Glycobiology最新文献

Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine. Abdala COVID-19疫苗的结构和功能糖基化。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-24 DOI: 10.1093/glycob/cwaf001

Sean A Burnap, Valeria Calvaresi, Gleysin Cabrera, Satomy Pousa, Miladys Limonta, Yassel Ramos, Luis Javier González, David J Harvey, Weston B Struwe

{"title":"Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine.","authors":"Sean A Burnap, Valeria Calvaresi, Gleysin Cabrera, Satomy Pousa, Miladys Limonta, Yassel Ramos, Luis Javier González, David J Harvey, Weston B Struwe","doi":"10.1093/glycob/cwaf001","DOIUrl":"10.1093/glycob/cwaf001","url":null,"abstract":"Abdala is a COVID-19 vaccine produced in Pichia pastoris and is based on the receptor-binding domain (RBD) of the SARS-CoV-2 spike. Abdala is currently approved for use in multiple countries with clinical trials confirming its safety and efficacy in preventing severe illness and death. Although P. pastoris is used as an expression system for protein-based vaccines, yeast glycosylation remains largely uncharacterised across immunogens. Here, we characterise N-glycan structures and their site of attachment on Abdala and show how yeast-specific glycosylation decreases binding to the ACE2 receptor and a receptor-binding motif (RBM) targeting antibody compared to the equivalent mammalian-derived RBD. Reduced receptor and antibody binding is attributed to changes in conformational dynamics resulting from N-glycosylation. These data highlight the critical importance of glycosylation in vaccine design and demonstrate how individual glycans can influence host interactions and immune recognition via protein structural dynamics.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fucosylated chondroitin sulfate, an intriguing polysaccharide from sea cucumber: past, present, and future. 聚焦硫酸软骨素，来自海参的一种有趣的多糖：过去，现在和未来。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-16 DOI: 10.1093/glycob/cwae098

Adriani L Felix, Suzane M Penno, Francisco F Bezerra, Paulo A S Mourão

{"title":"Fucosylated chondroitin sulfate, an intriguing polysaccharide from sea cucumber: past, present, and future.","authors":"Adriani L Felix, Suzane M Penno, Francisco F Bezerra, Paulo A S Mourão","doi":"10.1093/glycob/cwae098","DOIUrl":"10.1093/glycob/cwae098","url":null,"abstract":"Fucosylated chondroitin sulfate (FCS) is a unique polysaccharide, first described nearly four decades ago, and found exclusively in sea cucumbers. It is a component of the extracellular matrix, possibly associated with peculiar properties of the invertebrate tissue. The carbohydrate features a chondroitin sulfate core with branches of sulfated α-Fuc linked to position 3 of the β-GlcA. FCSs from different species of sea cucumbers share a similar chondroitin sulfate core but the structure of the sulfated α-Fuc branches varies significantly. The predominant pattern consists of a single unit of sulfated α-Fuc, though some species exhibit branches with multiple α-Fuc units. This comprehensive review focuses on four major aspects of FCS. Firstly, we describe the initial approaches to elucidate the structure of FCS using classical methods of carbohydrate chemistry. Secondly, we highlight the impact of two-dimensional NMR methods in consolidating and revealing further details about the structure of FCS. These studies were conducted by various researchers across different countries and involving multiple species of sea cucumbers. Thirdly, we summarize the biological activities reported for FCS. Our survey identified 104 publications involving FCS from 42 species of sea cucumbers, reporting 10 types of biological activities. Most studies focused on anticoagulant and antithrombotic activities. Finally, we discuss future perspectives for studies related to FCS. These studies aim to clarify the evolutionary advantage for sea cucumbers in developing such a peculiar fucosylated glycosaminoglycan. Additionally, there is a need to identify the enzymes and genes involved in the metabolism of this unique carbohydrate.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco-Forum.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-16 DOI: 10.1093/glycob/cwaf002

引用次数: 0

Obituary for Tamao Endo (1954-2024). 远藤多茂讣告（1954-2024）。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-16 DOI: 10.1093/glycob/cwae100

Hiroshi Manya

引用次数: 0

Intact N-glycopeptide analysis of human platelets reveals a Glycostructure important for platelet function.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-16 DOI: 10.1093/glycob/cwae088

Hui-Jun Zhu, Hang-Yan Dong, Cheng-Rui Qian, Qin-Qin Ma, Rui-Shu Li, Min Fu, Ye He, Ping Lu

