Glycobiology最新文献_第10页

A hierarchical structure in the N-glycosylation process governs the N-glycosylation output: prolonged cultivation induces glycoenzymes expression variations that are reflected in the cellular N-glycome but not in the protein and site-specific glycoprofile of CHO cells. N-糖基化过程中的分层结构制约着 N-糖基化的产出：长期培养诱导糖酶表达的变化反映在细胞的 N-糖收入中，但不反映在 CHO 细胞的蛋白质和特定位点的糖图谱中。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-06-22 DOI: 10.1093/glycob/cwae045

Ilaria Arigoni-Affolter, Marie-Estelle Losfeld, René Hennig, Erdmann Rapp, Markus Aebi

{"title":"A hierarchical structure in the N-glycosylation process governs the N-glycosylation output: prolonged cultivation induces glycoenzymes expression variations that are reflected in the cellular N-glycome but not in the protein and site-specific glycoprofile of CHO cells.","authors":"Ilaria Arigoni-Affolter, Marie-Estelle Losfeld, René Hennig, Erdmann Rapp, Markus Aebi","doi":"10.1093/glycob/cwae045","DOIUrl":"10.1093/glycob/cwae045","url":null,"abstract":"N-glycosylation is a central component in the modification of secretory proteins. One characteristic of this process is a heterogeneous output. The heterogeneity is the result of both structural constraints of the glycoprotein as well as the composition of the cellular glycosylation machinery. Empirical data addressing correlations between glycosylation output and glycosylation machinery composition are seldom due to the low abundance of glycoenzymes. We assessed how differences in the glycoenzyme expression affected the N-glycosylation output at a cellular as well as at a protein-specific level. Our results showed that cellular N-glycome changes could be correlated with the variation of glycoenzyme expression, whereas at the protein level differential responses to glycoenzymes alterations were observed. We therefore identified a hierarchical structure in the N-glycosylation process: the enzyme levels in this complex pathway determine its capacity (reflected in the N-glycome), while protein-specific parameters determine the glycosite-specificity. What emerges is a highly variable and adaptable protein modification system that represents a hallmark of eukaryotic cells.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco-Forum. Glyco-Forum.

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-06-22 DOI: 10.1093/glycob/cwae044

引用次数: 0

HARRY SCHACHTER obituary. 哈里-夏赫特（HARRY SCHACHTER）讣告。

IF 4.3 3区生物学

Glycobiology Pub Date : 2024-06-19 DOI: 10.1093/glycob/cwae040

Inka Brockhausen, Jim Dennis, Paul Gleeson, Kelley Moremen, Pamela Stanley

引用次数: 0

Limited impact of cancer-derived gangliosides on anti-tumor immunity in colorectal cancer. 癌症衍生神经节苷脂对大肠癌抗肿瘤免疫的影响有限。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae036

Irene van der Haar Àvila, Tao Zhang, Victor Lorrain, Florance de Bruin, Tianne Spreij, Hitoshi Nakayama, Kazuhisa Iwabuchi, Juan J García-Vallejo, Manfred Wuhrer, Yvette van Kooyk, Sandra J van Vliet

