He Zhao, Destinie Webster, Twyla Perace, Ahmed Mostafa
{"title":"Characterisation of the HLA-DPB1*1748:01 Allele by Next-Generation Sequencing","authors":"He Zhao, Destinie Webster, Twyla Perace, Ahmed Mostafa","doi":"10.1111/tan.70313","DOIUrl":"https://doi.org/10.1111/tan.70313","url":null,"abstract":"<div>\u0000 \u0000 <p>HLA-DPB1*1748:01 differs from HLA-DPB1*04:01:01:01 by one nucleotide substitution in codon-1 in exon 1.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiba Iraqi Møller, Camilla Moldrup Christiansen, Freja Rueholm Klok, Nanna Heldager Pedersen, Thomas Blauenfeldt, Katrine Folmann Finne, Henriette Svarre Nielsen, Thomas Vauvert F. Hviid
{"title":"Investigations of HLA-F and HLA-G 3′UTR Polymorphisms in Preeclampsia and Fetal Growth Restriction Indicate a Possible Role of HLA-F-HLA-G Haplotypes and Diplotypes","authors":"Hiba Iraqi Møller, Camilla Moldrup Christiansen, Freja Rueholm Klok, Nanna Heldager Pedersen, Thomas Blauenfeldt, Katrine Folmann Finne, Henriette Svarre Nielsen, Thomas Vauvert F. Hviid","doi":"10.1111/tan.70293","DOIUrl":"https://doi.org/10.1111/tan.70293","url":null,"abstract":"<p>HLA-F and HLA-G may be involved in the pathogeneses of preeclampsia and fetal growth restriction (FGR). However, the functions of HLA-F and HLA-G in placental dysfunction remain unclear. The aim was to investigate differences in the prevalence of specific HLA-F and HLA-G gene allelic polymorphisms, genotypes, haplotypes, and diplotypes between controls and cases with preeclampsia or FGR. In total, blood samples from 365 pregnant females (controls, <i>n</i> = 192; preeclampsia, <i>n</i> = 164; FGR, <i>n</i> = 19) in their second and third trimester, and corresponding cordial blood samples (reflecting newborns, <i>n</i> = 160) were obtained after delivery. Genomic DNA was sequenced with a focus on the specific gene polymorphisms in the HLA-F gene locus, especially the single nucleotide polymorphisms (SNPs) rs1362126 (G/A), rs2523405 (T/G) and rs2523393 (A/G), as well as the rs371194629 (14-bp ins/del) in the 3′UTR of HLA-G. Haplotype and diplotype distributions were obtained using PHASE v2.1, and linkage disequilibrium analyses were performed. SNPs in the HLA-F gene locus and the 3′UTR of HLA-G were not associated with the risk of preeclampsia or FGR. The SNPs did not correlate with fetal-placental weight ratio, deviation of birth weight at gestational age, and placental weight. However, a trend towards an absence of certain HLA-F-HLA-G extended diplotypes in preeclampsia was observed. The current study does not support associations of the investigated HLA-F SNPs with preeclampsia or FGR. However, further studies are needed to evaluate the possible role of certain fetal HLA-F-HLA-G extended haplotypes and diplotypes in preeclampsia.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tan.70293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Full Genomic Sequence of the HLA-C*01:289 Allele Identified by Next Generation Sequencing","authors":"John Jeongseok Yang, Sohyeon Eom, Eun-Suk Kang","doi":"10.1111/tan.70294","DOIUrl":"https://doi.org/10.1111/tan.70294","url":null,"abstract":"<div>\u0000 \u0000 <p>Full-length sequence of HLA<i>-C*01:289</i> covers the 5′-untranslated region (UTR), all exons and introns and the 3′ UTR.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144515078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of the Novel HLA-C*15:271N Allele by Next Generation Sequencing","authors":"John Jeongseok Yang, Heung-Bum Oh","doi":"10.1111/tan.70299","DOIUrl":"https://doi.org/10.1111/tan.70299","url":null,"abstract":"<div>\u0000 \u0000 <p><i>HLA-C*15:271N</i> differs from <i>HLA-C*15:02:01:01</i> by an insertion in exon 3 leading to a premature termination codon.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144520196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Novel Allele, HLA-A*03:505, With a Non-Synonymous Mutation in Exon 2","authors":"Maria Loginova, Igor Paramonov, Maksim Zarubin","doi":"10.1111/tan.70311","DOIUrl":"https://doi.org/10.1111/tan.70311","url":null,"abstract":"<div>\u0000 \u0000 <p>HLA-A*03:505 contains a single nucleotide substitution at nucleotide position 236 (GAG to GCG).</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucie Blandin, Mamy Ralazamahaleo, Gwendaline Guidicelli, Paul Rouzaire, Richard Lemal
