Hydrobiologia最新文献

Whole-stream wastewater addition stimulates the green food web pathway but does not affect food chain length. 全流废水的添加刺激了绿色食物网通路，但不影响食物链长度。

IF 2.5 3区生物学

Hydrobiologia Pub Date : 2026-01-01 Epub Date: 2026-01-22 DOI: 10.1007/s10750-025-06103-w

Ioar de Guzman, Mario Brauns, Arturo Elosegi, Daniel von Schiller, Jose M González, José M Montoya, Aitor Larrañaga

{"title":"Whole-stream wastewater addition stimulates the green food web pathway but does not affect food chain length.","authors":"Ioar de Guzman, Mario Brauns, Arturo Elosegi, Daniel von Schiller, Jose M González, José M Montoya, Aitor Larrañaga","doi":"10.1007/s10750-025-06103-w","DOIUrl":"https://doi.org/10.1007/s10750-025-06103-w","url":null,"abstract":"Most rivers in developed regions receive tertiary-treated effluents from wastewater treatment plants that still contain a complex mixture of pollutants, but their combined effects on food webs are poorly understood. We had the unique opportunity to divert treated effluent into a previously pristine stream and conduct a before-after/control-impact/paired experiment to quantify effluent effects on green and brown food web pathways (i.e., algae and detritus-based pathways) and total food chain length. We hypothesized that the increased biofilm biomass after the effluent addition would promote its consumption by primary consumers, thus favoring the green pathway. Moreover, we hypothesized that the increase in the primary production would lead to an increase in food chain length. Coarse detritus remained the main food resource for primary consumers, but the addition of the effluent promoted biofilm biomass and increased the relative importance of the green food web pathway. However, the effluent discharge did not impact food chain length. The present study reveals that properly treated and substantially diluted effluents can still affect food web pathways through changes in dietary composition of consumers. Thus, further reducing nutrient inputs from WWTPs would minimize the impacts of effluents on food webs.Supplementary information: The online version contains supplementary material available at 10.1007/s10750-025-06103-w.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"853 9","pages":"2681-2694"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13035594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147591857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of lateral hydrological connectivity on the relative abundance of water frogs (Pelophylax spp.) and common toads (Bufo bufo) using eDNA surveys. 横向水文连通性对水蛙（Pelophylax spp.）和普通蟾蜍（Bufo Bufo）相对丰度的影响

IF 2.5 3区生物学

Hydrobiologia Pub Date : 2026-01-01 Epub Date: 2026-01-29 DOI: 10.1007/s10750-026-06110-5

Boglárka Mészáros, Andrea Funk, Thomas Hein, Lukas Landler, Paul Meulenbroek, Didier Pont, Alice Valentini, Dénes Schmera, István Czeglédi, Tibor Erős

{"title":"Effects of lateral hydrological connectivity on the relative abundance of water frogs (Pelophylax spp.) and common toads (Bufo bufo) using eDNA surveys.","authors":"Boglárka Mészáros, Andrea Funk, Thomas Hein, Lukas Landler, Paul Meulenbroek, Didier Pont, Alice Valentini, Dénes Schmera, István Czeglédi, Tibor Erős","doi":"10.1007/s10750-026-06110-5","DOIUrl":"https://doi.org/10.1007/s10750-026-06110-5","url":null,"abstract":"Riverine floodplains support diverse amphibian communities by providing essential habitats, with lateral hydrological connectivity (LHC or hydrology) determining local environmental conditions. Our study evaluated the direct and indirect effects of LHC on the two most abundant species in a riverine floodplain. We assessed the relative abundance of water frogs (Pelophylax spp.) and common toads (Bufo bufo) using eDNA surveys at 30 sites along an LHC gradient in the Danube River floodplain. We examined the habitat structure, vegetation cover, and the physical and chemical characteristics of waterbodies. Structural equation models (SEM) were used to explore LHC's direct and indirect effects, mediated by these environmental factors. Results showed that LHC did not directly influence Pelophylax spp. and B. bufo abundance but affected them indirectly and species specifically through environmental drivers. Hydrology negatively and indirectly impacted Pelophylax spp. through vegetation cover and the physical and chemical properties of waterbodies. B. bufo was affected in a similar way but primarily through habitat structure. Our findings highlight the importance of understanding species-specific responses to lateral hydrological connectivity in dynamic environments, as river regulation altering LHC can differentially impact amphibian species in riverine floodplains.Supplementary information: The online version contains supplementary material available at 10.1007/s10750-026-06110-5.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"853 9","pages":"2751-2767"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13098841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic and functional responses of benthic and drifting macroinvertebrates to fine sediment deposition: evidence from an alpine flume-based experiment. 底栖动物和漂流大型无脊椎动物对细沉积物沉积的分类和功能响应：来自高山水槽实验的证据。

