Hydrobiologia最新文献

In situ estimation of phytoplankton community growth rate inside dialysis membrane bags: a bioassay experiment at a fish farm in the eastern Aegean Sea 透析膜袋内浮游植物群落生长率的现场估算：东爱琴海养鱼场的生物测定实验

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05643-x

Betül Bardakcı Şener, Eyüp Mümtaz Tıraşın

{"title":"In situ estimation of phytoplankton community growth rate inside dialysis membrane bags: a bioassay experiment at a fish farm in the eastern Aegean Sea","authors":"Betül Bardakcı Şener, Eyüp Mümtaz Tıraşın","doi":"10.1007/s10750-024-05643-x","DOIUrl":"https://doi.org/10.1007/s10750-024-05643-x","url":null,"abstract":"We conducted a study to investigate the potential effects of nutrients released from a fish farm, which fell within the typical range found in oligotrophic offshore waters of the Aegean Sea, on phytoplankton growth. We designed an in situ bioassay experiment at a fish farm and incubated natural phytoplankton assemblages inside dialysis membrane bags for six days. Changes in phytoplankton growth in samples of ambient seawater collected throughout the experiment served as controls and were considered indicative of the net population change rates. Half of the bags were filled with seawater filtered through a 150-µm mesh, while the other half contained unfiltered seawater. The growth rates, estimated based on chlorophyll a concentrations and phytoplankton cell numbers inside the filtered and unfiltered bags, showed no significant differences. While no detectable net phytoplankton growth occurred in the ambient seawater, there was an exponential increase in chlorophyll a content and cell numbers within the bags. Moreover, the species richness within the bags gradually declined throughout the experiment. The findings of the study confirm that continuous nutrient releases from fish farms can promote high population growth rates in oligotrophic environments, provided that phytoplankton losses due to grazing, advection, and sinking are minimized or eliminated.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"20 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whistle structure variation between two sympatric dolphin species in the Gulf of California 加利福尼亚湾两种同域海豚的哨音结构变异

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05675-3

Simone Antichi, Manali Rege‑Colt, Maia Austin, Laura J. May‑Collado, Óscar Carlón-Beltrán, Jorge Urbán R., Sergio Martínez-Aguilar, Lorena Viloria-Gómora

{"title":"Whistle structure variation between two sympatric dolphin species in the Gulf of California","authors":"Simone Antichi, Manali Rege‑Colt, Maia Austin, Laura J. May‑Collado, Óscar Carlón-Beltrán, Jorge Urbán R., Sergio Martínez-Aguilar, Lorena Viloria-Gómora","doi":"10.1007/s10750-024-05675-3","DOIUrl":"https://doi.org/10.1007/s10750-024-05675-3","url":null,"abstract":"Dolphins produce narrowband and frequency modulated sounds called whistles during a variety of behavioral contexts. Dolphin species vary in their whistle contour composition, frequency range, modulation, and duration, and these differences can be useful in their identification. Here, we compare the whistle contours of two sympatric dolphin species at La Paz Bay, Gulf of California, the Eastern Tropical Pacific bottlenose dolphin, and the long-beaked common dolphin. Dolphins were recorded (7 h and 1 min recording effort) from the research vessel with the engine off using an over-the-side hydrophone and a broadband recording system. A total of 666 high quality whistles (bottlenose dolphin, n = 415; long-beaked common dolphin, n = 251) were analyzed. A Random Forest Analysis identified duration, ending frequency, and maximum frequency as the most important variables that distinguish the two dolphin species. The most common whistle contour types of bottlenose dolphins were sine and convex, while the long-beaked common dolphins produced mainly upsweep and concave whistles. The results showed that bottlenose dolphins and long-beaked common dolphins differ in their whistle variables and contours facilitating their identification in future passive acoustic studies.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"16 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitology of one of the world’s foremost fisheries target species lacks a One Health approach 世界上最重要的渔业目标物种之一的寄生虫学缺乏 "一体健康 "方法

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05695-z

Miriam Isoyi Shigoley, Nicolas Antoine-Moussiaux, Thierry Jauniaux, Maarten P. M. Vanhove

