HAYATI Journal of Biosciences最新文献_第5页

Development of Few Significant SNP Markers from Transcriptomic Data for Selection of Sengon (Falcataria falcata (L.) Greuter & R. Rankin) Resistant to Boktor Stem Borer and Gall Rust Disease 从转录组学数据中开发几个重要的SNP标记用于镰形镰刀(Sengon Falcataria falcata)的选择Greuter,抗Boktor茎螟虫和瘿锈病

HAYATI Journal of Biosciences Pub Date : 2023-10-16 DOI: 10.4308/hjb.31.1.110-125

Aditya Nugroho, Vilda Puji Dini Anita, Deden Derajat Matra, Iskandar Zulkarnaen Siregar, Ulfah Juniarti Siregar

{"title":"Development of Few Significant SNP Markers from Transcriptomic Data for Selection of Sengon (Falcataria falcata (L.) Greuter & R. Rankin) Resistant to Boktor Stem Borer and Gall Rust Disease","authors":"Aditya Nugroho, Vilda Puji Dini Anita, Deden Derajat Matra, Iskandar Zulkarnaen Siregar, Ulfah Juniarti Siregar","doi":"10.4308/hjb.31.1.110-125","DOIUrl":"https://doi.org/10.4308/hjb.31.1.110-125","url":null,"abstract":"Sengon (Falcataria falcata (L.) Greuter & R. Rankin) plantations in Indonesia are threatened by attacks from Boktor stem borers and gall rust disease. Controlling pests and diseases is difficult; therefore, planting resistant trees obtained from tree selection programs is necessary. Currently, genomic breeding often incorporates GWAS, which uses thousands of SNP markers to identify markers with significant associations with the traits studied. This study aimed to bypass such expensive studies by identifying and developing SNP markers from sequences of putative resistance genes to Boktor stem borer and gall rust disease, identified from sengon transcriptomic data analysis. A total of 496,194 putative SNP sites were identified from transcriptomic sequences using the SAMtools and BFCtools programs, of which 119 SNP sites were associated with resistance genes. Of the 101 non-synonymous SNPs selected, only 12 were located in the conserved domain of each gene and were used for primer design. Of the 13 primers designed, only 10 were successfully amplified. Validation of 10 developed SNP markers on 100 sengon accessions using the HRM method confirmed a significant association between SNP markers and resistance traits, with a -log 10 (P-value) between 10.49 and 16.63. A few SNPs markers developed from putative resistance gene sequences are associated with resistance traits in sengon. Therefore, the SNP markers could be applied in selection programs for sengon trees resistant to Boktor stem borers and gall rust disease.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Genetic Variability of Indonesian Local Foxtail Millet Accession Based on Agro-morphological Traits and Early Salinity Tolerance Evaluation Utilizing SiDREB2-based SNAP Marker 印尼地方谷子形态性状遗传变异及基于sidreb2的SNAP标记早期耐盐性评价

HAYATI Journal of Biosciences Pub Date : 2023-10-12 DOI: 10.4308/hjb.31.1.82-93

Sri Ratnawati, Rika Miftakhul Jannah, Yuli Irvana Dewi, Ramadaniarto Rizqullah, Willy Bayuardi Suwarno, Sintho Wahyuning Ardie