IF 2.5 3区生物学

Hydrobiologia Pub Date : 2026-01-01 Epub Date: 2025-11-13 DOI: 10.1007/s10750-025-06014-w

Alberto Doretto, Andrea Faiola, Serena Masserano, Paola Rossi, Matteo Sassone, Matteo Zanarotto, Camilla Zucchi, Paul J Wood, Kate L Mathers

{"title":"Taxonomic and functional responses of benthic and drifting macroinvertebrates to fine sediment deposition: evidence from an alpine flume-based experiment.","authors":"Alberto Doretto, Andrea Faiola, Serena Masserano, Paola Rossi, Matteo Sassone, Matteo Zanarotto, Camilla Zucchi, Paul J Wood, Kate L Mathers","doi":"10.1007/s10750-025-06014-w","DOIUrl":"10.1007/s10750-025-06014-w","url":null,"abstract":"Excessive fine sediment deposition is a global pervasive issue in rivers and, in particular, for benthic organisms including macroinvertebrates. However, the assessment of fine sediment effects on the taxonomic and functional diversity of macroinvertebrate communities is challenging because of context-dependent confounding factors, such as river-flow conditions, land-use change, and anthropogenic pressures. By using a set of outdoor artificial flumes, this study examined the immediate (24-48 h) and two weeks post-addition response of Alpine stream macroinvertebrates to experimental deposition of fine sediment (7.5 kg m2). The taxonomic composition of benthic macroinvertebrate communities demonstrated significant differences between control and sediment-affected flumes; while taxonomic composition of drifting macroinvertebrates varied over time, with higher taxonomic heterogeneity for sedimented communities than control flumes. Nestedness was consistently the dominant component that contributed to beta-diversity patterns for both benthic and drifting invertebrates. No differences in taxonomic and functional community metrics were observed between control and sedimented communities, with the exception of a significant reduction in the total abundance of benthic macroinvertebrates in sedimented flumes. This study is one of the first flume-based experiments dealing with the response of Alpine stream macroinvertebrates to fine sediment deposition, and the findings highlight the context specific nature of fine sediment effects.Supplementary information: The online version contains supplementary material available at 10.1007/s10750-025-06014-w.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"853 5","pages":"1527-1546"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12855233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-frequency diatom dynamics seen in an ice- and snow-covered temperate lake using an imaging-in-flow cytometer. 用流式细胞仪成像在冰雪覆盖的温带湖泊中观察到的高频硅藻动力学。

IF 2.2 3区生物学

Hydrobiologia Pub Date : 2025-01-01 Epub Date: 2025-02-06 DOI: 10.1007/s10750-025-05802-8

Tara Tapics, Irene Gregory-Eaves, Yannick Huot

{"title":"High-frequency diatom dynamics seen in an ice- and snow-covered temperate lake using an imaging-in-flow cytometer.","authors":"Tara Tapics, Irene Gregory-Eaves, Yannick Huot","doi":"10.1007/s10750-025-05802-8","DOIUrl":"https://doi.org/10.1007/s10750-025-05802-8","url":null,"abstract":"The study of winter in temperate, ice-covered lakes has largely been neglected, creating a major gap in our understanding of annual phytoplankton cycles. We assessed patterns in biovolumes of Asterionella, cf. Synedra, Urosolenia, and total phytoplankton at three depths during the winter of 2014/2015 as estimated using images from an Imaging FlowCytobot (IFCB) moored in Lac (Lake) Montjoie, Quebec (Canada). Even though the use of the IFCB under-ice presented challenges, these were outstripped by its advantages. The IFCB provided unprecedented high temporal and spatial resolution phytoplankton count and biovolume data that allowed patterns of abundance to be observed in detail. Interestingly, the IFCB captured an abundance of Urosolenia, which may be dissolved in standard Lugol's-preserved grab samples. We found Asterionella grew until mid-December and then slowly decreased towards the spring, whereas cf. Synedra diatoms decreased from early December and then rebounded towards late December as light increased before falling again towards the spring. Urosolenia peaked in late December as cf. Synedra diatoms rebounded and then began to decline towards the spring. Total phytoplankton declined towards late December, increased in the beginning of January and then declined towards the spring. The studied diatoms maintained considerable under-ice seed populations and grew when light was sufficient.Supplementary information: The online version contains supplementary material available at 10.1007/s10750-025-05802-8.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"852 11","pages":"2887-2905"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the feeding of jellyfish polyps in wild and laboratory conditions: do experiments provide realistic estimates of natural functional rates? 对野生和实验室条件下水母珊瑚虫摄食的洞察：实验是否提供了自然功能率的现实估计？

