Glycobiology最新文献_第7页

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag053

Aminul Islam

引用次数: 0

Sialylation profile and Siglec-E expression across tissues in the B16OVA melanoma mouse model. B16OVA黑色素瘤小鼠模型中唾液酰化谱和siglece在各组织中的表达

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag041

Magali Coccimiglio, Tao Zhang, Katarzyna Olesek, Laura Goossens-Kruijssen, Noortje de Haan, Fabrizio Chiodo, Yvette van Kooyk

{"title":"Sialylation profile and Siglec-E expression across tissues in the B16OVA melanoma mouse model.","authors":"Magali Coccimiglio, Tao Zhang, Katarzyna Olesek, Laura Goossens-Kruijssen, Noortje de Haan, Fabrizio Chiodo, Yvette van Kooyk","doi":"10.1093/glycob/cwag041","DOIUrl":"10.1093/glycob/cwag041","url":null,"abstract":"Increased sialylation of tumour cells, which favours tumour growth and immune evasion, has been described using in vitro and in vivo models, leading to the first in-human clinical trial targeting sialylation in cancer. One important limitation is that the biology of sialic acids and their receptors (Siglecs), which have been considered as immune checkpoints, is different between mice and human. Hence, it is crucial to fully describe and investigate sialic acids and Siglecs expression in animal models, to define their advantages and limitations. Here, we determined the sialylation profile of the widely-used B16OVA melanoma mouse model using flow cytometry and glycomics. B16OVA cells express Siglec-E-binding sialoglycans, therefore we explored Siglec-E expression across various tissues from tumour-bearing mice. We identified that Siglec-E is expressed on myeloid cells and CD8+ T cells in the tumour microenvironment. However, in spleen, blood and tumour-draining lymph nodes not only CD8+ but also CD4+ T cells expressed Siglec-E. Interestingly, Siglec-E+ and Siglec-E- T cells presented distinct expression of PD-1 across tissues, suggesting different regulation mechanisms for the expression of these immune checkpoints. Our work provides an investigation of the sialoglycans on B16OVA cells and the expression of their receptor Siglec-E across tissues, which is of importance for future therapeutic studies targeting the sialic acids-Siglec axis, especially in combination with anti-PD-1 therapies.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148137550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco-Forum.

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag051

Karen Colley

引用次数: 0

One-pot synthesis of lacto-N-tetraose using a glycosynthase engineered from GH136 lacto-N-biosidase LnbX. 以GH136乳酸- n -生物苷酶LnbX为原料的糖合酶一锅合成乳酸- n -四糖。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag054

Noriki Fujio, Ryota Umekage, Ami Ishikawa, Yuji Honda, Yuta Sugiyama, Motomitsu Kitaoka, Takane Katayama, Shinya Fushinobu, Chihaya Yamada

