Glycobiology最新文献_第7页

Variability of human Alpha-1-acid glycoprotein N-glycome in a Caucasian population. 高加索人群中人类α-1-酸糖蛋白 N-糖蛋白的变异性。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-24 DOI: 10.1093/glycob/cwae031

Frano Vučković, Mislav Novokmet, Dinko Šoić, Jerko Štambuk, Ivana Kolčić, Ozren Polašek, Gordan Lauc, Olga Gornik, Toma Keser

{"title":"Variability of human Alpha-1-acid glycoprotein N-glycome in a Caucasian population.","authors":"Frano Vučković, Mislav Novokmet, Dinko Šoić, Jerko Štambuk, Ivana Kolčić, Ozren Polašek, Gordan Lauc, Olga Gornik, Toma Keser","doi":"10.1093/glycob/cwae031","DOIUrl":"10.1093/glycob/cwae031","url":null,"abstract":"Aim: Alpha-1-acid glycoprotein (AGP) is a highly glycosylated protein in human plasma and one of the most abundant acute phase proteins in humans. Glycosylation plays a crucial role in its biological functions, and alterations in AGP N-glycome have been associated with various diseases and inflammatory conditions. However, large-scale studies of AGP N-glycosylation in the general population are lacking.Methods: Using recently developed high-throughput glycoproteomic workflow for site-specific AGP N-glycosylation analysis, 803 individuals from the Croatian island of Korcula were analyzed and their AGP N-glycome data associated with biochemical and physiological traits, as well as different environmental factors.Results: After regression analysis, we found that AGP N-glycosylation is strongly associated with sex, somewhat less with age, along with multiple biochemical and physiological traits (e.g. BMI, triglycerides, uric acid, glucose, smoking status, fibrinogen).Conclusion: For the first time we have extensively explored the inter-individual variability of AGP N-glycome in a general human population, demonstrating its changes with sex, age, biochemical, and physiological status of individuals, providing the baseline for future population and clinical studies.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":"34 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco You Should Know. 你应该知道的 Glyco

IF 4.3 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae022

Emily Kukan

引用次数: 0

Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein. 内糖苷酶的位点特异性固定揭示了 QSOX2 是一种新型多糖苷化蛋白。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae026

Carmanah Hunter, Tahlia Derksen, Sogand Makhsous, Matt Doll, Samantha Rodriguez Perez, Nichollas E Scott, Lisa M Willis

{"title":"Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein.","authors":"Carmanah Hunter, Tahlia Derksen, Sogand Makhsous, Matt Doll, Samantha Rodriguez Perez, Nichollas E Scott, Lisa M Willis","doi":"10.1093/glycob/cwae026","DOIUrl":"10.1093/glycob/cwae026","url":null,"abstract":"Polysialic acid (polySia) is a linear polymer of α2,8-linked sialic acid residues that is of fundamental biological interest due to its pivotal roles in the regulation of the nervous, immune, and reproductive systems in healthy human adults. PolySia is also dysregulated in several chronic diseases, including cancers and mental health disorders. However, the mechanisms underpinning polySia biology in health and disease remain largely unknown. The polySia-specific hydrolase, endoneuraminidase NF (EndoN), and the catalytically inactive polySia lectin EndoNDM, have been extensively used for studying polySia. However, EndoN is heat stable and remains associated with cells after washing. When studying polySia in systems with multiple polysialylated species, the residual EndoN that cannot be removed confounds data interpretation. We developed a strategy for site-specific immobilization of EndoN on streptavidin-coated magnetic beads. We showed that immobilizing EndoN allows for effective removal of the enzyme from samples, while retaining hydrolase activity. We used the same strategy to immobilize the polySia lectin EndoNDM, which enabled the enrichment of polysialylated proteins from complex mixtures such as serum for their identification via mass spectrometry. We used this methodology to identify a novel polysialylated protein, QSOX2, which is secreted from the breast cancer cell line MCF-7. This method of site-specific immobilization can be utilized for other enzymes and lectins to yield insight into glycobiology.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11031136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140136669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asian Community of Glycoscience and Glycotechnology (ACGG) conference report 2023. 亚洲糖科学和糖技术协会（ACGG）2023 年会议报告。

IF 4.3 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae017

Kavita Y Hiremath

引用次数: 0

Crystal structure of mango α1,3/α1,4-fucosyltransferase elucidates unique elements that regulate Lewis A-dominant oligosaccharide assembly. 芒果α1,3/α1,4-岩藻糖基转移酶的晶体结构阐明了调控路易斯a型寡糖组装的独特因素。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae015

