Genes to Cells最新文献_第4页

Fertilizable Rat Sperm Is Generated in Mice Using Blastocyst Complementation: An Efficient Method for Producing Rats With ES Cell Traits 利用囊胚互补在小鼠体内产生可受精的大鼠精子：一种产生具有胚胎干细胞特征的大鼠的有效方法

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-05-22 DOI: 10.1111/gtc.70024

Rie Natsume, Kosuke Murata, Hiroaki Taketsuru, Runa Hirayama, Tsugumi Iwasaki, Hideaki Yamashiro, Keizo Takao, Ena Nakatsukasa, Manabu Abe, Kenji Sakimura

{"title":"Fertilizable Rat Sperm Is Generated in Mice Using Blastocyst Complementation: An Efficient Method for Producing Rats With ES Cell Traits","authors":"Rie Natsume, Kosuke Murata, Hiroaki Taketsuru, Runa Hirayama, Tsugumi Iwasaki, Hideaki Yamashiro, Keizo Takao, Ena Nakatsukasa, Manabu Abe, Kenji Sakimura","doi":"10.1111/gtc.70024","DOIUrl":"https://doi.org/10.1111/gtc.70024","url":null,"abstract":"<div>\u0000 \u0000 We developed a novel approach for generating rat offspring using rat embryonic stem (ES) cell-derived sperm produced in mice with the blastocyst complementation method. By optimizing culture conditions, we established naïve male rat ES cells from two transgenic rat strains expressing EGFP and Venus fluorescence, respectively. The pluripotency of these cells was confirmed by the formation of germline chimeras. These ES cells were then injected into blastocysts of germ cell-deficient mice, which resulted in chimeric mice with the ability to produce rat-derived sperm. Histological analysis confirmed the presence of seminiferous tubules and spermatozoa, which are morphologically characteristic of rats, in the chimeric testes. To evaluate the fertilization potential of the chimeric mouse sperm, we performed intracytoplasmic sperm injection (ICSI) to rat oocytes and successfully produced viable offspring carrying ES cell-derived traits. This method eliminates concerns regarding host cell contribution, as all sperm in the chimeras originate from rats, enabling the use of nonfluorescent cells. Furthermore, the absence of competition with host cells is expected to enhance sperm production efficiency. By utilizing germ cell-deficient mice as recipients, this approach offers a cost-effective and efficient strategy for generating genetically modified rats, addressing key limitations in rat ES cell-based genetic engineering.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative Splicing of FBLN2 Generates a Prometastatic Extracellular Matrix in Gastrointestinal Cancers by Determining N-Glycosylation of Fibulin 2 FBLN2的选择性剪接通过决定纤维蛋白2的n -糖基化在胃肠道肿瘤中产生前转移细胞外基质

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-05-21 DOI: 10.1111/gtc.70027

Ryo Funayama, Yujue Wang, Masaki Hosogane, Wei-Chen Kao, Shingo Toyama, Masahiro Ohira, Masaki Matsumoto, Takashi Aizawa, Minoru Kobayashi, Hideaki Karasawa, Shinobu Ohnuma, Keiichi I. Nakayama, Michiaki Unno, Keiko Nakayama

{"title":"Alternative Splicing of FBLN2 Generates a Prometastatic Extracellular Matrix in Gastrointestinal Cancers by Determining N-Glycosylation of Fibulin 2","authors":"Ryo Funayama, Yujue Wang, Masaki Hosogane, Wei-Chen Kao, Shingo Toyama, Masahiro Ohira, Masaki Matsumoto, Takashi Aizawa, Minoru Kobayashi, Hideaki Karasawa, Shinobu Ohnuma, Keiichi I. Nakayama, Michiaki Unno, Keiko Nakayama","doi":"10.1111/gtc.70027","DOIUrl":"https://doi.org/10.1111/gtc.70027","url":null,"abstract":"Fibulin 2 (FBLN2) is an extracellular matrix glycoprotein. Exclusion of exon 9 of FBLN2 is one of the most recurrent splicing events across multiple types of cancer, but its functional relevance in cancer has remained unexplored. We here reveal that the exclusion of exon 9 of FBLN2 results in the loss of a single N-glycosylation site that leads to misfolding of the FBLN2 protein as well as to a reduction in both its stability and secretion efficiency. Indeed, the extracellular matrix of human colorectal cancer tissue exhibits a reduced abundance of FBLN2. This deficiency of FBLN2 together with a concomitant increase in the abundance of fibronectin 1 in the tumor microenvironment promotes the adhesion and migration of colorectal cancer cells. Our data thus suggest that the alternative splicing of FBLN2 exon 9 generates a prometastatic extracellular environment in cancer tissue by determining FBLN2 glycosylation.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polished Rice Regulates Maturation but Not Survival of Secondary Cells in Drosophila Male Accessory Gland 精米调节果蝇雄性副腺次生细胞成熟但不影响其存活