{"title":"One-pot synthesis of lacto-N-tetraose using a glycosynthase engineered from GH136 lacto-N-biosidase LnbX.","authors":"Noriki Fujio, Ryota Umekage, Ami Ishikawa, Yuji Honda, Yuta Sugiyama, Motomitsu Kitaoka, Takane Katayama, Shinya Fushinobu, Chihaya Yamada","doi":"10.1093/glycob/cwag054","DOIUrl":"10.1093/glycob/cwag054","url":null,"abstract":"Lacto-N-tetraose (LNT: Galβ1-3GlcNAcβ1-3Galβ1-4Glc) is the most abundant core structure of human milk oligosaccharides (HMOs) and plays important roles in establishing healthy microbiota in the infant gut. Several enzymatic strategies have been developed for the synthesis of LNT. In the conventional linear approach, LNT is synthesized by the sequential addition of N-acetylglucosamine (GlcNAc) and galactose (Gal) to lactose (Lac) through glycosidase-catalyzed transglycosylation or glycosyltransferase reactions. By contrast, the convergent approach developed more recently involves enzymatic coupling of preformed lacto-N-biose I (LNB, Galβ1-3GlcNAc) derivatives to Lac, with relatively high yields. Here, we propose a new convergent one-pot, two-enzyme strategy for LNT synthesis using GH112 galacto-N-biose/LNB phosphorylase (GLNBP) and a glycosynthase mutant of GH136 lacto-N-biosidase (LnbX). In the first reaction, α-LNB-fluoride (LNB-F) was produced from galactose-1-phosphate (Gal-1P) and α-GlcNAc-fluoride by GLNBP. In the second reaction, LNB-F is coupled to Lac using a glycosynthase variant LnbX D418S to yield LNT. Increasing the Lac concentration to 200 mM improved the apparent yield to 49%, based on the initial amount of Gal-1P. Structural analysis of the LnbX D418S-LNB complex revealed that the hydroxy group of Ser418 is positioned to facilitate the departure of the fluoride group during glycosyl transfer, providing mechanistic insight into the observed glycosynthase activity.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148420638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping ST3GAL transferase specificities in the glycosylation landscape of N/TERT-1 keratinocytes using Glycogenomics and mass spectrometry. 利用糖基因组学和质谱技术绘制N/TERT-1角质形成细胞糖基化格局中的ST3GAL转移酶特异性

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag044

Agnes L Hipgrave Ederveen, Ming Song, Tao Zhang, Jordy van Angeren, Ieva Bagdonaite, Sally Dabelsteen, Hans H Wandall, Noortje de Haan

{"title":"Mapping ST3GAL transferase specificities in the glycosylation landscape of N/TERT-1 keratinocytes using Glycogenomics and mass spectrometry.","authors":"Agnes L Hipgrave Ederveen, Ming Song, Tao Zhang, Jordy van Angeren, Ieva Bagdonaite, Sally Dabelsteen, Hans H Wandall, Noortje de Haan","doi":"10.1093/glycob/cwag044","DOIUrl":"10.1093/glycob/cwag044","url":null,"abstract":"Glycan sialylation is vital for proper cellular function and signaling. The six-membered ST3GAL family of sialyltransferases catalyzes the transfer of sialic acid in an α2,3-linkage to galactose residues on the outermost glycan epitopes. Dysregulation of sialyltransferase activity has been linked to diverse pathological processes. To expand our understanding of the substrate specificity and cooperative function of the ST3GAL family enzymes in protein and lipid glycosylation, we systematically analyzed the function of individual ST3GAL enzymes in glycan biosynthesis using a panel of CRISPR/Cas9-engineered human keratinocyte (N/TERT-1) cell lines with single or combined ST3GAL gene knockouts (KO). For protein glycosylation, KO of ST3GAL1 reduced sialylation of type 3 epitopes (Galβ1,3-GalNAc-) on both core 1 and 2 O-glycans, while complete ablation of sialylation was observed for the combined ST3GAL1 and ST3GAL2 KO. ST3GAL2 KO alone had limited effect, but reduced sialylation of specifically core 1 O-glycans. KO of ST3GAL4 and the combined KO of ST3GAL4 and ST3GAL6 reduced sialylation of type 1 and 2 epitopes (Galβ1,3/4-GlcNAc-) on both N- and O-glycans, while no effect was observed for the single KO of ST3GAL6. In GSL glycan biosynthesis, ST3GAL5 regulated lactosylceramide sialylation, as anticipated. ST3GAL2 and ST3GAL6 mediate sialylation of type 3 motifs, whereas ST3GAL3 and ST3GAL6 target type 2 epitopes, with ST3GAL3 exhibiting no discernible preference between type 1 and type 2 substrates. Our findings reveal that glycosyltransferase specificities are shaped by substrate availability, epitope distribution across glycan classes, and enzyme competition, which can only be captured by investigating this within the cellular context.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13284783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148217453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of human milk oligosaccharides (HMOs) in glycoengineered human cells. 人乳寡糖（HMOs）在糖工程人细胞中的生物合成。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag048