{"title":"The Genetic Variability of Indonesian Local Foxtail Millet Accession Based on Agro-morphological Traits and Early Salinity Tolerance Evaluation Utilizing SiDREB2-based SNAP Marker","authors":"Sri Ratnawati, Rika Miftakhul Jannah, Yuli Irvana Dewi, Ramadaniarto Rizqullah, Willy Bayuardi Suwarno, Sintho Wahyuning Ardie","doi":"10.4308/hjb.31.1.82-93","DOIUrl":"https://doi.org/10.4308/hjb.31.1.82-93","url":null,"abstract":"Foxtail millet (Setaria italica L. Beauv.) is a grain-producing crop with high nutritional benefits and adaptability to broad environmental conditions. Indonesian local foxtail millet accessions are valuable genetic resources for crop improvement of the species. This study aims to assess the biodiversity of Indonesian local foxtail millet accessions and estimate their salinity tolerance level utilizing the SiDREB2-based SNAP marker and early evaluation at the seedling stage. Our results showed that eight Indonesian local foxtail millet accessions, namely Toraja, Mauliru-2, Hambapraing, NTB-1, ICERI-5, ICERI-6, Botok-4, and Botok-10, had high variability based on agro-morphological traits. The Hambapraing and Mauliru-2 were the potential accessions with high yield estimates (2.33 ton.ha-1 and 1.93 ton.ha-1) and early harvest time (<110 DAT). ICERI-6 was indicated as a salinity-tolerant genotype based on the SiDREB2-based marker and early salinity evaluation at the seedling stage, while Toraja, Mauliru-2, NTB-1, and Botok-4 were categorized as sensitive genotypes. Germplasm variability and the estimated salinity tolerance level of Indonesian local foxtail millet accessions presented in this study serve as essential information in the foxtail millet breeding for high productivity, early harvest time, and salinity tolerant variety.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136057908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Averrhoa bilimbi Leaf Extract on Blood Glucose Level, Hepatosomatic Index (HSI), and Liver Histology of Diabetic Mice 牛膝叶提取物对糖尿病小鼠血糖水平、肝体指数及肝脏组织学的影响

HAYATI Journal of Biosciences Pub Date : 2023-10-12 DOI: 10.4308/hjb.31.1.102-109

Alan Verangga, Nur Qomariyah, Firas Khaleyla

{"title":"Effect of Averrhoa bilimbi Leaf Extract on Blood Glucose Level, Hepatosomatic Index (HSI), and Liver Histology of Diabetic Mice","authors":"Alan Verangga, Nur Qomariyah, Firas Khaleyla","doi":"10.4308/hjb.31.1.102-109","DOIUrl":"https://doi.org/10.4308/hjb.31.1.102-109","url":null,"abstract":"Averrhoa bilimbi leaf extract (BLE) contains high antioxidant levels. Antioxidants can suppress reactive oxygen species produced during hyperglycemic conditions in diabetes mellitus (DM), which cause damage to liver tissue. This study aimed to determine the BLE effect on fasting blood glucose (FBG), Hepatosomatic Index (HSI), and liver tissue damage. We used 24 mice in 6 different groups, divided by N (normal), K- (DM), K+ (DM+glibenclamide 0.013mg/20gBW), E1 (DM+ extract 6.3mg/20gBW), E2 (DM+ extract 8.4mg/20gBW), and E3 (DM + extract 10.5mg/20gBW). We used an alloxan dose of 120mg/kgBW to induce the DM and then treated with BLE for 14 days. We measured the fasting blood glucose using a glucometer. In addition, we evaluated the liver tissue damage with HSI and Hematoxylin-Eosin (HE) stained histological slides. The results showed that BLE significantly reduced the percentage of FBG and liver tissue damage, while HSI showed no significant difference. The most optimal extract dose was 8.4 mg/20gBW (E2 group), with an FBG decrease of 26.44%, a normal cell percentage of 88.56%, and an HSI score of 6.18%. Based on this finding, we concluded that bilimbi leaf extract could lower blood glucose and improve liver histology of diabetic mice but did not significantly affect HSI.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136057910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous Zinc Application and Generative Traits of Three Local Shallot Varieties 三个地方大葱品种外源锌的施用及生殖性状研究

