IBRO Neuroscience Reports最新文献

{"title":"Pupillary ultrasonography vs Horner's syndrome to evaluate efficacy of stellate ganglion block in patients","authors":"Zhu-Hong Fang ,&nbsp;Yong Ni ,&nbsp;Hong Xie ,&nbsp;Hai-Long Zhang ,&nbsp;Jia-Xuan Yang","doi":"10.1016/j.ibneur.2025.07.007","DOIUrl":"10.1016/j.ibneur.2025.07.007","url":null,"abstract":"<div><div>Stellate ganglion block (SGB) is not only used for treating cervical thoracic, and upper limb pain-related diseases but also for treating sympathetic nervous system dysfunction-related diseases, and it is a widely used clinical treatment method. This study has demonstrated that utilizing ultrasound to measure pupillary diameter (PD) changes and significant inter-eye differences offers a more objective assessment of stellate ganglion block (SGB) efficacy compared to the traditional subjective evaluation based on Horner’s syndrome (HS). However, there is currently no objective standard to evaluate the efficacy of SGB. Pupillary ultrasonography can observe precise dynamic changes in the pupils, and is less affected by peripheral light, non-invasive, and convenient. We performed SGB under ultrasound guidance, and evaluated the effectiveness of SGB according to HS in 60 patients. All patients were scored using a visual analogue scale (VAS) before and 24 h after stellate ganglion block treatment. We compared the changes in pupil diameter (PD) and pupillary constriction ratio during pupil light reflex (PLR) in the blocked side and the opposite side. Then we calculated the inter-eye difference of PD before and after SGB, and the inter-eye difference of PD in the blocked side and the opposite side (BO-IED) at each time point. We found that the area under the curve (AUC) for evaluating the effectiveness of SGB was statistically significant at each time points for BO-IED and blocked side inter-eye difference. Additionally, the BO-IED at 15 min after SGB has the largest AUC, when the BO-IED is greater than 0.5 mm, which shows a sensitivity of 86.5 %, and a specificity of 100 %. Our findings suggested an association between pupil reduction and symptom reduction in patients, with the degree of pupil reduction serving as a reference for evaluating the effectiveness of SGB and the degree of symptom relief.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 264-271"},"PeriodicalIF":2.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and quantification of GABAA R-α1-positive cells in the DCN of rats with behavioral evidence of noise-induced tinnitus","authors":"Xiaoping Du,&nbsp;Jianzhong Lu,&nbsp;Zachary Yokell,&nbsp;Weihua Cheng,&nbsp;Don Nakmali,&nbsp;Richard D. Kopke,&nbsp;Matthew B. West","doi":"10.1016/j.ibneur.2025.07.005","DOIUrl":"10.1016/j.ibneur.2025.07.005","url":null,"abstract":"<div><div>Tinnitus, the perception of a phantom sound, often occurs as a clinical sequela of auditory traumas. However, the underlying mechanisms of tinnitus are largely unknown. In our previous studies, we found more gamma-aminobutyric acid A receptor alpha 1 subunit (GABA_AR-α1)-positive cells in the dorsal cochlear nucleus (DCN) after noise exposure, however, we were not able to identify the specific types of DCN cells that up-regulated GABA_AR-α1 after the insult. In the current study, we used Nissl staining, Purkinje cell protein 4 (PCP4) and glutamic acid decarboxylase 67 (GAD67) immunolabeling to identify GABA_AR-α1-positive cells in the DCN. Each type of GABA_AR-α1-positive cells was quantified and statistically analyzed using immunostaining and Nissl staining according to their morphology, size and location in the DCN. GABA_AR-α1-positive cartwheel cells, Golgi cells, as well as ML-stellate and vertical cells were confirmed by dual immunolabeling. In the DCN, the most common GABA_AR-α1-positive cells were Golgi cells followed by vertical cells and cartwheel cells while very few other cells were GABA_AR-α1-positive in all conditions. We found significantly more GABA_AR-α1-positive Golgi cells in the DCN of noise-exposed rats without behavioral evidence of tinnitus compared to normal controls and noise-exposed rats with behavioral evidence of tinnitus. This heightened “context-dependent” inhibition may help to maintain a balanced neuronal network, preventing the potential for tinnitus-related hyperactivity in the auditory pathways.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 332-344"},"PeriodicalIF":2.