Stijn Kruf, Roy J B M Delahaije, Khadra A Mohamed, Barry Schoemaker, Yoshiki Narimatsu, Henrik Clausen, Vassilis Triantis, Thomas J Boltje, Christian Büll

{"title":"Biosynthesis of human milk oligosaccharides (HMOs) in glycoengineered human cells.","authors":"Stijn Kruf, Roy J B M Delahaije, Khadra A Mohamed, Barry Schoemaker, Yoshiki Narimatsu, Henrik Clausen, Vassilis Triantis, Thomas J Boltje, Christian Büll","doi":"10.1093/glycob/cwag048","DOIUrl":"10.1093/glycob/cwag048","url":null,"abstract":"Human milk oligosaccharides (HMOs) are unconjugated and structurally diverse glycans synthesized in the lactating mammary gland through the stepwise action of glycosyltransferases that extend a free lactose core. Several HMOs are capped with sialic acids, including 3'-sialyllactose (3'-SL) and 6'-sialyllactose (6'-SL), that promote early-life microbiota development and contribute to immune system and neuronal functions. These health-promoting properties make sialylated HMOs attractive biomolecules for incorporation in infant nutrition and functional food products. Mammalian cell lines lack endogenous HMO production, limiting mechanistic studies of HMO biosynthesis and constraining production strategies based on human cells. Here, we developed a human cell-based strategy for the production of the two common sialyllactose isomers 3'-SL and 6'-SL in glycoengineered human embryonic kidney (HEK293) cells. We co-expressed LALBA and B4GALT1, that together form the lactose synthase complex, to introduce free lactose biosynthesis capacity into a genetically engineered human cell line without sialylation (HEK293ΔSia). Stable expression of either ST3GAL or ST6GAL isoenzymes in HEK293ΔSia cells revealed that ST3GAL3/4/5, and especially ST3GAL5, efficiently convert lactose into 3'-SL while ST6GAL1 and ST6GAL2 produce the 6'-SL isomer. These results provide insights into the in vivo ability of sialyltransferase isoenzymes to use lactose as substrate. Establishing HMOs biosynthesis pathways into controllable human cell systems offers an alternative strategy for production of HMOs and provides a starting point to unlock biosynthesis of more complex HMOs in human cells.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148294845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct properties of Halobacterium salinarum Agl32, an archaeal D-glucuronyl C5-epimerase involved in N-glycosylation. Hbt的独特性质。Salinarum Agl32，一种参与n -糖基化的古菌d -葡萄糖醛基C5-epimerase。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag045

Antonella Aquilone, Yarin Levi, Marianna Zaretsky, Zlata Vershinin, Iris Grossman-Haham, Cristina De Castro, Jerry Eichler

