Hearing Research最新文献_第3页

Chirp sensitivity and vowel coding in the inferior colliculus 下丘的啁啾灵敏度和元音编码

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-14 DOI: 10.1016/j.heares.2025.109307

Paul W. Mitchell , Laurel H. Carney

{"title":"Chirp sensitivity and vowel coding in the inferior colliculus","authors":"Paul W. Mitchell , Laurel H. Carney","doi":"10.1016/j.heares.2025.109307","DOIUrl":"10.1016/j.heares.2025.109307","url":null,"abstract":"<div><div>The inferior colliculus (IC) is an important brain region to understand neural encoding of complex sounds due to its diverse sound-feature sensitivities, including features that are affected by peripheral nonlinearities. Recent physiological studies in rabbit IC demonstrate that IC neurons are sensitive to chirp direction and velocity. Fast spectrotemporal changes, known as chirps, are contained within pitch-periods of natural vowels. Here, we use a combination of physiological and modeling strategies to assess the impact of chirp-sensitivity on vowel coding. Neural responses to vowel stimuli were recorded and vowel-token identification was evaluated based on average-rate and spike-timing metrics. Response timing was found to result in higher identification accuracy than rate. Additionally, rate bias towards low-velocity chirps, independent of chirp direction, was shown to correlate with higher vowel-identification accuracy based on timing. Also, direction bias in response to chirps of high velocity was shown to correlate with vowel-identification accuracy based on both rate and timing. Responses to natural-vowel tokens of individual neurons were simulated using an IC model with controllable chirp sensitivity. Responses of upward-biased, downward-biased, and non-selective model neurons were generated. Manipulating chirp sensitivity influenced response profiles across natural vowel tokens and vowel discrimination based on model-neuron responses. More work is needed to match all features of model responses to those of physiological recordings.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109307"},"PeriodicalIF":2.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echolocation frequency alteration, and hearing loss induced by an ototoxic drug in the echolocating bat Hipposideros pratti 耳毒性药物对回声定位蝙蝠回声定位频率改变和听力损失的影响

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-12 DOI: 10.1016/j.heares.2025.109304

Jianwen Zou, Yalin Wang, Shuilian Yang, Yanjie Zhang, Qicai Chen, Ziying Fu

{"title":"Echolocation frequency alteration, and hearing loss induced by an ototoxic drug in the echolocating bat Hipposideros pratti","authors":"Jianwen Zou, Yalin Wang, Shuilian Yang, Yanjie Zhang, Qicai Chen, Ziying Fu","doi":"10.1016/j.heares.2025.109304","DOIUrl":"10.1016/j.heares.2025.109304","url":null,"abstract":"<div><div>Noise exposure increases the level of reactive oxygen species (ROS) in the outer hair cells (OHCs) of the cochlea, which subsequently damages OHCs and causes noise-induced hearing loss. However, increasing evidences have shown that echolocating bats can maintain their auditory sensitivity after intense noise exposure, indicating that they have a strong capacity to clear ROS, or that noise exposure does not increase ROS levels in the cochlea of the animals. To differentiate between these possibilities, the constant frequency-frequency modulation bats, <em>Hipposideros pratti</em>, were intraperitoneally injected with the ototoxic drug Kanamycin, which increases ROS levels in the cochlea of other mammalian species. The results showed that Kanamycin application efficiently altered the echolocation frequency, shifted the auditory brainstem response threshold, and damaged the OHCs, suggesting that echolocating bats were sensitive to ototoxic drugs. Therefore, <em>H. pratti</em> does not seem to be able to clear ROS efficiently, and the decreased susceptibility to noise exposure of echolocating bats might be due to the failed increasement of ROS levels in their cochleae by the noise exposure. Furthermore, our data also showed that the precision of resting frequency (RF) was greatly decreased after the auditory sensitivity was impaired by Kanamycin application, suggesting that the precision of the RF was dependent on the auditory feedback. These findings could provide insights to understand the adaptation mechanisms of the auditory system of echolocating bats to intense sound environments.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109304"},"PeriodicalIF":2.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of metabolomics to acquired hearing loss: Advances and systematic review 代谢组学在获得性听力损失中的应用：进展与系统综述

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-10 DOI: 10.1016/j.heares.2025.109302

