Horticulture Research最新文献_第7页

SlH3 and SlH4 promote multicellular Trichome formation and elongation by upregulating woolly in tomato

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-24 DOI: 10.1093/hr/uhaf008

Seong-Min Kim, Da-Min Choi, Jae-In Chun, Seong-Yeop Kim, Seong-Hyeon Kim, Jeong-Il Kim, Ji-in Jang, Keunhwa Kim, Soon Ju Park, Jang-Kyun Seo, Choonkyun Jung, Jin-Ho Kang

{"title":"SlH3 and SlH4 promote multicellular Trichome formation and elongation by upregulating woolly in tomato","authors":"Seong-Min Kim, Da-Min Choi, Jae-In Chun, Seong-Yeop Kim, Seong-Hyeon Kim, Jeong-Il Kim, Ji-in Jang, Keunhwa Kim, Soon Ju Park, Jang-Kyun Seo, Choonkyun Jung, Jin-Ho Kang","doi":"10.1093/hr/uhaf008","DOIUrl":"https://doi.org/10.1093/hr/uhaf008","url":null,"abstract":"Trichomes are tiny outgrowths on the plant epidermis that serve defensive purposes against various stresses. While the regulatory mechanisms underlying unicellular trichome development are well understood, those governing multicellular trichome formation remain largely unexplored. In this study, we reveal a new regulatory pathway involving the Hair3 (H3) and H4 genes, which encode C2H2 zinc finger proteins that participate in multicellular trichome development in tomato (Solanum lycopersicum). Using CRISPR-Cas9 to generate single- and double-knockout lines, we found that h3 and h4 single-mutant plants did not show altered trichome characteristics compared to wild-type plants. However, h3/h4 double-knockout plants displayed decreased densities of types I, VI, and VII trichomes, increased densities of types III and V trichomes, and reduced leaf and stem lengths of type I trichomes, revealing that H3 and H4 redundantly regulate trichome development. Notably, protein interaction assays demonstrated that H3 and H4 formed both homo- and hetero-dimers, supporting their cooperative role. Transcriptome and gene expression analyses identified H3 and H4 as key regulators of several genes involved in trichome development, including Woolly (Wo) and its downstream targets, such as Wox3b, MX1, H, and HD8. Protein-promoter assays showed that H3 and H4 did not directly bind to the Wo promoter but rather interacted with Wo, thereby enhancing the expression of Wo and Wox3b. These findings establish H3 and H4 as key regulators of trichome development and provide novel insights into the mechanisms controlling multicellular trichome development in tomato plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"16 4 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyotype variation patterns and phenotypic responses of hybrid progenies of triploid loquat (Eriobotrya japonica) provide new insight into aneuploid germplasm innovation 三倍体枇杷（Eriobotrya japonica）杂交后代的核型变异模式和表型响应为非整倍体种质创新提供了新的思路

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-22 DOI: 10.1093/hr/uhaf023

Peng Wang, Shangjian Yang, Meiyi Chen, Yingjia Liu, Qiao He, Haiyan Sun, Di Wu, Suqiong Xiang, Danlong Jing, Shuming Wang, Qigao Guo, Jiangbo Dang, Guolu Liang

