HomeopathyPub Date : 2024-08-01Epub Date: 2023-10-31DOI: 10.1055/s-0043-1775993
Fabrice Sébastien Berna, Jean-Lionel Bagot
{"title":"Probabilistic Reasoning in Homeopathy Practice and its Implications for Clinical Research.","authors":"Fabrice Sébastien Berna, Jean-Lionel Bagot","doi":"10.1055/s-0043-1775993","DOIUrl":"10.1055/s-0043-1775993","url":null,"abstract":"<p><p>The principle of similitude put forward by Hahnemann has challenging practical consequences in the selection of the right homeopathic medicine for a patient. According to this principle, only the medicines that best fit the totality of the symptoms of a given patient are supposed to really cure: this greatly depends on the homeopath's clinical analysis. In addition, a patient's illness may be more or less curable, depending on the characteristics of the disease. In their daily practice, homeopaths typically apply Bayesian reasoning to deal with uncertainty associated with both medicine and disease. We suggest that clinical research on homeopathy would gain by integrating this kind of prior estimation of (1) the probability of a given medicine being effective for that particular patient and (2) the probability of the patient's disease (or symptoms) being curable. We therefore suggest that future trials of N-of-1 design may gain (1) by testing a small number of \"best candidate\" medicines (instead of one) for a given patient facing a given disease, and (2) by including careful prior estimations of the probabilities that (a) each selected medicine will be efficient for that patient and (b) the patient's disease will be reversible with the medicine.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"167-175"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71423330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-08-01Epub Date: 2023-10-30DOI: 10.1055/s-0043-1775815
Jozélio Freire de Carvalho, Maria Fernanda Leal Dos Santos Ribeiro
{"title":"Successful Treatment of a Fibromyalgia Patient Using a Homeopathic Preparation of Cannabis sativa.","authors":"Jozélio Freire de Carvalho, Maria Fernanda Leal Dos Santos Ribeiro","doi":"10.1055/s-0043-1775815","DOIUrl":"10.1055/s-0043-1775815","url":null,"abstract":"<p><strong>Background: </strong>Homeopathy has been used in observational and controlled studies to treat patients with fibromyalgia (FM), but none has previously used the remedy <i>Cannabis sativa</i>.</p><p><strong>Case history: </strong>A 51-year-old female patient presenting with diffuse pain and sleep disorder was diagnosed with FM using the relevant American College of Rheumatology criteria. She reported having 18 tender points, a pain score (visual analog scale, VAS) of 9.0, and a well-being VAS of 5.0. She was prescribed <i>Cannabis sativa</i> 6 cH, five drops sublingually thrice a day.</p><p><strong>Results: </strong>After 2 months, she returned asymptomatic, with 0 tender points, pain VAS of 0, and well-being VAS of 9.0. The Modified Naranjo Criteria for Homeopathy score was equal to +9, suggesting the clinical outcome was causally attributable to the medicine prescribed.</p><p><strong>Conclusion: </strong>This case study reveals the positive role of homeopathic treatment in FM. Studies using a randomized controlled design, including pragmatic trials to determine treatment effectiveness in real-world clinical practice, are indicated in this field.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"186-189"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71412058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-08-01Epub Date: 2023-09-27DOI: 10.1055/s-0043-1770360
Giuliano Pereira de Barros, Denise Pereira Leme, Laura Livia Arias Avilés, Patrizia Ana Bricarello