Shuyi Wang , Mengyao Xie , Zhiyuan Wu , Shu Wang , Qi Tang , Chen Li , Zhijin Han , Yuan Cao , Yurun Chen , Hua Yang

{"title":"Application of metabolomics to acquired hearing loss: Advances and systematic review","authors":"Shuyi Wang , Mengyao Xie , Zhiyuan Wu , Shu Wang , Qi Tang , Chen Li , Zhijin Han , Yuan Cao , Yurun Chen , Hua Yang","doi":"10.1016/j.heares.2025.109302","DOIUrl":"10.1016/j.heares.2025.109302","url":null,"abstract":"<div><div>Hearing loss poses a significant global public health challenge, seriously jeopardizing both physical and mental health. As an emerging omics technique, metabolomics reflects the metabolic state influenced by both genetic and environmental factors and has been increasingly applied to hearing loss research. This review summarizes the latest advances in metabolomics applied to several types of acquired hearing loss, including age-related hearing loss, noise-induced hearing loss, and sudden sensorineural hearing loss. Through the identification and functional enrichment of differentially expressed metabolites in samples such as inner ear tissues, perilymph, and plasma, metabolomics aims to explore the pathogenesis and identify diagnostic and prognostic biomarkers of acquired hearing loss, providing promising prospects for novel treatments. Evidence we gather from existing studies suggests potential correlations of metabolomic pathways—such as amino acid metabolism, lipid metabolism, purine and pyrimidine metabolism, and autophagy— with acquired hearing loss. Biomarkers like sphingosine also show their potential to predict its diagnosis and prognosis. Finally, we highlight the importance of standardizing the experimental design and expanding the sample size to enhance the accuracy and comparability of metabolomics results. Integration of metabolomics with multiple techniques and multidimensional information, as well as promotion of clinical transformation may be the future developmental direction in this field.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"464 ","pages":"Article 109302"},"PeriodicalIF":2.5,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cochlear neural contributions to triple network changes in tinnitus, hyperacusis & misophonia? A perspective review 耳蜗神经在耳鸣、听觉亢进和恐音症三联网变化中的作用？透视回顾

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-10 DOI: 10.1016/j.heares.2025.109305

Li Xie , Rodrigo Donoso-San Martín , Stefan Fink , Wibke Singer , Stephan M. Wolpert , Lukas Rüttiger , Marlies Knipper

{"title":"Cochlear neural contributions to triple network changes in tinnitus, hyperacusis & misophonia? A perspective review","authors":"Li Xie , Rodrigo Donoso-San Martín , Stefan Fink , Wibke Singer , Stephan M. Wolpert , Lukas Rüttiger , Marlies Knipper","doi":"10.1016/j.heares.2025.109305","DOIUrl":"10.1016/j.heares.2025.109305","url":null,"abstract":"<div><div>What do tinnitus, the perception of sounds without an internal or external source of noise, hyperacusis, the pathological hypersensitivity to noise, or misophonia, an intolerance to certain everyday noises, have in common, and what differentiates them? A large number of excellent studies focused in the last few decades on identifying the neural correlates of tinnitus, hyperacusis, or misophonia on the basis of central triple-network changes. In this perspective review we explicitly examine, possible differential and causal involvement of peripheral components as a presumptive trigger that may drive observed triple-network changes. Based on our results, we venture to hypothesize that: (i) tinnitus, hyperacusis, and misophonia can occur despite clinically normal hearing thresholds, and are likely causally independent of sex and age, (ii) tinnitus and hyperacusis, but possibly also misophonia are related to altered auditory processing that through desynchronized (tinnitus) or hyperactive (hyperacusis, misophonia) bottom-up ascending processing potentially explains the activity changes in, e.g., default or salient brain networks, as suggested in various studies of these different diseases. (iii) In misophonia a stress-induced top-down influence, as deep as the auditory nerve fibers, may be discussed as a contributor to generating misophonia-trigger sounds, a hypothesis that can be tested in future studies. We hope that the selective consideration of a possible interaction between peripheral and central components will help to minimize the greatest handicap of these pathologies to date towards successful therapy: the lack of clarification of the underlying causative mechanism of the diseases.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109305"},"PeriodicalIF":2.5,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Test-retest reliability of cortical auditory evoked potential indices of central auditory nervous system inhibition in people with and without tinnitus 伴有和不伴有耳鸣的人中枢听觉神经系统抑制的皮层听觉诱发电位指数的重测信度

