Glycobiology最新文献

Glycoproteomics of IgA1: uncovering key N-glycan composition in ankylosing spondylitis.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-28 DOI: 10.1093/glycob/cwaf018

Hui-Ling Chiang, Kuo-Lung Ku, Chien-Hsueh Tung, Kuang-Yung Huang, Ming-Chi Lu, Ning-Sheng Lai

{"title":"Glycoproteomics of IgA1: uncovering key N-glycan composition in ankylosing spondylitis.","authors":"Hui-Ling Chiang, Kuo-Lung Ku, Chien-Hsueh Tung, Kuang-Yung Huang, Ming-Chi Lu, Ning-Sheng Lai","doi":"10.1093/glycob/cwaf018","DOIUrl":"https://doi.org/10.1093/glycob/cwaf018","url":null,"abstract":"Objective: This study aimed to identify distinct IgA1 N-glycan composition in patients with ankylosing spondylitis (AS) compared with healthy controls and to explore their associations with inflammatory markers and disease activity indices.Methods: Serum samples were collected from 36 patients with AS and 35 healthy controls. The diagnosis of AS was based on the New York criteria. Clinical assessments included inflammatory markers (ESR, CRP, and IgA) and disease activity indices (BASDAI, ASDAS-ESR, and ASDAS-CRP). IgA1 was isolated using affinity purification and gel filtration chromatography, followed by mass spectrometry to identify N-glycans.Results: Among the 23 detected N-glycan patterns, significant differences were observed in 13 of the 18 N-glycans at the N144 site and in all five N-glycans at the N340 site between patients with AS and controls. Notably, the glycans HexNAc3Hex4NeuAc1, HexNAc4Hex4NeuAc1 and HexNAc5Hex5NeuAc1 at N144 demonstrated strong associations with all three inflammatory markers, including ESR, CRP, and IgA (P < 0.001). Levels of HexNAc4Hex4NeuAc1 were significantly elevated in patients with AS compared with those in the healthy controls. Increased sialylation and galactosylation, along with decreased fucosylation, were noted at N144 of IgA1 in patients with AS. Conversely, no glycans at N340 showed a correlation with all inflammatory markers simultaneously or with any disease activity indicators.Conclusion: IgA1 from patients with AS exhibited distinct glycosylation traits compared with controls, with elevated levels of HexNAc₄Hex₄NeuAc₁ at N144 associated with inflammatory markers. These findings suggested that differential glycosylation patterns of IgA1 may play a role in the pathogenesis of AS.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a calreticulin-targeting glycan ligand based on a hybrid binding concept.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-25 DOI: 10.1093/glycob/cwaf015

Taiki Kuribara, Taiga Kojima, Yuka Kobayashi, Mitsuaki Hirose, Keita Shibayama, Yoichi Takeda, Kiichiro Totani

{"title":"Development of a calreticulin-targeting glycan ligand based on a hybrid binding concept.","authors":"Taiki Kuribara, Taiga Kojima, Yuka Kobayashi, Mitsuaki Hirose, Keita Shibayama, Yoichi Takeda, Kiichiro Totani","doi":"10.1093/glycob/cwaf015","DOIUrl":"10.1093/glycob/cwaf015","url":null,"abstract":"Calreticulin (CRT), a chaperone that possesses both lectin and chaperone domains, is localized in the endoplasmic reticulum (ER). CRT has diverse functions and localizations; thus, CRT is a multifunctional protein. Particularly in the ER, CRT mainly aids in the proper folding of nascent glycoproteins as lectin chaperones. Approximately one-third of cellular proteins, including disease-related proteins, are synthesized in the ER. The lectin chaperones CRT and calnexin facilitate the correct folding of these glycoproteins; hence, these chaperones are essential for cells. Various CRT ligands have been reported, mainly composed of Glc1Man9GlcNAc2-type glycan. However, it remains problematic for the complicated synthesis and preparation, and it interacts with glycoprotein folding-related proteins in the ER other than CRT. This suggests that the development of CRT ligands still can be improved. In this study, we developed a hybrid binding concept, which encompasses concurrent binding of ligands to CRT lectin and chaperone domains. We synthesized a CRT-targeting glycan ligand with a glycan and hydrophobic aglycone for CRT lectin and chaperone domain binding, respectively. The thermal shift assay with the CRT-targeting glycan demonstrated that binding was enhanced by simultaneous glycan and hydrophobic aglycone binding. The affinity of the CRT-targeting ligand showed isothermal titration calorimetry approximately 50-fold stronger than that of the glycan alone, thereby supporting the hybrid binding concept. In addition, the CRT-targeting ligand inhibited chaperone function. Overall, these results indicate that the hybrid binding concept may be useful as a novel strategy for the development of CRT ligands and inhibitors.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco you should know.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-25 DOI: 10.1093/glycob/cwaf016

