{"title":"金黄色葡萄球菌norA基因探针的制备","authors":"L Zhong, P Feng, X Fan, X Lü, B Lei","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To prepare norA gene probe for studies on the mechanism of resistance to fluoroquinolones mediated by norA gene in S. aureus.</p><p><strong>Methods: </strong>The Dig-labeled norA gene probe was made by PCR.</p><p><strong>Results: </strong>This method is simple and feasible. We have got a lot of probes in a short time. The sensitivity of norA gene probe is high; the probe is secure, easy to use and can be stored for a long time.</p><p><strong>Conclusion: </strong>The probe is applicable to further studies on the mechanism of resistance to fluoroquinolones mediated by norA in S. aureus.</p>","PeriodicalId":13173,"journal":{"name":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","volume":"32 4","pages":"541-2, 584"},"PeriodicalIF":0.0000,"publicationDate":"2001-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The preparation of norA gene probe in Staphylococcus aureus].\",\"authors\":\"L Zhong, P Feng, X Fan, X Lü, B Lei\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To prepare norA gene probe for studies on the mechanism of resistance to fluoroquinolones mediated by norA gene in S. aureus.</p><p><strong>Methods: </strong>The Dig-labeled norA gene probe was made by PCR.</p><p><strong>Results: </strong>This method is simple and feasible. We have got a lot of probes in a short time. The sensitivity of norA gene probe is high; the probe is secure, easy to use and can be stored for a long time.</p><p><strong>Conclusion: </strong>The probe is applicable to further studies on the mechanism of resistance to fluoroquinolones mediated by norA in S. aureus.</p>\",\"PeriodicalId\":13173,\"journal\":{\"name\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"volume\":\"32 4\",\"pages\":\"541-2, 584\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[The preparation of norA gene probe in Staphylococcus aureus].
Objective: To prepare norA gene probe for studies on the mechanism of resistance to fluoroquinolones mediated by norA gene in S. aureus.
Methods: The Dig-labeled norA gene probe was made by PCR.
Results: This method is simple and feasible. We have got a lot of probes in a short time. The sensitivity of norA gene probe is high; the probe is secure, easy to use and can be stored for a long time.
Conclusion: The probe is applicable to further studies on the mechanism of resistance to fluoroquinolones mediated by norA in S. aureus.
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