Homeopathy最新文献

{"title":"Multidimensional Outcome Parameters in a Cress Seedling-CuCl2 Crystallization Assay to Corroborate Specific Effects of Stannum metallicum 30x Compared to Lactose 30x.","authors":"Paul Doesburg, Jens-Otto Andersen, Claudia Scherr, Maria Olga Kokornaczyk, Stephan Baumgartner","doi":"10.1055/s-0044-1785517","DOIUrl":"https://doi.org/10.1055/s-0044-1785517","url":null,"abstract":"<p><strong>Background: </strong> Previously we developed a test system which yielded highly significant evidence for specific effects of a <i>Stannum metallicum</i> 30x preparation in a multi-center replication trial. This test system is based on cress seed germination in homeopathic or control samples, CuCl₂ crystallization of the cress extract, and subsequent digital textural image analysis of the resulting crystallization patterns.</p><p><strong>Objectives: </strong> The current study aimed to investigate whether three novel outcome parameters could further corroborate and possibly characterize the specific effects of <i>Stannum metallicum</i> 30x.</p><p><strong>Methods: </strong> To this end, (1) cress seedling length, (2) a second texture analysis parameter, entropy and (3) the local connected fractal dimension (LCFD) of crystallization patterns as a measure of complexity were considered. The stability of the experimental setup was monitored throughout the entire investigation with systematic negative control (SNC) experiments.</p><p><strong>Results: </strong> Cress length and entropy revealed a time-modulated potency treatment effect, in the absence of a significant main treatment effect. This indicated that the effect of the potency treatment varied significantly across the different experimental days. LCFD yielded a highly significant potency treatment effect. In addition, a significant interaction of treatment with experimental day seems to indicate a modulation of this effect. No significant effects were observed in any of the evaluations of the SNC experiments, indicative of a stable experimental setup and a reliable and specific treatment effect. Neither significant nor strong correlations were found between the four parameters, indicating that they reflect different effects of <i>Stannum metallicum</i> 30x on the organism treated.</p><p><strong>Conclusion: </strong> This quadruple characterization of the biological effects of <i>Stannum metallicum</i> 30x provides an unprecedented opportunity for basic homeopathy research into, among others, the presumed specificity of homeopathic preparations.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Patient Population at Homeopathic Outpatient Clinics across India: A Clinical Data Collection Study.","authors":"Harleen Kaur, Surbhi Jain, Daisy Katarmal, Jyoti Sachdeva, Hima Bindu Ponnam, K C Muraleedharan, Suraia Parveen, Sonia Raizada, Liyi Karso, Renu Bala, Amit Srivastava, Vaishali Shinde, Sunil Ramteke, Gurudev Choubey, Chittaranjan Kundu, Vinitha E Ramanan, Tejaswini Patole, Ranjit Sonny, Baidurjya Bhattacharjee, Ravi Kumar Sardarla, Ramesh S Bawaskar, G R C Reddy, Kumar Keshav Avinash, Santosh Tamang, Akhyaya Kumar Prusty, Madhumita Sadhukhan, Artemis Maglara, Anastasia Garoufali, Michael Stassinopoulos, Theodoros Lilas, Lefteris Tapakis, Anil Khurana, Raj K Manchanda","doi":"10.1055/s-0044-1782221","DOIUrl":"https://doi.org/10.1055/s-0044-1782221","url":null,"abstract":"<p><strong>Background: </strong> Even though several initiatives have been undertaken in different locations worldwide to collect clinical data in homeopathy, it is important to further investigate these aspects in the context of health care in India.</p><p><strong>Objective: </strong> The study aimed to gather and analyze patients' clinical data and to derive insights into homeopathic treatment using an internet-based software program for data storage, retrieval and repertorization.</p><p><strong>Methods: </strong> A multi-center observational study was conducted across 14 homeopathy outpatient clinics in India that are affiliated with the Central Council for Research in Homoeopathy (CCRH). Patient symptoms and demographic details were documented anonymously, and prescriptions were guided by repertorial suggestions from the Vithoulkas Compass software. During follow-up visits, treatment outcome was also recorded using an online assessment form. A retrospective analysis of data on patients' demographics, follow-up visits, morbidity (International Classification of Diseases 11th Revision), rubrics used, prescribed medicines and the level of improvement was achieved using Microsoft Excel-generated pivot tables.