HAYATI Journal of Biosciences Pub Date : 2023-10-12 DOI: 10.4308/hjb.31.1.94-101

Remi Widana Putri, Elkawakib Syam'un, Fachirah Ulfa

{"title":"Exogenous Zinc Application and Generative Traits of Three Local Shallot Varieties","authors":"Remi Widana Putri, Elkawakib Syam'un, Fachirah Ulfa","doi":"10.4308/hjb.31.1.94-101","DOIUrl":"https://doi.org/10.4308/hjb.31.1.94-101","url":null,"abstract":"Increasing shallot (Allium ascalonicum L.) production can be done by application of botanical or true shallot seeds (TSS). Meanwhile, it is well understood that botanical seeds are difficult to produce due to shallots' low flowering capacity. This study aimed to evaluate the generative traits of three local shallot varieties affected by various doses of zinc (Zn). This study was structured using a split-plot design, where the main plots were varieties (Lokana, Rubaru, and Ambassador 3 Agrihorti). In addition, the sub-plot treatment, the dose of zinc (0, 0.5, 1, and 1.5 kg ha-1), was repeated three times. There is an effect of the main factor (variety) where Rubaru and Ambassador 3 Agrihorti show the fastest umbel emergence. In addition, our data show the interaction effects on the age of sheat breaking, flower blooming (DAP), morphology traits of generative organs, and leaf traits. In detail, Lokana with a Zn dose of 1.5 ha-1 shows the best results on the length and diameter of the umbel stalk; Rubaru with Zn 1 kg ha-1 on the age of broken sheath and chlorophyll index; and the Ambassador 3 Agrihorti with Zn 0.5 kg ha-1 on the number of flowers. On the one hand, it can be concluded that each variety responded differently to the dose of Zn. Concerning seed production, on the other hand, the Ambassador 3 Agrihorti with Zn 0.5 kg ha-1 has better potential to be developed for TSS, as seen from the number of flowers per umbel, a higher percentage of flowering plants and a relatively fast flowering time than other varieties.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136057907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less: Leaf, Stem, and Root 梅花的显微评价及薄层色谱分析少:叶，茎和根

HAYATI Journal of Biosciences Pub Date : 2023-10-11 DOI: 10.4308/hjb.31.1.71-81

Ni Putu Ermi Hikmawanti, Fadlina Chany Saputri, Arry Yanuar, Ratih Asmana Ningrum, Abdul Mun'im, Hayati Hayati

{"title":"Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less: Leaf, Stem, and Root","authors":"Ni Putu Ermi Hikmawanti, Fadlina Chany Saputri, Arry Yanuar, Ratih Asmana Ningrum, Abdul Mun'im, Hayati Hayati","doi":"10.4308/hjb.31.1.71-81","DOIUrl":"https://doi.org/10.4308/hjb.31.1.71-81","url":null,"abstract":"Pluchea indica (L.) Less is traditionally utilized to treat postpartum women in Indonesia. The plant has many pharmacological properties, so that it can be further developed as herbal medicine. In that development process, plant authentication is needed to ensure the quality of raw materials. A simple microscopical and thin-layer chromatography (TLC) analysis might be a way to authenticate the plant, but it has never been reported. So, this study evaluates the microscopical and TLC analysis for P. indica authentication in standardized herbal medicines production. Plant microscopic observation, fluorescence analysis, and polyphenol screening were conducted. n-Hexane, ethyl acetate, and methanol extracts of plant organs were then analyzed by TLC. Here, we reported that in microscopical analysis the simplicia of P. indica contains trichomes and tannin-containing cells. In addition, chlorogenic acid as a marker was present in TLC analysis by ethyl acetate-water-formic acid-acetic acid (8.5:1.5:1:1, v/v). The results of this evaluation might provide additional information in the identification, authentication, and quality control of P. indica as a raw material for herbal medicine.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136253882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidative Parameters Improvements on Nutritional Approach: A Study on Hypoxic Multiple Organs of Sprague-Dawley 营养方法改善抗氧化参数:Sprague-Dawley多器官缺氧的研究