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-07 DOI: 10.1016/j.heares.2025.109301

Kenneth Morse, Leah Morse

{"title":"Test-retest reliability of cortical auditory evoked potential indices of central auditory nervous system inhibition in people with and without tinnitus","authors":"Kenneth Morse, Leah Morse","doi":"10.1016/j.heares.2025.109301","DOIUrl":"10.1016/j.heares.2025.109301","url":null,"abstract":"<div><div>The purpose of this study was to determine the test-retest reliability of cortical auditory evoked potentials (CAEPs) called onset-offset and sensory gating CAEPs. Further, this study compared test-retest reliability across people with and without tinnitus, across different stimulus frequencies (broadband noise, 0.25 kHz, 2 kHz, 8 kHz), and across different CAEP quantification approaches. The results of this study indicated that both sensory gating and onset-offset CAEPs were reliable, however reliability was maximized under certain experimental conditions. Specifically, people with tinnitus did exhibit differences in reliability for the sensory gating CAEP, although this did depend on the bandpass filter that was used to analyze the sensory gating response. Further, reliability was the strongest when the stimulus was a broadband noise as opposed to frequency-specific tones. Regarding CAEP quantification approach, reliability was the strongest for CAEP types that were larger in amplitude and robust CAEP measurements including peak-to-peak amplitude and total response area. Overall, these findings do indicate that both the sensory gating and onset-offset CAEP are reliable for people with and without tinnitus. However, certain experimental methods can be applied to maximize reliability such as using a broadband noise stimulus and/or measuring peak-to-peak amplitude and total response area.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109301"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short- and long-term changes in auditory sensitivity and tinnitus distress between acute and chronic tinnitus: Longitudinal observation in a community-based sample 急性和慢性耳鸣之间听觉敏感性和耳鸣痛苦的短期和长期变化：社区样本的纵向观察

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-07 DOI: 10.1016/j.heares.2025.109299

Abishek Umashankar , Phillip Gander , Kai Alter , William Sedley

{"title":"Short- and long-term changes in auditory sensitivity and tinnitus distress between acute and chronic tinnitus: Longitudinal observation in a community-based sample","authors":"Abishek Umashankar , Phillip Gander , Kai Alter , William Sedley","doi":"10.1016/j.heares.2025.109299","DOIUrl":"10.1016/j.heares.2025.109299","url":null,"abstract":"<div><div>Chronic tinnitus is a common consequence of hearing loss, which commonly coexists with a degree of increased subjective sensitivity to sound intensity, and sometimes overt hyperacusis. Central gain is thought to be an underlying mechanism for hyperacusis, whilst its relationship with tinnitus is debated. The natural history of chronic tinnitus from its acute stages has been subject to very limited formal study, and only from within the sub-population attending specialist clinics.</div><div>We studied community-based samples of individuals with Acute Tinnitus (AT: <6 weeks from onset, N = 51), who were followed up longitudinally 6 months post-onset (N = 26), those with Chronic Tinnitus (CT: >6 months from onset, N = 51), and Non-Tinnitus controls (NT: N = 35) age/sex/hearing matched to the Acute Tinnitus group. We measured tinnitus symptoms with loudness matching, numerical rating scales (NRS), and the Tinnitus Handicap Inventory (THI) and Tinnitus Functional Index (TFI) questionnaires, and subjective auditory sensitivity with categorical loudness scaling (CLS) and the Hyperacusis Questionnaire (HQ) and Inventory of Hyperacusis Symptoms (IHS). Results revealed that measures of tinnitus (psychoacoustically matched loudness, THI, TFI) were maximal around the time of onset and reduced significantly over initial months, in line with previous reports of specialist clinic-based cohorts without high levels of initial distress, but with the additional novel demonstration of a reduction in psychoacoustically matched tinnitus loudness. Conversely, measures of auditory sensitivity (HQ, IHS, CLS slope at 1 kHz and at tinnitus frequency) did not change longitudinally or differ between AT and NT groups. We interpret these changes as indicating spontaneous habituation to the tinnitus over time, but also that subjective auditory sensitivity is not necessarily directly linked to tinnitus symptoms.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109299"},"PeriodicalIF":2.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EEG responses to onset-edge and steady-state segments of continuous speech under selective auditory attention modulation 选择性听觉注意调制下连续语音起始边缘段和稳态段的脑电反应

