Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao最新文献_第10页

[A study on the relationship between respiratory tract viruses antigen in peripheral blood mononuclear cells and their antibody from children with steroid-sensitive and simple nephrotic syndrome]. [类固醇敏感型单纯性肾病综合征患儿外周血单个核细胞呼吸道病毒抗原与其抗体关系的研究]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

Z Wang, L Mu, Y Tao, L Yuan

{"title":"[A study on the relationship between respiratory tract viruses antigen in peripheral blood mononuclear cells and their antibody from children with steroid-sensitive and simple nephrotic syndrome].","authors":"Z Wang, L Mu, Y Tao, L Yuan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore whether respiratory tract viruses infection was involved in the pathogenesis of sterioid-senstive and simple nephrotic syndrome(SSSNS).Methods: Using APAAP and ELISA, the authors assayed the expression of respiratory tract viruses antigen in peripheral blood mononuclear cells (PBMC) and the production of viruses antibody in serum from 20 cases of children with steroid-sensitive and simple nephrotic syndrome.Results: The positive rate of viruses in the active stage group of SSSNS children increased (P < 0.05), compared with that in the remission group and the healthy control group. The positive rate of virus detection in SSSNS children was closely related with the quantity of urinary protein(P < 0.05). The positive rate of virus detection in the group with urinary protein > or = 50 mg/(kg.d) was higher than that in the group with urinary protein < 50 mg/(kg.d).Conclusion: The onset of SSSNS may be related to the infection of respiratory tract viruses.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"559-61"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22197083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Study on psychoprophylaxis and monoamines neurotransmitter of patients with burning mouth syndrome]. [灼口综合征患者精神预防及单胺类神经递质研究]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

M Lin, B Li, F Gu, Y Yue, Y Huang, Q Chen, G Zeng, J Xia

{"title":"[Study on psychoprophylaxis and monoamines neurotransmitter of patients with burning mouth syndrome].","authors":"M Lin, B Li, F Gu, Y Yue, Y Huang, Q Chen, G Zeng, J Xia","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Burning mouth syndrome (BMS) is a chronic ache disease, usually occurring in middle aged and old women. This study sought to understand the psychopathologic aspect and monoamines neurotransmitters in the plasma of the patients with BMS.Methods: Thirty cases were selected (26 females, 4 males); 30 normal control subjects were similar to the BMS cases on age and sex. All subjects were required to complete the Eysenck personality questionnaire (EPQ), and the Self-report Symptom Inventory, Symptom Check List-90 (SCL-90) questionnaire. In case a subject's L (lie) score exceeded 50, she (he) would be removed from the test. 2 ml of blood was drawn from the subject under restine conditions with a fast in the morning to examine norepinephrine and epinephrine contents by high efficient liquid chromatography. Chi-square test, analysis of variance and t'-test were performed.Results: The BMS group had higher scores of nervousness (N) and poikilergasia (P) and lower score of extro/introversion (E) as compared with the control (P < 0.05). The personality types in BMS group were focused on introversion and instability, but in the control group the types were focused on extroversion and stability (P < 0.05). The scores of 9 emotional factors of BMS group were significantly higher than those of the control group (P < 0.05), which indicated that the BMS patients had suffered from serial psychic disorders. The level of plasma norepinephrine in the BMS patients was higher than that of the control (P < 0.01).Conclusion: The personality of BMS patients raised body response to harmful stimulations, and obvious psychic disorders in the patient may cause the functional disorders in central and sympathetic nervous systems, which may be associated with BMS' occurrence.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"576-8"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22197088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Human toll like receptor gene expression in human pulmonary granule epithelial cells and umbilical vein endothelial cells]. [toll样受体基因在人肺颗粒上皮细胞和脐静脉内皮细胞中的表达]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

F Liang, N Huang, B Wang

引用次数: 0

[The studies on microtensile bond strength measurements of two dentin adhesives]. [两种牙本质粘接剂微拉伸粘结强度测量的研究]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