IF 2.5 3区生物学

Hydrobiologia Pub Date : 2025-01-01 Epub Date: 2025-03-14 DOI: 10.1007/s10750-024-05783-0

Cathy H Lucas, Danja P Höhn, Clive N Trueman

{"title":"Insights into the feeding of jellyfish polyps in wild and laboratory conditions: do experiments provide realistic estimates of natural functional rates?","authors":"Cathy H Lucas, Danja P Höhn, Clive N Trueman","doi":"10.1007/s10750-024-05783-0","DOIUrl":"10.1007/s10750-024-05783-0","url":null,"abstract":"Biotic and abiotic factors that affect the physiology and ecology of scyphozoan polyps are considered to be major drivers of jellyfish blooms, but are rarely studied under field conditions. Here, stable isotopes of carbon and nitrogen were used to investigate feeding ecology in Aurelia aurita polyps from the Beaulieu River, UK (50° 80' 04.55″ N/1° 42' 28.12″ W) in both winter and summer conditions, and compared to laboratory-maintained polyps fed Artemia nauplii at 6 and 20 °C, respectively. In natural conditions, the isotopic composition of A. aurita polyps indicated assimilation of nutrients derived from both benthic and pelagic food pathways, with seasonal switches between benthic-derived nutrients in winter and pelagic-derived nutrients in summer. In laboratory experiments, polyps assimilated Artemia food at 6 °C although metabolic processes were reduced, while at 20 °C, polyps starved as their increased metabolic costs could not be met from the Artemia food. Experiments on growth and asexual reproduction of Artemia-fed polyps of A. aurita may not reflect natural metabolic rates especially at higher temperatures (e.g. 20 °C), because these polyps are not extracting sufficient resources from their Artemia food to fuel the increased metabolic costs associated with high temperatures.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"852 21","pages":"5249-5259"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12568851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145408882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ estimation of phytoplankton community growth rate inside dialysis membrane bags: a bioassay experiment at a fish farm in the eastern Aegean Sea 透析膜袋内浮游植物群落生长率的现场估算：东爱琴海养鱼场的生物测定实验

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05643-x

Betül Bardakcı Şener, Eyüp Mümtaz Tıraşın

{"title":"In situ estimation of phytoplankton community growth rate inside dialysis membrane bags: a bioassay experiment at a fish farm in the eastern Aegean Sea","authors":"Betül Bardakcı Şener, Eyüp Mümtaz Tıraşın","doi":"10.1007/s10750-024-05643-x","DOIUrl":"https://doi.org/10.1007/s10750-024-05643-x","url":null,"abstract":"We conducted a study to investigate the potential effects of nutrients released from a fish farm, which fell within the typical range found in oligotrophic offshore waters of the Aegean Sea, on phytoplankton growth. We designed an in situ bioassay experiment at a fish farm and incubated natural phytoplankton assemblages inside dialysis membrane bags for six days. Changes in phytoplankton growth in samples of ambient seawater collected throughout the experiment served as controls and were considered indicative of the net population change rates. Half of the bags were filled with seawater filtered through a 150-µm mesh, while the other half contained unfiltered seawater. The growth rates, estimated based on chlorophyll a concentrations and phytoplankton cell numbers inside the filtered and unfiltered bags, showed no significant differences. While no detectable net phytoplankton growth occurred in the ambient seawater, there was an exponential increase in chlorophyll a content and cell numbers within the bags. Moreover, the species richness within the bags gradually declined throughout the experiment. The findings of the study confirm that continuous nutrient releases from fish farms can promote high population growth rates in oligotrophic environments, provided that phytoplankton losses due to grazing, advection, and sinking are minimized or eliminated.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"20 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whistle structure variation between two sympatric dolphin species in the Gulf of California 加利福尼亚湾两种同域海豚的哨音结构变异