{"title":"Limited impact of cancer-derived gangliosides on anti-tumor immunity in colorectal cancer.","authors":"Irene van der Haar Àvila, Tao Zhang, Victor Lorrain, Florance de Bruin, Tianne Spreij, Hitoshi Nakayama, Kazuhisa Iwabuchi, Juan J García-Vallejo, Manfred Wuhrer, Yvette van Kooyk, Sandra J van Vliet","doi":"10.1093/glycob/cwae036","DOIUrl":"10.1093/glycob/cwae036","url":null,"abstract":"Aberrant glycosylation is a key mechanism employed by cancer cells to evade immune surveillance, induce angiogenesis and metastasis, among other hallmarks of cancer. Sialic acids, distinctive terminal glycan structures located on glycoproteins or glycolipids, are prominently upregulated across various tumor types, including colorectal cancer (CRC). Sialylated glycans modulate anti-tumor immune responses through their interactions with Siglecs, a family of glycan-binding receptors with specificity for sialic acid-containing glycoconjugates, often resulting in immunosuppression. In this paper, we investigated the immunomodulatory function of ST3Gal5, a sialyltransferase that catalyzes the addition of α2-3 sialic acids to glycosphingolipids, since lower expression of ST3Gal5 is associated with better survival of CRC patients. We employed CRISPR/Cas9 to knock out the ST3Gal5 gene in two murine CRC cell lines MC38 and CT26. Glycomics analysis confirmed the removal of sialic acids on glycolipids, with no discernible impact on glycoprotein sialylation. Although knocking out ST3Gal5 in both cell lines did not affect in vivo tumor growth, we observed enhanced levels of regulatory T cells in CT26 tumors lacking ST3Gal5. Moreover, we demonstrate that the absence of ST3Gal5 affected size and blood vessel density only in MC38 tumors. In summary, we ascertain that sialylation of glycosphingolipids has a limited influence on the anti-tumor immune response in CRC, despite detecting alterations in the tumor microenvironment, possibly due to a shift in ganglioside abundance.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11137322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tandem-repeat lectins: structural and functional insights. 串联重复凝集素：结构和功能见解。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae041

Francisco H Olvera-Lucio, Héctor Riveros-Rosas, Adrián Quintero-Martínez, Alejandra Hernández-Santoyo

{"title":"Tandem-repeat lectins: structural and functional insights.","authors":"Francisco H Olvera-Lucio, Héctor Riveros-Rosas, Adrián Quintero-Martínez, Alejandra Hernández-Santoyo","doi":"10.1093/glycob/cwae041","DOIUrl":"10.1093/glycob/cwae041","url":null,"abstract":"Multivalency in lectins plays a pivotal role in influencing glycan cross-linking, thereby affecting lectin functionality. This multivalency can be achieved through oligomerization, the presence of tandemly repeated carbohydrate recognition domains, or a combination of both. Unlike lectins that rely on multiple factors for the oligomerization of identical monomers, tandem-repeat lectins inherently possess multivalency, independent of this complex process. The repeat domains, although not identical, display slightly distinct specificities within a predetermined geometry, enhancing specificity, affinity, avidity and even oligomerization. Despite the recognition of this structural characteristic in recently discovered lectins by numerous studies, a unified criterion to define tandem-repeat lectins is still necessary. We suggest defining them multivalent lectins with intrachain tandem repeats corresponding to carbohydrate recognition domains, independent of oligomerization. This systematic review examines the folding and phyletic diversity of tandem-repeat lectins and refers to relevant literature. Our study categorizes all lectins with tandemly repeated carbohydrate recognition domains into nine distinct folding classes associated with specific biological functions. Our findings provide a comprehensive description and analysis of tandem-repeat lectins in terms of their functions and structural features. Our exploration of phyletic and functional diversity has revealed previously undocumented tandem-repeat lectins. We propose research directions aimed at enhancing our understanding of the origins of tandem-repeat lectin and fostering the development of medical and biotechnological applications, notably in the design of artificial sugars and neolectins.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling interactions between Heparan sulfate and proteins based on the Heparan sulfate microarray analysis. 根据硫酸头孢菌素芯片分析，建立硫酸头孢菌素与蛋白质之间的相互作用模型。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae039

Cleber C Melo-Filho, Guowei Su, Kevin Liu, Eugene N Muratov, Alexander Tropsha, Jian Liu