Takahiro Okada, Takamasa Teramoto, Hideyuki Ihara, Yoshitaka Ikeda, Yoshimitsu Kakuta

{"title":"Crystal structure of mango α1,3/α1,4-fucosyltransferase elucidates unique elements that regulate Lewis A-dominant oligosaccharide assembly.","authors":"Takahiro Okada, Takamasa Teramoto, Hideyuki Ihara, Yoshitaka Ikeda, Yoshimitsu Kakuta","doi":"10.1093/glycob/cwae015","DOIUrl":"10.1093/glycob/cwae015","url":null,"abstract":"In various organisms, α1,3/α1,4-fucosyltransferases (CAZy GT10 family enzymes) mediate the assembly of type I (Galβ1,3GlcNAc) and/or type II (Galβ1,4GlcNAc)-based Lewis structures that are widely distributed in glycoconjugates. Unlike enzymes of other species, plant orthologues show little fucosyltransferase activity for type II-based glycans and predominantly catalyze the assembly of the Lewis A structure [Galβ1,3(Fucα1,4)GlcNAc] on the type I disaccharide unit of their substrates. However, the structural basis underlying this unique substrate selectivity remains elusive. In this study, we investigated the structure-function relationship of MiFUT13A, a mango α1,3/α1,4-fucosyltransferase. The prepared MiFUT13A displayed distinct α1,4-fucosyltransferase activity. Consistent with the enzymatic properties of this molecule, X-ray crystallography revealed that this enzyme has a typical GT-B fold-type structure containing a set of residues that are responsible for its SN2-like catalysis. Site-directed mutagenesis and molecular docking analyses proposed a rational binding mechanism for type I oligosaccharides. Within the catalytic cleft, the pocket surrounding Trp121 serves as a binding site, anchoring the non-reducing terminal β1,3-galactose that belongs to the type I disaccharide unit. Furthermore, Glu177 was postulated to function as a general base catalyst through its interaction with the 4-hydroxy group of the acceptor N-acetylglucosamine residue. Adjacent residues, specifically Thr120, Thr157 and Asp175 were speculated to assist in binding of the reducing terminal residues. Intriguingly, these structural elements were not fully conserved in mammalian orthologue which also shows predominant α1,4-fucosyltransferase activity. In conclusion, we have proposed that MiFUT13A generates the Lewis A structure on type I glycans through a distinct mechanism, divergent from that of mammalian enzymes.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139905458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosylation as a tracer of off-target Cre-lox activation in development. 糖基化作为发育过程中 Cre-lox 脱靶激活的示踪剂。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae023

Leandre M Glendenning, Kalob M Reynero, Brian A Cobb

{"title":"Glycosylation as a tracer of off-target Cre-lox activation in development.","authors":"Leandre M Glendenning, Kalob M Reynero, Brian A Cobb","doi":"10.1093/glycob/cwae023","DOIUrl":"10.1093/glycob/cwae023","url":null,"abstract":"The Cre-lox system is one of the most widely used methods for lineage-specific and inducible genome editing in vivo. However, incomplete penetrance and off-target effects due to transient promoter expression in a stem or pluripotent precursor cell can be problematic and difficult to detect, especially if the target gene is not normally present in the fully differentiated but off-target cells. Yet, the loss of the target gene through the transient expression of Cre may impact the differentiation of those cells by virtue of transient expression in a precursor population. In these situations, off-target effects in an unknown precursor cell can, at best, complicate conclusions drawn from the model, and at worst, invalidate all data generated from that knockout strain. Thus, identifying Cre-driver promoter expression along entire cell lineages is crucial to improve rigor and reproducibility. As an example, transient expression in an early precursor cell has been documented in a variety of Cre strains such as the Tie2-based Cre-driver system that is used as an \"endothelial cell-specific\" model 1. Yet, Tie2 is now known to be transiently expressed in a stem cell upstream of both hematopoietic and endothelial cell lineages. Here, we use the Tie2 Cre-driver strain to demonstrate that due to its ubiquitous nature, plasma membrane glycans are a useful marker of both penetrance and specificity of a Cre-based knockout.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11031139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemo-enzymatic synthesis of tetrasaccharide linker peptides to study the divergent step in glycosaminoglycan biosynthesis. 用化学酶法合成四糖连接肽，研究糖胺聚糖生物合成的分化步骤。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae016