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-04 DOI: 10.1016/j.heares.2025.109298

Lei Wang , Fei Chen

{"title":"EEG responses to onset-edge and steady-state segments of continuous speech under selective auditory attention modulation","authors":"Lei Wang , Fei Chen","doi":"10.1016/j.heares.2025.109298","DOIUrl":"10.1016/j.heares.2025.109298","url":null,"abstract":"<div><div>Electroencephalography (EEG) signals provide valuable insights into the neural mechanisms of speech perception. However, it still remains unclear how neural responses dynamically align with continuous speech under selective attention modulation in the complex auditory environments. This study examined the evoked and induced EEG responses, their correlations with speech features, and cortical distributions for the target and ignored speech in two-talker competing scenarios. Results showed that selective attention increased the evoked EEG power for the target speech compared to the ignored speech. In contrast, the induced power indicated no significant differences. Low-frequency EEG power and phase responses reliably indexed the target speech identification amid competing streams. Cortical distribution analyses revealed that the evoked power differences between the target and ignored speech were concentrated in the central and parietal cortices. Significant induced power differences between the target and ignored speech presented only at the onset-edge segments in the left temporal cortex. Comparisons between onset-edge and steady-state segments showed the evoked power differences in the right central and temporal cortices and the induced power differences in the frontal cortex for the ignored speech. No significant differences of the cortical distribution were observed between the onset-edge and steady-state segments for the target speech. These findings underscore the distinct contributions of the evoked and induced neural activities and their cortical distributions to selective auditory attention and segment-specific speech perception.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109298"},"PeriodicalIF":2.5,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Speech performance in adults with cochlear implants using combined channel deactivation and dynamic current focusing 结合通道失活与动态电流聚焦对人工耳蜗植入成人的言语表现

IF 2.5 2区医学

Hearing Research Pub Date : 2025-05-03 DOI: 10.1016/j.heares.2025.109285

Dietmar M. Wohlbauer , Charles B. Hem , Caylin McCallick , Julie G. Arenberg

{"title":"Speech performance in adults with cochlear implants using combined channel deactivation and dynamic current focusing","authors":"Dietmar M. Wohlbauer , Charles B. Hem , Caylin McCallick , Julie G. Arenberg","doi":"10.1016/j.heares.2025.109285","DOIUrl":"10.1016/j.heares.2025.109285","url":null,"abstract":"<div><h3>Objectives and Methods:</h3><div>Cochlear implant listeners show difficulties in understanding speech in noise. Channel interactions from activating overlapping neural populations reduce the signal accuracy necessary to interpret complex signals. Optimizing programming strategies based on focused detection thresholds to reduce channel interactions has led to improved performance. In the current study, two previously suggested methods, channel deactivation and focused dynamic tripolar stimulation, were combined. Utilizing an automatic channel selection algorithm from focused detection threshold profiles, three cochlear implant programs were created with the same deactivated channels but varying proportions of channels employing focused stimulation, monopolar, dynamic focused and a mixed program. Thirteen ears in eleven adult cochlear implant listeners with Advanced Bionics HiRes90k devices were tested. Vowel identification and sentence perception in quiet and noise served as outcome measures, and the influences of listening experience, age, clinical consonant-nucleus-consonant performance, and perceptual thresholds on speech performance were assessed.</div></div><div><h3>Results:</h3><div>Across subjects, different degrees of focusing showed individual performance improvements for vowels and sentences over the monopolar program. Focused listening benefits were shown for individuals with less cochlear implant experience, and clinically poor performers seem to benefit more from focusing than good performers. However, only slight trends and no significant group improvements were observed.</div></div><div><h3>Conclusion:</h3><div>The current findings suggest that deactivating and focusing subsets of channels might improve speech performance for some individuals, especially poor performers, a possible effect of reduced channel interactions. The findings also show that performance is largely variable among individuals.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"463 ","pages":"Article 109285"},"PeriodicalIF":2.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of bone conduction stimulation efficiency on the skull surface and below assessed by cochlear promontory vibration 耳蜗峡部振动评价颅骨表面及以下骨传导刺激效率的研究

