Genes to Cells最新文献_第5页

Cryo-EM Analysis of a Unique Subnucleosome Containing Centromere-Specific Histone Variant CENP-A 含有着丝粒特异性组蛋白变体CENP-A的独特亚核小体的冷冻电镜分析

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-24 DOI: 10.1111/gtc.70016

Osamu Kawasaki, Yoshimasa Takizawa, Iori Kiyokawa, Hitoshi Kurumizaka, Kayo Nozawa

{"title":"Cryo-EM Analysis of a Unique Subnucleosome Containing Centromere-Specific Histone Variant CENP-A","authors":"Osamu Kawasaki, Yoshimasa Takizawa, Iori Kiyokawa, Hitoshi Kurumizaka, Kayo Nozawa","doi":"10.1111/gtc.70016","DOIUrl":"https://doi.org/10.1111/gtc.70016","url":null,"abstract":"In eukaryotes, genomic DNA is stored in the nucleus as nucleosomes, in which a DNA segment is wrapped around a protein octamer consisting of two each of the four histones, H2A, H2B, H3, and H4. The core histones can be replaced by histone variants or altered with covalent modifications, contributing to the regulation of chromosome structure and nuclear activities. The formation of an octameric histone core in nucleosomes is widely accepted. Recently, the H3–H4 octasome, a novel nucleosome-like structure with a histone octamer consisting solely of H3 and H4, has been reported. CENP-A is the centromere-specific histone H3 variant and determines the position of kinetochore assembly during mitosis. CENP-A is a distant H3 variant sharing approximately 50% amino acid sequence with H3. In this study, we found that CENP-A and H4 also formed an octamer without H2A and H2B in vitro. We determined the structure of the CENP-A–H4 octasome at 3.66 Å resolution. In the CENP-A–H4 octasome, an approximately 120-base pair DNA segment was wrapped around the CENP-A–H4 octameric core and displayed the four CENP-A RG-loops, which are the direct binding sites for another centromeric protein, CENP-N.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of the DNA Topoisomerase IIβ C-Terminal Region on the Selective Degradation Induced by ICRF-193 Treatment DNA拓扑异构酶i β c末端区对ICRF-193诱导的选择性降解的影响

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-24 DOI: 10.1111/gtc.70017

Shinji Kawano, Shogo Ikeda

{"title":"The Impact of the DNA Topoisomerase IIβ C-Terminal Region on the Selective Degradation Induced by ICRF-193 Treatment","authors":"Shinji Kawano, Shogo Ikeda","doi":"10.1111/gtc.70017","DOIUrl":"https://doi.org/10.1111/gtc.70017","url":null,"abstract":"<div>\u0000 \u0000 ICRF-193, a catalytic inhibitor of DNA topoisomerase II (TOP2), induces the formation of the TOP2 closed-clamp intermediate. Only the ICRF-193-induced topoisomerase IIβ (TOP2B) closed clamp is known to be selectively and rapidly degraded in vertebrates, but the details are unknown. In this study, we focused on the C-terminal domain (CTD) of TOP2B, which regulates its nuclear dynamics, and sought the region that affects the ICRF-193-induced TOP2B closed-clamp degradation. Using a CTD-swapping mutant between topoisomerase IIα (TOP2A) and TOP2B, we found that the CTD of TOP2B, but not that of TOP2A, is involved in the TOP2B closed-clamp degradation. Furthermore, we identified the C-terminal region (CTR) of TOP2B (amino acids 1570-1621) as a domain that affects TOP2B closed-clamp degradation using a CTR truncation mutant (ΔCTR). A transcription inhibitor inhibited the ICRF-193-induced TOP2B closed-clamp degradation, but the TOP2B ΔCTR closed-clamp degradation was not. In addition, the results of co-immunoprecipitation and immunofluorescence staining showed that the proximity of TOP2B and RNA polymerase II on chromatin in the presence of ICRF-193 tended to be reduced by the lack of TOP2B CTR. Taken together, our data indicate that the TOP2B CTR is involved in the transcription-dependent TOP2B closed-clamp degradation induced by ICRF-193.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “DNA Double-Strand Breaks Induce the Expression of Flavin-Containing Monooxygenase and Reduce Root Meristem Size in Arabidopsis thaliana” 对“DNA双链断裂诱导含黄素单加氧酶的表达和减少拟南芥根分生组织大小”的修正。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-21 DOI: 10.1111/gtc.70014