{"title":"Modeling interactions between Heparan sulfate and proteins based on the Heparan sulfate microarray analysis.","authors":"Cleber C Melo-Filho, Guowei Su, Kevin Liu, Eugene N Muratov, Alexander Tropsha, Jian Liu","doi":"10.1093/glycob/cwae039","DOIUrl":"10.1093/glycob/cwae039","url":null,"abstract":"Heparan sulfate (HS), a sulfated polysaccharide abundant in the extracellular matrix, plays pivotal roles in various physiological and pathological processes by interacting with proteins. Investigating the binding selectivity of HS oligosaccharides to target proteins is essential, but the exhaustive inclusion of all possible oligosaccharides in microarray experiments is impractical. To address this challenge, we present a hybrid pipeline that integrates microarray and in silico techniques to design oligosaccharides with desired protein affinity. Using fibroblast growth factor 2 (FGF2) as a model protein, we assembled an in-house dataset of HS oligosaccharides on microarrays and developed two structural representations: a standard representation with all atoms explicit and a simplified representation with disaccharide units as \"quasi-atoms.\" Predictive Quantitative Structure-Activity Relationship (QSAR) models for FGF2 affinity were developed using the Random Forest (RF) algorithm. The resulting models, considering the applicability domain, demonstrated high predictivity, with a correct classification rate of 0.81-0.80 and improved positive predictive values (PPV) up to 0.95. Virtual screening of 40 new oligosaccharides using the simplified model identified 15 computational hits, 11 of which were experimentally validated for high FGF2 affinity. This hybrid approach marks a significant step toward the targeted design of oligosaccharides with desired protein interactions, providing a foundation for broader applications in glycobiology.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The three-sided right-handed β-helix is a versatile fold for glycan interactions. 三面右旋 "绊脚石 "螺旋是一种多用途的糖相互作用折叠结构。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae037

Audrey A Burnim, Keith Dufault-Thompson, Xiaofang Jiang

{"title":"The three-sided right-handed β-helix is a versatile fold for glycan interactions.","authors":"Audrey A Burnim, Keith Dufault-Thompson, Xiaofang Jiang","doi":"10.1093/glycob/cwae037","DOIUrl":"10.1093/glycob/cwae037","url":null,"abstract":"Interactions between proteins and glycans are critical to various biological processes. With databases of carbohydrate-interacting proteins and increasing amounts of structural data, the three-sided right-handed β-helix (RHBH) has emerged as a significant structural fold for glycan interactions. In this review, we provide an overview of the sequence, mechanistic, and structural features that enable the RHBH to interact with glycans. The RHBH is a prevalent fold that exists in eukaryotes, prokaryotes, and viruses associated with adhesin and carbohydrate-active enzyme (CAZyme) functions. An evolutionary trajectory analysis on structurally characterized RHBH-containing proteins shows that they likely evolved from carbohydrate-binding proteins with their carbohydrate-degrading activities evolving later. By examining three polysaccharide lyase and three glycoside hydrolase structures, we provide a detailed view of the modes of glycan binding in RHBH proteins. The 3-dimensional shape of the RHBH creates an electrostatically and spatially favorable glycan binding surface that allows for extensive hydrogen bonding interactions, leading to favorable and stable glycan binding. The RHBH is observed to be an adaptable domain capable of being modified with loop insertions and charge inversions to accommodate heterogeneous and flexible glycans and diverse reaction mechanisms. Understanding this prevalent protein fold can advance our knowledge of glycan binding in biological systems and help guide the efficient design and utilization of RHBH-containing proteins in glycobiology research.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11129586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco-Forum. Glyco-Forum.

IF 4.3 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae038

引用次数: 0

Novel genetically glycoengineered human dendritic cell model reveals regulatory roles of α2,6-linked sialic acids in DC activation of CD4+ T cells and response to TNFα. 新型基因糖工程人树突状细胞模型揭示了α2,6-连接的硅酸在直流激活 CD4 + T 细胞和对 TNFα 的反应中的调控作用。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-05-26 DOI: 10.1093/glycob/cwae042

Weihua Tian, Anne Louise Blomberg, Kaylin Elisabeth Steinberg, Betina Lyngfeldt Henriksen, Josefine Søborg Jørgensen, Kerstin Skovgaard, Sarah Line Skovbakke, Steffen Goletz