Marie Bourgeais, Farah Fouladkar, Margot Weber, Elisabetta Boeri-Erba, Rebekka Wild

{"title":"Chemo-enzymatic synthesis of tetrasaccharide linker peptides to study the divergent step in glycosaminoglycan biosynthesis.","authors":"Marie Bourgeais, Farah Fouladkar, Margot Weber, Elisabetta Boeri-Erba, Rebekka Wild","doi":"10.1093/glycob/cwae016","DOIUrl":"10.1093/glycob/cwae016","url":null,"abstract":"Glycosaminoglycans are extended linear polysaccharides present on cell surfaces and within the extracellular matrix that play crucial roles in various biological processes. Two prominent glycosaminoglycans, heparan sulfate and chondroitin sulfate, are covalently linked to proteoglycan core proteins through a common tetrasaccharide linker comprising glucuronic acid, galactose, galactose, and xylose moities. This tetrasaccharide linker is meticulously assembled step by step by four Golgi-localized glycosyltransferases. The addition of the fifth sugar moiety, either N-acetylglucosamine or N-acetylgalactosamine, initiates further chain elongation, resulting in the formation of heparan sulfate or chondroitin sulfate, respectively. Despite the fundamental significance of this step in glycosaminoglycan biosynthesis, its regulatory mechanisms have remained elusive. In this study, we detail the expression and purification of the four linker-synthesizing glycosyltransferases and their utilization in the production of fluorescent peptides carrying the native tetrasaccharide linker. We generated five tetrasaccharide peptides, mimicking the core proteins of either heparan sulfate or chondroitin sulfate proteoglycans. These peptides were readily accepted as substrates by the EXTL3 enzyme, which adds an N-acetylglucosamine moiety, thereby initiating heparan sulfate biosynthesis. Importantly, EXTL3 showed a preference towards peptides mimicking the core proteins of heparan sulfate proteoglycans over the ones from chondroitin sulfate proteoglycans. This suggests that EXTL3 could play a role in the decision-making step during glycosaminoglycan biosynthesis. The innovative strategy for chemo-enzymatic synthesis of fluorescent-labeled linker-peptides promises to be instrumental in advancing future investigations into the initial steps and the divergent step of glycosaminoglycan biosynthesis.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11031135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The significant role of glycosaminoglycans in tooth development. 糖胺聚糖在牙齿发育中的重要作用。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae024

Toshihiro Inubushi, Priyanka Nag, Jun-Ichi Sasaki, Yuki Shiraishi, Takashi Yamashiro

{"title":"The significant role of glycosaminoglycans in tooth development.","authors":"Toshihiro Inubushi, Priyanka Nag, Jun-Ichi Sasaki, Yuki Shiraishi, Takashi Yamashiro","doi":"10.1093/glycob/cwae024","DOIUrl":"10.1093/glycob/cwae024","url":null,"abstract":"This review delves into the roles of glycosaminoglycans (GAGs), integral components of proteoglycans, in tooth development. Proteoglycans consist of a core protein linked to GAG chains, comprised of repeating disaccharide units. GAGs are classified into several types, such as hyaluronic acid, heparan sulfate, chondroitin sulfate, dermatan sulfate, and keratan sulfate. Functioning as critical macromolecular components within the dental basement membrane, these GAGs facilitate cell adhesion and aggregation, and play key roles in regulating cell proliferation and differentiation, thereby significantly influencing tooth morphogenesis. Notably, our recent research has identified the hyaluronan-degrading enzyme Transmembrane protein 2 (Tmem2) and we have conducted functional analyses using mouse models. These studies have unveiled the essential role of Tmem2-mediated hyaluronan degradation and its involvement in hyaluronan-mediated cell adhesion during tooth formation. This review provides a comprehensive summary of the current understanding of GAG functions in tooth development, integrating insights from recent research, and discusses future directions in this field.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11031142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Galectin-3 does not interact with RNA directly. 更正为Galectin-3 并不直接与 RNA 相互作用。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae027

引用次数: 0

Correction to: Site-specific glycosylation analysis of epidermal growth factor receptor 2 (ErbB2): exploring structure and function toward therapeutic targeting. Correction to：表皮生长因子受体 2（ErbB2）的位点特异性糖基化分析：探索治疗靶点的结构和功能。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-19 DOI: 10.1093/glycob/cwae018

引用次数: 0