引用次数: 0

Improvement of Targeting Efficiency by Promoter Replacement of Markers in Integration Vectors 整合载体中启动子替换标记提高靶向效率。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-20 DOI: 10.1111/gtc.70013

Akihisa Matsuyama, Atsushi Hashimoto, Manabu Arioka, Minoru Yoshida

{"title":"Improvement of Targeting Efficiency by Promoter Replacement of Markers in Integration Vectors","authors":"Akihisa Matsuyama, Atsushi Hashimoto, Manabu Arioka, Minoru Yoshida","doi":"10.1111/gtc.70013","DOIUrl":"10.1111/gtc.70013","url":null,"abstract":"<div>\u0000 \u0000 To establish a gene expression system that reflects physiological conditions, we developed a series of vectors that can be integrated into the chromosome. Compared with the integration vectors employing double-crossover recombination, single-crossover integration vectors have the advantage of high transformation efficiency. However, because single-crossover recombination generates repeat sequences upstream and downstream of the integrated fragment, this strategy is often associated with a risk that an integrated fragment may pop out from the chromosome during cultivation. Here, we assessed the frequency of pop-out using a fission yeast single-crossover integration vector, pDUAL. We also examined the effect of shortening the repeats on pop-out by employing a strategy involving heterologous replacement of the promoter for the leu1 marker in the vector. Due to the intrinsic low frequency of pop-out, the effect of promoter conversion on pop-out was negligible, if any. However, a clear ameliorative effect was observed in obtaining the desirable transformants in which a vector fragment was correctly inserted at the targeted locus, a result that may be driven by the limited potential for recombination in the promoter replacement construct.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterochromatin Protein Swi6 Suppresses Aberrant Gene Conversion at mat Loci by Adjusting the Balance Between the Two Pathways of Swi2 and Rad57 异染色质蛋白Swi6通过调节Swi2和Rad57两条通路之间的平衡来抑制mat位点上的异常基因转化

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-13 DOI: 10.1111/gtc.70012

Takumi Fujioka, Yota Murakami, Shinya Takahata

{"title":"Heterochromatin Protein Swi6 Suppresses Aberrant Gene Conversion at mat Loci by Adjusting the Balance Between the Two Pathways of Swi2 and Rad57","authors":"Takumi Fujioka, Yota Murakami, Shinya Takahata","doi":"10.1111/gtc.70012","DOIUrl":"https://doi.org/10.1111/gtc.70012","url":null,"abstract":"<div>\u0000 \u0000 Heterochromatin protein 1 (HP1) is a highly conserved, canonical factor involved in heterochromatin formation. HP1 has been shown to interact with proteins other than silencing factors and heterochromatin effectors. In fission yeast, the loss of the HP1 homolog Swi6 disrupts heterochromatin structure and affects mating type switching at the mat locus, where heterochromatin exists; however, cell growth is unaffected. In this study, we focused on the Swi6 dimerization domain, which provides a binding surface for various interactors. We isolated a distinctive swi6H321Q mutant that does not affect heterochromatin structure but causes variegation in growth defects and abnormal recombination at the mat locus. This mutation disrupts the interaction between Swi6 and Swi2, a mat locus-specific recombination protein. The AT-hook motif of Swi2, which is also required for chromatin localization at the mat locus, is necessary for growth inhibition, suggesting that mislocalization of Swi2 at the mat locus induces growth inhibition. Genetic analysis revealed that abnormal recombination at the mat region was independent of Swi2 but dependent on the Rad57-dependent homologous recombination pathway. These results suggest that Swi6 plays an important role in gene conversion at the mat locus by producing an appropriate selection of homologous recombination factors.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advantages of Mutant Generation by Genome Rearrangements of Non-Conventional Yeast via Direct Nuclease Transfection 直接核酸酶转染非常规酵母基因组重排产生突变体的优势

