Hormone Molecular Biology and Clinical Investigation最新文献_第10页

Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients. 重症监护COVID-19患者缺氧诱导因子-1α和缺血修饰白蛋白水平

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0024

Kamile Yucel, Ali Fuat Gurbuz

{"title":"Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients.","authors":"Kamile Yucel, Ali Fuat Gurbuz","doi":"10.1515/hmbci-2022-0024","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0024","url":null,"abstract":"Objectives: In this study, it was aimed to evaluate the hypoxia-inducible factor-1α (HIF-1α) and ischemia-modified albumin (IMA) levels of patients diagnosed with COVID-19 in the intensive care unit (ICU) and healthy controls. To our knowledge, this is the first study investigate HIF-1α and IMA levels in COVID-19 patients in ICUs and comparing them with a healthy control group. For this reason, our study is original and will contribute to the literature.Methods: A total of 70 intensive care patients diagnosed with COVID-19, and 72 healthy controls were included in the study.Results: When we compared the patient and healthy control group; there were no statistically significant differences between the groups in terms of age and gender (p>0.05). No exitus was observed in the patient group. We found weak correlation between HIF-1α and IMA (r: 0.320). However, there were statistically significant differences in HIF-1α and IMA levels in the patient group. The receiver operating characteristic (ROC) curve demonstrated an area under curve (AUC) value of 0.651 for HIF-1α and 0.937 for IMA.Conclusions: The HIF-1α and IMA levels were significantly higher among COVID-19 patients in ICU compared with healthy controls. HIF-1α and IMA levels can be used as reliable markers for the prognosis of COVID-19.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"415-420"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10415238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats. 选择性雌激素受体α和β拮抗剂加重2型糖尿病去卵巢雌性大鼠心血管功能障碍。

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0073

Hossein Azizian, Zeinab Farhadi, Mohammad Khaksari

{"title":"Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats.","authors":"Hossein Azizian, Zeinab Farhadi, Mohammad Khaksari","doi":"10.1515/hmbci-2021-0073","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0073","url":null,"abstract":"Objectives: Type 2 diabetes (T2D) is a major risk factor for cardiovascular disorders (CVD), characterized by pathological diastolic as well as systolic dysfunction, ventricular dilation, and cardiomyocyte hypertrophy. CVD is the main cause of death in postmenopausal women. Estradiol (E2) has protective effects on cardiovascular function. The biological effects of E2 are mainly mediated by classical estrogen receptors (ERs). The present study aimed to investigate the cardioprotective effects of classical ERs in ovariectomized (OVX) diabetic female rats.Methods: T2D was induced in female rats by high-fat diet feeding along with a low dose of streptozotocin. Then diabetic animals were divided into eight groups: Sham-control, OVX, OVX + Vehicle (Veh), OVX + E2, OVX + E2 + MPP (ERα antagonist), OVX + E2 + PHTPP (ERβ antagonist), OVX + E2 + Veh, OVX + E2 + MPP + PHTPP. Animals received E2, MPP, and PHTPP every four days for 28 days. At the end blood was collected, serum separated, and used for biochemical parameters. Heart tissue was used for cardiac angiotensin II and cytokines measurement.Results: E2 treatment improved the metabolic disorders caused by T2D, and its receptor antagonists intensified the effects of T2D on the metabolic status. Also, E2 therapy decreased cardiac inflammatory cytokines, and MPP and PHTPP increased cardiac inflammation by increasing TNF-α and IL-6 and decreasing IL-10.Conclusions: Classical ERs have protective effects on diabetic hearts by improving the metabolic status and inflammatory balance.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"427-436"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10450738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6). 鉴别肝癌的潜在肿瘤标志物:PI3K和促炎细胞因子(TGF-β、IL-1和IL-6)。

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0028

Fahimeh Tabakhiyan, Amirabbas Mir, Vahid Vahedian

{"title":"Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6).","authors":"Fahimeh Tabakhiyan, Amirabbas Mir, Vahid Vahedian","doi":"10.1515/hmbci-2022-0028","DOIUrl":"https://doi.org/10.1515/hmbci-2022-0028","url":null,"abstract":"Objectives: Hepatocellular carcinoma (HCC), the most common form of liver cancer, is a leading cause of tumor-associated mortality worldwide. Diagnosis based upon non-invasive criteria is currently challenged by the need for molecular information that requires tissue or liquid biopsies. The progression of HCC is often associated with chronic inflammation, expression levels of inflammatory mediators, chemokine, and cytokines. In this study, we try to evaluate the PI3K and pro-inflammatory cytokines, TGF-β, IL-1, and IL-6 expression level in patients with liver cancer.Materials and methods: The kupffer cells were isolated from patient's specimens. Real-time PCR was applied to evaluate the expression level of PI3K in cell lines or tumors. The concentrations of TGF-β, IL-1, and IL-6 were measured by the quantitative ELISA kit.Results: PI3K mRNA expression in cancer cells was increased markedly vs. normal cells. The ELISA results demonstrated over expression of TGF-β, IL-1, and IL-6 in patients and positive correlation between tumor size and stage.Discussion: This study suggests that targeting the expression level of PI3K and pro-inflammatory chemokine and cytokines, TGF-β, IL-1, and IL-6, may be a potential diagnostic strategy in HCC patients.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"389-396"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population. 希腊人群中TP53 Arg72Pro变异与复发性妊娠丢失之间的关系