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-05-10 DOI: 10.1111/gtc.70025

Shinichi Otsune, Mirai Matsuka, Chisato Shirakashi, Xuanshuo Zhang, Hideki Nakagoshi

引用次数: 0

Profiling of RBM20-Regulated CaMKIIδ Splice Variants Across the Heart, Skeletal Muscle, and Olfactory Bulbs 心脏、骨骼肌和嗅球中rbm20调控的CaMKIIδ剪接变异的分析

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-05-08 DOI: 10.1111/gtc.70021

Yui Maeda, Yuri Yamasu, Hidehito Kuroyanagi

{"title":"Profiling of RBM20-Regulated CaMKIIδ Splice Variants Across the Heart, Skeletal Muscle, and Olfactory Bulbs","authors":"Yui Maeda, Yuri Yamasu, Hidehito Kuroyanagi","doi":"10.1111/gtc.70021","DOIUrl":"https://doi.org/10.1111/gtc.70021","url":null,"abstract":"Calcium/calmodulin-dependent protein kinase IIδ (CaMKIIδ), encoded by the Camk2d gene, plays key regulatory roles in various Ca2+-regulated cellular processes. Extensive alternative splicing of the Camk2d gene generates multiple CaMKIIδ splice variants that exhibit differential roles. Despite significant advances in understanding the functions of CaMKIIδ, the full repertoire of Camk2d splice variants in a variety of tissues and their distinct roles in physiological and pathological contexts remain incompletely characterized due to the complex nature of multiple alternative splicing events. Here, we conducted long-read amplicon sequencing to investigate the murine Camk2d splice variants in the heart, skeletal muscle, and olfactory bulbs and show that mRNAs in the heart and skeletal muscle have shorter 3'UTRs. Our results in this study suggest that a key regulator of Camk2d splicing, RNA-binding motif protein 20 (RBM20), whose gain-of-function mutations cause dilated cardiomyopathy, is crucial for the expression of heart-specific splice variants. Olfactory bulbs specifically express novel splice variants that utilize a mutually exclusive exon 6B and/or an alternative polyadenylation site in a novel exon 17.5 in an RBM20-independent manner. The tissue-specific repertoire of CaMKIIδ splice variants and their aberrant expression in disease model animals will help in understanding their roles in physiological and pathological contexts.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Global Methylation Changes in F1 Mice Sperm DNA Induced by Gestational Arsenic Exposure Are Re-Established in F2 Somatic Cells but Not in F2 Germ Cells 妊娠期砷暴露诱导的F1小鼠精子DNA整体甲基化变化特征在F2体细胞中重新建立，而在F2生殖细胞中没有

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-29 DOI: 10.1111/gtc.70022

Takehiro Suzuki, Kazuyuki Okamura, Keiko Nohara

{"title":"Characteristics of Global Methylation Changes in F1 Mice Sperm DNA Induced by Gestational Arsenic Exposure Are Re-Established in F2 Somatic Cells but Not in F2 Germ Cells","authors":"Takehiro Suzuki, Kazuyuki Okamura, Keiko Nohara","doi":"10.1111/gtc.70022","DOIUrl":"https://doi.org/10.1111/gtc.70022","url":null,"abstract":"<div>\u0000 \u0000 Gestational exposure to chemicals has been reported to transmit epigenetic modifications of germ cells not only to somatic cells but also to the germ cells of the next generation, resulting in adverse effects. Arsenic is one of the environmental chemicals of greatest concern, but it is not precisely clarified whether and how epigenetic modifications of F1 sperm caused by gestational exposure are transmitted to the next generation of somatic cells and germ cells. In the present study, we examined the effects of arsenic exposure during gestation on DNA methylation in germ line and somatic cells of the F2. The DNA methylome of F2 sperm was analyzed by reduced representation bisulfite sequencing (RRBS) and compared to that of F2 liver and testis. We found that F2 liver and testis DNA from the arsenic group exhibited the decrease in global DNA methylation levels and bias of DMC distribution toward hypoDMC observed in F1 sperm DNA which we have previously reported, but F2 sperm DNA did not exhibit those characteristics. These studies suggest that the characteristics of epigenetic modifications in F1 sperm induced by gestational arsenic exposure are reestablished in F2 somatic cells but not in F2 germ cells.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron-Dependent JMJD1A-Mediated Demethylation of H3K9me2 Regulates Gene Expression During Adipogenesis in a Spatial Genome Organization-Dependent Manner 铁依赖性jmjd1a介导的H3K9me2去甲基化以空间基因组组织依赖的方式调节脂肪形成过程中的基因表达

