Future microbiology最新文献_第9页

Effects of whey protein supplementation on gut microbiota of Wistar rats with valproic acid-induced autism symptoms. 补充乳清蛋白对具有丙戊酸诱导的自闭症症状的Wistar大鼠肠道微生物群的影响。

IF 3.1 4区生物学

Future microbiology Pub Date : 2024-02-01 Epub Date: 2023-11-07 DOI: 10.2217/fmb-2023-0051

Quezia Damaris Jones Severino Vasconcelos, Marisa Jadna Silva Frederico, Renata de Sousa Alves, Tereza de Jesus Pinheiro Gomes Bandeira, Maria Elisabete Amaral de Moraes, Gislei Frota Aragão

引用次数: 0

Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant Klebsiella pneumoniae. 多粘菌素 B 与香芹酚的协同作用：针对耐多粘菌素肺炎克雷伯菌的抗菌策略。

IF 3.1 4区生物学

Future microbiology Pub Date : 2024-02-01 Epub Date: 2024-02-08 DOI: 10.2217/fmb-2023-0070

Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto

引用次数: 0

Sulbactam-durlobactam: a β-lactam/β-lactamase inhibitor combination targeting Acinetobacter baumannii. 舒巴坦-杜鲁巴坦：针对鲍曼不动杆菌的β-内酰胺/β-内酰胺酶抑制剂组合。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-03-01 DOI: 10.2217/fmb-2023-0248

Sarah M McLeod, John P O'Donnell, Navaneeth Narayanan, John P Mills, Keith S Kaye

引用次数: 0

Establishment and evaluation of MIRA-qPCR assay for the rapid and sensitively detection of Mycoplasma pneumoniae. 建立并评估用于快速灵敏检测肺炎支原体的 MIRA-qPCR 检测方法。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-09-11 DOI: 10.1080/17460913.2024.2398886

Qiao Qiao, Yi-Yue Ge, Xiao-Juan Zhu, Kang-Chen Zhao, Yin Chen, Lun-Biao Cui, Tao Wu

{"title":"Establishment and evaluation of MIRA-qPCR assay for the rapid and sensitively detection of Mycoplasma pneumoniae.","authors":"Qiao Qiao, Yi-Yue Ge, Xiao-Juan Zhu, Kang-Chen Zhao, Yin Chen, Lun-Biao Cui, Tao Wu","doi":"10.1080/17460913.2024.2398886","DOIUrl":"10.1080/17460913.2024.2398886","url":null,"abstract":"Aim: Mycoplasma pneumoniae (MP) is a common cause of respiratory infections, and its incidence has increased post-COVID-19 due to \"immune debt.\" Real-time quantitative polymerase chain reaction (qPCR) is the standard for detecting MP, but it has a lengthy detection time. This study aimed to establish a highly sensitive rapid detection method for MP.Materials & methods: We developed an integrated assay combining multienzyme isothermal rapid amplification (MIRA) with qPCR, referred to as MIRA-qPCR, for the rapid detection of MP, delivering results within approximately 40 min.Results: The analytic sensitivity of the MIRA-qPCR assay was 10 copies per reaction, and it exhibited no cross-reactivity with other respiratory pathogens, ensuring high specificity. Clinical sample analysis demonstrated higher sensitivity for MIRA-qPCR compared to qPCR reported in the literature, and 100% concordance with commercial qPCR kit.Conclusion: The MIRA-qPCR method established in this study is a promising tool for the clinical detection of MP, offering significant advantages for the rapid diagnosis of MP infections.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1455-1461"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus. 肼屈嗪对耐甲氧西林和甲氧西林敏感金黄色葡萄球菌的抗菌活性。

IF 3.1 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-01-31 DOI: 10.2217/fmb-2023-0160