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The nitrergic mechanism of geraniol in PTZ-induced seizures","authors":"Babak Shahhosseini ,&nbsp;Hossein Tahmasebi Dehkordi ,&nbsp;Hossein Amini-Khoei ,&nbsp;Antoni Sureda ,&nbsp;Mehrdad Shahrani ,&nbsp;Zahra Lorigooini","doi":"10.1016/j.ibneur.2025.07.004","DOIUrl":"10.1016/j.ibneur.2025.07.004","url":null,"abstract":"<div><h3>Background</h3><div>This investigation aims to elucidate the role of NO in the anticonvulsant effects of Geraniol (GER) using a mouse model of pentylenetetrazole (PTZ)-induced seizures.</div></div><div><h3>Methods</h3><div>Mice were allocated into ten groups, including a control group receiving normal saline. The treatment groups received GER (10, 20, 30, and 40 mg/kg), L-NAME (10 mg/kg), L-arginine (L-arg) at 150 mg/kg, a sub-effective dose of GER (10 mg/kg) combined with L-NAME, and an effective dose of GER (40 mg/kg) plus L-arg, respectively. All drugs were administered <em>intraperitoneally</em> 30 min before seizure induction by <em>intravenous</em> infusion of PTZ. The last group served as the control for biochemical and molecular tests (no seizure induction). Subsequently, the seizure threshold was recorded. Nitrite levels in serum and the prefrontal cortex (PFC), as well as the gene expression of nNOS and iNOS in the PFC, were assessed.</div></div><div><h3>Results</h3><div>GER prolonged the seizure threshold and reduced serum and PFC nitrite levels. Also, it downregulated the gene expression of <em>nNOS</em> and <em>iNOS</em>. Simultaneous administration of L-arg with the effective GER dose (40 mg/kg) notably reversed the beneficial effects of GER. Conversely, when administered with a sub-effective dose of GER (10 mg/kg), L-NAME potentiated the effects of this dose of GER. The expression of the <em>nNOS</em> gene in the PFC significantly increased following the administration of 20 mg/kg GER and L-arg plus 40 mg/kg GER. Conversely, 40 mg/kg GER alone reduced <em>nNOS</em> gene expression in the PFC.</div></div><div><h3>Conclusion</h3><div>GER exhibits anticonvulsant properties by modulating the nitrergic system, increasing seizure latency, and reducing NO production. This suggests its potential as a therapeutic candidate for seizure management.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 257-263"},"PeriodicalIF":2.0,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the role of omega-3 fatty acids in nicotine-induced neurotoxicity in pregnant Wistar rats and their pups","authors":"Joseph Gbenga Omole ,&nbsp;Quadri Kunle Alabi ,&nbsp;Mayowa Grace Elemile ,&nbsp;Ayodeji Aturamu ,&nbsp;Modinat Adebukola Adefisayo ,&nbsp;Gbenga Olatunde Omotosho ,&nbsp;Samson Mokolade Ige ,&nbsp;Orji Eunice Ibiye ,&nbsp;Tokunbo Olorunfemi Samuel ,&nbsp;Fisayo Nathaniel Ogunleye ,&nbsp;Peace Kehinde Olamilosoye ,&nbsp;Oladele Abiodun Ayoka","doi":"10.1016/j.ibneur.2025.07.003","DOIUrl":"10.1016/j.ibneur.2025.07.003","url":null,"abstract":"<div><div>This study investigated maternal and their pup neurobehaviour following nicotine exposure during gestational and postnatal periods and neuroprotective effect of Omega-3 fatty acids. Thus, the study used thirty pregnant female Wistar rats for this study. Groups I and II (nonpregnant and pregnant) were treated with 1 ml/kg/day of normal saline for 42 days; III was treated with 4 mg/kg/day of nicotine for 42 days; IV-VI were co-administered nicotine 4 mg/kg and 100, 300, 600 mg/kg/day of Omega-3 fatty acids respectively for 42 days. Results: The beam walk time of the mother rats in groups III and IV were significantly higher when compared with other groups. Similarly, the beam walks time of the pups of groups III and IV were significantly higher when compared with the pups of the mother rats in other groups. The brain dopamine and serotonin levels of mother rats in groups III, IV and V were significantly higher when compared with other groups. Also, the brain dopamine and serotonin levels of the pups in groups III and IV were significantly higher when compared with the pups in other groups. The reduced glutathione and catalase of mother and pup rats in groups III and IV were significantly lower when compared with other groups. Photomicrographs of cerebellum and hippocampus of the rats treated with nicotine showed scattered arrangement of pyramidal cells with vacuolated neurons. These alterations were significantly reversed with Omega-3 fatty acids following nicotine exposure. Conclusions. Omega-3 fatty acids at 300 and 600 mg/kg ameliorated nicotine-induced neurotoxicity in mother rats and their pups.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 272-289"},"PeriodicalIF":2.