{"title":"Karyotype variation patterns and phenotypic responses of hybrid progenies of triploid loquat (Eriobotrya japonica) provide new insight into aneuploid germplasm innovation","authors":"Peng Wang, Shangjian Yang, Meiyi Chen, Yingjia Liu, Qiao He, Haiyan Sun, Di Wu, Suqiong Xiang, Danlong Jing, Shuming Wang, Qigao Guo, Jiangbo Dang, Guolu Liang","doi":"10.1093/hr/uhaf023","DOIUrl":"https://doi.org/10.1093/hr/uhaf023","url":null,"abstract":"The sexual reproduction of triploids induces chromosomal karyotype variations, which are significant for germplasm resource innovation. Most triploid plants are with low fertility. Therefore, triploid offspring karyotypes’ variation pattern and phenotypic response remain poorly understood. Here, we employed three diploids with diverse genetic distances as male parents to cross-pollinate the female fertile triploid loquat Q24 to construct three experimental populations. The chromosome numbers of 93.82% of hybrid plants were 34~46 in three hybrid populations. All 168 aneuploids with 160 karyotypes and a small percentage of euploids were detected among 178 hybrids by the improved molecular karyotype analysis method. Further analysis revealed that when being transmitted to offspring, chromosome 5 of Q24 as disomy was with the highest frequency (&gt; 50%), while chromosome 12 was with the lowest frequency (≤ 30%). The frequency of Q24’s chromosomes being transmitted to offspring as disomy was influenced by the gene function on the chromosomes and the number of inter-chromosome collinear gene links. Whole genome resequencing showed that the Q24 alleles exhibited segregation distortions in the offspring aneuploid population. Transgenic experiments demonstrated that the EjRUN1 gene, which was on one segregation distortion region of Q24, promoted the seed viability of triploid Arabidopsis. Furthermore, chromosome number, dosage, and male parent genotype affected the aneuploid phenotype. These findings advance the understanding of genome genetic characteristics of triploid loquat, and provide a reference for germplasm innovation of loquat rapidly through triploid sexual reproduction.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"104 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RcSRR1 interferes with the RcCSN5B-mediated deneddylation of RcCRL4 to modulate RcCO proteolysis and prevent rose flowering under red light RcSRR1干扰rccsn5b介导的rcrl4去醛化，从而调节RcCO蛋白水解，阻止玫瑰在红光下开花

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-21 DOI: 10.1093/hr/uhaf025

Weinan Wang, Jingjing Sun, Chunguo Fan, Guozhen Yuan, Rui Zhou, Jun Lu, Jinyi Liu, Changquan Wang

{"title":"RcSRR1 interferes with the RcCSN5B-mediated deneddylation of RcCRL4 to modulate RcCO proteolysis and prevent rose flowering under red light","authors":"Weinan Wang, Jingjing Sun, Chunguo Fan, Guozhen Yuan, Rui Zhou, Jun Lu, Jinyi Liu, Changquan Wang","doi":"10.1093/hr/uhaf025","DOIUrl":"https://doi.org/10.1093/hr/uhaf025","url":null,"abstract":"Light is essential for rose (Rosa spp.) growth and development. Different light qualities play differing roles in the rose floral transition, but the molecular mechanisms underlying their effects are not fully understood. Here we observed that red light suppresses rose flowering and increases the expression of Sensitivity to Red Light Reduced 1 (RcSRR1) compared with white light. Virus-induced gene silencing (VIGS) of RcSRR1 led to early flowering under white light and especially under red light, suggesting that this gene is a flowering repressor with a predominant function under red light. We determined that RcSRR1 interacts with the COP9 Signalosome Subunit 5B (RcCSN5B), while RcCSN5B, RcCOP1 and RcCO physically interact with each other. Furthermore, the RcCSN5B-induced deneddylation of Cullin4-RING E3 Ubiquitin Ligase (RcCRL4) in rose was reduced by the addition of RcSRR1, suggesting that the interaction between RcSRR1 and RcCSN5B relieves the deneddylation of the RcCRL4-COP1/SPA complex to enhance RcCO proteolysis, which subsequently suppresses the transcriptional activation of RcFT and ultimately flowering. Far-red Light Related Sequence Like 1 (RcFRSL3) was shown to specifically bind to the G-box motif of the RcSRR1 promoter to repress its transcription, removing its inhibition of RcFT expression and inducing flowering. Red light inhibited RcFRSL3 expression, thereby promoting the expression of RcSRR1 to inhibit flowering. Taken together, these results provide a previously uncharacterized mechanism by which the RcFRSL3–RcSRR1–RcCSN5B module targets RcCO stability to regulate flowering under different light conditions in rose plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"74 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome assembly of pomegranate highlights structural variations driving population differentiation and key loci underpinning cold adaption 石榴基因组组装揭示了驱动群体分化的结构变异和支撑冷适应的关键位点

