic acid and plasmid cloning deoxyribonucleic acid-circ_TTC3 group. In comparison with the control group, the inhibiting rate of cell growth, apoptosis rate, malondialdehyde, lactate dehydrogenase levels and circ_TTC3 expression of astrocytes in the oxygen-glucose deprivation group were increased, while superoxide dismutase activity and miR-138-5p expression were declined (p<0.05). Compared to the oxygen-glucose deprivation group, the proliferative inhibiting rate, apoptosis rate, malondialdehyde, lactate dehydrogenase levels and circ_TTC3 expression of astrocytes in the oxygen-glucose deprivation+oleuropein 25 μmol/l group, oxygen-glucose deprivation+oleuropein 50 μmol/l group, and oxygen-glucose deprivation+oleuropein 100 μmol/l group were all weakened, superoxide dismutase activity and microRNA-138-5p expression were enhanced (p<0.05), and all were concentration-dependent. After silencing circ_TTC3, circ_TTC3 expression, malondialdehyde, lactate dehydrogenase levels, cell proliferation inhibition rate and apoptosis rate in astrocytes in the oxygen-glucose deprivation+si-circ_TTC3 group were all lower than those in oxygen-glucose deprivation+si-negative control group, while the superoxide dismutase activity was higher than that in oxygen-glucose deprivation+si-negative control group (p<0.05). Circ_TTC3 targeted and regulated microRNA-138-5p. Circ_TTC3 level, malondialdehyde and lactate dehydrogenase levels, cell proliferation inhibition rate, and apoptosis rate in astrocytes in the oxygen-glucose deprivation+oleuropein+ plasmid cloning deoxyribonucleic acid-circ_TTC3 group were all higher than those in oxygen-glucose deprivation+oleuropein+plasmid cloning deoxyribonucleic acid group, and there was a decrease of superoxide dismutase activity in the oxygen-glucose deprivation +oleuropein+plasmid cloning deoxyribonucleic acid-circ_TTC3 group compared with oxygen-glucose deprivation+oleuropein+plasmid cloning deoxyribonucleic acid group (p<0.05). Oleuropein could reduce oxidative stress and apoptosis, thereby protecting astrocytes from damage induced by oxygen-glucose deprivation by upregulating circ_TTC3 to target microRNA-138-5p.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/oleuropein-inhibits-astrocyte-damage-induced-by-oxygenglucose-deprivation.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoliquiritin Inhibits Laryngeal Squamous Cell Carcinoma Cell Growth and Migration via Downregulating circ_0023028","authors":"Xuejuan Li, Weihua Lou, Fei Ma, Xiaoyi Cha, Changan Pang, Qinghua Ma, Fei Ma","doi":"10.36468/pharmaceutical-sciences.1279","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1279","url":null,"abstract":"Isoliquiritin has anti-tumor effects in a variety of cancers. circ_0023028 has been confirmed to enhance laryngeal squamous cell carcinoma progression. However, whether isoliquiritin affects laryngeal squamous cell carcinoma progression by regulating circ_0023028 is unknown. TU177 cells were divided into 8 groups; control group, isoliquiritin 25 μmol/l group, isoliquiritin 50 μmol/l group, isoliquiritin 100 μmol/l group, si-NC group, si-circ_0023028 group, isoliquiritin+plasmid cloning deoxyribonucleic acid group and isoliquiritin+plasmid cloning deoxyribonucleic acid-circ_0023028 group. Cell functions were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay, colony formation assay, flow cytometry, transwell assay and wound healing assay. circ_0023028 expression was examined by quantitative real-time polymerase chain reaction, and cleaved caspase-3 level was evaluated using Western blot. circ_0023028 expression was higher in laryngeal squamous cell carcinoma tissue. Isoliquiritin increased cell inhibition rate, apoptosis rate and cleaved caspase-3 level, while reduced scratch healing rate, colony cell number, migrated cell number, and circ_0023028 expression in TU177 cells. Silencing of circ_0023028 enhanced cell inhibition rate, apoptosis rate and cleaved caspase-3 level, while suppressed scratch healing rate, colony cell number and migrated cell number in TU177 cells. Besides, overexpression of circ_0023028 reversed the effect of isoliquiritin on laryngeal squamous cell carcinoma cell proliferation, apoptosis and migration. Isoliquiritin inhibited laryngeal squamous cell carcinoma cell growth and migration via reducing circ_0023028 expression.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ijpsonline.com/articles/isoliquiritin-inhibits-laryngeal-squamous-cell-carcinoma-cell-growth-and-migration-emviaem-downregulating-circ0023028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Pharmacist Counselling on Inhaler Usage Technique on Therapeutic Outcomes in Asthma and Chronic Obstructive Pulmonary Disease Patients","authors":"B. Manisha, B. Aishwarya, B. Shekar, P. Mahesh, R. Adepu, A. R Sai Pawan","doi":"10.36468/pharmaceutical-sciences.1167","DOIUrl":"https://doi.org/10.36468/pharmaceutical-sciences.1167","url":null,"abstract":"An open label prospective interventional study was conducted on asthma and chronic obstructive pulmonary disease patients to assess the influence of pharmacist provided education on inhaler usage technique and its impact on therapeutic outcomes in patients with Asthma and chronic obstructive pulmonary disease. The study was approved by the institutional ethics committee and conducted in the pulmonology department of Kamineni Institute of Medical Sciences, Narketpalli, Telangana. Written informed consent was obtained from the recruited study patients. A suitably designed data collection form was designed to capture disease and lab details i.e. forced expiratory volume in the 1st second. A newly constructed and validated knowledge, attitude and practices questionnaire was administered to assess the knowledge about the disease and its management. An inhaler checklist was applied to every patient to assess the inhaler usage technique and provided suitable guidance to use the inhaler effectively and assessed the impact of education on the patient's therapeutic outcomes. Among the 20 patients, 11 (55 %) were females and 9 (45 %) were males. The mean age of the study patients was 54±3 y. A significant improvement in knowledge, attitude and practices scores was observed in the post-counselling stage (p<0.001) and inhaler usage technique (p<0.001) among the patient after providing the counselling. Correct education about disease and inhaler usage techniques had shown an improvement in patient ̓s therapeutic outcomes.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135360666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}