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long COVID is associated with female sex; Anti-NCAM1 autoantibodies are absent in patients with long COVID","authors":"Yukiko Motokawa ,&nbsp;Jun Sugihara ,&nbsp;Tomoya Tateishi ,&nbsp;Tadashi Hosoya ,&nbsp;Shinsuke Yasuda ,&nbsp;Yasunari Miyazaki ,&nbsp;Hidehiko Takahashi ,&nbsp;Hiroki Shiwaku","doi":"10.1016/j.ibneur.2025.07.002","DOIUrl":"10.1016/j.ibneur.2025.07.002","url":null,"abstract":"<div><h3>Background</h3><div>Long COVID is a condition that may arise following SARS-CoV-2 infection and is associated with a range of systemic complications. Autoantibodies are implicated in the pathogenesis of long COVID. However, the details of the pathogenic mechanisms undergone by these autoantibodies remain unclear. Neural cell adhesion molecule 1 (NCAM1) is the human protein with the highest sequence homology to the SARS-CoV-2 proteins. Previous in silico studies indicate that SARS-CoV-2 infection may induce the production of anti-NCAM1 autoantibodies. Thus, this study investigated the presence of anti-NCAM1 autoantibodies in individuals affected by COVID-19, including those with long COVID.</div></div><div><h3>Methods</h3><div>Serum samples were obtained from 173 individuals 3 months after SARS-CoV-2 infection. Among them, 63 were diagnosed with long COVID. A cell-based assay was used to assess all 173 serum samples for the presence of anti-NCAM1 autoantibodies. We also analyzed the clinical profiles of patients with and without long COVID to identify potential risk factors associated with long COVID.</div></div><div><h3>Results</h3><div>Anti-NCAM1 autoantibodies were not detected in any serum sample. The proportion of female patients in the long COVID group was significantly higher than that in the non-long COVID group.</div></div><div><h3>Conclusion</h3><div>The results indicate that the production of anti-NCAM1 autoantibodies following COVID-19 is unlikely. Female sex is associated with higher risk of long COVID.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 252-256"},"PeriodicalIF":2.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olfactory training in specific anosmia to androstenone and its association with genetic variations of OR7D4","authors":"Antonia Gillmeister ,&nbsp;Maira Harume Nagai ,&nbsp;Christian Margot ,&nbsp;Priyanka Meesa ,&nbsp;Hiroaki Matsunami ,&nbsp;Thomas Hummel","doi":"10.1016/j.ibneur.2025.06.017","DOIUrl":"10.1016/j.ibneur.2025.06.017","url":null,"abstract":"<div><div>Short-term, repeated exposure to odors, “olfactory training” (OT), improves olfactory function. Clinically, this works not only for trauma- or disease-related olfactory impairment but also in people with specific anosmia. Androstenone is an odorant for which the frequent occurrence of specific anosmia is already known. It is an odorous steroid derived from testosterone. Besides some people who cannot perceive the odor, it is perceived differently by different individuals in terms of odor quality. These differences in the ability to perceive androstenone as well as in the perception of its quality were previously related to single nucleotide polymorphisms of the human olfactory receptor OR7D4. The current study addressed the question of whether changes in the perception of androstenone in relation to a change in sensitivity following specific OT with that odorant are associated with genetic variations of OR7D4. A total of 335 healthy volunteers participated (206 females, 129 males). All participants underwent tests for normal olfactory function; 103 showed specific anosmia for androstenone. Seventy-seven participants initially unable to perceive androstenone performed OT for an average duration of 8 weeks. Detection thresholds as well as subjective evaluation of odor intensity and pleasantness were measured both before and after OT. Buccal swabs were taken to examine the OR7D4 genotype. The study provided the following major results: (1) Detection thresholds were significantly lower after OT. (2) There was no statistically significant impact of the OR7D4 genotype on the ability to perceive androstenone after OT. In conclusion, it appears that the ability to perceive androstenone can be trained in people with specific anosmia, although OR7D4 polymorphisms were not related to a major change in the sensitivity towards androstenone.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 245-251"},"PeriodicalIF":2.