HAYATI Journal of Biosciences Pub Date : 2023-10-09 DOI: 10.4308/hjb.31.1.59-70

Andreanyta Meliala, Paramita Narwidina, Yogi Tri Sumarno

{"title":"Antioxidative Parameters Improvements on Nutritional Approach: A Study on Hypoxic Multiple Organs of Sprague-Dawley","authors":"Andreanyta Meliala, Paramita Narwidina, Yogi Tri Sumarno","doi":"10.4308/hjb.31.1.59-70","DOIUrl":"https://doi.org/10.4308/hjb.31.1.59-70","url":null,"abstract":"In various organs, such as the heart, kidneys, and colon, hypoxia enhances the generation of reactive oxygen species (ROS). However, the effects of reoxygenation, as occurs in intermittent hypoxia (IH) to achieve full recovery of hypoxic organs, are not yet clear. The acclimatization response can boost blood oxygen transport capacity, while hypoxia ROS can impact erythrocytes and plasma behavior, resulting in poor peripheral blood flow. This study aimed to study the antioxidant impact of puree Ficus carica (PFC) in rats with IH-induced oxidative stress. Twenty-nine Sprague-Dawley rats were randomly divided into five groups: group N (control, untreated and not exposed to IH, and Group HC was exposed to hypoxia and received distilled water. Group HPF-6.25, HPF-12.5, and HPF-25 (n = 6) received PFC with doses 6.25; 12.5; and 25 ml/kg/d, respectively for 4 weeks before IH exposure. At the end of 4 weeks, all animals except controls were exposed to IH (10% O2 and 90% N2; 4 hours/day for one week). Hematological parameters were measured with several oxidative stress indicators. Hypoxic rats exhibited substantially higher hemoglobin, hematocrit, and mean corpuscular hemoglobin concentrations. All groups exposed to IH showed increased malondialdehyde (MDA) and decreased superoxide dismutase (SOD) activity in the heart, kidneys, and colon. The increasing MDA and decreasing SOD compared to controls and pre-treatment using PFC had a dose-dependent protective effect on the heart, kidneys, and colon.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"296 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135196058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomineralization Biotechnology Utilizing Lysinibacillus sphaericus to Improve Mechanical Properties of Mortar 利用球形赖氨酸芽孢杆菌改善砂浆力学性能的生物矿化技术

HAYATI Journal of Biosciences Pub Date : 2023-10-03 DOI: 10.4308/hjb.31.1.48-58

Ridwan Syarif, Siti Khodijah Chaerun, Ridho Kresna Wattimena

{"title":"Biomineralization Biotechnology Utilizing Lysinibacillus sphaericus to Improve Mechanical Properties of Mortar","authors":"Ridwan Syarif, Siti Khodijah Chaerun, Ridho Kresna Wattimena","doi":"10.4308/hjb.31.1.48-58","DOIUrl":"https://doi.org/10.4308/hjb.31.1.48-58","url":null,"abstract":"Biomineralization has notably enhanced the qualities of cement-based materials, particularly through bacterial-facilitated calcite precipitation via calcium lactate oxidation. However, existing research mainly targets self-healing aspects, with little focus on bio-based mortar properties. Consequently, this study provides a comprehensive examination of the enhancements in dry density, ultrasonic pulse velocity (UPV), and flexural strength, achieved through the application of a novel indigenous bacterial strain (Lysinibacillus sphaericus strain SKC/VA-1) from Indonesia, coupled with the incorporation of calcium lactate pentahydrate as an additive. A total of six mortar samples were prepared to investigate the influence of bacteria on the properties of mortar through biomineralization. The samples included plain mortar (M), mortar mixed with calcium lactate pentahydrate (ML), mortar mixed with a 10% v/v bacterial inoculum (MB1), mortar mixed with calcium lactate pentahydrate and a 10% v/v bacterial inoculum (MLB1), mortar mixed with a 20% v/v bacterial inoculum (MB2), and mortar mixed with calcium lactate pentahydrate and a 20% v/v bacterial inoculum (MLB2). The employment of a distinct bacterial strain for oxidizing calcium lactate represents an innovative aspect of the current study. The presence of organic calcium was found to have no adverse effects on the mortar matrix. Optimal inoculum concentrations of bacteria (10% v/v), in conjunction with calcium lactate pentahydrate, yielded superior mechanical properties. Mineralogical characterization via X-ray diffraction and microstructural analysis through scanning electron microscopy substantiated the incidence of calcite precipitation, which facilitated pore infilling and consequently augmented both the ultrasonic pulse velocity and the flexural strength of the mortar.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135788948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative Stress, Hypoxia-Inducible Factor-1α, and Nuclear Factor-Erythroid 2-Related Factor 2 in the Hearts of Rats Exposed to Intermittent Hypobaric Hypoxia 间歇性低氧大鼠心脏氧化应激、缺氧诱导因子-1α和核因子-红细胞2相关因子2的研究

