牛膝叶提取物对糖尿病小鼠血糖水平、肝体指数及肝脏组织学的影响

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-10-12 DOI:10.4308/hjb.31.1.102-109

Alan Verangga, Nur Qomariyah, Firas Khaleyla

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引用次数: 0

摘要

牛蒡叶提取物(BLE)含有高抗氧化水平。抗氧化剂可以抑制糖尿病(DM)高血糖状态下产生的活性氧，从而导致肝组织损伤。本研究旨在确定BLE对空腹血糖(FBG)、肝体指数(HSI)和肝组织损伤的影响。选取24只小鼠，分为6组，按N(正常)、K- (DM)、K+ (DM+格列苯脲0.013mg/20gBW)、E1 (DM+提取物6.3mg/20gBW)、E2 (DM+提取物8.4mg/20gBW)、E3 (DM+提取物10.5mg/20gBW)进行分组。我们用120mg/kgBW的四氧嘧啶诱导DM，然后用BLE治疗14 d。我们用血糖仪测量空腹血糖。此外，我们用HSI和苏木精-伊红(HE)染色的组织学切片评估肝组织损伤。结果显示，BLE显著降低FBG百分比和肝组织损伤，而HSI无显著差异。最佳提取剂量为8.4 mg/20gBW (E2组)，FBG降低26.44%，正常细胞率为88.56%，HSI评分为6.18%。综上所述，我们认为枸杞叶提取物可以降低糖尿病小鼠的血糖，改善肝脏组织学，但对HSI没有显著影响。

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Effect of Averrhoa bilimbi Leaf Extract on Blood Glucose Level, Hepatosomatic Index (HSI), and Liver Histology of Diabetic Mice

Averrhoa bilimbi leaf extract (BLE) contains high antioxidant levels. Antioxidants can suppress reactive oxygen species produced during hyperglycemic conditions in diabetes mellitus (DM), which cause damage to liver tissue. This study aimed to determine the BLE effect on fasting blood glucose (FBG), Hepatosomatic Index (HSI), and liver tissue damage. We used 24 mice in 6 different groups, divided by N (normal), K- (DM), K+ (DM+glibenclamide 0.013mg/20gBW), E1 (DM+ extract 6.3mg/20gBW), E2 (DM+ extract 8.4mg/20gBW), and E3 (DM + extract 10.5mg/20gBW). We used an alloxan dose of 120mg/kgBW to induce the DM and then treated with BLE for 14 days. We measured the fasting blood glucose using a glucometer. In addition, we evaluated the liver tissue damage with HSI and Hematoxylin-Eosin (HE) stained histological slides. The results showed that BLE significantly reduced the percentage of FBG and liver tissue damage, while HSI showed no significant difference. The most optimal extract dose was 8.4 mg/20gBW (E2 group), with an FBG decrease of 26.44%, a normal cell percentage of 88.56%, and an HSI score of 6.18%. Based on this finding, we concluded that bilimbi leaf extract could lower blood glucose and improve liver histology of diabetic mice but did not significantly affect HSI.

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.