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Publisher Correction: Comparing long-term changes in cladoceran and diatom assemblages from a lake impacted by road salt seepage to a nearby reference lake near Toronto (Ontario, Canada) 出版商更正:比较多伦多(加拿大安大略省)附近一个受路面盐渗漏影响的湖泊与附近一个参照湖泊中底栖动物和硅藻群的长期变化
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-12 DOI: 10.1007/s10750-024-05682-4
R. E. Valleau, K. G. Murray, A. M. Paterson, J. P. Smol
{"title":"Publisher Correction: Comparing long-term changes in cladoceran and diatom assemblages from a lake impacted by road salt seepage to a nearby reference lake near Toronto (Ontario, Canada)","authors":"R. E. Valleau, K. G. Murray, A. M. Paterson, J. P. Smol","doi":"10.1007/s10750-024-05682-4","DOIUrl":"https://doi.org/10.1007/s10750-024-05682-4","url":null,"abstract":"","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"21 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparing seasonal heterogeneity of phytoplankton habitat and community in northern lakes with low to moderate but historically variable DOC concentrations 比较 DOC 浓度从低到中等但历史上可变的北方湖泊中浮游植物生境和群落的季节异质性
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-11 DOI: 10.1007/s10750-024-05694-0
Matthew J. Farragher, Václava Hazuková, William G. Gawley, Jasmine E. Saros
{"title":"Comparing seasonal heterogeneity of phytoplankton habitat and community in northern lakes with low to moderate but historically variable DOC concentrations","authors":"Matthew J. Farragher, Václava Hazuková, William G. Gawley, Jasmine E. Saros","doi":"10.1007/s10750-024-05694-0","DOIUrl":"https://doi.org/10.1007/s10750-024-05694-0","url":null,"abstract":"<p>Dissolved organic matter (DOM) influences several physical and chemical drivers of phytoplankton habitat. Increasing variability in lakewater dissolved organic carbon (DOC) concentrations in recent decades has raised uncertainty over temporal responses of phytoplankton communities to changing DOM. We conducted limnological surveys in four lakes with low (~ 2 mg l<sup>−1</sup>) to moderate (~ 4 mg l<sup>−1</sup>) DOC in Maine, USA, from February to November 2020 to assess variability in phytoplankton metrics and habitat gradients. Low-DOC lakes had similar thermal structure patterns compared to moderate-DOC lakes, but more stable euphotic depths. While moderate-DOC lakes had greater seasonal heterogeneity of biomass and vertical distribution of chlorophyll-a, the low-DOC lake had greater phytoplankton community turnover (Morisita-Horn Dissimilarity Index), underscored by a shift in dominance from autotrophs in the winter under ice to mixotrophs throughout the open water season. Long-term trends showed DOC concentrations generally increased for one decade beginning in 1995, followed by decreases in all four lakes for 12–15 years until 2020 by rates of 0.02–0.15 mg l<sup>−1</sup> year<sup>−1</sup>, during which time water clarity increased in one lake. Small differences in or changes to DOC elicit greater variability of phytoplankton in lakes with low to moderate DOC concentrations.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"63 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scale effects of riparian forests on fish diversity in streams of the upper Paraná River basin 河岸森林对巴拉那河上游流域鱼类多样性的规模效应
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-11 DOI: 10.1007/s10750-024-05699-9
Leonardo Antunes Pessoa, Edivando Vitor do Couto, João Paulo Alves Pagotto, Erivelto Goulart
{"title":"Scale effects of riparian forests on fish diversity in streams of the upper Paraná River basin","authors":"Leonardo Antunes Pessoa, Edivando Vitor do Couto, João Paulo Alves Pagotto, Erivelto Goulart","doi":"10.1007/s10750-024-05699-9","DOIUrl":"https://doi.org/10.1007/s10750-024-05699-9","url":null,"abstract":"<p>Despite the ecological importance of riparian forests to streams, the specific scale at which forest cover most significantly influences fish assemblages remains poorly understood. To address this issue, we measured the percentage of forest cover at 11 spatial scales across 12 streams. These scales were categorized into: “local riparian”, which includes circular buffers around the sampled sections, and “riparian network”, which consists of buffers surrounding the stream network upstream of the sampled sections. We developed linear models using species diversity, based on Hill numbers, and the percentage of forest cover at each scale. Additionally, we included the terrain slope and the dominant type of land use in the watershed (rural or urban) as covariates. The results indicated that the 100-m buffer of the local riparian scale was the most effective for assessing fish diversity. The percentage of forest cover in this buffer had a positive and significant relationship with the diversity metrics, though terrain slope significantly influenced this relationship. Therefore, we recommend that studies evaluating the influence of landscape on fish assemblages in streams adopt a multiscale approach to avoid missing the true impact of the landscape on the assemblages.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"85 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The entrainment of polyester microfibers modifies the structure and function of periphytic biofilms 聚酯微纤维的夹带改变了附生生物膜的结构和功能