HAYATI Journal of Biosciences Pub Date : 2023-09-25 DOI: 10.4308/hjb.31.1.39-47

Wardaya Wardaya, Wawan Mulyawan, Sri Widia A Jusman, Mohamad Sadikin

{"title":"Oxidative Stress, Hypoxia-Inducible Factor-1α, and Nuclear Factor-Erythroid 2-Related Factor 2 in the Hearts of Rats Exposed to Intermittent Hypobaric Hypoxia","authors":"Wardaya Wardaya, Wawan Mulyawan, Sri Widia A Jusman, Mohamad Sadikin","doi":"10.4308/hjb.31.1.39-47","DOIUrl":"https://doi.org/10.4308/hjb.31.1.39-47","url":null,"abstract":"Hypobaric hypoxia is situation that might occur to helicopter pilots in Indonesia who must fly at an altitude of more than 3,048 m such as in Papua. It can be dangerous because hypoxic condition can affect person's performance. So far, the heart is known as an aerobic organ and very sensitive to hypoxic conditions. Hitherto, the effects of hypobaric hypoxia exposure on biomolecular aspects of the heart are still unclear. Therefore, this study assessed cardiac response in rats exposed to intermittent hypobaric hypoxia (IHH) (equivalent to 3,048 meters/10,000 feet). Sprague-Dawley rats were divided into six groups: control; acute hypobaric hypoxia (AHH); and IHH, for 7; 14; 21; and 28 days. We measured super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH), malondialdehyde (MDA), cytoglobin, myoglobin, HIF-1α, and Nrf2 level as our parameters. Activities of SOD, CAT, GPx and GSH increased while the levels of MDA, cytoglobin, myoglobin, HIF-1α, and Nrf2 decreased in all IHH groups compared with the AHH group. A biphasic pattern was observed as IHH sessions increased from 14 to 21 or 28. Where the IHH treatment for more than 14 sessions caused a decrease in endogenous antioxidants, but the response to hypoxia and oxidative stress increased. Our findings presented the molecular alterations of cardiac rats exposed to intermittent hypobaric hypoxia.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135924915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosynthetic Rate Prediction Model of Golden Melon Plant (Cucumis melo L.) at Vegetative Phase in Greenhouse using Artificial Neural Networks 基于人工神经网络的温室金瓜植物营养期光合速率预测模型

HAYATI Journal of Biosciences Pub Date : 2023-09-22 DOI: 10.4308/hjb.31.1.30-38

None Erniati, Herry Suhardiyanto, Rokhani Hasbullah, None Supriyanto

{"title":"Photosynthetic Rate Prediction Model of Golden Melon Plant (Cucumis melo L.) at Vegetative Phase in Greenhouse using Artificial Neural Networks","authors":"None Erniati, Herry Suhardiyanto, Rokhani Hasbullah, None Supriyanto","doi":"10.4308/hjb.31.1.30-38","DOIUrl":"https://doi.org/10.4308/hjb.31.1.30-38","url":null,"abstract":"The most critical parameter affecting plant growth is the photosynthetic rate. The parameter can be determined by measuring the rate of CO2 assimilation that occurs in plants. Developing a photosynthetic rate model can recommend proper cultivation maintenance in melon plants. Hence, the involvement of input parameters in the developed model affects the accuracy of the prediction. This study aims to develop an artificial neural networks (ANNs) prediction model of the photosynthetic rate of melon plants in the vegetative phase in the greenhouse based on seven environmental and growth parameters and find the best model structure. Model development uses artificial neural networks with several stages: data collection and pre-processing, model development with different input variations, model validation, and selection of the best scenario to predict photosynthetic rate. The results showed that five out of seven input parameters, i.e., air temperature, sunlight intensity, CO2 concentration, air humidity, and plant rows, in the model structure of five inputs, six hidden and one output were the best model scenarios with coefficient of determination (R2) and root mean square error (RMSE) of 0.986 and 0.420, respectively.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136100432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics 滨海鸡血藤抗痛风活性研究。黄嘌呤氧化酶提取物:代谢谱及抑制动力学

HAYATI Journal of Biosciences Pub Date : 2023-09-19 DOI: 10.4308/hjb.31.1.1-20

Rut Novalia Rahmawati Sianipar, Komar Sutriah, Dyah Iswantini, None Trivadila, Suminar Setiati Achmadi

{"title":"Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics","authors":"Rut Novalia Rahmawati Sianipar, Komar Sutriah, Dyah Iswantini, None Trivadila, Suminar Setiati Achmadi","doi":"10.4308/hjb.31.1.1-20","DOIUrl":"https://doi.org/10.4308/hjb.31.1.1-20","url":null,"abstract":"The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0