Hormone Molecular Biology and Clinical Investigation最新文献_第9页

Ovarian tissue cryopreservation in Malaysia: a case series. 马来西亚卵巢组织冷冻保存:一个案例系列。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0096

Norazilah Mat Jin, Siti Maisarah Ahmad, Ahmad Mohd Faizal, Abdul Kadir Bin Abdul Karim, Muhammad Azrai Abu

{"title":"Ovarian tissue cryopreservation in Malaysia: a case series.","authors":"Norazilah Mat Jin, Siti Maisarah Ahmad, Ahmad Mohd Faizal, Abdul Kadir Bin Abdul Karim, Muhammad Azrai Abu","doi":"10.1515/hmbci-2021-0096","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0096","url":null,"abstract":"Objectives: We aim to discuss the hematological cancer cases that opted for ovarian tissue cryopreservation (OTC) as fertility preservation before the gonadotoxic chemotherapy agent.Case presentation: The ovarian tissue cryopreservation (OTC) was started in August 2020 in our center. Up to now, there were four cases have been performed and included in this report. The ovarian tissue cortex was cryopreserved with cryoprotectant using Kitazato™ (Tokyo, Japan) media and fit in the closed system devices. A total of four post-OTC patients were included. The mean age was 24 years old, whereas the mean serum AMH level was 30.43 pmol/L. Most of them were diagnosed with lymphoma, except one was leukemia. All of them received additional GnRH analog following OTC as a chemoprotective agent before cancer treatment. Currently, they are recovering well and on regular follow-up with the hematological department.Conclusions: Although The OTC is an ultimate option for prepubertal girls, it can be proposed as a good strategy for adult cancer women who could not delay cancer therapy.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The investigation of the frequency of the alpha-1-antitrypsin phenotype in patients with liver cirrhosis. 肝硬化患者α -1-抗胰蛋白酶表型频率的研究。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0001

Mehdi Afsharinasab, Amir Hossein Akbari, Vahid Mirzaei, Mehdi Mahmoodi, Mohammad Reza Hajizadeh, Jamal Amri, Alireza Khoshdel

{"title":"The investigation of the frequency of the alpha-1-antitrypsin phenotype in patients with liver cirrhosis.","authors":"Mehdi Afsharinasab, Amir Hossein Akbari, Vahid Mirzaei, Mehdi Mahmoodi, Mohammad Reza Hajizadeh, Jamal Amri, Alireza Khoshdel","doi":"10.1515/hmbci-2022-0001","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0001","url":null,"abstract":"Objectives: Alpha-1-antitrypsin (AAT) has different phenotypes. Evidence suggests that the abundance of each of these phenotypes may be associated with a disease. The purpose of this study was to evaluate the frequency of AAT phenotypes in patients with liver cirrhosis as well as in healthy individuals.Methods: In this study, 42 patients with liver cirrhosis were selected. The results of the previous research done by the researcher on healthy individuals were used to construct the control group. After obtaining informed consent, 5 mL of fasting venous blood sample was taken, and phenotypes were analyzed by isoelectric focusing. Data were analyzed using Chi-square and Fisher's exact tests at a significant level of 0.05.Results: The results of this study indicated that all 42 healthy subjects had an MM allele (100%). However, among 42 patients, 35 (83.3%) had an MM allele, 5 (11.9%) had an MS allele, and 2 (4.8%) had MZ allele. The difference between the two groups was significant (p=0.02). There was no difference between men and women in the allele type (p=0.557).Conclusions: This study revealed that MS and MZ alleles were observed only in patients with liver cirrhosis, and none of these alleles were found in healthy subjects. Therefore, MS and MZ alleles can be further investigated as risk factors for liver cirrhosis.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Esketamine-A quick-acting novel antidepressant without the disadvantages of ketamine. 艾氯胺酮-一种新型快速抗抑郁药，没有氯胺酮的缺点。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0099

Sheikh Shoib, Madhulika Kotra, Sana Javed, Vinh-Son Nguyen, Barikar C Malathesh

引用次数: 1

An overview of prognostic value of neurologic and cardiac biomarkers in patients with COVID-19 sequelae. 神经和心脏生物标志物对COVID-19后遗症患者预后的价值综述

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0015

Madhusudhan Umesh, Vidya Singaravelu, Vandana Daulatabad, Prafull Kamble, Anish Singhal, Nitin Ashok John, Jyoti John