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0093

Dimitra Dedousi, Despoina Mavrogianni, Myrto Papamentzelopoulou, Sofoklis Stavros, Rami Raouasnte, Dimitris Loutradis, Peter Drakakis

{"title":"Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population.","authors":"Dimitra Dedousi, Despoina Mavrogianni, Myrto Papamentzelopoulou, Sofoklis Stavros, Rami Raouasnte, Dimitris Loutradis, Peter Drakakis","doi":"10.1515/hmbci-2021-0093","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0093","url":null,"abstract":"Objectives: The present case-control study investigates whether TP53 Arg72Pro variant (rs1042522) serves as a risk factor for recurrent pregnancy loss (RPL) in Greek women.Methods: The study group consisted of 100 patients with at least two miscarriages of unexplained etiology, before the 24th week of gestation. The control group included 106 women with no pregnancy loss history. DNA was extracted and genotyped using specific primers for PCR amplification of the Arg72 and Pro72 alleles. Sanger sequencing was used for the discrimination between heterozygotes and homozygotes for Arg72Pro variant.Results: This is the first study demonstrating the statistically significant higher frequency of TP53 Arg72Pro variant in Greek RPL women compared to controls (38% vs. 6.6%; OR=8.6682, 95% CI: 3.6446-20.6160; p<0.0001). GC genotype (Arg/Pro) and CC genotype (Pro/Pro) were statistically more common in RPL patients than in controls (16% vs. 1.9%; p=0.0027, and 22 vs. 4.7%; p=0.0008, respectively). C allele frequency was statistically significant higher in RPL group than in controls (30.0 vs. 5.7%; p<0.0001). According to the inheritance mode analysis, the model that best fit the data was the dominant model (OR=8.67, 95% CI=3.64-20.62; p<0.0001).Conclusions: The is the first study disclosing strong evidence that TP53 rs1042522 is significantly associated with a higher risk for recurrent pregnancy loss in Greek women following a dominant model, thus, serving as a genetic marker for identifying women at increased risk of recurrent miscarriages.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"421-426"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10408017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19 pandemic: transmission of SARS-CoV-2 in animals, a risk for human beings. COVID-19大流行:SARS-CoV-2在动物中传播，对人类构成风险。

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0109

Ashok Kumar Ahirwar, Kirti Kaim, Pradeep Ahirwar, Jitender Prasad

引用次数: 0

Potential role of melatonin in prevention and treatment of lung cancer. 褪黑素在防治肺癌中的潜在作用。

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2022-0018

Zi Ni Ngai, Kian Chung Chok, Khuen Yen Ng, Rhun Yian Koh, Soi Moi Chye

引用次数: 3

Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis. 姜黄素通过rek /基质金属蛋白酶轴的表观遗传重编程对A-549肺癌细胞的抗迁移作用

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-12-01 DOI: 10.1515/hmbci-2021-0100

Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi

{"title":"Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis.","authors":"Shabnam Mostofi, Dariush Shanehbandi, Seyyed Ali Rahmani, Milad Asadi","doi":"10.1515/hmbci-2021-0100","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0100","url":null,"abstract":"Objectives: The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, RECK/MMPs axis as a probable regulator of cancer cell migration was assessed.Methods: In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of RECK gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including CASP3, CASP8, CASP9, BAX, BCL2, MMP9, MMP2, and RECK) were investigated by quantitative Real-Time PCR.Results: The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the RECK gene promoter after curcumin treatment.Conclusions: Curcumin inhibited lung cancer cells through various molecular pathways. RECK/MMPs axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"455-461"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10476859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Endometriosis at all ages: diagnostic ultrasound 所有年龄段的子宫内膜异位症：超声诊断

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-06-01 DOI: 10.1515/hmbci-2021-0082

M. Bäumler, Niko Heiss, R. Druckmann

引用次数: 1

Oxytocin, the panacea for long-COVID? a review 催产素，治疗长期新冠肺炎的灵丹妙药？评论

IF 1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-05-16 DOI: 10.1515/hmbci-2021-0034