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-21 DOI: 10.1093/hr/uhaf022

Xiang Luo, Zhenyang Shua, Diguang Zhao, Beibei Liu, Hua Luo, Ying Chen, Dong Meng, Zhihua Song, Qing Yang, Zicheng Wang, Dong Tang, Xingguo Zhang, Juan Zhang, Kai Ma, Wen Yao

{"title":"Genome assembly of pomegranate highlights structural variations driving population differentiation and key loci underpinning cold adaption","authors":"Xiang Luo, Zhenyang Shua, Diguang Zhao, Beibei Liu, Hua Luo, Ying Chen, Dong Meng, Zhihua Song, Qing Yang, Zicheng Wang, Dong Tang, Xingguo Zhang, Juan Zhang, Kai Ma, Wen Yao","doi":"10.1093/hr/uhaf022","DOIUrl":"https://doi.org/10.1093/hr/uhaf022","url":null,"abstract":"Cold damage poses a significant challenge to the cultivation of soft-seeded pomegranate varieties, hindering the growth of the pomegranate industry. The genetic basis of cold tolerance in pomegranates has remained elusive, largely due to the lack of high-quality genome assemblies for cold-tolerant varieties and comprehensive population-scale genomic studies. In this study, we addressed these challenges by assembling a high-quality chromosome-level reference genome for 'Sanbai', a pomegranate variety renowned for its freezing resistance, achieving an impressive contig N50 of 15.93 Mb. This robust assembly, enhanced by long-read sequencing of 38 pomegranate accessions, facilitated the identification of 14,239 polymorphic structural variants, revealing their critical roles in genomic diversity and population differentiation related to cold tolerance. Of particular significance was the discovery of a ~5.4-Mb inversion on chromosome 1, which emerged as an important factor affecting cold tolerance in pomegranate. Moreover, through the integration of bulked segregant analysis, differential selection analysis, and genetic transformation techniques, we identified and validated the interaction between the PgNAC12 transcription factor and PgCBF1, disclosing their pivotal roles in response to cold stress. These findings mark a significant advancement in pomegranate genomics, offering novel insights into the genetic mechanisms of cold tolerance and providing valuable resources for the genetic improvement of soft-seeded pomegranate varieties.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"14 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PbDELLA-PbMYB56-PbCYP78A6 Module Regulates GA4+7-Induced Pseudo-Embryo Development and Parthenocarpy in Pear (Pyrus bretschneideri) pbdella - pbmyb56 - pbbcyp78a6模块调控GA4+7诱导的梨伪胚发育和孤雌实

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-21 DOI: 10.1093/hr/uhaf021

Haiqi Zhang, Jingjing Cheng, Xue Wang, Pingyuan Dai, Hongjuan Zhang, Fengli Zhou, Chengquan Yang, Rui Zhai, Zhigang Wang, Lingfei Xu