{"title":"Parasitology of one of the world’s foremost fisheries target species lacks a One Health approach","authors":"Miriam Isoyi Shigoley, Nicolas Antoine-Moussiaux, Thierry Jauniaux, Maarten P. M. Vanhove","doi":"10.1007/s10750-024-05695-z","DOIUrl":"https://doi.org/10.1007/s10750-024-05695-z","url":null,"abstract":"The global demand for affordable animal protein, particularly Nile tilapia, has driven increased adoption of (semi-)intensive farming practices. This intensification poses challenges like fish disease outbreaks, higher parasite loads, increased mortality rates, and environmental degradation. Addressing these issues requires a comprehensive understanding of the biology and ecology of these disrupted equilibria, emphasizing the need to characterize parasites, their pathogenic effects, and the conditions facilitating their emergence. Despite 276 known parasite species infecting Nile tilapia, existing reports are fragmented, often conducted locally or focused on a few species in experimental settings. A timely challenge is summarizing the state of knowledge and presenting links between human, animal, and environmental health. Unfortunately, limited studies focus on these parasites’ actual effects and environmental correlates, indicating little research effort. Comparing the number of parasite species described with few studies documenting their impacts reveals large knowledge gaps. The current information on these parasites lacks practical applicability for stakeholders in production and management. Bridging this knowledge gap requires both descriptive and experimental studies. Adopting the One Health approach in parasitological assessments and conducting further research will ensure aquaculture stakeholders can access valuable information for informed decision-making, prioritizing environmental integrity, fish health and welfare, and consumer well-being.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"189 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing agricultural land use in riparian networks negatively affects stream fish communities in a tropical savanna 河岸网络中农业用地的增加对热带稀树草原的溪流鱼类群落产生负面影响

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-13 DOI: 10.1007/s10750-024-05697-x

Jenny J. Morales, Lúcia Mateus, Luiza Peluso, Peter Zeilhofer, Bruno R. S. Figueiredo, Jerry Penha

{"title":"Increasing agricultural land use in riparian networks negatively affects stream fish communities in a tropical savanna","authors":"Jenny J. Morales, Lúcia Mateus, Luiza Peluso, Peter Zeilhofer, Bruno R. S. Figueiredo, Jerry Penha","doi":"10.1007/s10750-024-05697-x","DOIUrl":"https://doi.org/10.1007/s10750-024-05697-x","url":null,"abstract":"The Brazilian Cerrado has been experiencing intense land use changes for decades, with large-scale conversion of its natural vegetation to agriculture and livestock grazing. These agents of landscape change also affect the aquatic ecosystems embedded in the biome. Here, we investigated the effects of land use at different scales (catchment, riparian network, and local riparian) on fish assemblages in streams of the Brazilian Cerrado. We hypothesized that increasing agricultural land use (ALU%) reduces fish diversity and the compositional uniqueness of the fish assemblage. We used GLM to evaluate the impact of low-intensity ALU% on fish richness and abundance. Additionally, we employed beta regression to analyze the effect on local contribution to beta diversity (LCBD) and redundancy analysis to assess the impact on species composition. Our results showed that the effects of low-intensity ALU% depend on the spatial scale. While increasing ALU% in the riparian network negatively affected LCBD and altered species composition, ALU% in the catchment had negligible to slightly positive effects on LCDB, within the range of ALU% considered. Our findings reinforce the need to preserve riparian forests to maintain beta diversity in the streams of the Brazilian Cerrado. Also, no evidence was found that low-intense catchment use, without degrading the riparian forest, affects fish assemblages.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"11 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of phytoplankton diversity using microscopy and metabarcoding: insights from an eLTER station in the Northern Adriatic Sea 利用显微镜和代谢编码对浮游植物多样性进行比较分析：北亚得里亚海 eLTER 站的启示

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-12 DOI: 10.1007/s10750-024-05692-2

Francesca Neri, Marika Ubaldi, Stefano Accoroni, Sara Ricci, Elisa Banchi, Tiziana Romagnoli, Cecilia Totti