{"title":"Homeopathic Sulphur Ointment as Adjuvant in the Treatment of Sheep with Myiasis by Cochliomyia hominivorax (Diptera: Calliphoridae): A Case Series.","authors":"Giuliano Pereira de Barros, Denise Pereira Leme, Laura Livia Arias Avilés, Patrizia Ana Bricarello","doi":"10.1055/s-0043-1770360","DOIUrl":"10.1055/s-0043-1770360","url":null,"abstract":"<p><strong>Background: </strong>Myiasis by <i>Cochliomyia hominivorax</i> (Diptera: Calliphoridae) is a serious problem in animal health. Homeopathic <i>Sulphur</i> presents similarity with the clinical presentation verified in these cases and has already shown <i>in vitro</i> efficacy on the blowfly that causes this pathology. This article presents the report of a series of clinical cases of myiasis by <i>C. hominivorax</i> in sheep that underwent adjuvant treatment with homeopathic <i>Sulphur</i> and presents a theoretical-scientific discussion of biological events related to the clinical treatment of the condition.</p><p><strong>Patients: </strong>Seven naturally occurring myiases affecting sheep from an experimental farm were treated by an ointment impregnated with the homeopathic medicine <i>Sulph</i> 12cH, used as adjuvant after the manual removal of accessible larvae from the wound during wound cleaning. The animals were treated daily and evaluated clinically until complete healing of the lesions. Clinical information on the evolution of the inflammatory and scarring process was collected. Blood counts and bacteriological examinations were performed before and after topical homeopathic treatment.</p><p><strong>Results: </strong>The homeopathic ointment <i>Sulph</i> 12cH was able to inhibit the development of parasitic <i>C. hominivorax</i> larvae and promote tissue repair and healing in naturally occurring myiasis in the sheep of this study.</p><p><strong>Conclusion: </strong>The homeopathic medicine <i>Sulphur</i>, formulated as an ointment, may be a useful new and adjuvant therapeutic option for treating myiasis in animals.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"190-204"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41126347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-08-01Epub Date: 2023-11-01DOI: 10.1055/s-0043-1771023
Ashish Kumar Dixit, Danish Javed, Amit Srivastava, Renu Bala, Nibha Giri
{"title":"Homeopathic Medicines in the Management of Dermatophytosis (Tinea Infections): A Clinico-epidemiological Study with Pre-post Comparison Design.","authors":"Ashish Kumar Dixit, Danish Javed, Amit Srivastava, Renu Bala, Nibha Giri","doi":"10.1055/s-0043-1771023","DOIUrl":"10.1055/s-0043-1771023","url":null,"abstract":"<p><strong>Background: </strong>Dermatophytosis is a common fungal infection of the skin and nails. Insufficient data exist regarding the clinico-epidemiological profile of dermatophytosis and the usefulness of individualized homeopathic medicines (IHMs) for patients visiting a homeopathy outpatient department (OPD).</p><p><strong>Objectives: </strong>This article undertakes a clinico-epidemiological profiling of dermatophytosis and the usefulness of IHMs in its management.</p><p><strong>Methods: </strong>This open-label, pre-post, comparative observational study was conducted in a homeopathy OPD from November 2018 to February 2020. IHMs were prescribed based on symptom totality and repertorization. A numeric rating scale (NRS) and the Dermatology Life Quality Index (DLQI) patient questionnaires were used, and results were analyzed using SPSS-IBM version 20.</p><p><strong>Results: </strong>Data from a total of 103 patients, mean age 29.65 ± 15.40 years, were analyzed. <i>Tinea cruris</i> was the most common infection (29.1%), followed by <i>tinea corporis</i> (13.6%). After 3 months of treatment, significant reductions in NRS and DLQI scores were observed (8.51 ± 1.24 to 0.59 ± 0.83, <i>p</i> < 0.001, and 16.28 ± 5.30 to 1.44 ± 1.56, <i>p</i> < 0.001, respectively), with <i>Sepia</i> (15.5%), <i>Sulphur</i> (14.6%), <i>Calcarea carbonica</i> (11.7%), <i>Natrum muriaticum</i> (9.7%) and <i>Bacillinum</i> (8.7%) being the most frequently prescribed medicines. There was no significant correlation between occupation, sex, home location or marital status and the clinical types of dermatophytosis. No adverse events were reported.</p><p><strong>Conclusion: </strong><i>T. cruris</i> and <i>T. corporis</i> were prevalent dermatophytic infections. The decrease in NRS and DLQI scores associated with homeopathy indicates its usefulness as an integrative treatment option for dermatophytosis. Further research in larger and more diverse population samples is needed.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"149-157"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71423329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-08-01Epub Date: 2023-09-25DOI: 10.1055/s-0043-1771025