{"title":"The PbDELLA-PbMYB56-PbCYP78A6 Module Regulates GA4+7-Induced Pseudo-Embryo Development and Parthenocarpy in Pear (Pyrus bretschneideri)","authors":"Haiqi Zhang, Jingjing Cheng, Xue Wang, Pingyuan Dai, Hongjuan Zhang, Fengli Zhou, Chengquan Yang, Rui Zhai, Zhigang Wang, Lingfei Xu","doi":"10.1093/hr/uhaf021","DOIUrl":"https://doi.org/10.1093/hr/uhaf021","url":null,"abstract":"Parthenocarpy can ensure fruit setting without fertilization and generate seedless fruits. PbCYP78A6 has been shown to play a role in gibberellin (GA)-induced parthenocarpy in pears. However, the transcriptional response mechanism of PbCYP78A6 to GA remains unclear. In this study, using a yeast one-hybrid assay combined with co-expression analysis, PbMYB56 was initially identified as a transcription regulator of PbCYP78A6, which was further confirmed by electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assays. The biofunction of PbMYB56 was further verified using transient transgene tests, stable transgenic pear callus and tomato. PbMYB56 overexpression resulted in reduced cell death and higher fluorescence intensity after fluoresce diacetate (FDA) staining, as well as delayed fruit-drop by increasing PbCYP78A6 expression in un-pollinated pear fruitlets and callus. In contrast, silencing PbMYB56 caused cell death and early fruit-drop with decreased PbCYP78A6 expression. Moreover, after emasculation, heterologous overexpression of PbMYB56 induced parthenocarpy and enlarged seed size in pollinated tomato fruits. Silencing SlMYB56, a homolog of PbMYB56 in tomatoes, resulted in smaller fruit and seed size, and these traits were restored by co-overexpression with PbCYP78A6. Furthermore, we investigated the protein interaction between PbMYB56 and PbDELLA, which is crucial component of the GA signaling pathway. This interaction inhibited PbMYB56-induced transcriptional activation of PbCYP78A6. Co-overexpression of PbMYB56 and PbDELLA contributed to reduced seed development and loss of parthenocarpy potential in tomatoes. Collectively, our study identifies PbDELLA-PbMYB56-PbCYP78A6 as a regulatory module of GA4+7-induced pseudo-embryo and parthenocarpy development, offering insights into the mechanism underlying parthenocarpy formation in pears.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"22 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin suppresses ethylene biosynthesis by inhibiting transcription factor MdREM10 during apple fruit ripening 褪黑素通过抑制苹果果实成熟过程中转录因子MdREM10抑制乙烯生物合成

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-21 DOI: 10.1093/hr/uhaf020

Chen Li, Qian Yu, Yajing Si, Yuling Liang, Shijiao Lin, Guangxin Yang, Weiting Liu, Yinglin Ji, Aide Wang

{"title":"Melatonin suppresses ethylene biosynthesis by inhibiting transcription factor MdREM10 during apple fruit ripening","authors":"Chen Li, Qian Yu, Yajing Si, Yuling Liang, Shijiao Lin, Guangxin Yang, Weiting Liu, Yinglin Ji, Aide Wang","doi":"10.1093/hr/uhaf020","DOIUrl":"https://doi.org/10.1093/hr/uhaf020","url":null,"abstract":"Ethylene, a plant hormone, is essential for apple (Malus domestica) ripening. The precise molecular mechanism by which melatonin (MT) influences ethylene biosynthesis during apple fruit ripening remains unclear. This study found that exogenous MT treatment inhibited ethylene production and postponed apple fruit ripening. The endogenous MT content of apple fruits exhibited an inverse correlation with ethylene production during fruit ripening, suggesting that MT functions as a ripening suppressor in apple fruits. MT treatment suppressed the expression of key ethylene biosynthesis genes, MdACS1 and MdACO1, during apple fruit ripening. MT treatment decreased the expression levels of transcription factors MdREM10 and MdZF32. MdREM10 binds to the MdERF3 promoter, enhancing its expression and subsequently promoting MdACS1 transcription. Furthermore, MdREM10 directly bound to the MdZF32 promoter, promoting its transcription. MdZF32 directly bound to the MdACO1 promoter, inducing its expression. The findings suggested that MT suppresses ethylene biosynthesis and fruit ripening by inhibiting MdREM10, which indirectly promotes MdACS1 transcription via MdERF3 upregulation, and MdACO1 transcription via MdZF32 upregulation.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"25 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of morphogenesis and metabolism by plant cell biomechanics: From model plants to traditional herbs 植物细胞生物力学对形态发生和代谢的调节：从模式植物到传统草药