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-10 DOI: 10.1007/s10750-024-05678-0
Mitchell J. Liddick, Steven T. Rier
{"title":"The entrainment of polyester microfibers modifies the structure and function of periphytic biofilms","authors":"Mitchell J. Liddick, Steven T. Rier","doi":"10.1007/s10750-024-05678-0","DOIUrl":"https://doi.org/10.1007/s10750-024-05678-0","url":null,"abstract":"<p>As plastics undergo degradation, they give rise to microplastics (MPs), such as polyester microfibers (PMFs), which are increasingly recognized for their potential impact on microbial communities. Despite a growing body of the literature on MP effects, there is a gap in understanding prolonged PMF exposure (≥ 1 month) on stream periphyton across an extensive concentration gradient. This study addresses this gap by investigating the response of periphyton exposed to increasing PMF concentrations (0–22,000 PMF L<sup>−1</sup>) in stream mesocosms. As PMF concentrations increased, total periphyton biomass remained unaffected, while algal and bacterial biomass decreased and increased, respectively. Higher PMF concentrations also modified coarse algal community structure (measured as changes in chlorophyll <i>b</i>:<i>c</i>) and decreased light harvesting efficiency. Increased bacterial abundance was accompanied by elevated respiration, shifting the system from net autotrophy to net heterotrophy at 10,000–12,000 PMF L<sup>−1</sup>. Additionally, bacterial community composition was altered along with reductions in <i>β</i>-1,4-glucosidase activities. Despite reduced algal biomass, higher PMF concentrations appeared to support bacterial growth. Many periphyton attributes, including nutrient composition, phosphorus removal, gross primary production, and maximum electron transport rate of photosystem II, were unaffected. This study underscores the multifaceted implications of PMF contamination on the structure and function of periphyton in stream ecosystems.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"4 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Demographic responses of an extremophile crustacean to environmental factors: Great Salt Lake (Utah, USA) brine shrimp (Artemia franciscana) 嗜极甲壳动物对环境因素的繁殖反应:大盐湖(美国犹他州)卤虾(Artemia franciscana)
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-06 DOI: 10.1007/s10750-024-05684-2
Gary E. Belovsky, Chad A. Larson, Heidi K. Mahon, Chad Mellison, Andrea C. Stumpf, Anghy Ramos Valencia
{"title":"Demographic responses of an extremophile crustacean to environmental factors: Great Salt Lake (Utah, USA) brine shrimp (Artemia franciscana)","authors":"Gary E. Belovsky, Chad A. Larson, Heidi K. Mahon, Chad Mellison, Andrea C. Stumpf, Anghy Ramos Valencia","doi":"10.1007/s10750-024-05684-2","DOIUrl":"https://doi.org/10.1007/s10750-024-05684-2","url":null,"abstract":"<p>Hypersaline Great Salt Lake’s (GSL: Utah, USA) pelagic food web is dominated by the herbivore, <i>Artemia franciscana</i>. <i>Artemia</i> demographic responses (survival, developmental transition, and reproduction) to GSL salinities, temperatures, common phytoplankton and yeast, and food levels were examined by factorial experiment. Survival across developmental stages was best at 90 ppt salinity, and decreased as temperature increased. Transition between life stages was best at 45 ppt salinity, and increased as temperature increased. Food was most important with both survival and transitioning responding similarly to food types and increasing with amount of food. <i>Artemia</i> reproduce in two ways (diapausing cysts – oviparity, live young – ovoviviparity): ovoviviparous and total reproduction were greatest at 90 ppt salinity and 20 °C, while oviparous reproduction was weakly affected by salinity and greatest at 20 °C. Oviparity was greatest at low food availability, while ovoviviparity and total reproduction increased with food availability, so reproduction shifted from oviparity to ovoviviparity as food increased. Maternal effects were observed for cyst hatchability, and ovoviviparous nauplii survival and transitioning to the juvenile stage. Combinations of salinity, temperature, food taxa and food amount strongly affect demography, making single factor studies of limited value. Results explain <i>Artemia</i> abundance in different parts of GSL and among years.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"85 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of floating photovoltaics on aquatic organisms: a review 漂浮光伏发电对水生生物的影响:综述
