The association of paraoxonase I gene polymorphisms Q192R (rs662) and L55M (rs854560) and its activity with metabolic syndrome components in fars ethnic group.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-02-17 eCollection Date: 2023-09-01 DOI:10.1515/hmbci-2022-0064

Abdoljalal Marjani, Nahid Poursharifi, Mohammad Mostakhdem Hashemi, Atefe Sajedi, Mahin Tatari

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引用次数: 0

Abstract

Objectives: Metabolic syndrome (MetS) may cause premature development of some diseases. PON1 genes may be involved in the pathogenesis of MetS. The aim of study was to evaluate the association between Q192R and L55M gene polymorphisms and its enzyme activity with the MetS components in subjects with and without MetS.

Methods: Polymerase Chain Reaction and Restriction Fragment Length Polymorphism analysis were performed to determine polymorphisms of the paraoxonase1 gene in subjects with and without metabolic syndrome. Biochemical parameters were measured by spectrophotometer.

Results: The MM, LM, and LL genotype frequencies of the PON1 L55M polymorphism were 10.5, 43.4, and 46.1%, and 22.4, 46.6, and 31% and; the QQ, QR, and RR genotype frequencies of the PON1 Q192R polymorphism were 55.4, 38.6 and 6%; and 56.5, 34.8 and 8.7% in subjects with and without MetS, respectively. The L and M allele frequencies were 68 and 53%; and 32 and 47% for PON1 L55M in subjects with and without MetS, respectively. The Q and R allele frequencies for PON1 Q192R were 74 and 26% in both groups. There were significant differences in HDL-cholesterol level and PON1 activity in the genotypes QQ, QR, and RR of the PON1 Q192R polymorphism in subjects with MetS.

Conclusions: The PON1 Q192R genotypes had only effected on PON1 activity and HDL-cholesterol level in subjects with MetS. Different genotypes of the PON1 Q192R seem to be important candidates to make the subjects susceptible to MetS in the Fars ethnic group.

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对氧磷酶I基因多态性Q192R（rs662）和L55M（rs854560）及其活性与法尔斯族代谢综合征成分的相关性。

目的：代谢综合征（MetS）可能导致某些疾病的过早发展。PON1基因可能参与MetS的发病机制。本研究的目的是评估患有和不患有代谢综合征的受试者中Q192R和L55M基因多态性及其酶活性与代谢综合征成分之间的关系。用分光光度计测定生化参数。结果：PON1 L55M多态性的MM、LM和LL基因型频率分别为10.5%、43.4%和46.1%，22.4%、46.6%和31%；PON1 Q192R多态性的QQ、QR和RR基因型频率分别为55.4%、38.6%和6%；患有和不患有MetS的受试者分别为56.5%、34.8%和8.7%。L和M等位基因频率分别为68%和53%；在患有和不患有MetS的受试者中PON1 L55M分别为32%和47%。PON1 Q192R的Q和R等位基因频率在两组中分别为74%和26%。MetS患者PON1 Q192R多态性基因型QQ、QR和RR的HDL胆固醇水平和PON1活性存在显著差异。PON1 Q192R的不同基因型似乎是使法尔斯族受试者易感MetS的重要候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.