</p><p><strong>Results: </strong> Throughout the study duration of one year a total of 2,811 patients attended the 14 outpatient clinics, of whom 2,468 were new patients with a total of 2,172 initial homeopathic prescription entries. Across the study, there were 3,491 prescriptions and 1,628 follow-up consultations for 868 follow-up patients, all of which data were thoroughly analyzed. The highest frequency of patients was in the 20-49 age group, and a higher proportion of the patients overall was female. Musculoskeletal, dermatological and respiratory complaints were the most frequently reported. The rubrics \"Desire for sweets\" and \"Desire for spices\" emerged as the most commonly used in the repertorizations. Further, <i>Sulphur</i> stood out as the most commonly prescribed medicine overall. With homeopathic treatment, some degree of clinical improvement was reported in 86% of the follow-up cases.</p><p><strong>Conclusion: </strong> Homeopathy is prescribed in CCRH outpatient clinics for a wide range of ailments in people across India, with at least some clinical improvement noted in a high proportion of those patients. The large-scale systematic data collection in these clinics has provided clear insights into the use and clinical value of homeopathy in India, with the potential to build a substantive nationwide data inventory over time.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homeopathy as a Means to Improve Quality of Life.","authors":"Fernanda Maria Simões da Costa Fujino, Ana Paula Ribeiro, Jane de Eston Armond, Patrícia Colombo de Souza","doi":"10.1055/s-0044-1785521","DOIUrl":"https://doi.org/10.1055/s-0044-1785521","url":null,"abstract":"<p><p>Quality of life is a fundamental aspect of good health and differs from person to person, highlighting the importance of individualisation, which is one of the principles of homeopathic doctrine. Homeopathy aligns with the principles of the Brazilian public health system, reflecting the latter's ethos of universality, accessibility, care coordination, and comprehensiveness, offering a whole-person approach to health care. Homeopathy's individualised approach and expanded view of the health-disease process, with emphasis on healthy lifestyle guidance, contributes to the promotion of individual good health and quality of life. These attributes of homeopathy make it a valuable therapeutic option, with relevance to the health service of Brazil as well as to that of other countries across the world.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol Standardization of Homeopathic Medicines' Preparation and Dosing for Experimental Model Systems In Vitro.","authors":"Nilanjana Basu, Simran Tandon","doi":"10.1055/s-0044-1786513","DOIUrl":"https://doi.org/10.1055/s-0044-1786513","url":null,"abstract":"","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Why Homeopathic Medicines are Used for Menopause: Searching for Insights into Neuroendocrine Features.","authors":"Emma Macías-Cortés","doi":"10.1055/s-0043-1769734","DOIUrl":"10.1055/s-0043-1769734","url":null,"abstract":"<p><strong>Background: </strong>Menopause is a physiological event that marks the end of a woman's reproductive stage in life. Vasomotor symptoms and changes in mood are among its most important effects. Homeopathy has been used for many years in treating menopausal complaints, though clinical and pre-clinical research in this field is limited. Homeopathy often bases its prescription on neuropsychiatric symptoms, but it is unknown if homeopathic medicines (HMs) exert a neuroendocrine effect that causes an improvement in vasomotor symptoms and mood during menopause.</p><p><strong>Objectives: </strong>The study's objectives were to address the pathophysiological changes of menopause that could help in the understanding of the possible effect of HMs at a neuroendocrine level, to review the current evidence for two of the most frequently prescribed HMs for menopause (<i>Lachesis mutus</i> and <i>Sepia officinalis</i>), and to discuss the future directions of research in this field.</p><p><strong>Methods: </strong>An extensive literature search for the pathophysiologic events of menopause and depression, as well as for the current evidence for HMs in menopause and depression, was performed.