IF 2.5 2区医学

Hearing Research Pub Date : 2025-04-24 DOI: 10.1016/j.heares.2025.109280

Alexander du Puits , Kristian Åsnes , Thomas Lenarz , Hannes Maier

{"title":"Investigation of bone conduction stimulation efficiency on the skull surface and below assessed by cochlear promontory vibration","authors":"Alexander du Puits , Kristian Åsnes , Thomas Lenarz , Hannes Maier","doi":"10.1016/j.heares.2025.109280","DOIUrl":"10.1016/j.heares.2025.109280","url":null,"abstract":"<div><h3>Objective</h3><div>Stimulation sites closer to the cochlea result in higher output for bone conduction devices (BCD), increasing maximum output and reducing energy consumption. In our study, alternative coupling sites closer to the ear canal (EC), in cortical and spongious bone, were investigated and the influence on ipsi- and contralateral bone conduction output was quantified.</div></div><div><h3>Methods</h3><div>Cochlear promontory (CP) vibrations were measured by 1D laser Doppler vibrometry, using five freshly frozen (10 ears) whole human cadaver heads. A percutaneous actuator was implanted at different positions: five on the surface 25 to 55 mm posterior to the EC and two recessed positions 20 mm posterior to the EC at 5 to 8 mm depth. Stimulation was performed using a stepped sine, consisting of 78 frequencies from 0.1 to 10 kHz.</div></div><div><h3>Results</h3><div>In comparison to positions 50–55 mm to the EC, average CP vibrations showed significantly increased CP vibrations, ranging from 6.5 dB to 13.9 dB between 0.5 and 4 kHz at closer and recessed positions. In contrast, contralateral results showed smaller output amplitudes (0.5 kHz < f < 4 kHz). For positions closer to the EC, transcranial attenuation was considerably increased up to 25.2 dB at 4 kHz.</div></div><div><h3>Conclusion</h3><div>Our results show a significant increase of CP vibrations in stimulation direction, for positions closer to the cochlea. Moreover, the feasibility of bone anchors in spongious bone was demonstrated, leading to similar output when compared to positions on the skull surface.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"462 ","pages":"Article 109280"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward an ex vivo preparation for studies of the cochlear active process in mammals 用于哺乳动物耳蜗活动过程研究的离体制备

IF 2.5 2区医学

Hearing Research Pub Date : 2025-04-24 DOI: 10.1016/j.heares.2025.109288

Francesco Gianoli , Rodrigo Alonso , Brian Fabella, A.J. Hudspeth

{"title":"Toward an ex vivo preparation for studies of the cochlear active process in mammals","authors":"Francesco Gianoli , Rodrigo Alonso , Brian Fabella, A.J. Hudspeth","doi":"10.1016/j.heares.2025.109288","DOIUrl":"10.1016/j.heares.2025.109288","url":null,"abstract":"<div><div>The mammalian cochlea benefits from an active process characterized by amplification of mechanical inputs, sharp frequency selectivity, compressive nonlinearity, and spontaneous otoacoustic emission. Similar traits are observed in individual hair cells of nonmammalian tetrapods, in which they emerge from the critical dynamical regime of hair cells operating near a Hopf bifurcation. It remains unclear whether a similar critical regime also underpins the active process of the mammalian cochlea. Efforts to address this question have been limited in part by the absence of an <em>ex vivo</em> preparation that both preserves the physiological integrity of the sensory epithelium and grants direct experimental access to it. To overcome these problems, we improved a two-compartment cochlear preparation (Chan and Hudspeth, 2005a, 2005b) to more closely simulate <em>in vivo</em> conditions and used it to conduct electrophysiological recordings of microphonic signals in isolated cochlear segments of the Mongolian gerbil. Our methodological advances included refining the dissection protocol to reduce the size of the exposed cochlear segment and altering the ionic compositions of the solutions to better control the Ca<sup>2+</sup> concentration. We also maintained a constant temperature in order to stabilize the experimental conditions. Most critically, by introducing a mechanism to adjust the pressure in the endolymphatic compartment, we were able to explore how variations in transepithelial pressure influence the electrical response. These changes enabled us to reliably measure compressive nonlinearities with a one-third power law similar to that observed from cochleas <em>in vivo</em> and consistent with the behavior of a dynamical system operating near a Hopf bifurcation.</div></div>","PeriodicalId":12881,"journal":{"name":"Hearing Research","volume":"462 ","pages":"Article 109288"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0