Francisca B Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João B de Andrade Neto, Lisandra Juvêncio da Silva, Daniel Sampaio Rodrigues, Vitória P de Farias Cabral, Amanda Dias Barbosa, Lara E Almeida Moreira, Camille R Braga Vasconcelos, Bruno Coêlho Cavalcanti, Maria E França Rios, Jacilene Silva, Emmanuel Silva Marinho, Helcio Silva Dos Santos, Jacó Rl de Mesquita, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio V Nobre Júnior, Cecília Rocha da Silva

{"title":"Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus.","authors":"Francisca B Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João B de Andrade Neto, Lisandra Juvêncio da Silva, Daniel Sampaio Rodrigues, Vitória P de Farias Cabral, Amanda Dias Barbosa, Lara E Almeida Moreira, Camille R Braga Vasconcelos, Bruno Coêlho Cavalcanti, Maria E França Rios, Jacilene Silva, Emmanuel Silva Marinho, Helcio Silva Dos Santos, Jacó Rl de Mesquita, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio V Nobre Júnior, Cecília Rocha da Silva","doi":"10.2217/fmb-2023-0160","DOIUrl":"10.2217/fmb-2023-0160","url":null,"abstract":"Background: Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"91-106"},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139641960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clioquinol influences cell membrane, attenuates virulence factors, induces apoptosis to inhibit Candida albicans growth. Clioquinol 可影响细胞膜、减弱毒力因子、诱导细胞凋亡，从而抑制白色念珠菌的生长。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-10-17 DOI: 10.1080/17460913.2024.2408136

Zimeng You, Yaling Dai, Yuping Ran

{"title":"Clioquinol influences cell membrane, attenuates virulence factors, induces apoptosis to inhibit Candida albicans growth.","authors":"Zimeng You, Yaling Dai, Yuping Ran","doi":"10.1080/17460913.2024.2408136","DOIUrl":"10.1080/17460913.2024.2408136","url":null,"abstract":"Aim: To investigate the antifungal mechanism of clioquinol and indicate that clioquinol has potential as a novel therapeutic antifungal agent.Materials & methods: Analyze differentially expressed genes of Candida albicans treated with clioquinol using RNA-sequencing. The effects on cell wall and membrane features, virulence factors, apoptosis-induced cell death were also investigated.Results: The differentially expressed genes of C. albicans after treated with clioquinol focused on cell wall and membrane synthesis, antioxidant system and energy metabolism. Clioquinol did not change cell wall components levels while it decreased squalene epoxidase activity to influence the ergosterol biosynthesis in cell membrane. It also decreased cellular surface hydrophobicity and induced β-glucan unmasking to attenuate virulence factors. Meanwhile, clioquinol influenced enzyme activities involved in antioxidant system, citrate cycle, oxidative phosphorylation and decreased the ATP levels. Clioquinol induced apoptosis in C. albicans to exert its fungicidal activity. It induced reactive oxygen species and calcium ion elevation, leading to loss of mitochondrial membrane potential, cytochrome C release, metacaspase activation, thereby triggering apoptosis.Conclusion: Clioquinol exerted anti-C. albicans activity through influencing cell membrane, attenuating virulence factors and inducing apoptosis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1545-1557"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of amikacin-humic acid combination on Acinetobacter baumannii biofilm: an in vitro and in silico study. 阿米卡星-腐植酸复方制剂对鲍曼不动杆菌生物膜的影响：体外和硅学研究。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-10-21 DOI: 10.1080/17460913.2024.2412431

Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Sarvesh Sabarathinam, Manoj Kumar Narasimhan