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of AKT-mediated p27Kip1 phosphorylation with shift to sphingomyelin synthesis in CLN3 disease","authors":"Fatima Bilal ,&nbsp;Jihane Soueid ,&nbsp;Sara Saab ,&nbsp;Nadine Makhoul ,&nbsp;Zeinab Hamze ,&nbsp;Lara El-Bazzal ,&nbsp;Joelle Makoukji ,&nbsp;Rose-Mary Boustany","doi":"10.1016/j.ibneur.2025.06.005","DOIUrl":"10.1016/j.ibneur.2025.06.005","url":null,"abstract":"<div><div>CLN3 disease is a fatal childhood neurodegenerative disorder without drug-modifying therapies. Wild-type <em>CLN3</em> gene is anti-apoptotic. Previous work proves that CLN3 disease pathogenesis is associated with reduced cell viability/apoptotic cell death and increase in ceramide, in cells and brains of patients and in <em>Cln3</em>^{<em>Δex7/8</em>} mouse brain. AKT, a serine/threonine protein kinase, is at the core of a complex signaling pathway regulating apoptosis and cell proliferation and is neuroprotective. AKT fulfils this function via cyclin-dependent kinase inhibitor p27^Kip1 phosphorylation at residue T198 resulting in its cytoplasmic localization and promotion of cell survival. Transcriptomic analysis of gene expression using microarrays, validation by Western blot and real-time PCR, demonstrate that AKT is downregulated in CLN3-deficient cells and <em>Cln3</em>^{<em>Δex7/8</em>} mouse brain. Conversely, CLN3 overexpression causes upregulation of AKT proteins. Novel data shows that CLN3 deficiency reduces AKT-mediated p27^Kip1 T198 phosphorylation, possibly leading to reduced cell viability. Dysregulation in AKT/p27^Kip1 signaling alters sphingolipid metabolism. Results confirm an increase in sphingomyelin synthesis in CLN3-deficient cells.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 223-234"},"PeriodicalIF":2.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined effects of motor imagery and motor practice are influenced by differences in working memory function: Examination of brain, spinal cord, and muscle performance data","authors":"Yuki Fukumoto ,&nbsp;Hiroki Bizen ,&nbsp;Marina Todo ,&nbsp;Naoki Yoshida ,&nbsp;Toshiaki Suzuki","doi":"10.1016/j.ibneur.2025.06.016","DOIUrl":"10.1016/j.ibneur.2025.06.016","url":null,"abstract":"<div><div>The ability to perform motor imagery is affected by differences in short-term memory storage capacity in terms of the activation of working memory. Therefore, from the viewpoint of the simultaneous measurement of brain activation and spinal motor neuron excitability, we examined differences in the combined effects of motor practice and motor imagery due to differences in working memory function. 20 healthy individuals were classified into Normal (score of ≤5–6 digits) and Good (score of ≥7 digits) groups based on working memory in a digit span test. Following this, participants performed six sets of repetitive exercises combining motor practice and motor imagery, and changes in neural activity patterns in the brain and spinal cord, as well as changes in finger dexterity, were tracked. In brain network analysis, the first execution of the imagery showed high Betweenness Centrality in the frontal pole cortex, which shifted to the dorsolateral prefrontal cortex with repeated imagery. The involvement of the frontal pole may reflect introspection of motor behavior in the initial stage, while the dorsolateral prefrontal cortex consistently participated in imagery generation throughout the entire motor imagery process. In addition, both groups showed improvement in finger dexterity after intervention, but during repetitive motor imagery, a decrease in amplitude F/M ratio was observed in the Good Working Memory group, and a decrease in activation of the right primary motor cortex was observed in the Normal Working Memory group. In terms of working memory, especially in aspects of the phonological loop, those with higher function may execute motor imagery more distinctly.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 210-222"},"PeriodicalIF":2.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophysiological analysis of distal, proximal, and dual compression of the median nerve","authors":"Qiongfang Zhang ,&nbsp;Yongfeng Liu ,&nbsp;Jinhuan Zhang ,&nbsp;Yirong Chen ,&nbsp;Xingxian Huang ,&nbsp;Haibo Yu","doi":"10.1016/j.ibneur.2025.06.010","DOIUrl":"10.1016/j.ibneur.2025.06.010","url":null,"abstract":"<div><h3>Objective</h3><div>Both distal and proximal compression of the median nerve can lead to hand numbness inpatients.This study aimed to dissect the electrophysiological characteristics of median nerve motor fibers inpatients with carpal tunnel syndrome (CTS) caused by distal median nerve compression, C8 - T1 nerve root - type cervical spondylosis (CRS) resulting from proximal median nerve compression, and double - compression syndrome (DCS) with CTS combined with C8 - T1 CRS, so as to verify the double - nerve compression theory, provide evidence-based basis for clinical treatment.