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-16 DOI: 10.1093/hr/uhaf011

Zhengpeng Wang, Xiaoming Ye, Luqi Huang, Yuan Yuan

引用次数: 0

PhWRKY30 activates salicylic acid biosynthesis to positively regulate antiviral defense response in petunia PhWRKY30激活水杨酸生物合成，积极调节矮牵牛花抗病毒防御反应

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-15 DOI: 10.1093/hr/uhaf013

Meiling Wang, Yanping Yuan, Yike Zhao, Zhuo Hu, Shasha Zhang, Jianrang Luo, Cai-Zhong Jiang, Yanlong Zhang, Daoyang Sun

{"title":"PhWRKY30 activates salicylic acid biosynthesis to positively regulate antiviral defense response in petunia","authors":"Meiling Wang, Yanping Yuan, Yike Zhao, Zhuo Hu, Shasha Zhang, Jianrang Luo, Cai-Zhong Jiang, Yanlong Zhang, Daoyang Sun","doi":"10.1093/hr/uhaf013","DOIUrl":"https://doi.org/10.1093/hr/uhaf013","url":null,"abstract":"Petunia (Petunia hybrida) plants are highly threatened by a diversity of viruses, causing substantial damage to ornamental quality and seed yield. However, the regulatory mechanism of virus resistance in petunia is largely unknown. Here, we revealed that a member of petunia WRKY transcription factors, PhWRKY30, was dramatically up-regulated following Tobacco rattle virus (TRV) infection. Down-regulation of PhWRKY30 through TRV-based virus-induced gene silencing increased green fluorescent protein (GFP)-marked TRV RNA accumulation and exacerbated the symptomatic severity. In comparison to wild-type (WT) plants, PhWRKY30-RNAi transgenic petunia plants exhibited a compromised resistance to TRV infection, whereas an enhanced resistance was observed in PhWRKY30-overexpressing (OE) transgenic plants. PhWRKY30 affected salicylic acid (SA) production and expression of arogenate dehydratase 1 (PhADT1), phenylalanine ammonia-lyase 1 (PhPAL1), PhPAL2b, non-expressor of pathogenesis-related proteins 1 (PhNPR1), and PhPR1 in SA biosynthesis and signaling pathway. SA treatment restored the reduced TRV resistance to WT levels in PhWRKY30-RNAi plants, and application of SA biosynthesis inhibitor 2-aminoindan-2-phosphonic acid inhibited promoted resistance in PhWRKY30-OE plants. The protein-DNA binding assays showed that PhWRKY30 specifically bound to the promoter of PhPAL2b. RNAi silencing and overexpression of PhPAL2b led to decreased and increased TRV resistance, respectively. The transcription of a number of reactive oxygen species- and RNA silencing-associated genes was changed in PhWRKY30 and PhPAL2b transgenic lines. PhWRKY30 and PhPAL2b were further characterized to be involved in the resistance to Tobacco mosaic virus (TMV) invasion. Our findings demonstrate that PhWRKY30 positively regulates antiviral defense against TRV and TMV infections by modulating SA content.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"3 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Vesicle-Mediated Regulation of Pollen Tube Growth through BFA Inhibition and AS-ODN Targeting of TfRABA4D in Torenia fournieri 通过BFA抑制和AS-ODN靶向TfRABA4D研究花粉管生长的囊泡介导调控