Soumya Bhattacharyya, Chandrima Chatterjee, Subhranil Saha, Satyajit Naskar, Pulakendu Bhattacharya, Sk Monsur Alam, Sumana Sengupta, Sabir Ahamed, Abdur R Shaikh, Munmun Koley, Priyanka Ghosh, Shyamal K Mukherjee
{"title":"Individualized Homeopathic Medicines in the Treatment of Knee Osteoarthritis: Double-Blind, Randomized, Placebo-Controlled Feasibility Trial.","authors":"Soumya Bhattacharyya, Chandrima Chatterjee, Subhranil Saha, Satyajit Naskar, Pulakendu Bhattacharya, Sk Monsur Alam, Sumana Sengupta, Sabir Ahamed, Abdur R Shaikh, Munmun Koley, Priyanka Ghosh, Shyamal K Mukherjee","doi":"10.1055/s-0043-1771025","DOIUrl":"10.1055/s-0043-1771025","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed at examining the feasibility issues of comparing individualized homeopathic medicines (IHMs) with identical-looking placebos for treating knee osteoarthritis (OA).</p><p><strong>Methods: </strong>Forty eligible patients participated in this double-blind, randomized (1:1), placebo-controlled feasibility trial in the outpatient clinics of a homeopathic hospital in West Bengal, India. Either IHMs or identical-looking placebos were administered, along with mutually agreed-upon concomitant care guidelines. The Knee Injury and Osteoarthritis Outcome Score (KOOS) was the primary outcome measure, along with derived Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores from KOOS. The EQ-5D-5L questionnaire and Visual Analog Scale (VAS) were the secondary outcomes. All were measured at baseline and after 2 months. Group differences and effect sizes (Cohen's <i>d</i>) were estimated using an intention-to-treat approach. <i>p</i>-Values less than 0.05 (two-tailed) were considered statistically significant.</p><p><strong>Results: </strong>Enrolment/screening and trial retention rates were 43% and 85% respectively. Recruitment was difficult owing to the coronavirus disease 2019 (COVID-19) lockdown. Group differences were statistically significant, favoring IHMs against placebos in all the KOOS sub-scales: symptoms (<i>p</i> < 0.001), pain (<i>p</i> = 0.002), activities of daily living (<i>p</i> < 0.001), sports or recreation (<i>p</i> = 0.016), and quality of life (<i>p</i> = 0.002). Derived WOMAC scores from KOOS favored IHMs against placebos: stiffness (<i>p</i> < 0.001) and pain (<i>p</i> < 0.001). The EQ-5D-5L questionnaire score (<i>p</i> < 0.001) and EQ-5D-5L VAS scores (<i>p</i> < 0.001) also yielded significant results, favoring IHMs over placebos. All the effect sizes ranged from moderate to large. <i>Sulphur</i> was the most frequently prescribed homeopathic medication. Neither group reported any harm or serious adverse events.</p><p><strong>Conclusion: </strong>Although recruitment was sub-optimal due to prevailing COVID-19 conditions during the trial, the action of IHMs was found to be superior to that of placebos in the treatment of knee OA. Larger and more definitive studies, with independent replications, are warranted in order to substantiate the findings.</p><p><strong>Trial registration: </strong>CTRI/2021/02/031453.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":"158-166"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41121010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-08-01Epub Date: 2024-07-26DOI: 10.1055/s-0044-1787816
Robert T Mathie
{"title":"Principles in Practice.","authors":"Robert T Mathie","doi":"10.1055/s-0044-1787816","DOIUrl":"10.1055/s-0044-1787816","url":null,"abstract":"","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":"113 3","pages":"131"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-07-10DOI: 10.1055/s-0044-1786511
Edgard Costa de Vilhena, Nicolle R Telles Coutracos, Ednar N Coimbra, Karen Berenice Denez, Adriana Passos Oliveira, Carla Holandino, Vânia Emerich Bucco de Campos, Giovanni Bravin Peres, Leoni Villano Bonamin