{"title":"Comparative analysis of phytoplankton diversity using microscopy and metabarcoding: insights from an eLTER station in the Northern Adriatic Sea","authors":"Francesca Neri, Marika Ubaldi, Stefano Accoroni, Sara Ricci, Elisa Banchi, Tiziana Romagnoli, Cecilia Totti","doi":"10.1007/s10750-024-05692-2","DOIUrl":"https://doi.org/10.1007/s10750-024-05692-2","url":null,"abstract":"The monitoring of phytoplankton is crucial to highlight changes in the marine ecosystems. In the present study, the phytoplankton community of an eLTER station in the Northern Adriatic Sea was analysed combining two approaches, i.e. microscopy and eDNA metabarcoding (targeting V4 and V9 regions of the 18S rRNA gene, and using PR2 and SILVA as reference databases), to highlight the strengths and weaknesses of these two methods. Metabarcoding revealed a so far unknown phytoplankton diversity (99 genera and 151 species), while microscopy detected 14 genera and 44 species not revealed by metabarcoding. Only a small percentage of genera and species were shared by the two methods (microscopy and metabarcoding), 18S regions (V4 and V9) and reference databases (PR2 and SILVA). Metabarcoding showed a community characterized by a higher number of phytoflagellate and dinoflagellate genera and species, in comparison with microscopy where diatom and dinoflagellate taxa were the most represented. Moreover, metabarcoding failed to reveal almost all the coccolithophores. The results confirmed metabarcoding as a powerful tool, but it should still be combined with microscopy to have a more detailed information on the community and to counteract the drawbacks of metabarcoding, such as gaps in the reference databases.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"7 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Publisher Correction: Comparing long-term changes in cladoceran and diatom assemblages from a lake impacted by road salt seepage to a nearby reference lake near Toronto (Ontario, Canada) 出版商更正：比较多伦多（加拿大安大略省）附近一个受路面盐渗漏影响的湖泊与附近一个参照湖泊中底栖动物和硅藻群的长期变化

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-12 DOI: 10.1007/s10750-024-05682-4

R. E. Valleau, K. G. Murray, A. M. Paterson, J. P. Smol

引用次数: 0

Comparing seasonal heterogeneity of phytoplankton habitat and community in northern lakes with low to moderate but historically variable DOC concentrations 比较 DOC 浓度从低到中等但历史上可变的北方湖泊中浮游植物生境和群落的季节异质性

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-11 DOI: 10.1007/s10750-024-05694-0

Matthew J. Farragher, Václava Hazuková, William G. Gawley, Jasmine E. Saros

{"title":"Comparing seasonal heterogeneity of phytoplankton habitat and community in northern lakes with low to moderate but historically variable DOC concentrations","authors":"Matthew J. Farragher, Václava Hazuková, William G. Gawley, Jasmine E. Saros","doi":"10.1007/s10750-024-05694-0","DOIUrl":"https://doi.org/10.1007/s10750-024-05694-0","url":null,"abstract":"Dissolved organic matter (DOM) influences several physical and chemical drivers of phytoplankton habitat. Increasing variability in lakewater dissolved organic carbon (DOC) concentrations in recent decades has raised uncertainty over temporal responses of phytoplankton communities to changing DOM. We conducted limnological surveys in four lakes with low (~ 2 mg l−1) to moderate (~ 4 mg l−1) DOC in Maine, USA, from February to November 2020 to assess variability in phytoplankton metrics and habitat gradients. Low-DOC lakes had similar thermal structure patterns compared to moderate-DOC lakes, but more stable euphotic depths. While moderate-DOC lakes had greater seasonal heterogeneity of biomass and vertical distribution of chlorophyll-a, the low-DOC lake had greater phytoplankton community turnover (Morisita-Horn Dissimilarity Index), underscored by a shift in dominance from autotrophs in the winter under ice to mixotrophs throughout the open water season. Long-term trends showed DOC concentrations generally increased for one decade beginning in 1995, followed by decreases in all four lakes for 12–15 years until 2020 by rates of 0.02–0.15 mg l−1 year−1, during which time water clarity increased in one lake. Small differences in or changes to DOC elicit greater variability of phytoplankton in lakes with low to moderate DOC concentrations.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"63 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scale effects of riparian forests on fish diversity in streams of the upper Paraná River basin 河岸森林对巴拉那河上游流域鱼类多样性的规模效应

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-11 DOI: 10.1007/s10750-024-05699-9

Leonardo Antunes Pessoa, Edivando Vitor do Couto, João Paulo Alves Pagotto, Erivelto Goulart

{"title":"Scale effects of riparian forests on fish diversity in streams of the upper Paraná River basin","authors":"Leonardo Antunes Pessoa, Edivando Vitor do Couto, João Paulo Alves Pagotto, Erivelto Goulart","doi":"10.1007/s10750-024-05699-9","DOIUrl":"https://doi.org/10.1007/s10750-024-05699-9","url":null,"abstract":"Despite the ecological importance of riparian forests to streams, the specific scale at which forest cover most significantly influences fish assemblages remains poorly understood. To address this issue, we measured the percentage of forest cover at 11 spatial scales across 12 streams. These scales were categorized into: “local riparian”, which includes circular buffers around the sampled sections, and “riparian network”, which consists of buffers surrounding the stream network upstream of the sampled sections. We developed linear models using species diversity, based on Hill numbers, and the percentage of forest cover at each scale. Additionally, we included the terrain slope and the dominant type of land use in the watershed (rural or urban) as covariates. The results indicated that the 100-m buffer of the local riparian scale was the most effective for assessing fish diversity. The percentage of forest cover in this buffer had a positive and significant relationship with the diversity metrics, though terrain slope significantly influenced this relationship. Therefore, we recommend that studies evaluating the influence of landscape on fish assemblages in streams adopt a multiscale approach to avoid missing the true impact of the landscape on the assemblages.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"85 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The entrainment of polyester microfibers modifies the structure and function of periphytic biofilms 聚酯微纤维的夹带改变了附生生物膜的结构和功能