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-15 DOI: 10.1093/hr/uhaf018

Xingyue Jin, Akane G Mizukami, Satohiro Okuda, Tetsuya Higashiyama

{"title":"Investigating Vesicle-Mediated Regulation of Pollen Tube Growth through BFA Inhibition and AS-ODN Targeting of TfRABA4D in Torenia fournieri","authors":"Xingyue Jin, Akane G Mizukami, Satohiro Okuda, Tetsuya Higashiyama","doi":"10.1093/hr/uhaf018","DOIUrl":"https://doi.org/10.1093/hr/uhaf018","url":null,"abstract":"In flowering plants, pollen tube growth is essential for delivering immotile sperm cells during double fertilization, directly influencing seed yield. This process relies on vesicle-mediated trafficking to drive tip growth and maintain fertility. However, investigating pollen tube growth is challenging in non-model plants due to the lack of transgenic tools. Here, we established a method to transiently inhibit vesicle activity in pollen tubes of the wishbone flower (Torenia fournieri), a classic plant for sexual reproduction studies, using brefeldin A (BFA) and antisense oligodeoxynucleotides (AS-ODNs) targeting key genes. BFA broadly disrupted vesicle gradient homeostasis in T. fournieri pollen tubes, leading to widespread changes in cell wall deposition, ROS distribution, and pollen tube morphology. To assess the role of specific genes, we designed AS-ODNs against TfANX, the sole ANXUR homolog in T. fournieri, which successfully penetrated cell membranes and suppressed TfANX expression. This inhibition impaired pollen tube tip growth, causing pollen tube leakage at the shank region and, in some cases, multiple leakages. Similarly, AS-ODN targeting TfRABA4D, a pollen-specific vesicle regulator, induced a bulging phenotype and disrupted pectin deposition and reduced ROS distribution, mirroring BFA effects. These findings elucidate vesicle-mediated regulation in pollen tube tip growth and introduce an accessible method for genetic manipulation in reproductive research of non-model plants.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"68 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the tomato co-chaperone gene Sldnaj harboring a promoter deletion with susceptibility to Tomato spotted wilt virus (TSWV) 启动子缺失的番茄协同伴侣基因Sldnaj与番茄斑点枯萎病毒易感性的关联

IF 8.7 1区农林科学

Horticulture Research Pub Date : 2025-01-15 DOI: 10.1093/hr/uhaf019

Shiming Qi, Liangzhe Meng, Qianqi Lou, Yushun Li, Yuanbo Shen, Shijie Zhang, Xinyu Wang, Pan Zhao, Jin Wang, Bo Wang, Xiubin Chen, Chunmei Zhang, Yu Du, Jiantao Zhao, Xiangqiang Zhan, Yan Liang

{"title":"Association of the tomato co-chaperone gene Sldnaj harboring a promoter deletion with susceptibility to Tomato spotted wilt virus (TSWV)","authors":"Shiming Qi, Liangzhe Meng, Qianqi Lou, Yushun Li, Yuanbo Shen, Shijie Zhang, Xinyu Wang, Pan Zhao, Jin Wang, Bo Wang, Xiubin Chen, Chunmei Zhang, Yu Du, Jiantao Zhao, Xiangqiang Zhan, Yan Liang","doi":"10.1093/hr/uhaf019","DOIUrl":"https://doi.org/10.1093/hr/uhaf019","url":null,"abstract":"Tomato spotted wilt virus (TSWV) poses a significant threat as a devastating pathogen to the global production and quality of tomato (Solanum lycopersicum). Mining novel resistance genes within the tomato germplasm is an effective and environmentally friendly approach to combat TSWV. In this study, we investigated the mechanisms underlying high TSWV resistance in a specific tomato line after experimental inoculation, despite not possessing any known TSWV resistance genes. The candidate causal genes of disease resistance traits were finely mapped by constructing different genetic populations and performing bulk segregant analysis sequencing. This approach identified SlDnaJ (Solyc10g081220) as a key locus potentially regulating TSWV resistance. We determined a structural variant of SlDnaJ (designated Sldnaj) containing a 61-bp promoter sequence deletion that was specifically present in the germplasm of the susceptible M82 tomato plant lines. Sldnaj-knockout transgenic plants were significantly more resistant to TSWV than wild-type plants. Up-regulated expression of Sldnaj affected the salicylic acid/jasmonic acid signaling pathway, which induced and promoted the systemic infection of TSWV in M82 susceptible plants. In summary, this study identified a new candidate TSWV susceptibility gene with a natural deletion variation in tomato. These findings provide insights into the molecular mechanism underlying pathogen resistance while offering a target for breeding strategies of tomato with TSWV resistance.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"37 1","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0