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-11 DOI: 10.1111/gtc.70010

Arisa H. Oda, Taishi Yasukawa, Miki Tamura, Ayumu Sano, Naohisa Masuo, Kunihiro Ohta

{"title":"Advantages of Mutant Generation by Genome Rearrangements of Non-Conventional Yeast via Direct Nuclease Transfection","authors":"Arisa H. Oda, Taishi Yasukawa, Miki Tamura, Ayumu Sano, Naohisa Masuo, Kunihiro Ohta","doi":"10.1111/gtc.70010","DOIUrl":"https://doi.org/10.1111/gtc.70010","url":null,"abstract":"We previously developed a genome engineering method (TAQing2.0) based on the direct delivery of DNA endonucleases into living cells, which induces genome rearrangements even in non-sporulating nonconventional yeasts without introducing foreign DNA. Using TAQing2.0 and conventional mutagenesis (by nitrosoguanidine), we obtained mutant asexual Candida utilis strains capable of growing under highly acidic conditions (pH 1.8). Whole genome resequencing revealed that the genomic sequences of mutants generated by both methods contain a negligible small population of unmappable sequences, suggesting that both types of mutants can be regarded as equivalent to naturally occurring mutants. TAQing2.0 mutants exhibit multiple genome rearrangements with few point mutations, whereas conventional mutagenesis produces numerous point mutations. This feature enabled us to easily identify candidate genes (e.g., LYP1 homolog) responsible for acid resistance. TAQing2.0 is a powerful and versatile tool for mutant production and gene hunting without invasion of foreign DNA.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Role of Trap Clone Accumulation Areas (TCAAs) in Sustaining Pluripotency in Mouse Embryonic Stem Cells 陷阱克隆积累区（TCAAs）在维持小鼠胚胎干细胞多能性中的潜在作用

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-09 DOI: 10.1111/gtc.70011

Masatake Araki, Luna Ikeda, Takumi Yonemori, Kumiko Yoshinobu, Mariko Yamane, Takumi Ichikawa, Kimi Araki

{"title":"Potential Role of Trap Clone Accumulation Areas (TCAAs) in Sustaining Pluripotency in Mouse Embryonic Stem Cells","authors":"Masatake Araki, Luna Ikeda, Takumi Yonemori, Kumiko Yoshinobu, Mariko Yamane, Takumi Ichikawa, Kimi Araki","doi":"10.1111/gtc.70011","DOIUrl":"https://doi.org/10.1111/gtc.70011","url":null,"abstract":"<div>\u0000 \u0000 Analysis of gene trap clones (TCs) revealed the existence of regions where TCs accumulate in the absence of genes. These regions were designated as trap clone accumulation areas (TCAAs). To ascertain the physiological function of TCAAs, negative control regions devoid of genes and TCs (NC1 and NC11), two randomly selected known gene sets (G1 and G11), and a set of genes presumed to be involved in maintaining pluripotency in embryonic stem (ES) cells (GP) were generated and compared with TCAAs. The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) results indicated that TCAAs exhibited characteristics comparable to G1, G11, and GP, suggesting an open chromatin structure. Oct4-chromatin immunoprecipitation-sequencing (ChIP-seq) demonstrated that TCAAs had markedly elevated signals compared to G1 and 11, and a comparable level to that of GP. With regard to H3K4me1 and H3K27ac, which are associated with enhancer activity, TCAAs were observed to exhibit significantly higher levels than G1 and 11 and a comparable level to that of GP. Furthermore, approximately half of the super-enhancers overlapped with TCAAs in an ES cell-specific manner. These findings suggest that TCAAs are involved in maintaining the pluripotency of mouse ES cells.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge” 更正“蛋白激酶aPKC以及小gtpase RhoA和Cdc42通过招募ROCK激酶到前沿来部分调节中性粒细胞趋化性”