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-28 DOI: 10.1111/gtc.70023

Shinnosuke Masuda, Tetsuro Komatsu, Safiya Atia, Tomohiro Suzuki, Mayuko Hayashi, Atsushi Toyoda, Hiroshi Kimura, Takeshi Inagaki

{"title":"Iron-Dependent JMJD1A-Mediated Demethylation of H3K9me2 Regulates Gene Expression During Adipogenesis in a Spatial Genome Organization-Dependent Manner","authors":"Shinnosuke Masuda, Tetsuro Komatsu, Safiya Atia, Tomohiro Suzuki, Mayuko Hayashi, Atsushi Toyoda, Hiroshi Kimura, Takeshi Inagaki","doi":"10.1111/gtc.70023","DOIUrl":"https://doi.org/10.1111/gtc.70023","url":null,"abstract":"Chromatin restructuring across multiple hierarchical scales directs lineage-specific gene expression during cell differentiation. Here, we investigated the iron-dependent demethylation of histone H3 lysine 9 dimethylation (H3K9me2) by the demethylase jumonji domain-containing 1A (JMJD1A) in adipocyte differentiation. Using the 3T3-L1 adipocyte differentiation model, we show that JMJD1A knockdown increases H3K9me2 levels, whereas forced expression of wild-type JMJD1A reduces H3K9me2 levels within the A compartment, as defined by megabase scale high-throughput chromosome conformation capture (Hi-C) data. In contrast, a JMJD1A mutant defective in iron coordination was unable to demethylate H3K9me2. Genome-wide analyses of H3K9me2 levels at transcription start sites on a kilobase scale demonstrated that JMJD1A targets genes involved in peroxisome proliferator-activated receptor signaling and lipid metabolism in an iron-dependent manner, supporting a model in which H3K9me2 demethylation facilitates adipogenic transcription networks. Furthermore, we examined the relationship between H3K9me2 and lamin B1 levels within lamina-associated domains (LADs) specifically reorganized during differentiation. Although LADs with higher H3K9me2 exhibited modestly elevated lamin B1 association in preadipocytes, lamin B1 levels declined during differentiation regardless of H3K9me2 status. These findings emphasize the importance of the iron-dependent enzymatic function in JMJD1A and broaden our understanding of how specific H3K9 demethylases coordinate compartmentalized epigenetic programs during adipogenesis.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “The Impact of SETBP1 Mutations in Neurological Diseases and Cancer” 更正“SETBP1突变对神经系统疾病和癌症的影响”

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-20 DOI: 10.1111/gtc.70020

引用次数: 0

Cryo-EM Structures of Native Chromatin Units From Human Cells 人类细胞原生染色质单位的冷冻电镜结构

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-14 DOI: 10.1111/gtc.70019

Suguru Hatazawa, Yoshiyuki Fukuda, Yuki Kobayashi, Lumi Negishi, Masahide Kikkawa, Yoshimasa Takizawa, Hitoshi Kurumizaka

{"title":"Cryo-EM Structures of Native Chromatin Units From Human Cells","authors":"Suguru Hatazawa, Yoshiyuki Fukuda, Yuki Kobayashi, Lumi Negishi, Masahide Kikkawa, Yoshimasa Takizawa, Hitoshi Kurumizaka","doi":"10.1111/gtc.70019","DOIUrl":"https://doi.org/10.1111/gtc.70019","url":null,"abstract":"In eukaryotic cells, genomic DNA is compacted by nucleosomes, as basic repeating units, into chromatin. The nucleosome arrangement in chromatin fibers could be an important determinant for chromatin folding, by which genomic DNA is regulated in the nucleus. To study the structures of chromatin units in cells, we have established a method for the structural analysis of native mono- and poly-nucleosomes prepared from HeLa cells. In this method, the chromatin in isolated nuclei was crosslinked to preserve the proximity information between nucleosomes, followed by chromatin fragmentation by micrococcal nuclease treatment. The mono- and poly-nucleosomes were then fractionated by sucrose gradient ultracentrifugation, and their structures were analyzed by cryo-electron microscopy. Cryo-electron microscopy single particle analysis and cryo-electron tomography visualized a native nucleosome structure and secondary nucleosome arrangements in cellular chromatin. This method provides a complementary strategy to fill the gap between in vitro and in situ analyses of chromatin structure.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 12th 3R + 3C International Symposium: A Meeting for Research Into DNA Replication, Repair, and Recombination, as Well as Chromatin, Chromosomes, and the Cell Cycle 第12届3R + 3C国际研讨会：DNA复制、修复和重组，以及染色质、染色体和细胞周期的研究会议