{"title":"Effect of amikacin-humic acid combination on Acinetobacter baumannii biofilm: an in vitro and in silico study.","authors":"Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Sarvesh Sabarathinam, Manoj Kumar Narasimhan","doi":"10.1080/17460913.2024.2412431","DOIUrl":"10.1080/17460913.2024.2412431","url":null,"abstract":"Aim: Acinetobacter baumannii (AB) is a clinically important bacterial pathogen responsible for nosocomial infections. The biofilm-forming capability of these pathogens reduces the antibiotic penetration and its efficacy, thereby complicating the treatment. The current work aims to isolate the most potent biofilm-forming Acinetobacter species from clinical isolates of the patient samples and to evaluate the efficacy of the amikacin-humic acid combination against it.Methods: The combination effect of Amikacin-Humic (AMK-HUM) acid against the highest biofilm-producing A. baumannii SLMK001 was studied via in-vitro (microscopic analysis) and in-silico (Network Pharmacology) analysis.Results: The amikacin-humic acid combination significantly inhibited both the biofilm formation and cell viability of A. baumannii SLMK001. The images observed via Scanning Electron Microscope (SEM) showed a significant decrease in the biofilm matrix. Confocal Laser Scanning Microscope (CLSM) confirmed a reduction of the Z value of its three-dimensional structure. Further, the Network Pharmacology approach supported these experimental findings by identifying the key targets of the amikacin-humic acid combination against the biofilm pathways of A. baumannii.Conclusion: The in-vitro results aligned with the in-silico findings, indicating that the AMK-HUM combination is a promising treatment that significantly activates the key proteins against A. baumannii biofilm formation and pathogenesis.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1573-1585"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the role of VapBC toxin-antitoxin systems in Mycobacterium tuberculosis stress adaptation. 解密 VapBC 毒素-抗毒素系统在结核分枝杆菌应激适应中的作用。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-10-21 DOI: 10.1080/17460913.2024.2412447

Zoozeal Thakur, Renu Chaudhary, Promod K Mehta

{"title":"Deciphering the role of VapBC toxin-antitoxin systems in Mycobacterium tuberculosis stress adaptation.","authors":"Zoozeal Thakur, Renu Chaudhary, Promod K Mehta","doi":"10.1080/17460913.2024.2412447","DOIUrl":"10.1080/17460913.2024.2412447","url":null,"abstract":"Mycobacterium tuberculosis (Mtb) harbors a high number of Toxin-Antitoxin (TA) systems, wherein half of them belong to virulence associated proteins B and C (VapBC) family that has a characteristic PilT N-terminus domain and ribonuclease activity. Functional insights into Mtb VapBC TA modules unraveled their role in adaptation to various host-mediated stressors, including oxidative/nitrosative, chemical and nutrient starvation as well as multidrug tolerance and establishment of persistence. To understand the intricacies of Mtb's pathogenesis, absolute cellular targets of 19 VapC(s) were determined. Some exhibit a shared ribonuclease activity, whereas others harbor tRNAse and 23S rRNA cleavage activity. The detailed functional characterization of VapBC4, VapBC12 and VapBC22, including in vivo deletion mutant studies revealed their role in Mtb's virulence/persistence. For example, the VapC22 mutant was attenuated for Mtb's growth in mice and elicited a decreased TH1 response, whereas mice infected with VapC12 mutant displayed a substantially higher bacillary load and pro-inflammatory response than the wild type, showing a hyper-virulent phenotype. Further experimental studies are needed to decode the functional role of VapBC systems and unravel their cellular targets. Taken together, Mtb VapBC TA systems seem to be promising drug targets owing to their key role in enduring stressors, antibiotic resistance and persistence.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1587-1599"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142463283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on the use of ceftolozane/tazobactam: a review of clinical trial data and real-world evidence. 头孢妥仑/他唑巴坦的使用前景：临床试验数据和实际证据回顾。

IF 2.5 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-01-22 DOI: 10.2217/fmb-2023-0197

Ignacio Martin-Loeches, Christopher J Bruno, C Andrew DeRyke

引用次数: 0

Triple in silico targeting of IMPDH enzyme and RNA-dependent RNA polymerase of both SARS-CoV-2 and Rhizopus oryzae. 对 SARS-CoV-2 和根瘤蚜的 IMPDH 酶和 RNA 依赖性 RNA 聚合酶进行三重硅学靶向。

IF 3.1 4区生物学

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-01-31 DOI: 10.2217/fmb-2023-0103

Abdel-Moniem S Hassan, Abdo A Elfiky, Alaa M Elgohary

引用次数: 0