{"title":"An overview of prognostic value of neurologic and cardiac biomarkers in patients with COVID-19 sequelae.","authors":"Madhusudhan Umesh, Vidya Singaravelu, Vandana Daulatabad, Prafull Kamble, Anish Singhal, Nitin Ashok John, Jyoti John","doi":"10.1515/hmbci-2022-0015","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0015","url":null,"abstract":"Many studies conducted after the pandemic period revealed that, while COVID-19 primarily injured the lungs, it also affects other organs in the form of cardiovascular complications, metabolic derangements, renal damage, and so on. Although we know that inflammatory cascades, complement activation, and pro-inflammatory cytokines are all involved in vasculitic processes that cause organ damage, we do not know the exact mechanism of complications such as acute respiratory distress syndrome (ARDS), cardiovascular ischemia, deep vein thrombosis, pulmonary thromboembolism, and brain injuries (embolism) that are frequently observed in COVID 19. The currently available biomarkers do not predict the severity of the aforementioned complications. As a result, more specific biomarkers such as serum calcium binding protein (S100B), glial fibrillary acid protein (GFAP), myelin basic protein (MBP), neuron-specific enolase (NSE), hs-TNI, (highly sensitive cardiac troponin) - HBDH, (Hydroxybutyrate Dehydrogenase), CK-MB (creatine kinase myocardial band), ST2 (suppression of tumorigenicity 2) are in need for early detection & improved clinical outcome.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10420988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Anti-oxidant effect of metformin through AMPK/SIRT1/PGC-1α/SIRT3- independent GPx1 expression in the heart of mice with endometriosis. 二甲双胍通过AMPK/SIRT1/PGC-1α/SIRT3独立的GPx1表达在子宫内膜异位症小鼠心脏中的抗氧化作用。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0039

Rodrigo Felgueiras, Ana C Neto, Adriana R Rodrigues, Alexandra M Gouveia, Henrique Almeida, Delminda Neves

{"title":"Anti-oxidant effect of metformin through AMPK/SIRT1/PGC-1α/SIRT3- independent GPx1 expression in the heart of mice with endometriosis.","authors":"Rodrigo Felgueiras, Ana C Neto, Adriana R Rodrigues, Alexandra M Gouveia, Henrique Almeida, Delminda Neves","doi":"10.1515/hmbci-2022-0039","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0039","url":null,"abstract":"Objectives: Endometriosis is a gynecological disease associated with an imbalance between oxidative species production and anti-oxidative defenses. In women, endometriosis has been reported to associate with increased incidence of cardiovascular events. As such, this study aimed to analyze the oxidation-responsive AMPK/SIRT1/PGC-1α/SIRT3 pathway in the heart of a mouse model of endometriosis. The effect of metformin, an insulin-sensitizing and anti-oxidative drug with already shown positive results in endometriotic tissue was studied.Methods: Thirty-six female B6CBA/F1 mice were divided into 4 groups (Control-C, Surgery-induced Endometriosis and Metformin-EM (50 mg/kg/day orally administrated for 3 months), Endometriosis-E and Metformin-M). Immunofluorescent labelling of SIRT1 and SIRT3 was performed in the heart tissue. Assessment of expression of AMPKα, SIRT1, PGC-1α, SIRT3, SOD2, and GPx1 was performed by Western Blotting. The quantification of microRNA(miR)-34a, miR-195, miR-217, miR-155 and miR-421, involved in the regulation of expression of SIRT1 and SIRT3, was performed by Real-Time PCR.Results: Data showed an increase in phospho-AMPKα and in GPx1 expression in the EM group when compared to the C group, but not in the total AMPK, SIRT1, PGC-1α, SIRT3 and SOD2, suggesting a GPx1 expression increase independently of the AMPK/SIRT1/PGC-1α/SIRT3 pathway. MicroRNAs, excepting miR-217, showed a consistent trend of increase in the M group.Conclusions: Our study showed that endometriosis does not significantly affect the expression of the components of the AMPK/SIRT1/PGC-1α/SIRT3 pathway in the heart. However, it indicates that an oxidative condition underlying endometriosis is required for metformin to evidence an increment in the expression of the anti-oxidative enzyme GPx1.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10476324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet. 高强度间歇训练对高脂高糖饮食大鼠血清和脂肪组织血管素水平的影响。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0107