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05675-3

Simone Antichi, Manali Rege‑Colt, Maia Austin, Laura J. May‑Collado, Óscar Carlón-Beltrán, Jorge Urbán R., Sergio Martínez-Aguilar, Lorena Viloria-Gómora

{"title":"Whistle structure variation between two sympatric dolphin species in the Gulf of California","authors":"Simone Antichi, Manali Rege‑Colt, Maia Austin, Laura J. May‑Collado, Óscar Carlón-Beltrán, Jorge Urbán R., Sergio Martínez-Aguilar, Lorena Viloria-Gómora","doi":"10.1007/s10750-024-05675-3","DOIUrl":"https://doi.org/10.1007/s10750-024-05675-3","url":null,"abstract":"Dolphins produce narrowband and frequency modulated sounds called whistles during a variety of behavioral contexts. Dolphin species vary in their whistle contour composition, frequency range, modulation, and duration, and these differences can be useful in their identification. Here, we compare the whistle contours of two sympatric dolphin species at La Paz Bay, Gulf of California, the Eastern Tropical Pacific bottlenose dolphin, and the long-beaked common dolphin. Dolphins were recorded (7 h and 1 min recording effort) from the research vessel with the engine off using an over-the-side hydrophone and a broadband recording system. A total of 666 high quality whistles (bottlenose dolphin, n = 415; long-beaked common dolphin, n = 251) were analyzed. A Random Forest Analysis identified duration, ending frequency, and maximum frequency as the most important variables that distinguish the two dolphin species. The most common whistle contour types of bottlenose dolphins were sine and convex, while the long-beaked common dolphins produced mainly upsweep and concave whistles. The results showed that bottlenose dolphins and long-beaked common dolphins differ in their whistle variables and contours facilitating their identification in future passive acoustic studies.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"16 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitology of one of the world’s foremost fisheries target species lacks a One Health approach 世界上最重要的渔业目标物种之一的寄生虫学缺乏 "一体健康 "方法

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-16 DOI: 10.1007/s10750-024-05695-z

Miriam Isoyi Shigoley, Nicolas Antoine-Moussiaux, Thierry Jauniaux, Maarten P. M. Vanhove

{"title":"Parasitology of one of the world’s foremost fisheries target species lacks a One Health approach","authors":"Miriam Isoyi Shigoley, Nicolas Antoine-Moussiaux, Thierry Jauniaux, Maarten P. M. Vanhove","doi":"10.1007/s10750-024-05695-z","DOIUrl":"https://doi.org/10.1007/s10750-024-05695-z","url":null,"abstract":"The global demand for affordable animal protein, particularly Nile tilapia, has driven increased adoption of (semi-)intensive farming practices. This intensification poses challenges like fish disease outbreaks, higher parasite loads, increased mortality rates, and environmental degradation. Addressing these issues requires a comprehensive understanding of the biology and ecology of these disrupted equilibria, emphasizing the need to characterize parasites, their pathogenic effects, and the conditions facilitating their emergence. Despite 276 known parasite species infecting Nile tilapia, existing reports are fragmented, often conducted locally or focused on a few species in experimental settings. A timely challenge is summarizing the state of knowledge and presenting links between human, animal, and environmental health. Unfortunately, limited studies focus on these parasites’ actual effects and environmental correlates, indicating little research effort. Comparing the number of parasite species described with few studies documenting their impacts reveals large knowledge gaps. The current information on these parasites lacks practical applicability for stakeholders in production and management. Bridging this knowledge gap requires both descriptive and experimental studies. Adopting the One Health approach in parasitological assessments and conducting further research will ensure aquaculture stakeholders can access valuable information for informed decision-making, prioritizing environmental integrity, fish health and welfare, and consumer well-being.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"189 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing agricultural land use in riparian networks negatively affects stream fish communities in a tropical savanna 河岸网络中农业用地的增加对热带稀树草原的溪流鱼类群落产生负面影响