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-10 DOI: 10.1007/s10750-024-05678-0

Mitchell J. Liddick, Steven T. Rier

{"title":"The entrainment of polyester microfibers modifies the structure and function of periphytic biofilms","authors":"Mitchell J. Liddick, Steven T. Rier","doi":"10.1007/s10750-024-05678-0","DOIUrl":"https://doi.org/10.1007/s10750-024-05678-0","url":null,"abstract":"As plastics undergo degradation, they give rise to microplastics (MPs), such as polyester microfibers (PMFs), which are increasingly recognized for their potential impact on microbial communities. Despite a growing body of the literature on MP effects, there is a gap in understanding prolonged PMF exposure (≥ 1 month) on stream periphyton across an extensive concentration gradient. This study addresses this gap by investigating the response of periphyton exposed to increasing PMF concentrations (0–22,000 PMF L−1) in stream mesocosms. As PMF concentrations increased, total periphyton biomass remained unaffected, while algal and bacterial biomass decreased and increased, respectively. Higher PMF concentrations also modified coarse algal community structure (measured as changes in chlorophyll b:c) and decreased light harvesting efficiency. Increased bacterial abundance was accompanied by elevated respiration, shifting the system from net autotrophy to net heterotrophy at 10,000–12,000 PMF L−1. Additionally, bacterial community composition was altered along with reductions in β-1,4-glucosidase activities. Despite reduced algal biomass, higher PMF concentrations appeared to support bacterial growth. Many periphyton attributes, including nutrient composition, phosphorus removal, gross primary production, and maximum electron transport rate of photosystem II, were unaffected. This study underscores the multifaceted implications of PMF contamination on the structure and function of periphyton in stream ecosystems.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"4 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demographic responses of an extremophile crustacean to environmental factors: Great Salt Lake (Utah, USA) brine shrimp (Artemia franciscana) 嗜极甲壳动物对环境因素的繁殖反应：大盐湖（美国犹他州）卤虾（Artemia franciscana）

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-06 DOI: 10.1007/s10750-024-05684-2

Gary E. Belovsky, Chad A. Larson, Heidi K. Mahon, Chad Mellison, Andrea C. Stumpf, Anghy Ramos Valencia

{"title":"Demographic responses of an extremophile crustacean to environmental factors: Great Salt Lake (Utah, USA) brine shrimp (Artemia franciscana)","authors":"Gary E. Belovsky, Chad A. Larson, Heidi K. Mahon, Chad Mellison, Andrea C. Stumpf, Anghy Ramos Valencia","doi":"10.1007/s10750-024-05684-2","DOIUrl":"https://doi.org/10.1007/s10750-024-05684-2","url":null,"abstract":"Hypersaline Great Salt Lake’s (GSL: Utah, USA) pelagic food web is dominated by the herbivore, Artemia franciscana. Artemia demographic responses (survival, developmental transition, and reproduction) to GSL salinities, temperatures, common phytoplankton and yeast, and food levels were examined by factorial experiment. Survival across developmental stages was best at 90 ppt salinity, and decreased as temperature increased. Transition between life stages was best at 45 ppt salinity, and increased as temperature increased. Food was most important with both survival and transitioning responding similarly to food types and increasing with amount of food. Artemia reproduce in two ways (diapausing cysts – oviparity, live young – ovoviviparity): ovoviviparous and total reproduction were greatest at 90 ppt salinity and 20 °C, while oviparous reproduction was weakly affected by salinity and greatest at 20 °C. Oviparity was greatest at low food availability, while ovoviviparity and total reproduction increased with food availability, so reproduction shifted from oviparity to ovoviviparity as food increased. Maternal effects were observed for cyst hatchability, and ovoviviparous nauplii survival and transitioning to the juvenile stage. Combinations of salinity, temperature, food taxa and food amount strongly affect demography, making single factor studies of limited value. Results explain Artemia abundance in different parts of GSL and among years.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"85 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0