{"title":"Intact N-glycopeptide analysis of human platelets reveals a Glycostructure important for platelet function.","authors":"Hui-Jun Zhu, Hang-Yan Dong, Cheng-Rui Qian, Qin-Qin Ma, Rui-Shu Li, Min Fu, Ye He, Ping Lu","doi":"10.1093/glycob/cwae088","DOIUrl":"https://doi.org/10.1093/glycob/cwae088","url":null,"abstract":"Glycosylation is an important posttranslational modification in platelets, and the glycosylation pattern is critical for platelet function. To date, the exploration of the roles of various glycoforms in specific platelet functions is largely lacking. In this study, a global analysis of intact N-glycopeptides in human platelets was performed to map all the glycopeptides, glycosites and glycans of platelets. The glycopeptides were enriched by the ZIC- hydrophilic interaction chromatography method and then analyzed by Liquid Chromatography-Tandem Mass Spectrometry analysis. A total of 1,425 intact glycopeptides belonging to 190 N-glycoproteins from human platelets were identified. Moreover, 358 glycans modified 328 glycosites from those glycoproteins. Functional analysis revealed that these glycoproteins are involved mainly in processes and pathways related to platelet adhesion. Among the proteins in these adhesion-related annotations, von Willebrand factor, thrombospondin 1and glycoprotein V were found to contain a possible Lewis y structure, and this finding was further verified by immunoprecipitation assays. As a blood group-related antigen, Lewis y was previously reported to exist in human platelets, but its function remains unclear. Since the glycosylation of von Willebrand factor, thrombospondin 1 and glycoprotein V is involved in platelet-collagen adhesion, the importance of Lewis y on platelet function was evaluated by adhesion assays, which demonstrated that the blockade of Lewis y on platelets decreased the adhesion of platelets to collagen I under both static and flow conditions.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":"35 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose. 用凤仙花提取物快速酶法合成GDP-L-[3H]聚焦物。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-16 DOI: 10.1093/glycob/cwae097

Jose Carlos Paredes Franco, Maria Lucia Sampaio Güther, Michael A J Ferguson

引用次数: 0

Glyco-Forum.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-13 DOI: 10.1093/glycob/cwae099

引用次数: 0

The diversity of glycan chains in jellyfish mucin of three Cubozoan species: the contrast in molecular evolution rates of the peptide chain and Glycans. 三种腔肠动物的水母粘蛋白中糖链的多样性：肽链和糖的分子进化速度对比。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-13 DOI: 10.1093/glycob/cwae090

Takuma Kaneko, Shinra Tanaka, Minami Sugiyama, Shiori Kaise, Hiroshi Inui, Kiminori Ushida

{"title":"The diversity of glycan chains in jellyfish mucin of three Cubozoan species: the contrast in molecular evolution rates of the peptide chain and Glycans.","authors":"Takuma Kaneko, Shinra Tanaka, Minami Sugiyama, Shiori Kaise, Hiroshi Inui, Kiminori Ushida","doi":"10.1093/glycob/cwae090","DOIUrl":"10.1093/glycob/cwae090","url":null,"abstract":"The O-glycan composition of jellyfish (JF) mucin (qniumucin: Q-mucin) extracted from three Cubozoan species was studied after the optimization of the purification protocol. Application of a stepwise gradient of ionic strength to anion exchange chromatography (AEXC) was effective for isolating Q-mucin from coexisting impurities. In the three species, the amino acid sequence of the tandem repeat (TR) region in Q-mucin in all three Cubozoans seemed to remain the same as that in all Scyphozoans, although their glycan chains seemed to exhibit clear diversity. In particular, the amounts of acidic moieties on the glycan chains of Q-mucin from the Cubozoans markedly varied even in these genetically close species. In two of the three Cubozoan species, the fraction of disaccharides was large, showing a sharp contrast to that of the glycans of Q-mucin in Scyphozoans. This study also indicates that the simple sequence of TR commonly inherited in all Cubozoan and Scyphozoan JF species after the long term of evolution over 500 M years. According to this research, the glycans and the TR of mucin-type glycoproteins (MTGPs), forming a hierarchical structure, appear to complement each other in the evolutionary changes because the time required for their hereditary conversion is considerably different. The cooperation of these mechanisms is a strategy to achieve the contradictory functions of biosystems, namely species conservation and diversity acquisition.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-immolative Kdn-glycoside substrate enables high-throughput screening for inhibitors of Kdnases. 一种自褪色的 Kdn-糖苷底物可实现 Kdn 酶抑制剂的高通量筛选。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-13 DOI: 10.1093/glycob/cwae094