B Xie, S H Dickens, G E Schumacher, A A Giuseppetti

{"title":"[The studies on microtensile bond strength measurements of two dentin adhesives].","authors":"B Xie, S H Dickens, G E Schumacher, A A Giuseppetti","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The purpose of this study was to investigate the feasibility of a modified microtensile method used to test bond strengths of two current one-bottle dentin bond systems (Prime & Bond NT, PBNT; Prime one Mirage, P-One) with a parallel match design.Methods: 15 extracted, caries-free human molars were cut to expose occlusal dentin. A 5 mm deep slot was prepared in each crown to divide the crown into nearly equal halves for accepting treatment of the two dentin bond systems, respectively. After 24 h storage in distilled water at 37 degrees C, the bonded teeth were subjected to two treatments: 5 teeth were tested without further treatment and 10 teeth were thermocycled (2400 cycles, between 5 degrees C and 55 degrees C) prior to bond strength testing. Hour-glass shaped specimens with a distance of approximately 1.0 mm at the narrowest portion were cut from each tooth and tested in tensile mode.Results: Bond strengths (mean MPa) were: for PBNT: 42 & 31, and for P-One 64 & 38 without and with thermocycling, respectively. Two-way ANOVA revealed a significant difference in bond strengths(P < 0.001) between the two systems and when thermocycled. However, a pairwise multiple comparison (Tukey test) showed that after thermocycling the difference between the two systems was not significant (P > 0.05). Regression analysis showed that a correlation existed between the two systems' tensile bond strength values grouped by tooth (correlation coefficient r = 0.575, P < 0.05).Conclusion: The modified microtensile method with a parallel match design is feasible and suitable for evaluating two different bonding systems or dentin treatments.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"519-23"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22196086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The binding characteristics of insulin-MTX to insulin receptor]. [胰岛素- mtx与胰岛素受体的结合特性]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

X Ou, A Kuang, Z Liang, X Peng, Y Zhong

{"title":"[The binding characteristics of insulin-MTX to insulin receptor].","authors":"X Ou, A Kuang, Z Liang, X Peng, Y Zhong","doi":"","DOIUrl":"","url":null,"abstract":"Objective: It has been reported that several kinds of tumors express increased insulin receptor and the molecules of insulin can be internalized in cells and may thence enter into the nuclei mediated by insulin receptor. In this study, we investigated the receptor binding characteristics of insulin-MTX for the possibility of using insulin as a carrier for carcinoma targeted therapy by receptor mediation.Methods: MTX(methotrexate) was covalently linked to insulin directly. The insulin-MTX conjugate was purified by polyacrylamine agarose gel electrophoresis and analysed by high performance liquid chromatography and SDS- polyacrylamine agarose gel electrophoresis. Histologically confirmed human hepatocellular carcinoma specimens were obtained from patients at surgery and immediately frozen under -80 degrees C. Cell membrane fractions were isolated by sucrose density gradient centrifugation. Competitive displacement of 125I-insulin with insulin and insulin-MTX binding to insulin receptor were carried out and the values of IC50 and Ki were calculated so as to observe the characteristics of insulin-MTX binding to insulin receptor.Results: Insulin-MTX competed as effectively as insulin with 125I-insulin for insulin receptor. The values of IC50 and Ki for insulin-MTX were 93.82 +/- 19.32 nmol/L and 91.88 +/- 16.86 nmol/L respectively, while the values of IC50 and Ki for insulin were 5.01 +/- 1.24 nmol/L and 4.85 +/- 1.12 nmol/L respectively.Conclusion: Insulin-MTX could bind with insulin receptor with high affinity. The result demonstrates us that there is a possibility of using insulin as a carrier for carcinoma targeted therapy by receptor mediation.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"538-40"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22196571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The expression of tyrosine-kinase receptor flk-1 and hypoxic pulmonary vascular remodeling in the lung of rats exposed to hypoxia]. [缺氧大鼠肺组织中酪氨酸激酶受体flk-1的表达及缺氧肺血管重构]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

D Cheng, X Xiao, W Chen

{"title":"[The expression of tyrosine-kinase receptor flk-1 and hypoxic pulmonary vascular remodeling in the lung of rats exposed to hypoxia].","authors":"D Cheng, X Xiao, W Chen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To assess the role of tyrosine-kinase receptor flk-1 in the development of hypoxic pulmonary vascular remodeling.Methods: We divided 20 male Wistar rats into two groups(control vs hypoxia) and exposed them to normoxic condition and isobaric hypoxia for 3 weeks respectively. The pulmonary artery pressure was measured by right cardiac catheterization. The expression of flk-1 in lung tissues was measured by immunohistochemical staining. Histologic sections of the lungs were examined by a computerized image analyser.Results: In hypoxic rats, the pulmonary artery pressure was significantly raised to a higher level, P < 0.01; the cell number of vascular wall was significantly increased. The results also demonstrated that chronic hypoxia brought about the significant increment in thickness of wall with narrowing of lumen of pulmonary arterioles, and the increment in the percent of vascular wall thickness/vascular external diameter (WT%) and the percent of vascular wall area/total vascular area (WA%), P < 0.01. The positive staining of flk-1 in the wall of pulmonary arteriole of rats treated with hypoxia was significantly stronger than that of normal rats, P < 0.01. Positive correlations were seen between the increment of expression of flk-1 with WT% and WA% (r = 0.714, 0.738, P < 0.01).Conclusion: Chronic hypoxia can induce an increasing expression of flk-1, and the flk-1 may play an important role in the pathogenesis of hypoxic pulmonary vascular remodeling.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"489-91"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22195044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Study on the content of serum HDL subclasses in obese subjects]. 肥胖者血清HDL亚类含量的研究