</p><p><strong>Results: </strong>Neuroendocrine changes are involved in the pathophysiology of vasomotor symptoms and changes in mood during menopause. Gonadal hormones modulate neurotransmitter systems. Both play a role in mood disorders and temperature regulation. It has been demonstrated that <i>Gelsemium sempervirens</i>, <i>Ignatia amara</i> and <i>Chamomilla matricaria</i> exert anxiolytic effects in rodent models. <i>Lachesis mutus</i> and <i>Sepia officinalis</i> are frequently prescribed for important neuropsychiatric and vasomotor symptoms. Dopamine, a neurotransmitter involved in mood, is among the constituents of the ink of the common cuttlefish, <i>Sepia officinalis</i>.</p><p><strong>Conclusion: </strong>Based on all the pathophysiologic events of menopause and the improvement in menopausal complaints that certain HMs show in daily practice, these medicines might have a direct or indirect neuroendocrine effect in the body, possibly triggered via an as-yet unidentified biological mechanism. Many unanswered questions in this field require further pre-clinical and clinical research.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"54-66"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9749623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strange, Rare and Peculiar Symptoms of COVID-19 Cases Managed by Practitioners of the Homeopathy Help Network.","authors":"Parker Pracjek, Alastair C Gray, Rachael Doherty, Denise Straiges","doi":"10.1055/s-0043-1771374","DOIUrl":"10.1055/s-0043-1771374","url":null,"abstract":"<p><strong>Background: </strong>More than 670 million people have been infected by COVID-19. This case series reports 8 of 55 cases in a broader study of COVID-positive clients who sought homeopathic care for symptoms. Existing studies of homeopathy and COVID-19 have sometimes failed to employ the underpinning theoretical framework of homeopathy-the <i>genus epidemicus</i>. Special focus has been placed on standout symptoms not often reported in conventional medical outlets, known among homeopaths as \"strange, rare and peculiar\" (SRP) symptoms. The Homeopathy Help Network (HHN) team of practitioners noted SRP symptoms across dozens of cases and studied how they shifted collectively as different variants of the virus emerged.</p><p><strong>Methods: </strong>COVID-positive individuals self-selected for individualized care for their symptoms using homeopathy. They received tele-health consultations and individualized homeopathy interventions in an out-patient homeopathy clinical setting. Clients were seen by individual professional homeopathy practitioners and students under supervision working at the HHN in the United States. Cases for the series were hand-picked with the aim of being an average representation of the more than 4,000 COVID-positive cases seen by members of the HHN. Cases in the full compendium are grouped according to a predominant case feature: Multiple remedies, Posology, Time ill, Single remedy resolution, Hospitalization and, in this case series, SRP symptoms.</p><p><strong>Results: </strong>SRP symptoms included: continually on the verge of unconsciousness; dark green stools; very low pulse alternating with tachycardia; sensation of strong or burning chemical smells; sensation of inhaling water through the nose; recurring electric shock sensations in head or extremities; yellow-green stools.</p><p><strong>Conclusion: </strong>Collective SRP symptoms from the pandemic provided the opportunity to study the hallmark features of COVID-19 in depth. The importance of these symptoms highlights the applicability of Hahnemannian principles and good case-taking practices.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"112-125"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11027477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71423331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomised Controlled Trial on Prophylaxis of Mastitis-Metritis-Agalactia Syndrome in Swine using Caulophyllum Logoplex and Lachesis Logoplex.","authors":"Kathrin Büttner, Janna Oelkers, Onno Burfeind, Joachim Krieter, Irena Czycholl","doi":"10.1055/s-0043-1770725","DOIUrl":"10.1055/s-0043-1770725","url":null,"abstract":"<p><strong>Background: </strong>Mastitis-metritis-agalactia (MMA) syndrome occurs in the first days post-partum and causes piglet losses mainly due to malnutrition. One possibility for prophylaxis of MMA is via homeopathy. In this veterinary study, the effectiveness of a prophylactic administration of homeopathic remedies for the prevention of the occurrence of MMA in swine was evaluated.