Yukie Akune-Taylor

引用次数: 0

Meeting report of the GlySpace Alliance and GaLSIC symposium. GlySpace 联盟和 GaLSIC 研讨会的会议报告。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-25 DOI: 10.1093/glycob/cwaf019

Kiyoko F Aoki-Kinoshita, Frederique Lisacek, Raja Mazumder, Rene Ranzinger, Michael Tiemeyer, Issaku Yamada, Nicolle H Packer

引用次数: 0

Glyco-Forum.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-25 DOI: 10.1093/glycob/cwaf017

引用次数: 0

Charge matters: how flanking substrate charge modulates O-glycan Core elongation. 电荷问题：侧翼底物电荷如何调节 O-聚糖核心伸长。

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-03-25 DOI: 10.1093/glycob/cwaf014

Collin J Ballard, Matthew R Smutny, Lam D Chau, Collin K Wong, Haley M Aharoni, Hana K Lee, Digantkumar G Chapla, Ramon Hurtado-Guerrero, Kelley W Moremen, Thomas A Gerken

{"title":"Charge matters: how flanking substrate charge modulates O-glycan Core elongation.","authors":"Collin J Ballard, Matthew R Smutny, Lam D Chau, Collin K Wong, Haley M Aharoni, Hana K Lee, Digantkumar G Chapla, Ramon Hurtado-Guerrero, Kelley W Moremen, Thomas A Gerken","doi":"10.1093/glycob/cwaf014","DOIUrl":"10.1093/glycob/cwaf014","url":null,"abstract":"Mucin type O-glycan core elongation is typically performed by the C1GALT1, B3GNT6, and ST6GalNAc-I/-II O-glycosyltransferases. These enzymes target the Tn antigen (GalNAc-O-Thr/Ser) dictating the fate of O-glycan elongation, playing important roles in health and disease. Changes in transferase expression and glycan structure are commonly associated with diseases such as cancer, Tn-syndrome, and ulcerative colitis. Despite their significance, their substrate specificities and their biological roles remain elusive. Here, we examine the roles of flanking glycopeptide substrate charge using a library of differently charged glycopeptides and a small library of PSGL-1 Thr57 based charged glycopeptides. We found that C1GALT1 was most influenced by flanking charge preferring negatively charged substrates, while B3GNT6 and ST6GalNAc-II were less influenced, showing unique N- and C-terminal charge preferences. Interestingly, ST6GalNAc-I was not influenced by flanking charge. These charge specificities were further maintained against the charged PSGL-1 glycopeptides, although ST6GalNAc-I showed an increased preference towards a remote N-terminal positive charge. The observed charge preferences were to a large part driven by substrate interactions with the electrostatic surface of the transferase. We propose that negative flanking charge may assist C1GALT1 in targeting key glycosites such as in PSGL-1 and podoplanin. Our findings are consistent with a Golgi hierarchy, where the cis-Golgi localized GalNAc-Ts and C1GALT1 determine the site and thus fate of glycosylation, while the trans-Golgi less-specific ST6GalNAc-I provides a final capping function. This characterization of substrate charge preference furthers our understanding of how these enzymes select their substrates and may contribute to our understanding of their biological roles.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malectin, an endoplasmic reticulum-resident lectin, promotes malignant behavior of human hepatocellular carcinoma.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-02-28 DOI: 10.1093/glycob/cwaf007

Dong Yu, Fu Ming-Fen, Liu Shao-Min, Yu Hai-Yang, Ge Xiao-Xiao, Zhang Lei, Hu Dan, Qin Sheng-Ying