Ali Delpisheh, Alireza Safarzade

{"title":"The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet.","authors":"Ali Delpisheh, Alireza Safarzade","doi":"10.1515/hmbci-2021-0107","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0107","url":null,"abstract":"Background: Vaspin is an adipocytokine with insulin-sensitizing and anti-inflammatory traits.Objectives: The purpose of this study was to evaluate the effect of high-intensity interval training (HIIT) on serum, visceral and subcutaneous adipose tissue vaspin levels in rats exposed to a diet high in fat and sugar (HFS).Materials and methods: Thirty-two male Wistar rats were randomly divided into HFS and standard diet (SD) groups. After 12 weeks, each group was divided into sedentary and HIIT groups. HIIT program was performed 3 times/week for 8 weeks. Retroperitoneal adipose tissue, inguinal adipose tissue and serum were collected to analyze vaspin levels. Also, serum glucose and insulin levels, insulin resistance index (HOMA-IR) and retroperitoneal and inguinal fat weights were measured.Results: HFS significantly increased weight gain, weight of inguinal (p=0.001) and retroperitoneal fat depots (p<0.001), serum glucose levels (p<0.001) and HOMA-IR (p<0.001). The HIIT was able to decline weight gain and fat mass (p<0.05) but did not affect inguinal and retroperitoneal fat depots' vaspin levels. Eight weeks' HIIT significantly increased serum vaspin (p=0.002) and decreased insulin (p=0.001) levels only in rats fed with SD.Conclusions: Although the HIIT program can cause significantly reducing effects on weight gain and fat depots' weights, it does not effect on circulating and fat depots' vaspin levels in rats fed an HFS.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10476325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients. 重症监护COVID-19患者缺氧诱导因子-1α和缺血修饰白蛋白水平

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0024

Kamile Yucel, Ali Fuat Gurbuz

{"title":"Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients.","authors":"Kamile Yucel, Ali Fuat Gurbuz","doi":"10.1515/hmbci-2022-0024","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0024","url":null,"abstract":"Objectives: In this study, it was aimed to evaluate the hypoxia-inducible factor-1α (HIF-1α) and ischemia-modified albumin (IMA) levels of patients diagnosed with COVID-19 in the intensive care unit (ICU) and healthy controls. To our knowledge, this is the first study investigate HIF-1α and IMA levels in COVID-19 patients in ICUs and comparing them with a healthy control group. For this reason, our study is original and will contribute to the literature.Methods: A total of 70 intensive care patients diagnosed with COVID-19, and 72 healthy controls were included in the study.Results: When we compared the patient and healthy control group; there were no statistically significant differences between the groups in terms of age and gender (p>0.05). No exitus was observed in the patient group. We found weak correlation between HIF-1α and IMA (r: 0.320). However, there were statistically significant differences in HIF-1α and IMA levels in the patient group. The receiver operating characteristic (ROC) curve demonstrated an area under curve (AUC) value of 0.651 for HIF-1α and 0.937 for IMA.Conclusions: The HIF-1α and IMA levels were significantly higher among COVID-19 patients in ICU compared with healthy controls. HIF-1α and IMA levels can be used as reliable markers for the prognosis of COVID-19.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10415238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats. 选择性雌激素受体α和β拮抗剂加重2型糖尿病去卵巢雌性大鼠心血管功能障碍。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0073

Hossein Azizian, Zeinab Farhadi, Mohammad Khaksari

{"title":"Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats.","authors":"Hossein Azizian, Zeinab Farhadi, Mohammad Khaksari","doi":"10.1515/hmbci-2021-0073","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0073","url":null,"abstract":"Objectives: Type 2 diabetes (T2D) is a major risk factor for cardiovascular disorders (CVD), characterized by pathological diastolic as well as systolic dysfunction, ventricular dilation, and cardiomyocyte hypertrophy. CVD is the main cause of death in postmenopausal women. Estradiol (E2) has protective effects on cardiovascular function. The biological effects of E2 are mainly mediated by classical estrogen receptors (ERs). The present study aimed to investigate the cardioprotective effects of classical ERs in ovariectomized (OVX) diabetic female rats.Methods: T2D was induced in female rats by high-fat diet feeding along with a low dose of streptozotocin. Then diabetic animals were divided into eight groups: Sham-control, OVX, OVX + Vehicle (Veh), OVX + E2, OVX + E2 + MPP (ERα antagonist), OVX + E2 + PHTPP (ERβ antagonist), OVX + E2 + Veh, OVX + E2 + MPP + PHTPP. Animals received E2, MPP, and PHTPP every four days for 28 days. At the end blood was collected, serum separated, and used for biochemical parameters. Heart tissue was used for cardiac angiotensin II and cytokines measurement.Results: E2 treatment improved the metabolic disorders caused by T2D, and its receptor antagonists intensified the effects of T2D on the metabolic status. Also, E2 therapy decreased cardiac inflammatory cytokines, and MPP and PHTPP increased cardiac inflammation by increasing TNF-α and IL-6 and decreasing IL-10.Conclusions: Classical ERs have protective effects on diabetic hearts by improving the metabolic status and inflammatory balance.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10450738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population. 希腊人群中TP53 Arg72Pro变异与复发性妊娠丢失之间的关系