{"title":"Prevalence of COVID-19 Complications during a Program of Homeopathic Camphora Officinalis Distribution to City Populations of Santa Catarina, Brazil: An Ecological Study.","authors":"Edgard Costa de Vilhena, Nicolle R Telles Coutracos, Ednar N Coimbra, Karen Berenice Denez, Adriana Passos Oliveira, Carla Holandino, Vânia Emerich Bucco de Campos, Giovanni Bravin Peres, Leoni Villano Bonamin","doi":"10.1055/s-0044-1786511","DOIUrl":"https://doi.org/10.1055/s-0044-1786511","url":null,"abstract":"<p><strong>Background: </strong> Coronavirus disease 2019 (COVID-19), a disease caused by the SARS-CoV-2 coronavirus, was declared a pandemic in March 2020, posing significant challenges globally. Homeopathy has historical relevance in epidemic management. In response, the government of the state of Santa Catarina, Brazil, distributed <i>Camphora</i> 1M as a potential prophylactic intervention for COVID-19.</p><p><strong>Objective: </strong> This study aimed to investigate the possible effects of <i>Camphora</i> 1M as an adjunctive prophylactic measure in managing COVID-19, focusing on mortality and hospitalization rates, during the period April 28 to July 31, 2020, within designated COVID-19 in-patient units in Santa Catarina.</p><p><strong>Methods: </strong> An ecological study design was applied to this epidemiological research. Five case municipalities (Itajaí, Atalanta, Entre Rios, Rio do Campo, Trombudo Central) were compared with five control municipalities (São José, Galvão, Pedras Grandes, Grão-Pará, Ascurra).</p><p><strong>Results: </strong> No statistically significant differences were observed in predictor variables between municipalities that received <i>Camphora</i> 1M and the respective controls. Similarly, no statistically significant differences were observed in outcomes: deaths (<i>p</i> = 0.879), hospitalized cases (<i>p</i> = 0.537), daily ward admissions (<i>p</i> = 0.730) and ICU admissions (<i>p</i> = 0.072).</p><p><strong>Conclusion: </strong> For the first wave of the pandemic in the state of Santa Catarina, Brazil, city-wide distribution of <i>Camphora</i> 1M was not associated with reduced numbers, severity or mortality among the population hospitalized in designated public hospitals for COVID-19.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of the Likelihood Ratio of Different Symptoms for Six Homeopathic Medicines: Prognostic Factor Research.","authors":"Aditi Mohanta, Nipa Sardar, Koli Chakraborty, Malabika Sarkar, Tamoghna Saha, Anuruddha Deb, Md Sakhawat Hossain, Arpita Basu, Saima Samim, Soumya Bhattacharyya, Sangita Saha, Biswajit Basu, Anindya Sen, Mohan Giri, Pintu Debnath, Subhranil Saha, Munmun Koley, Shyamal Kumar Mukherjee, Lex Rutten","doi":"10.1055/s-0044-1787078","DOIUrl":"https://doi.org/10.1055/s-0044-1787078","url":null,"abstract":"<p><strong>Background: </strong> <i>Arsenicum album</i>, <i>Causticum</i>, <i>Nux vomica</i>, <i>Pulsatilla nigricans</i>, <i>Rhus toxicodendron</i> and <i>Sulphur</i> are frequently prescribed homeopathic medicines; however, their symptoms, as mentioned in different homeopathic literature works, have rarely been investigated systematically. Likelihood ratio (LR), based on Bayesian statistics, may reflect a better estimation of the strengths of symptoms than the existing entries in the homeopathic literature.</p><p><strong>Methods: </strong> A prospective, longitudinal, analytical patient outcome study was conducted in the outpatient departments of D. N. De Homeopathic Medical College and Hospital, Kolkata, on 1,954 patients over 21 months. The outcomes were recorded at each follow-up using the Outcome Related to Impact on Daily Living (ORIDL) +4 to -4 scale. The average period of treatment for each participant was 3 months. The LRs of four symptoms for each of the six selected medicines were calculated.</p><p><strong>Results: </strong> One hundred and two different remedies were prescribed. The prevalence, LR + , and LR - , with respective 95% confidence intervals, of different symptoms were reported. The study found that the following symptoms had particularly high LR+ scores: \"intense sympathy for the suffering of others\" (<i>Causticum</i>, LR+ = 12.0); \"dyspepsia from business anxiety\" (<i>Nux vomica</i>, LR+ = 27.4); \"burning pain relieved by heat\" (<i>Arsenicum album</i>, LR+ = 29.6); \"envy\" (<i>Pulsatilla nigricans</i>, LR+ = 13.2); \"desire for milk\" (<i>Rhus toxicodendron</i>, LR+ = 7.5); \"very selfish, no regard for others\" (<i>Sulphur</i>, LR+ = 20.6). The findings corroborated well with the presentation of the symptoms in different homeopathic materia medica and repertories. ORIDL scores of +2 or greater were identified most prominently for <i>Pulsatilla nigricans</i> (<i>n</i> = 138) and <i>Sulphur</i> (<i>n</i> = 119).</p><p><strong>Conclusion: </strong> There was adequate evidence to attribute all the assessed symptoms to the medicines investigated. Further studies with a larger population are warranted to tackle the possible confirmation bias.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HomeopathyPub Date : 2024-07-08DOI: 10.1055/s-0044-1786739
