Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Glycobiology Pub Date : 2025-01-16 DOI:10.1093/glycob/cwae097

Jose Carlos Paredes Franco, Maria Lucia Sampaio Güther, Michael A J Ferguson

引用次数: 0

Abstract

For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

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用凤仙花提取物快速酶法合成GDP-L-[3H]聚焦物。

对于涉及糖基转移酶和核苷酸糖转运体的研究，放射性核苷酸糖是关键试剂。其中，GDP-L-[3H]焦点目前在商业上不可用。在这里，我们提出了一种简单的方法来制备GDP-[3H]- l -聚焦物，利用存在于非传染性和易于培养的原生动物——束状棘虫（Crithidia fasciculata）细胞质中的酶机制，并通过固相萃取离子交换色谱法纯化它。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Glycobiology 生物-生化与分子生物学

CiteScore

7.50

自引率

4.70%

发文量

审稿时长

3 months

期刊介绍： Established as the leading journal in the field, Glycobiology provides a unique forum dedicated to research into the biological functions of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides, and on proteins that specifically interact with glycans (including lectins, glycosyltransferases, and glycosidases). Glycobiology is essential reading for researchers in biomedicine, basic science, and the biotechnology industries. By providing a single forum, the journal aims to improve communication between glycobiologists working in different disciplines and to increase the overall visibility of the field.