Ali Nejatie, Cameron Proceviat, Christina Gros, Elizabeth Steves, Margo M Moore, David J Vocadlo, Andrew J Bennet

{"title":"A self-immolative Kdn-glycoside substrate enables high-throughput screening for inhibitors of Kdnases.","authors":"Ali Nejatie, Cameron Proceviat, Christina Gros, Elizabeth Steves, Margo M Moore, David J Vocadlo, Andrew J Bennet","doi":"10.1093/glycob/cwae094","DOIUrl":"10.1093/glycob/cwae094","url":null,"abstract":"Aspergillus fumigatus, a filamentous fungus, is an opportunistic pathogen and the major causative agent of the often-fatal disease, invasive aspergillosis (IA). Current treatments for IA are limited due to their high toxicity and/or the emergence of drug resistance; therefore, a need exists for the development of new therapeutics to treat IA. The Kdnase produced by A. fumigatus plays a vital role in maintaining cell wall integrity. As there are no known Kdnases in humans, developing inhibitors of Kdnase from this fungal pathogen is a promising therapeutic approach. The rapid testing of enzymatic activity in a high-throughput screen of large chemical libraries can be an efficient way to find new small molecule lead compounds. Herein we show that a Kdn glycoside with a self-immolative cleavable aglycon is a practical and efficient substrate for a high throughput assay to identify Kdnase inhibitors. We optimized the activity assay and screened over 27,000 compounds from two bioactive chemical libraries as potential inhibitors, and we compared the hit compounds' potency towards Aspergillus terreus and Trichophyton rubrum Kdnases, two other fungal Kdnases. We validated a number of hits and these small molecules are potential leads for the development of novel therapeutics to treat invasive aspergillosis.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galectin-3 disrupts tight junctions of airway epithelial cell monolayers by inducing expression and release of matrix metalloproteinases upon influenza A infection. 在感染甲型流感时，Galectin-3 通过诱导基质金属蛋白酶的表达和释放，破坏气道上皮细胞单层的紧密连接。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-01-13 DOI: 10.1093/glycob/cwae093

Muddassar Iqbal, Chiguang Feng, Guanghui Zong, Lai-Xi Wang, Gerardo R Vasta

{"title":"Galectin-3 disrupts tight junctions of airway epithelial cell monolayers by inducing expression and release of matrix metalloproteinases upon influenza A infection.","authors":"Muddassar Iqbal, Chiguang Feng, Guanghui Zong, Lai-Xi Wang, Gerardo R Vasta","doi":"10.1093/glycob/cwae093","DOIUrl":"10.1093/glycob/cwae093","url":null,"abstract":"Galectins are β-galactosyl-binding lectins with key roles in early development, immune regulation, and infectious disease. Influenza A virus (IAV) infects the airway epithelia, and in severe cases may lead to bacterial superinfections and hypercytokinemia, and eventually, to acute respiratory distress syndrome (ARDS) through the breakdown of airway barriers. The detailed mechanisms involved, however, remain poorly understood. Our prior in vivo studies in a murine model system revealed that upon experimental IAV and pneumococcal primary and secondary challenges, respectively, galectin-1 and galectin-3 (Gal-3) are released into the airway and bind to the epithelium that has been desialylated by the viral neuraminidase, contributing to secondary bacterial infection and hypercytokinemia leading to the clinical decline and death of the animals. Here we report the results of in vitro studies that reveal the role of the extracellular Gal-3 in additional detrimental effects on the host by disrupting the integrity of the airway epithelial barrier. IAV infection of the human airway epithelia cell line A549 increased release of Gal-3 and its binding to the A549 desialylated cell surface, notably to the transmembrane signaling receptors CD147 and integrin-β1. Addition of recombinant Gal-3 to A549 monolayers resulted in enhanced expression and release of matrix metalloproteinases, leading to disruption of cell-cell tight junctions, and a significant increase in paracellular permeability. This study reveals a critical mechanism involving Gal-3 that may significantly contribute to the severity of IAV infections by promoting disruption of tight junctions and enhanced permeability of the airway epithelia, potentially leading to lung edema and ARDS.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0