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-13 DOI: 10.1007/s10750-024-05697-x

Jenny J. Morales, Lúcia Mateus, Luiza Peluso, Peter Zeilhofer, Bruno R. S. Figueiredo, Jerry Penha

{"title":"Increasing agricultural land use in riparian networks negatively affects stream fish communities in a tropical savanna","authors":"Jenny J. Morales, Lúcia Mateus, Luiza Peluso, Peter Zeilhofer, Bruno R. S. Figueiredo, Jerry Penha","doi":"10.1007/s10750-024-05697-x","DOIUrl":"https://doi.org/10.1007/s10750-024-05697-x","url":null,"abstract":"The Brazilian Cerrado has been experiencing intense land use changes for decades, with large-scale conversion of its natural vegetation to agriculture and livestock grazing. These agents of landscape change also affect the aquatic ecosystems embedded in the biome. Here, we investigated the effects of land use at different scales (catchment, riparian network, and local riparian) on fish assemblages in streams of the Brazilian Cerrado. We hypothesized that increasing agricultural land use (ALU%) reduces fish diversity and the compositional uniqueness of the fish assemblage. We used GLM to evaluate the impact of low-intensity ALU% on fish richness and abundance. Additionally, we employed beta regression to analyze the effect on local contribution to beta diversity (LCBD) and redundancy analysis to assess the impact on species composition. Our results showed that the effects of low-intensity ALU% depend on the spatial scale. While increasing ALU% in the riparian network negatively affected LCBD and altered species composition, ALU% in the catchment had negligible to slightly positive effects on LCDB, within the range of ALU% considered. Our findings reinforce the need to preserve riparian forests to maintain beta diversity in the streams of the Brazilian Cerrado. Also, no evidence was found that low-intense catchment use, without degrading the riparian forest, affects fish assemblages.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"11 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of phytoplankton diversity using microscopy and metabarcoding: insights from an eLTER station in the Northern Adriatic Sea 利用显微镜和代谢编码对浮游植物多样性进行比较分析：北亚得里亚海 eLTER 站的启示

IF 2.6 3区生物学

Hydrobiologia Pub Date : 2024-09-12 DOI: 10.1007/s10750-024-05692-2

Francesca Neri, Marika Ubaldi, Stefano Accoroni, Sara Ricci, Elisa Banchi, Tiziana Romagnoli, Cecilia Totti

{"title":"Comparative analysis of phytoplankton diversity using microscopy and metabarcoding: insights from an eLTER station in the Northern Adriatic Sea","authors":"Francesca Neri, Marika Ubaldi, Stefano Accoroni, Sara Ricci, Elisa Banchi, Tiziana Romagnoli, Cecilia Totti","doi":"10.1007/s10750-024-05692-2","DOIUrl":"https://doi.org/10.1007/s10750-024-05692-2","url":null,"abstract":"The monitoring of phytoplankton is crucial to highlight changes in the marine ecosystems. In the present study, the phytoplankton community of an eLTER station in the Northern Adriatic Sea was analysed combining two approaches, i.e. microscopy and eDNA metabarcoding (targeting V4 and V9 regions of the 18S rRNA gene, and using PR2 and SILVA as reference databases), to highlight the strengths and weaknesses of these two methods. Metabarcoding revealed a so far unknown phytoplankton diversity (99 genera and 151 species), while microscopy detected 14 genera and 44 species not revealed by metabarcoding. Only a small percentage of genera and species were shared by the two methods (microscopy and metabarcoding), 18S regions (V4 and V9) and reference databases (PR2 and SILVA). Metabarcoding showed a community characterized by a higher number of phytoflagellate and dinoflagellate genera and species, in comparison with microscopy where diatom and dinoflagellate taxa were the most represented. Moreover, metabarcoding failed to reveal almost all the coccolithophores. The results confirmed metabarcoding as a powerful tool, but it should still be combined with microscopy to have a more detailed information on the community and to counteract the drawbacks of metabarcoding, such as gaps in the reference databases.","PeriodicalId":13147,"journal":{"name":"Hydrobiologia","volume":"7 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0