{"title":"Distinct properties of Halobacterium salinarum Agl32, an archaeal D-glucuronyl C5-epimerase involved in N-glycosylation.","authors":"Antonella Aquilone, Yarin Levi, Marianna Zaretsky, Zlata Vershinin, Iris Grossman-Haham, Cristina De Castro, Jerry Eichler","doi":"10.1093/glycob/cwag045","DOIUrl":"10.1093/glycob/cwag045","url":null,"abstract":"The N-linked tetrasaccharide decorating glycoproteins of the halophilic archaea Halobacterium salinarum offered the first example of N-glycosylation outside the Eukarya and still represents the only known instance of iduronic acid (IdoA) being employed in this post-translational modification. Recent identification of Agl32 as the D-glucuronyl C5-epimerase catalyzing the conversion of glucuronic acid (GlcA) into IdoA allows for comparing this enzyme in each of the three domains of life, namely, Eukarya, Bacteria and Archaea. Specifically, the current study assessed whether Agl32 requires flanking sugars on either side of the target GlcA, as do its eukaryal and bacterial counterparts. Nuclear magnetic resonance analysis of the glycan from an Hbt. salinarum mutant unable to add the fourth and final N-linked tetrasaccharide sugar revealed that Agl32 requires GlcA on both sides of the target GlcA for the epimerization reaction. Despite similar requirements for flanking sugars, Agl32 shares little structural similarity with eukaryal GlcE or DSepi1, D-glucuronyl C5-epimerases respectively involved in IdoA generation in the glycosaminoglycans heparin/heparan sulfate and dermatan sulfate. Moreover, Agl32 processes a substrate far shorter than what is recognized by the eukaryal enzymes. Indeed, it would appear that Agl32 relies on a catalytic mechanism distinct from that employed by these other D-glucuronyl C5-epimerases. Finally, the presence of agl32 homologues in putative N-glycosylation gene clusters in other haloarchaea argues that the use of IdoA in N-glycosylation extends beyond Hbt. salinarum.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13308652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148217518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aberrant O-glycosylation genes and miR-21-5p targets define a molecular signature of tumor aggressiveness in triple-negative breast cancer. 异常的o糖基化基因和miR-21-5p靶点定义了三阴性乳腺癌中肿瘤侵袭性的分子特征。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-05-22 DOI: 10.1093/glycob/cwag046

Alexis Germán Murillo Carrasco, Mercedes Landeira, Tatiane Katsue Furuya, Maria José Ferreira Alves, Maitê Lobo Kolarik, Leandro Machado Colli, Patricia Pintor Dos Reis, Roger Chammas, Teresa Freire

{"title":"Aberrant O-glycosylation genes and miR-21-5p targets define a molecular signature of tumor aggressiveness in triple-negative breast cancer.","authors":"Alexis Germán Murillo Carrasco, Mercedes Landeira, Tatiane Katsue Furuya, Maria José Ferreira Alves, Maitê Lobo Kolarik, Leandro Machado Colli, Patricia Pintor Dos Reis, Roger Chammas, Teresa Freire","doi":"10.1093/glycob/cwag046","DOIUrl":"10.1093/glycob/cwag046","url":null,"abstract":"Aberrant O-glycosylation and miRNA dysregulation are established features of tumor aggressiveness, particularly in triple-negative breast cancer (TNBC). Using a Cosmc-silenced 4T1 murine model (Tn+), characterized by truncated O-glycans and enhanced metastatic potential, tissue and serum miRNA profiling identified miR-21-5p as a circulating marker associated with the Tn+ phenotype. Transcriptomic analysis revealed that miR-21-5p targets multiple tumor suppressor genes, including Btg2, Spry1, Tbx2, Rhob, and Dusp8, suggesting its involvement in epithelial-mesenchymal transition and immune modulation. Integrative single-cell RNA sequencing (scRNA-seq) of the 4T1 tumor microenvironment revealed distinct cellular clusters with inverse expression patterns of GALNT enzymes (involved in Tn synthesis) and miR-21-5p target genes, defining Tn+-like and Tn--like subpopulations. Translating these findings to human breast cancer (TCGA-BRCA), a prognostic model combining clinical variables (age, metastasis, PAM50 subtype) with three-gene expression (OLR1, PCSK6, and GALNT6) significantly improved patient risk stratification (P = 0.015). By integrating multi-omics analyses (scRNA-seq, TCGA) with an aggressive Tn+ TNBC model, this study defines a novel three-gene prognostic signature that links the glyco-miRNA axis to tumor aggressiveness, offering a promising tool for advanced patient stratification and the development of precision glyco-therapeutics.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148328886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco you should know. Glyco你应该知道。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-04-25 DOI: 10.1093/glycob/cwag033

Mateu Montserrat-Canals

引用次数: 0

Glyco you should know. Glyco你应该知道。

IF 3.3 3区生物学

Glycobiology Pub Date : 2026-04-25 DOI: 10.1093/glycob/cwag034

Natalie Stewart

引用次数: 0