IF 5.9 4区医学

HLA Pub Date : 2024-11-03 DOI: 10.1111/tan.15747

Maria Loginova, Ivan Obukhov, Igor Paramonov

引用次数: 0

Identification of the Novel HLA-A*03:01:01:122 Allele in a Chinese Individual Using PacBio HiFi Sequencing 利用 PacBio HiFi 测序鉴定一名中国人的新型 HLA-A*03:01:01:122 等位基因。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15733

Yue Han, Yankun Li, Yuqi Wang, Juan Ren, Xiaoning Wang

引用次数: 0

Characterisation of the Novel HLA-A*02:406 Allele by Sequencing-Based Typing in a Chinese Individual 通过基于测序的分型鉴定一名中国人的新型 HLA-A*02:406 等位基因。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15739

Jian-Ping Li, Ying-Dong Wei, Xiao-Feng Li

引用次数: 0

The Novel HLA-B*15:693 Allele Identified by Next-Generation Sequencing 通过下一代测序发现的新型 HLA-B*15:693 等位基因

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15730

Olivia S. E. Davies, Nicola Konzett, Livia Prantl, Anita Siller, Anja Vales

引用次数: 0

Identification of the Novel HLA-A*02:407 Allele by Sanger Dideoxy Nucleotide Sequencing 通过桑格双脱氧核苷酸测序鉴定新型 HLA-A*02:407 等位基因

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15737

Xiao-Feng Li, Xu Zhang, Feng-Qiu Lin, Min-Jie Wei, Jian-Ping Li

引用次数: 0

The Novel HLA-DRB1*03:218N Allele, Identified by Next-Generation Sequencing in a Greek Individual 通过下一代测序在一名希腊人身上发现新的 HLA-DRB1*03:218N 等位基因。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15744

Theofilos Athanassiades, Diamanto Kouniaki, Vasiliki Kitsiou, Alexandra Tsirogianni

引用次数: 0

Next Generation Sequencing Identifies Two Novel HLA-B Alleles, HLA-B*37:113 and HLA-B*41:02:12 新一代测序鉴定出两种新型 HLA-B 等位基因：HLA-B*37:113 和 HLA-B*41:02:12。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15742

Maria Loginova, Ivan Obukhov, Igor Paramonov

引用次数: 0

Establishment and Application of a Multiplex PCR NGS Method for the Genotyping of HLA-Class I and HPA 用于 HLA I 类和 HPA 基因分型的多重 PCR NGS 方法的建立和应用。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15716

Yanmin He, Fang Wang, Zhipan Wu, Wei Zhang, Faming Zhu

{"title":"Establishment and Application of a Multiplex PCR NGS Method for the Genotyping of HLA-Class I and HPA","authors":"Yanmin He, Fang Wang, Zhipan Wu, Wei Zhang, Faming Zhu","doi":"10.1111/tan.15716","DOIUrl":"10.1111/tan.15716","url":null,"abstract":"<div>\u0000 \u0000 Selecting compatible HLA-Class I and/or HPA platelets based on genotyping could alleviate immune platelet transfusion refractoriness (PTR). A fast and reliable method of HLA-Class I and HPA genotyping is necessary to construct a platelet donor bank with known HLA-Class I and HPA genotypes. Ten pairs of specific primers for HLA-A, HLA-B, HLA-C, HPA-1 through HPA-6w, HPA-15 and HPA-21w were designed. The appropriate fragments were optimised for amplification in a single multiplex reaction. After a cleanup step using paramagnetic beads, the amplicon library was prepared and sequenced. To validate the accuracy of the developed method, commercial NGS kits for the genotyping of HLA-A, HLA-B and HLA-C and the TaqMan real-time PCR method in-house for the genotyping of HPA-1 through HPA-6w, HPA-15 and HPA-21w were used to detect all the specimens in parallel. A total of 386 specimens were detected and the results of genotyping HLA-A, HLA-B, HLA-C and HPA-1 through HPA-6w, HPA-15 and HPA-21w were obtained simultaneously, which is 100% consistent between the two methods. Four new HLA alleles, HLA-A*11:451, HLA-A*30:01:26, HLA-B*39:201 and HLA-B*40:538, were also reconfirmed. Two novel SNVs, c.2671C > T and c.2681T > G, in the coding region of ITGA2B were detected, all of which are heterozygous in individuals. A novel NGS method based on multiplex PCR was established to detect HLA-Class I and HPA simultaneously, which is high-throughput, rapid and accurate and could be applied to build a platelet donor bank.\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"104 4","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel HLA-B*15 Variant Allele, HLA-B*15:359, Identified by Sequencing-Based Typing in a Chinese Individual 在一名中国人身上通过基于测序的分型鉴定出一个新的 HLA-B*15 变异等位基因 HLA-B*15:359。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15745

Xiao-Feng Li, Xu Zhang, Feng-Qiu Lin, Jian-Ping Li

引用次数: 0

Distributions of MICA and MICB Alleles Typed by Amplicon-Based Next-Generation Sequencing in South Koreans 通过基于扩增子的下一代测序技术分析韩国人的 MICA 和 MICB 等位基因分布情况。

IF 5.9 4区医学

HLA Pub Date : 2024-10-29 DOI: 10.1111/tan.15735

Eun-Jeong Choi, Hyoung-Jae Kim, Jin-Hyeok Kim, In-Cheol Baek

{"title":"Distributions of MICA and MICB Alleles Typed by Amplicon-Based Next-Generation Sequencing in South Koreans","authors":"Eun-Jeong Choi, Hyoung-Jae Kim, Jin-Hyeok Kim, In-Cheol Baek","doi":"10.1111/tan.15735","DOIUrl":"10.1111/tan.15735","url":null,"abstract":"<div>\u0000 \u0000 Major histocompatibility complex class I chain-related genes A and B (MICA and MICB) play a role as ligands in activating the NKG2D receptor expressed in natural killer cells, γδ T-cells and αβ CD8 T-cells and have been defined in human diseases and haematopoietic stem cell transplantation (HSCT). MICA and MICB alleles were genotyped at the three-field level by amplicon-based next-generation sequencing (NGS) using a MiSeqDx system and compared with the results from previous studies in healthy South Korean donors. Exons 2–5 of MICA and exons 2–4 of MICB were amplified using a multiplex polymerase chain reaction (PCR). Sequence reads of ≥ 51 depth counts were consistently obtained for each sample exon, and target exons were determined to match reference sequences contained in the IPD-IMGT/HLA database. MICA and MICB alleles were tested using exon combinations. The program was designed to recognise specific sequences and discriminate between the MICA*008:01:01/*027 alleles. A total of 22 alleles were found in MICA and MICB. We observed 1 HLA-C ~ HLA-B ~ MICA ~ MICB ~ HLA-DRB1 haplotype with significant linkage disequilibrium between alleles at all neighbouring HLA loci. These results are consistent with previous microarray results. Genotyping of MICA and MICB was possible using 11-loci HLA genes. We updated the distribution of MICA and MICB based on three-field allele and haplotype frequencies containing linkage disequilibrium in South Koreans using amplicon-based NGS. These data suggest that high-resolution MICA and MICB typing data obtained using NGS may aid in performing HSCT and disease association studies.\u0000 </div>","PeriodicalId":13172,"journal":{"name":"HLA","volume":"104 4","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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