</div></div><div><h3>Methods</h3><div>Retrospective analysis of 30 subjects per group (CTS, CRS, DCS, controls) from a neurophysiological database. Parameters included distal motor latency (DML1-wrist, DML2-elbow), compound muscle action potential (CMAP1, CMAP2), wrist-elbow motor conduction velocity (MCV), and F-wave latency.</div></div><div><h3>Results</h3><div>For each indicator, both the CTS group and the DCS group showed statistically significant differences from the normal group. When comparing between the CTS group and the DCS group, there were no statistically significant differences in all data indicators. When comparing the CRS group with the normal group, there were no statistically significant differences in DML1 and DML2, but there were statistically significant differences in the shortest latency of the F wave and the motor conduction velocity (MCV).</div></div><div><h3>Conclusion</h3><div>Double compression does not cause more severe damage to the nerve compared with simple distal compression. Distal compression of the median nerve causes more severe damage to the peripheral nerve than proximal compression. For patients with double compression syndrome, clinical treatment should prioritize the treatment of the distal compression site.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 205-209"},"PeriodicalIF":2.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uptakes of boronophenylalanine in the in vitro and in situ glioblastoma and the potential efficacy of boron neutron capture therapy","authors":"Shining Zhang ,&nbsp;Yujie Wei ,&nbsp;Wenjiao Gu ,&nbsp;Shuangyi Li ,&nbsp;Ting Liu ,&nbsp;Limei Shuai ,&nbsp;Yu Tang ,&nbsp;Ying Jiang ,&nbsp;Xiaochun Zhou ,&nbsp;Yucai Wei ,&nbsp;Guan Wang ,&nbsp;Long Gu ,&nbsp;Yumin Li ,&nbsp;Futian Tang ,&nbsp;Daiying Zuo","doi":"10.1016/j.ibneur.2025.06.014","DOIUrl":"10.1016/j.ibneur.2025.06.014","url":null,"abstract":"<div><div>Human glioblastoma (GBM) is the most malignant brain tumor. Boron neutron capture therapy (BNCT) is proved to be a new technology for the effective treatment of GBM. We previously reported that boronophenylalanine (BPA), the boron drug used in BNCT prefers to the tumor of a mouse subcutaneous tumor model injected U87 and GL261, two GBM cell lines. The present study was designed to investigate the distribution of BPA in the tumor of a mouse <em>in situ</em> brain tumor model injected U87 and GL261 in the brain. The tumor model was evaluated by using small animal magnetic resonance imaging (MRI) and confirmed with the morphological observations. The boron concentration indicative of BPA in cells and tumor was measured using inductively coupled plasma atomic emission spectroscopy (ICP-AES). The results revealed that the protein of L-type amino acid transporter (LAT1) was highly expressed in both U87 and GL261 cells. In addition, boron concentration in U87 and GL261 cells was increased 1, 2, 3, 5 and 7 h after addition of BPA in a time-dependently manner. However, the boron concentration in the cells was rapidly decreased when the BPA in the medium was withdrawn for 1 h at each time point, reaching almost the same levels between the time points. In one mouse <em>in situ</em> brain tumor model injected U87 cells, the concentration of boron in the tumor tissue (25.529 μg/g) was higher than that in brain tissue (8.973 μg/g), blood (11.407 μg/g), heart (13.131 μg/g), liver (11.271 μg/g), spleen (15.631 μg/g) and lung (16.226 μg/g) respectively, having ratios of tumor/normal tissue 2.845, 2.238, 1.944, 2.265, 1.633 and 1.537 respectively. Similarly, in another mouse <em>in situ</em> brain tumor model injected GL261 cells, the concentration of boron in the tumor tissue (23.039 μg/g) was higher than that in brain tissue (8.141 μg/g), blood (9.573 μg/g), heart (12.119 μg/g), liver (9.609 μg/g), spleen (15.852 μg/g) and lung (12.565 μg/g) respectively, having ratios of tumor/normal tissue 2.831, 2.407, 1.901, 2.398, 1.453 and 1.834 respectively. The results suggest that uptakes of BPA in two cell lines occur in a time-dependent manner and that BPA preferred to distribute in brain tumor tissue than other normal tissues, having potential efficacy of BNCT.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"19 ","pages":"Pages 192-197"},"PeriodicalIF":2.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144470939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}