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-05 DOI: 10.1007/s10750-024-05686-0
Paula Mazza Barbosa Oliveira, Rafael Marques Almeida, Simone Jaqueline Cardoso
{"title":"Effects of floating photovoltaics on aquatic organisms: a review","authors":"Paula Mazza Barbosa Oliveira, Rafael Marques Almeida, Simone Jaqueline Cardoso","doi":"10.1007/s10750-024-05686-0","DOIUrl":"https://doi.org/10.1007/s10750-024-05686-0","url":null,"abstract":"<p>Solar photovoltaic (PV) generation is burgeoning as global economies pursue decarbonization goals. To meet the surge in solar energy demand, deployment of PV panels on water surfaces has emerged as an attractive option. Despite the potential advantages associated with floating PV (FPV) systems, current understanding of their impact on aquatic life remains scarce. Here, we use the Preferred Reporting Items for Systematic Review and Meta-analysis methodology to provide a systematic review of FPV effects on aquatic organisms. Our search yielded 24 studies selected for qualitative analysis—most of which were performed in small-scale artificial ecosystems. We identified 39 variables across 12 artificial taxonomic groups. The most commonly reported effect was the reduction of chlorophyll-a in the water. Additionally, there is growing evidence that FPV has indirect effects on various groups of aquatic organisms. Enhancing current understanding of ecological consequences of FPV systems on aquatic biodiversity is urgent given the industry’s rapid expansion, and developing strategies for monitoring, controlling and predicting water quality impacts is necessary. This review provides a foundation for future studies and can assist in the development of strategies for balancing impacts and benefits of FPV systems.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"11 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The World Register of Marine Species (WoRMS) through the looking glass: insights from the data management team in light of the crystal anniversary of WoRMS 透过玻璃看世界海洋物种名录(WoRMS):数据管理团队在世界海洋物种名录水晶周年纪念之际发表的见解
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-04 DOI: 10.1007/s10750-024-05688-y
Leen Vandepitte, Stefanie Dekeyzer, Wim Decock, Lynn Delgat, Ben Boydens, Lennert Tyberghein, Bart Vanhoorne, Francisco Hernandez, Jan Mees, Tammy Horton, Andreas Kroh, Christopher B. Boyko, Shane T. Ahyong
{"title":"The World Register of Marine Species (WoRMS) through the looking glass: insights from the data management team in light of the crystal anniversary of WoRMS","authors":"Leen Vandepitte, Stefanie Dekeyzer, Wim Decock, Lynn Delgat, Ben Boydens, Lennert Tyberghein, Bart Vanhoorne, Francisco Hernandez, Jan Mees, Tammy Horton, Andreas Kroh, Christopher B. Boyko, Shane T. Ahyong","doi":"10.1007/s10750-024-05688-y","DOIUrl":"https://doi.org/10.1007/s10750-024-05688-y","url":null,"abstract":"<p>The World Register of Marine Species (WoRMS) started in 2007 with the question “how many species live in our oceans?”. Now, a little over 15 years later, WoRMS is able to answer several questions related to marine species discovery rates and provides a dynamic number of existing marine species, based on the information provided by hundreds of taxonomic experts worldwide, who have proven to be diverse and dynamic. We present basic statistics on marine species discovery rates based on the currently available content of WoRMS, as well as insights in the day-to-day activities and dynamics of our editorial board and the progress made so far on the content priorities as defined by the WoRMS Steering Committee. As for all dynamic systems, WoRMS is not complete and faces challenges. As an endorsed project of the UN Ocean Decade, WoRMS aims to tackle a number of these challenges and knowledge-gaps by 2030, including detailed documentation of authorships and original descriptions, and will provide continuous support to all marine initiatives, programs and projects that rely on WoRMS as an authoritative classification and catalogue of marine names.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"41 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrating real-time monitoring and ecotoxicology using a neotropical stream as a study case 以新热带溪流为研究案例,将实时监测与生态毒理学结合起来