{"title":"Novel genetically glycoengineered human dendritic cell model reveals regulatory roles of α2,6-linked sialic acids in DC activation of CD4+ T cells and response to TNFα.","authors":"Weihua Tian, Anne Louise Blomberg, Kaylin Elisabeth Steinberg, Betina Lyngfeldt Henriksen, Josefine Søborg Jørgensen, Kerstin Skovgaard, Sarah Line Skovbakke, Steffen Goletz","doi":"10.1093/glycob/cwae042","DOIUrl":"10.1093/glycob/cwae042","url":null,"abstract":"Dendritic cells (DCs) are central for the initiation and regulation of appropriate immune responses. While several studies suggest important regulatory roles of sialoglycans in DC biology, our understanding is still inadequate primarily due to a lack of appropriate models. Previous approaches based on enzymatic- or metabolic-glycoengineering and primary cell isolation from genetically modified mice have limitations related to specificity, stability, and species differences. This study addresses these challenges by introducing a workflow to genetically glycoengineer the human DC precursor cell line MUTZ-3, described to differentiate and maturate into fully functional dendritic cells, using CRISPR-Cas9, thereby providing and validating the first isogenic cell model for investigating glycan alteration on human DC differentiation, maturation, and activity. By knocking out (KO) the ST6GAL1 gene, we generated isogenic cells devoid of ST6GAL1-mediated α(2,6)-linked sialylation, allowing for a comprehensive investigation into its impact on DC function. Glycan profiling using lectin binding assay and functional studies revealed that ST6GAL1 KO increased the expression of important antigen presenting and co-stimulatory surface receptors and a specifically increased activation of allogenic human CD4 + T cells. Additionally, ST6GAL1 KO induces significant changes in surface marker expression and cytokine response to TNFα-induced maturation, and it affects migration and the endocytic capacity. These results indicate that genetic glycoengineering of the isogenic MUTZ-3 cellular model offers a valuable tool to study how specific glycan structures influence human DC biology, contributing to our understanding of glycoimmunology.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucosylceramides impact cellulose deposition and cellulose synthase complex motility in Arabidopsis. 葡萄糖甘油三酯影响拟南芥中纤维素的沉积和纤维素合成酶复合体的运动。

IF 3.4 3区生物学

Glycobiology Pub Date : 2024-04-24 DOI: 10.1093/glycob/cwae035

Jose A Villalobos, Rebecca E Cahoon, Edgar B Cahoon, Ian S Wallace

{"title":"Glucosylceramides impact cellulose deposition and cellulose synthase complex motility in Arabidopsis.","authors":"Jose A Villalobos, Rebecca E Cahoon, Edgar B Cahoon, Ian S Wallace","doi":"10.1093/glycob/cwae035","DOIUrl":"10.1093/glycob/cwae035","url":null,"abstract":"Cellulose is an abundant component of plant cell wall matrices, and this para-crystalline polysaccharide is synthesized at the plasma membrane by motile Cellulose Synthase Complexes (CSCs). However, the factors that control CSC activity and motility are not fully resolved. In a targeted chemical screen, we identified the alkylated nojirimycin analog N-Dodecyl Deoxynojirimycin (ND-DNJ) as a small molecule that severely impacts Arabidopsis seedling growth. Previous work suggests that ND-DNJ-related compounds inhibit the biosynthesis of glucosylceramides (GlcCers), a class of glycosphingolipid associated with plant membranes. Our work uncovered major changes in the sphingolipidome of plants treated with ND-DNJ, including reductions in GlcCer abundance and altered acyl chain length distributions. Crystalline cellulose content was also reduced in ND-DNJ-treated plants as well as plants treated with the known GlcCer biosynthesis inhibitor N-[2-hydroxy-1-(4-morpholinylmethyl)-2-phenyl ethyl]-decanamide (PDMP) or plants containing a genetic disruption in GLUCOSYLCERAMIDE SYNTHASE (GCS), the enzyme responsible for sphingolipid glucosylation that results in GlcCer synthesis. Live-cell imaging revealed that CSC speed distributions were reduced upon treatment with ND-DNJ or PDMP, further suggesting an important relationship between glycosylated sphingolipid composition and CSC motility across the plasma membrane. These results indicate that multiple interventions compromising GlcCer biosynthesis disrupt cellulose deposition and CSC motility, suggesting that GlcCers regulate cellulose biosynthesis in plants.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0