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-10 DOI: 10.1111/gtc.70018

Tsutomu Katayama, Masatoshi Fujita, Tatsuro S. Takahashi

{"title":"The 12th 3R + 3C International Symposium: A Meeting for Research Into DNA Replication, Repair, and Recombination, as Well as Chromatin, Chromosomes, and the Cell Cycle","authors":"Tsutomu Katayama, Masatoshi Fujita, Tatsuro S. Takahashi","doi":"10.1111/gtc.70018","DOIUrl":"https://doi.org/10.1111/gtc.70018","url":null,"abstract":"<div>\u0000 \u0000 The 12th 3R + 3C international symposium focused on cutting-edge research into the molecular mechanisms and regulatory systems of DNA replication, repair, and recombination (3R) as well as those of chromatin dynamics, chromosome architecture, and the cell cycle (3C). It also covered pioneering research into how these processes control cell growth, cell homeostasis, differentiation, development, and aging, in addition to how they contribute to diseases such as cancer, chromosomal abnormalities, and evolution of organisms. In terms of methodology, the symposium highlighted new trends in single-molecule/single-cell analysis, cryo-electron microscopy analysis, kinetic analysis of higher-order protein complexes, informatic analysis of genome dynamics, and new mathematical and theoretical analyses. Held in Fukuoka City center from November 18 to 22, 2024, this symposium attracted about 250 participants, including approximately 150 from Japan and nearly 100 from overseas. To foster mutual understanding and exchange between different fields, all the oral presentations took place in a single conference hall throughout the symposium. This format facilitated active and in-depth discussions among participants, including young researchers, graduate students, and postdoctoral fellows.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INTS15, A Subunit of the Integrator Complex, Plays a Key Regulatory Role in Cell Cycle and Differentiation INTS15是整合子复合体的一个亚基，在细胞周期和分化中起着关键的调节作用

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-04-07 DOI: 10.1111/gtc.70015

Noriyuki Azuma, Yuki Yamaguchi, Taku Tanaka, Emiko Matsuzaka, Yuki Saida, Tadashi Yokoi, Hiroshi Handa, Jun Hirayama, Hiroshi Nishina

{"title":"INTS15, A Subunit of the Integrator Complex, Plays a Key Regulatory Role in Cell Cycle and Differentiation","authors":"Noriyuki Azuma, Yuki Yamaguchi, Taku Tanaka, Emiko Matsuzaka, Yuki Saida, Tadashi Yokoi, Hiroshi Handa, Jun Hirayama, Hiroshi Nishina","doi":"10.1111/gtc.70015","DOIUrl":"https://doi.org/10.1111/gtc.70015","url":null,"abstract":"<div>\u0000 \u0000 We previously reported that Integrator complex subunit 15 (INTS15) is a causative gene for an autosomal-dominant eye disease named variable panocular malformations (VPMs) and that INTS15 stably interacts with the Integrator complex to support snRNA 3′ end processing, thereby controlling mRNA splicing. Here we report another critical function of INTS15 in cell cycle control. HeLa cells and human iPS cells were engineered to overexpress INTS15 expression in a cumate-responsive manner and used to study its role in the regulation of cell cycle and differentiation. INTS15 activates the expression of p53 and p21 to induce G1 arrest when overexpressed. In in vitro differentiation of iPS cells, INTS15 promotes the formation of the three germ layers as well as differentiation into late retinal tissues. Meanwhile, INTS15 knockdown results in defects in G2/M progression and apoptosis. Moreover, INTS15 expression levels vary substantially by cell type and flactuate during the cell cycle. Thus, this study reveals a novel biological aspect of the Integrator complex and demonstrates its potential practical applications.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0