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-04 DOI: 10.1007/s10750-024-05687-z
Edimar Olegário de Campos Júnior, Boscolli Barbosa Pereira, Nathan Oliveira Barros
{"title":"Integrating real-time monitoring and ecotoxicology using a neotropical stream as a study case","authors":"Edimar Olegário de Campos Júnior, Boscolli Barbosa Pereira, Nathan Oliveira Barros","doi":"10.1007/s10750-024-05687-z","DOIUrl":"https://doi.org/10.1007/s10750-024-05687-z","url":null,"abstract":"<p>This study pioneers the evaluation of toxicity in the Coromandel stream, located in the southeastern region of Brazil, highlighting the essential role of ecotoxicology in informing environmental management decisions. We employed bioindicators to assess the water quality across multiple collection points along the stream, focusing on its physical, chemical, and ecotoxicological characteristics. The investigation utilized <i>Allium cepa</i> seeds and <i>Dendrocephalus brasiliensis</i> nauplii to conduct acute ecotoxicological tests, examining cytotoxic and genotoxic effects, including the mitotic index and micronuclei frequency for <i>A. cepa</i> and immobility rates for <i>D. brasiliensis</i>. Our findings reveal significant pollution impacts, particularly in areas affected by residential and industrial activities, where increased immobility in <i>D. brasiliensis</i> and heightened genotoxic potential were observed. These outcomes indicate deteriorating water quality and underscore the urgency for targeted environmental interventions. Real-time monitoring further identified critical alterations in water parameters, underscoring the stream sections with significant anthropogenic influence as hotspots for pollution and toxic risk. This research underscores the importance of integrated ecotoxicological assessments in detecting and addressing environmental threats in freshwater ecosystems.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"40 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The freshwaters bivalves of La Plata Basin in the Anthropocene 人类世的拉普拉塔盆地淡水双壳类动物
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-04 DOI: 10.1007/s10750-024-05679-z
Cristhian Clavijo, Igor Christo Miyahira, Agustín Bassó
{"title":"The freshwaters bivalves of La Plata Basin in the Anthropocene","authors":"Cristhian Clavijo, Igor Christo Miyahira, Agustín Bassó","doi":"10.1007/s10750-024-05679-z","DOIUrl":"https://doi.org/10.1007/s10750-024-05679-z","url":null,"abstract":"<p>La Plata Basin, the fifth-largest basin in the world, includes areas of Argentina, Bolivia, Brazil, Paraguay, and Uruguay and is subjected to intensive human activities such as agriculture, mining, and global trade. The basin hosts 83 native bivalves (Hyriidae, Mycetopodidae, Cyrenoididae, and Sphaeriidae), including 29 endemic and at least 3 non-native species (Cyrenidae and Mytilidae). For their role as filter feeders and their dominance in biomass in benthic freshwater ecosystems, freshwater bivalves play a key role in the resilience of aquatic ecosystems. In this review, we discuss the six major global change threats to freshwater ecosystems in the Anthropocene (climate change, flow regulation, pollution, land-use change, invasive species, and overexploitation) using freshwater bivalves of La Plata Basin as a model in South America. Future directions to properly understand the effects of global change and suggestions for the conservation of the freshwater bivalves in the basin are stated.</p>","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"3 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Synopsis of the European Freshwater Mussels: Pseudunio auricularius (Spengler, 1793), the Giant Freshwater Pearl Mussel 更正:欧洲淡水贻贝简介:Pseudunio auricularius (Spengler, 1793),巨型淡水珍珠贻贝
IF 2.6 3区 生物学
Hydrobiologia Pub Date : 2024-09-04 DOI: 10.1007/s10750-024-05681-5
Keiko Nakamura, Karl M. Wantzen, Joaquin Soler, Ronaldo Sousa, Karl-Otto Nagel, David C. Aldridge, Manuel Lopes-Lima, Vincent Prié
{"title":"Correction: Synopsis of the European Freshwater Mussels: Pseudunio auricularius (Spengler, 1793), the Giant Freshwater Pearl Mussel","authors":"Keiko Nakamura, Karl M. Wantzen, Joaquin Soler, Ronaldo Sousa, Karl-Otto Nagel, David C. Aldridge, Manuel Lopes-Lima, Vincent Prié","doi":"10.1007/s10750-024-05681-5","DOIUrl":"https://doi.org/10.1007/s10750-024-05681-5","url":null,"abstract":"","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"44 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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