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0093

Dimitra Dedousi, Despoina Mavrogianni, Myrto Papamentzelopoulou, Sofoklis Stavros, Rami Raouasnte, Dimitris Loutradis, Peter Drakakis

{"title":"Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population.","authors":"Dimitra Dedousi, Despoina Mavrogianni, Myrto Papamentzelopoulou, Sofoklis Stavros, Rami Raouasnte, Dimitris Loutradis, Peter Drakakis","doi":"10.1515/hmbci-2021-0093","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0093","url":null,"abstract":"Objectives: The present case-control study investigates whether TP53 Arg72Pro variant (rs1042522) serves as a risk factor for recurrent pregnancy loss (RPL) in Greek women.Methods: The study group consisted of 100 patients with at least two miscarriages of unexplained etiology, before the 24th week of gestation. The control group included 106 women with no pregnancy loss history. DNA was extracted and genotyped using specific primers for PCR amplification of the Arg72 and Pro72 alleles. Sanger sequencing was used for the discrimination between heterozygotes and homozygotes for Arg72Pro variant.Results: This is the first study demonstrating the statistically significant higher frequency of TP53 Arg72Pro variant in Greek RPL women compared to controls (38% vs. 6.6%; OR=8.6682, 95% CI: 3.6446-20.6160; p<0.0001). GC genotype (Arg/Pro) and CC genotype (Pro/Pro) were statistically more common in RPL patients than in controls (16% vs. 1.9%; p=0.0027, and 22 vs. 4.7%; p=0.0008, respectively). C allele frequency was statistically significant higher in RPL group than in controls (30.0 vs. 5.7%; p<0.0001). According to the inheritance mode analysis, the model that best fit the data was the dominant model (OR=8.67, 95% CI=3.64-20.62; p<0.0001).Conclusions: The is the first study disclosing strong evidence that TP53 rs1042522 is significantly associated with a higher risk for recurrent pregnancy loss in Greek women following a dominant model, thus, serving as a genetic marker for identifying women at increased risk of recurrent miscarriages.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10408017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6). 鉴别肝癌的潜在肿瘤标志物:PI3K和促炎细胞因子(TGF-β、IL-1和IL-6)。

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Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0028

Fahimeh Tabakhiyan, Amirabbas Mir, Vahid Vahedian

{"title":"Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6).","authors":"Fahimeh Tabakhiyan, Amirabbas Mir, Vahid Vahedian","doi":"10.1515/hmbci-2022-0028","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0028","url":null,"abstract":"Objectives: Hepatocellular carcinoma (HCC), the most common form of liver cancer, is a leading cause of tumor-associated mortality worldwide. Diagnosis based upon non-invasive criteria is currently challenged by the need for molecular information that requires tissue or liquid biopsies. The progression of HCC is often associated with chronic inflammation, expression levels of inflammatory mediators, chemokine, and cytokines. In this study, we try to evaluate the PI3K and pro-inflammatory cytokines, TGF-β, IL-1, and IL-6 expression level in patients with liver cancer.Materials and methods: The kupffer cells were isolated from patient's specimens. Real-time PCR was applied to evaluate the expression level of PI3K in cell lines or tumors. The concentrations of TGF-β, IL-1, and IL-6 were measured by the quantitative ELISA kit.Results: PI3K mRNA expression in cancer cells was increased markedly vs. normal cells. The ELISA results demonstrated over expression of TGF-β, IL-1, and IL-6 in patients and positive correlation between tumor size and stage.Discussion: This study suggests that targeting the expression level of PI3K and pro-inflammatory chemokine and cytokines, TGF-β, IL-1, and IL-6, may be a potential diagnostic strategy in HCC patients.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1