P. Diep, M. Chaudry, Adam Dixon, Faisal Chaudry, V. Kasabri

{"title":"Oxytocin, the panacea for long-COVID? a review","authors":"P. Diep, M. Chaudry, Adam Dixon, Faisal Chaudry, V. Kasabri","doi":"10.1515/hmbci-2021-0034","DOIUrl":"https://doi.org/10.1515/hmbci-2021-0034","url":null,"abstract":"Abstract Objectives In this hypothesis paper we explore the underlying mechanisms for long-COVID and how the oxytocinergic neurones could be infected by SARS-CoV-2 leading to a reduction in plasma oxytocin (OXT). Furthermore, we aim to review the relevance of OXT and hypothalamic function in recovery from long-COVID symptoms and pathology, through exploring the pro-health effects of the OXT neuropeptide. Methods A review of published literature was surveyed using Google Scholar and PubMed. Results Numerous experimental data can be shown to correlate with OXT and long-COVID symptoms and conditions, thus providing strong circumstantial evidence to support our hypothesis. It is postulated that the reduction in plasma OXT due to acute and post-viral damage to the hypothalamus and oxytocinergic neurones contributes to the variable multi-system, remitting and relapsing nature of long-COVID. The intranasal route of OXT application was determined to be most appropriate and clinically relevant for the restoration of oxytocinergic function post COVID-19 infection. Conclusions We believe it is imperative to further investigate whether OXT alleviates the prolonged suffering of patients with long-COVID. Succinctly, OXT may be the much-needed post-pandemic panacea. Highlights – A comparison of long-COVID pathology with OXT therapeutic mechanisms was performed – Following advances in neuroendocrinology and proposed use of OXT as an acute antiviral for COVID-19; we elaborate OXT mechanisms in treating long-COVID, via therapeutic relevance and methods of administration. – Further demonstration of OXT levels for different demographic and susceptibility groups, acute levels in COVID-19 patients and periodic monitoring of OXT levels is warranted.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 1","pages":"363 - 371"},"PeriodicalIF":1.0,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46792580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anti Mullerian hormone as a diagnostic tool for polycystic ovary syndrome in women of reproductive age with morbid obesity. 将抗穆勒氏管激素作为病态肥胖育龄妇女多囊卵巢综合征的诊断工具。

IF 1.1

Hormone Molecular Biology and Clinical Investigation Pub Date : 2022-05-05 eCollection Date: 2022-12-01 DOI: 10.1515/hmbci-2021-0078

Ibtissem Oueslati, Mohamed Bassem Hammami, Seif Boukriba, Hana Ben Hadj Hassen, Meriem Yazidi, Fatma Chaker, Habiba Mizouni, Moncef Feki, Melika Chihaoui

{"title":"Anti Mullerian hormone as a diagnostic tool for polycystic ovary syndrome in women of reproductive age with morbid obesity.","authors":"Ibtissem Oueslati, Mohamed Bassem Hammami, Seif Boukriba, Hana Ben Hadj Hassen, Meriem Yazidi, Fatma Chaker, Habiba Mizouni, Moncef Feki, Melika Chihaoui","doi":"10.1515/hmbci-2021-0078","DOIUrl":"10.1515/hmbci-2021-0078","url":null,"abstract":"Objectives: The aim of the study was to analyze the performance of the anti-mullerian hormone (AMH) level for the diagnosis of polycystic ovary syndrome in women with morbid obesity.Study design: A single-centre cross-sectional study was conducted in 50 women of reproductive age with a body mass index (BMI) ≥ 40 kg/m2. Each patient underwent a clinical examination, biological and hormonal assays, and an ovarian ultrasound between the third and the fifth day of the menstrual cycle. Polycystic ovary syndrome was diagnosed according to the Rotterdam's criteria.Results: The mean age of participants was 34.2 ± 7.5 years. Polycystic ovary syndrome was diagnosed in 20 women (40%). Age and anthropometric parameters did not differ between women with and without polycystic ovary syndrome. The mean AMH level was significantly higher in women with polycystic ovary syndrome (3.4 ± 3.6 vs 1.3 ± 1.2 ng/ml, p=0.010). It was positively correlated with the Ferriman and Gallwey score (r=0.496, p=0.016), total testosterone level (r=0.524, p < 10-3) and the LH/FSH ratio (r=0.290, p=0.046). In women aged between 35 and 45 years, the optimum cut-off level for the diagnosis of polycystic ovary syndrome was 0.81 ng/mL, providing a sensitivity and a specificity of 90 and 71%, respectively with an area under the ROC curve of 0.857.Conclusions: AMH level was significantly higher in morbid obese women with polycystic ovary syndrome compared with those without polycystic ovary syndrome. Specific thresholds for this population must be assessed to improve the sensitivity and specificity of AMH for the diagnosis of polycystic ovary syndrome.","PeriodicalId":13224,"journal":{"name":"Hormone Molecular Biology and Clinical Investigation","volume":"43 4","pages":"381-387"},"PeriodicalIF":1.1,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10450735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0