{"title":"Characterisation of the Novel HLA-C*07:1187 Allele by Sequencing-Based Typing","authors":"Lucie Blandin, Mamy Ralazamahaleo, Gwendaline Guidicelli, Paul Rouzaire, Richard Lemal","doi":"10.1111/tan.70305","DOIUrl":"https://doi.org/10.1111/tan.70305","url":null,"abstract":"<div>\u0000 \u0000 <p>HLA-C*07:1187 differs from HLA-C*07:02:01:01 by one nucleotide substitution in codon 304 in exon 5.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyun-Jun Nam, Jun Young Hong, Sunmi Jung, Jina Lee, Younhee Park
{"title":"Identification of the Novel HLA-C*08:01:36 Allele Using Next-Generation Sequencing","authors":"Hyun-Jun Nam, Jun Young Hong, Sunmi Jung, Jina Lee, Younhee Park","doi":"10.1111/tan.70303","DOIUrl":"https://doi.org/10.1111/tan.70303","url":null,"abstract":"<div>\u0000 \u0000 <p>The <i>HLA-C*08:01:36</i> allele differs from <i>HLA-C*08:01:01:01</i> allele by a single nucleotide at position 42 G > C in exon 1.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ning Xie, Yi Duan, Yuping Yang, Zhihui Feng, Xiuxiang Liu
{"title":"Genomic Full-Length Sequence of the HLA-DQB1*06:47 Allele Was Identified by PacBio Sequencing","authors":"Ning Xie, Yi Duan, Yuping Yang, Zhihui Feng, Xiuxiang Liu","doi":"10.1111/tan.70296","DOIUrl":"https://doi.org/10.1111/tan.70296","url":null,"abstract":"<div>\u0000 \u0000 <p>The complete genomic sequence of <i>HLA-DQB1*06:47</i> was determined using a PacBio sequencing approach conducted in China.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marine Cargou, Julien Lion, Intissar Dalhoumi, Elodie Wojciechowski, Mamy Ralazamahaleo, Gwendaline Guidicelli, Nicolas Guillaume, Jonathan Visentin
{"title":"Deciphering the Interference of the Anti-Integrin α4β7 Vedolizumab With Cell Crossmatch","authors":"Marine Cargou, Julien Lion, Intissar Dalhoumi, Elodie Wojciechowski, Mamy Ralazamahaleo, Gwendaline Guidicelli, Nicolas Guillaume, Jonathan Visentin","doi":"10.1111/tan.70301","DOIUrl":"https://doi.org/10.1111/tan.70301","url":null,"abstract":"<div>\u0000 \u0000 <p>In organ transplantation, immunological risk assessment uses cell crossmatch (XM) results, which can be altered by several drugs. We observed two recipients awaiting kidney and liver transplantation with positive flow cytometry XM (FCXM) on T-cells in the absence of Donor Specific Antibodies (DSA). Both were treated with vedolizumab (VDZ) for an inflammatory bowel disease (IBD). VDZ is an IgG1 directed against integrin α4β7, expressed on T and B cell subsets, we therefore suspected that VDZ interfered with XM. We tested with Luminex screening and single antigen assays and with auto- and allo-FCXM on different HLA-typed cells several sera from the two recipients, collected before and during treatment with VDZ. To assess the intensity and the kinetics of the interference, the sera collected during VDZ treatment were from the induction and the maintenance phases at different times post-injection. All sera were DSA-negative with Luminex assays, indicating the absence of interference of VDZ with virtual XM. IgG allo-FCXM on T cells was positive during the maintenance phase while it was randomly positive on B cells. On T cells, the ratio was between 1.6 and 4.4 times the negative control (positivity threshold of 1.5) for the two recipients, and did not depend on the time between collection and injection. Unique T cell populations express Integrin α4β7, then we did not observe a global shift for T cells in the presence of VDZ but a shift for a small subset which was sufficient to induce a positive FCXM. VDZ treatment did not interfere with complement-dependent cytotoxicity XM or with IgM FCXM. Pronase treatment abrogated VDZ interference. In the event of unexpected positive FCXM on T cells in a recipient with IBD, the transplant team should seek VDZ treatment and interference.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"106 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of the Novel HLA-DPB1*19:01:02 Allele by Next-Generation Sequencing","authors":"Chen Chen, Fang Wang, Wenwen Pi, Wei Zhang, Faming Zhu","doi":"10.1111/tan.70289","DOIUrl":"https://doi.org/10.1111/tan.70289","url":null,"abstract":"<div>\u0000 \u0000 <p>HLA-DPB1*19:01:02 differs from HLA-DPB1*19:01:01 by a single nucleotide substitution at position 735 C>T.</p>\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"105 6","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}