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

Y Xu, M Fu, Y Xu, B Liu

{"title":"[Study on the content of serum HDL subclasses in obese subjects].","authors":"Y Xu, M Fu, Y Xu, B Liu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the change of the contents of serum HDL subclasses in obese subjects.Methods: Contents of serum HDL subclasses in healthy controls(n = 64) and obese subjects(n = 58) were determined by two-dimensional gel electrophoresis associated with immunodetection method.Results: The pre-beta 1 HDL, HDL3c and HDL3b contents increased significantly (P < 0.001) and the HDL2b content decreased significantly (P < 0.001) in obese subjects as compared with those in healthy controls. In male obese subjects, the pre-beta 1 HDL and HDL3c levels were significantly higher(P < 0.01 and P < 0.05, respectively) and HDL2b level was significantly lower(P < 0.05), compared with those in female obese subjects. In male control subjects, the level of pre-beta 1 HDL was significantly higher than that in female subjects. In obese subjects, the concentrations of BMI and serum TG had positive correlation with those of pre-beta 1 HDL(r = 0.382, P < 0.001), HDL3c(r = 0.232, P < 0.05) and HDL3b(r = 0.294, P < 0.01) and negative correlation with that of HDL2b(r = -0.304, P < 0.01).Conclusion: The particle size of HDL in obese subjects showed a general shift toward smaller size, which indicated that the maturation of HDL could be abnormal in obese subjects. The particle size of HDL was found to be smaller in both obese and control male subjects than in female subjects, suggesting that sex seems to be related to the contents of serum HDL subclasses.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"509-12"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22196083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A study of bone development and metabolism in childhood]. [儿童骨骼发育和新陈代谢的研究]。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

M Liu, H Zheng, M Peng, L Liu, Z Peng, D Yang, J Shang, L Huang, L Suo

{"title":"[A study of bone development and metabolism in childhood].","authors":"M Liu, H Zheng, M Peng, L Liu, Z Peng, D Yang, J Shang, L Huang, L Suo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the regularity of bone development and metabolism in childhood.Methods: A survey was made to collect and analyze the data on bone biochemistry, the density of lumbar vertebrae and the X-ray examination of calcaneus in 224 healthy children aged 3-15 years in the urban area of Chengdu.Results: Comparative analysis of serum and urine calcium and phosphate concentrations showed no significant difference between the age groups. The serum alkaline phosphatase (ALP) and bone alkaline phosphatase (BALP) were found to increase with age and reach their peak values in children aged 7-12 years, and their values were significantly higher than that of adults (P < 0.05). The density of lumbar vertebrae and the trabecular bone of calcanesus also developed with age.Conclusion: The measurements of the bone biochemistry, bone density and the trabecular bone of calcaneus which reflect the bone development and metabolism have their special regularity in childhood. The criteria in use for adulthood is not fit for the evaluation in childhood. The data obtained can be used in clinical diagnosis and treatment of the bone metabolic diseases of children.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"529-31"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22196568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The preparation of norA gene probe in Staphylococcus aureus]. 金黄色葡萄球菌norA基因探针的制备

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

L Zhong, P Feng, X Fan, X Lü, B Lei

引用次数: 0

[Determination of roxithromycin in serum by high-performance liquid chromatography with ultraviolet detection]. 紫外检测高效液相色谱法测定血清中罗红霉素的含量。

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao Pub Date : 2001-12-01

Y Qin, Y Zou, M Liang, Y Huang, Q Yu, P Feng

{"title":"[Determination of roxithromycin in serum by high-performance liquid chromatography with ultraviolet detection].","authors":"Y Qin, Y Zou, M Liang, Y Huang, Q Yu, P Feng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To make better the RP-HPLC method for the determination of roxithromycin(RM) in human serum.Methods: RM and clarithromycin (internal standard) were extracted from alkalinized serum sample (500 microliters) with methylene chloride. After evaporation of the organic layer, the residue was dissolved in 100 microliters of acetonitrile-ammonium phosphate (1:1, pH 6.0) and washed with n-hexane, then 20 microliters was injected onto a column (5 microns, 15 cm x 4.6 mm) of Penomenex luna C18. The mobile phase was acetonitrile-0.05 mol/L phosphoric acid (39:19:42, adjusted to pH 7.2 with ammonia water) pumped at 1.2 ml/min through the column. The variable wavelength UV detector operated at 0.01 aufs and the wavelength was set at 210 nm.Results: The retention times for RM and clarithromycin were 4.4 min and 5.0 min, respectively. Standard curve was linear in the concentration range of 0.25 to 32 mg/L. The detection limit in serum was 0.06 mg/L; the average method recovery 97.4%; the inter-day RSD less than 3.0%.Conclusion: This method was found to be simple, rapid, sensitive and accurate for determination of RM in human serum.","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"612-4"},"PeriodicalIF":0.0,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22196958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0