Fernanda Maria Simões da Costa Fujino, Jane de Eston Armond, Ana Paula Ribeiro, Patrícia Colombo de Souza
{"title":"Historical Perspective of Homeopathy in the Brazilian Public Health System.","authors":"Fernanda Maria Simões da Costa Fujino, Jane de Eston Armond, Ana Paula Ribeiro, Patrícia Colombo de Souza","doi":"10.1055/s-0044-1786739","DOIUrl":"https://doi.org/10.1055/s-0044-1786739","url":null,"abstract":"<p><p>Homeopathy was introduced in Brazil with the French doctor Benoît Jules Mure's arrival in 1840 and was officially recognised in 1980 as a medical specialty by Brazilian regulatory authorities. Public health policies played an important role in incorporating homeopathy into the Brazilian Unified Health System (SUS), emphasising homeopathy's coherence with SUS's fundamental principles and with other national health policies. Homeopathy is supported by the guidelines of the National Primary Health Care Policy and the National Policy on Integrative and Complementary Practices, and its offer in the SUS has been recognised since 2006. Challenges persist, however, such as the low prevalence of the use of homeopathy, lack of investment in professional training and under-reporting of homeopathy outpatient appointments. Investments in disseminating information on homeopathic philosophy and raising awareness among managers and health professionals are essential to strengthen its presence in the Brazilian public health system.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141558612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultradiluted Homeopathic Medicines Cause Apoptosis in RPMI-8226 Multiple Myeloma Cells in vitro: a Pilot Study.","authors":"Buket Altinok Gunes, Murat Kilic, Tulin Ozkan, Nurbanu Gonulkirmaz, Nurcihan Guven, Asuman Sunguroglu","doi":"10.1055/s-0044-1786035","DOIUrl":"https://doi.org/10.1055/s-0044-1786035","url":null,"abstract":"<p><strong>Background: </strong> Multiple myeloma (MM) is the second most common type of cancer among hematological malignancies and is difficult to treat. Although controversial in nature, homeopathy's effects have been tested on a wide range of cancer cell types <i>in vitro</i>, as well as clinically. However, homeopathic medicines have yet to be tested in MM cells. In this preliminary study, we investigated the effects of <i>Arsenicum album</i>, <i>Hecla lava</i>, <i>Carcinosinum</i> and <i>Carboneum sulphuratum</i> 200C on a human MM cell line.</p><p><strong>Methods: </strong> The RPMI-8226 MM cell line was cultured <i>in vitro</i> for up to 96 hours and treated with each of four homeopathic preparations. The spectrophotometric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric Annexin V-PE/7-actinomycin D (7-AAD) and propidium iodide (PI) staining were each used to examine cell viability, apoptosis and cell cycle, respectively.</p><p><strong>Results: </strong> The MTT assay showed that all four homeopathic preparations reduced cell viability over time when compared to the control group cells, especially at 72 and 96 hours whereby only 50% of cells remained viable. Similarly, after 96 hours of treatment, the proportion of viable cells was significantly decreased and the proportion of early apoptotic (Annexin-V-PE +/7AAD-) cells was significantly increased for all four homeopathic preparations. Based on the PI-staining cell cycle data, cells treated with <i>Hecla lava</i> and <i>Carboneum sulphuratum</i> showed a statistically significant accumulation in the sub-G0/G1 phase of the cell cycle (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong> This is the first study to demonstrate that each of four homeopathic medicines causes apoptosis in a MM cell line. Further exploration of the potential of <i>Arsenicum album</i>, <i>Hecla lava</i>, <i>Carcinosinum</i> and <i>Carboneum sulphuratum</i> as a complementary therapeutic option in MM is warranted.</p>","PeriodicalId":13227,"journal":{"name":"Homeopathy","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}