</p><p><strong>Methods: </strong>In a randomised and blinded study, 60 sows were examined. Sows were randomly distributed in two groups: the experimental group (CL/LL) received a prophylactic administration of the complex homeopathic remedies <i>Caulophyllum Logoplex</i> and <i>Lachesis Logoplex</i>, and the placebo group was administered a sodium chloride (NaCl) solution in the same injection scheme as the experimental group. Clinical signs of MMA, behavioural changes, as well as production parameters, were recorded beginning with the day of farrowing until 5 days post-partum.</p><p><strong>Results: </strong>The treatment group showed no significant effect on the occurrence of MMA in sows (CL/LL: 56.67% MMA positive sows; NaCl: 53.53% MMA positive sows). Treatment group had also no significant effect on health parameters (vaginal discharge, raised rectal temperature, shortage of milk) or behavioural parameters (impaired feeding behaviour and impaired general condition). For the production parameter average weight gain, statistically significant effects in the treatment group were detected.</p><p><strong>Conclusions: </strong>Prophylaxis with the homeopathic remedies <i>Caulophyllum Logoplex</i> and <i>Lachesis Logoplex</i> showed neither an improvement in MMA prevention nor an improvement in health parameters or behavioural traits in the present herd of sows.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"80-85"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10128067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Double-Blind, Randomized, Placebo-Controlled Clinical Trial to Evaluate the Efficacy of Individualized Homeopathic Medicines in Pre-diabetes.","authors":"Nilanjana Guha, Shubhamoy Ghosh, Sanjukta Mandal, Aakash Deep Das, Chithra Palanisamy, Shukdeb Maiti, Priyanka Ghosh, Navin Kumar Singh, Munmun Koley, Subhranil Saha","doi":"10.1055/s-0043-1769506","DOIUrl":"10.1055/s-0043-1769506","url":null,"abstract":"<p><strong>Background: </strong>Pre-diabetes (PD) contributes importantly to the disease burden worldwide and is a precursor to stroke, cardiovascular diseases, as well as type-2 diabetes mellitus.</p><p><strong>Objective: </strong>In this project, the efficacy of individualized homeopathic medicines (IHMs) was explored against placebos in the treatment of PD.</p><p><strong>Methods: </strong>A 6-month, double-blind, randomized, placebo-controlled trial was conducted at the outpatient departments of a homeopathic medical college and hospital in India. Sixty participants with PD were randomized to receive either IHMs (<i>n</i> = 30) or identical-looking placebos (<i>n</i> = 30). Concomitant care measures were advised to both groups of participants in terms of dietary advice, yoga, meditation and exercise. The primary outcome measures were fasting blood sugar (FBS) and the oral glucose tolerance test (OGTT); the secondary outcome was the Diabetes Symptom Checklist-Revised (DSC-R) score. All the outcomes were measured at baseline and after 3 and 6 months of treatment. Inter-group differences and effect sizes (Cohen's <i>d</i>) were calculated using two-way repeated measures analysis of variance models after adjusting baseline differences using analysis of co-variance on the intention-to-treat data.</p><p><strong>Results: </strong>Between-group differences for FBS were statistically significant, favoring IHMs against placebos (<i>F</i> _1,58 = 7.798, <i>p</i> = 0.007), but not for OGTT (<i>F</i> _1,58 = 1.691, <i>p</i> = 0.199). The secondary outcome, DSC-R total score, favoring IHMs significantly compared with placebos (<i>F</i> _1,58 = 15.752, <i>p</i> < 0.001). <i>Calcarea Carbonicum</i>, <i>Thuja occidentalis</i> and <i>Sulphur</i> were the most frequently prescribed medicines. No harm or serious adverse events were recorded from either of the participant groups.</p><p><strong>Conclusion: </strong>IHMs produced significantly better results than placebos in FBS and in DSC-R scores but not in OGTT. Independent replications with larger sample sizes are warranted to substantiate the findings.</p><p><strong>Trial registration: </strong>CTRI/2019/10/021711.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"67-79"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10063405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and Sub-acute Oral Toxicity Assessment of Ferrum phosphoricum in Rats.","authors":"Mahima Sharma, Sangita Behera, Raj Kumar Regar, Deepika Tripathi, Satvinder Singh, Narasimha G V Kumar, Pankaj Gupta","doi":"10.1055/s-0043-1770338","DOIUrl":"10.1055/s-0043-1770338","url":null,"abstract":"<p><strong>Background: </strong><i>Ferrum phosphoricum</i> (FP) has been used by traditional medicine practitioners for various ailments since ancient times. However, scientific evidence on the safety of FP is still unavailable. Thus, the current study aimed to investigate the acute and sub-acute oral toxicity of homeopathic FP in experimental rats.