引用次数: 0

RCL glycosylation of serum corticosteroid-binding globulin: implications in cortisol delivery and septic shock.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-02-28 DOI: 10.1093/glycob/cwaf013

Anastasia Chernykh, Zeynep Sumer-Bayraktar, Jessica H Lee, Emily J Meyer, David J Torpy, Morten Thaysen-Andersen

{"title":"RCL glycosylation of serum corticosteroid-binding globulin: implications in cortisol delivery and septic shock.","authors":"Anastasia Chernykh, Zeynep Sumer-Bayraktar, Jessica H Lee, Emily J Meyer, David J Torpy, Morten Thaysen-Andersen","doi":"10.1093/glycob/cwaf013","DOIUrl":"10.1093/glycob/cwaf013","url":null,"abstract":"Corticosteroid-binding globulin (CBG) is a serum glycoprotein that binds and delivers anti-inflammatory cortisol to inflammatory sites through neutrophil elastase-mediated proteolysis of an exposed reactive centre loop (RCL) on CBG. Timely and tissue-specific delivery of cortisol is critical to alleviate inflammation including in life-threatening septic shock conditions. Herein, we firstly summarise our recently published report of functional RCL O- and N-glycosylation events of serum CBG (Chernykh, J Biol Chem, 2023). A key finding of that published work was the LC-MS/MS-based discovery of RCL O-glycans at Thr342 and Thr345 of serum CBG and their inhibitory roles in neutrophil elastase-mediated RCL proteolysis. While these observations are of significance as they implicate RCL O-glycosylation as a potential regulator of cortisol delivery, the link to septic shock remains unexplored. To this end, we used a similar LC-MS/MS approach to profile the RCL O-glycosylation of CBG purified from serum of twelve septic shock patients. Serum CBG from all patients exhibited RCL O-glycosylation comprising (di)sialyl T (NeuAc1-2Gal1GalNAc1) core 1-type O-glycan structures decorating exclusively the Thr342 site. Importantly, relative to less severe cases, individuals presenting with the most severe illness displayed elevated RCL O-glycosylation upon ICU admission, suggesting a previously unknown link to septic shock severity. Overall, we have elucidated the coordinated RCL N- and O-glycosylation events of serum CBG, which improve our understanding of molecular mechanisms governing the timely and tissue-specific delivery of cortisol to inflammatory sites. This work provides clues to molecular aberrations and disease mechanisms underpinning septic shock.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting lectin microarray data.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-02-28 DOI: 10.1093/glycob/cwaf006

Hiroaki Tateno, Lara K Mahal, Ten Feizi, Carsten Kettner, James C Paulson

{"title":"The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting lectin microarray data.","authors":"Hiroaki Tateno, Lara K Mahal, Ten Feizi, Carsten Kettner, James C Paulson","doi":"10.1093/glycob/cwaf006","DOIUrl":"10.1093/glycob/cwaf006","url":null,"abstract":"The MIRAGE (Minimum Information Required for a Glycomics Experiment) project has been established by experts in glycobiology, glycoanalytics, and glycoinformatics under the auspieces of the Beilstein-Institut. The working group aims to develop guidelines for reporting results from various experiments and analyses conducted in structural and functional studies of glycans in the scientific literature. Previous guidelines have been established for glycomic analytics, including mass spectrometry and glycan microarrays. Lectin microarrays are used worldwide for glycan profiling of various biological samples, but there are often insufficient reports on information about experimental methods such as sample preparation and fluorescence labeling. Here, we propose guidelines specifically designed to improve the standards for reporting data from lectin microarray analyses. For each of the seven areas in the workflow of a lectin microarray experiment, we provide recommendations for the minimum information that should be included when reporting results. When adopted by the scientific community the MIRAGE lectin microarray guidelines are expected to enhance data interpretation, facilitate comparison of data between laboratories and encourage the deposition of lectin microarray data in international databases.","PeriodicalId":12766,"journal":{"name":"Glycobiology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyco-Forum.

IF 3.4 3区生物学

Glycobiology Pub Date : 2025-02-28 DOI: 10.1093/glycob/cwaf009

引用次数: 0