</p><p><strong>Methods: </strong>In an acute toxicity investigation, a single dose of 2,000 µL/kg of FP 6c, 30c and 200c was administered to female Wistar rats, which were monitored for up to 14 days according to the Organization for Economic Cooperation and Development (OECD) guideline 423. For a sub-acute toxicity study, FP 6c, 30c and 200c (200 µL/kg) were administered to male and female rats for 28 days as per the OECD guideline 407. All the animals were observed for mortality, clinical signs and body weight during the study. At the end of the experiment, hematological, biochemical and histopathological assessments were performed.</p><p><strong>Results: </strong>During the acute toxicity study, no mortality was observed in rats administered with FP, and thus the median lethal dose (LD₅₀) was identified as >2,000 µL/kg. In the sub-acute study, no mortality or adverse clinical signs were noticed with FP treatment. Moreover, weekly body weight gain was normal. Hematological and biochemical investigations revealed no abnormalities. Furthermore, histological analysis of FP-treated rats' vital organs revealed no pathological changes.</p><p><strong>Conclusion: </strong>Overall, our findings imply that FP 6c, 30c and 200c potencies are safe and do not cause toxicity when given orally to Wistar albino rats for an extended period at a dose of 200 µL/kg.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"86-97"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10096371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homeopathic Formulations of Syzygium jambolanum Alleviate Glycation-Mediated Structural and Functional Modifications of Albumin: Evaluation through Multi-Spectroscopic and Microscopic Approaches.","authors":"Nilima S Bangar, Selvan Ravindran, Shamim A Shaikh, Nilesh Shah, Rashmi S Tupe","doi":"10.1055/s-0043-1771024","DOIUrl":"10.1055/s-0043-1771024","url":null,"abstract":"<p><strong>Background: </strong>The growing interest in identifying the mode of action of traditional medicines has strengthened its research. <i>Syzygium jambolanum</i> (<i>Syzyg</i>) is commonly prescribed in homeopathy and is a rich source of phytochemicals.</p><p><strong>Objective: </strong>The present study aims to shed light on the anti-glycation molecular mechanism of <i>Syzyg</i> mother tincture (MT), 30c, and 200c on glycated human serum albumin (HSA) by multi-spectroscopic and microscopic approaches.</p><p><strong>Methods: </strong>The phytochemicals and antioxidant potential of the <i>Syzyg</i> formulations were estimated by the high-performance liquid chromatography and spectroscopic technique, respectively. Glycation was initiated by incubating HSA with methylglyoxal, three <i>Syzyg</i> formulations, and the known inhibitor aminoguanidine in separate tubes at 37°C for 48 hours. The formation of glycation adducts was assessed by spectrofluorometer and affinity chromatography. The structural modifications were analyzed through circular dichroism, Fourier transform infrared spectroscopy, turbidity, 8-anilinonapthalene-1-sulfonic acid fluorescence, and nuclear magnetic resonance. Further, the formation of the aggregates was examined by thioflavin T, native-polyacrylamide gel electrophoresis, and transmission electron microscopy. Additionally, the functional modifications of glycated HSA were determined by esterase-like activity and antioxidant capacity. The binding analysis of <i>Syzyg</i> formulations with glycated HSA was evaluated by surface plasmon resonance (SPR).</p><p><strong>Results: </strong><i>Syzyg</i> formulations MT, 30c, and 200c contained gallic acid and ellagic acid as major phytochemicals, with concentrations of 16.02, 0.86, and 0.52 µg/mL, and 227.35, 1.35, and 0.84 µg/mL, respectively. Additionally, all three formulations had remarkable radical scavenging ability and could significantly inhibit glycation compared with aminoguanidine. Further, <i>Syzyg</i> formulations inhibited albumin's structural and functional modifications. SPR data showed that <i>Syzyg</i> formulations bind to glycated HSA with an equilibrium dissociation constant of 1.10 nM.</p><p><strong>Conclusion: </strong><i>Syzyg</i> formulations inhibited the glycation process while maintaining the structural and functional integrity of HSA.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"98-111"},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49677033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}