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-03-06 DOI: 10.1111/gtc.70009

{"title":"Correction to “The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge”","authors":"","doi":"10.1111/gtc.70009","DOIUrl":"https://doi.org/10.1111/gtc.70009","url":null,"abstract":"Naito, A., S. Kamakura, J. Hayase, et al. 2025. “The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge.” Genes to Cells 30, no. 2: e70002. https://doi.org/10.1111/gtc.70002.In the “Funding” section on page 1, the text “This work was supported by Japan Society for the Promotion of Science (JP21H02698, JP21H05267, and JP22K06901).” was incorrect. This should have read: “This work was supported by Japan Society for the Promotion of Science (JP21H02698, JP21H05267, and JP22K06901) and by the fellowship from the Takeda Science Foundation.”In the “Acknowledgments” section on page 17, the text “This work was supported in part by JSPS (Japan Society for the Promotion of Science) KAKENHI Grants JP21H02698 (to H.S.), JP21H05267 (to H.S.), and JP22K06901 (to S.K.).” was incorrect. This should have read: “This work was supported in part by JSPS (Japan Society for the Promotion of Science) KAKENHI Grants JP21H02698 (to H.S.), JP21H05267 (to H.S.), and JP22K06901 (to S.K.), and by the fellowship from the Takeda Science Foundation (to A.N.).”We apologize for these errors.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Mucosa-Associated Microbiota in Formalin-Fixed, Paraffin-Embedded Tissues From Southern Thai Patients With Familial Adenomatous Polyposis 来自泰国南部家族性腺瘤性息肉病患者的福尔马林固定、石蜡包埋组织中粘膜相关微生物群的特征

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-02-25 DOI: 10.1111/gtc.70008

Jukrayupat Fongmanee, Worrawit Wanitsuwan, Warapond Wanna, Komwit Surachat, Charinrat Saechan, Kanitta Srinoun, Hansuk Buncherd, Supinya Thanapongpichat, Kanet Kanjanapradit, Natta Tansila

{"title":"Characterization of Mucosa-Associated Microbiota in Formalin-Fixed, Paraffin-Embedded Tissues From Southern Thai Patients With Familial Adenomatous Polyposis","authors":"Jukrayupat Fongmanee, Worrawit Wanitsuwan, Warapond Wanna, Komwit Surachat, Charinrat Saechan, Kanitta Srinoun, Hansuk Buncherd, Supinya Thanapongpichat, Kanet Kanjanapradit, Natta Tansila","doi":"10.1111/gtc.70008","DOIUrl":"https://doi.org/10.1111/gtc.70008","url":null,"abstract":"<div>\u0000 \u0000 Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome associated with germline mutations in the adenomatous polyposis coli (APC) gene. Patients eventually may develop colorectal cancer (CRC) if they are not diagnosed in the early stages. Dysbiosis is an important contributing factor to the complex events in carcinogenesis, which are poorly understood. First, 25 tissue samples from 13 patients with FAP at Songklanagarind Hospital were classified as nontumor (n = 18) or tumor tissues (n = 7). Following isolation, 5 DNA samples of insufficient quantity and quality were excluded. The 16S rRNA gene targeting the V3–V4 region was sequenced, and the sequencing data were analyzed using bioinformatics tools. The abundance of Romboutsia and Clostridium genera and Lachnospiraceae NK4A136 was significantly higher in tumor tissues than that in nonneoplastic samples. Furthermore, several bacterial genera, including Acinetobacter, Paracoccus, Brevundimonas, and Brevibacillus, were predominant or key taxa in nontumor mucosae. We found an alteration in the mucosa-associated microbiota composition of southern Thai patients that may have contributed to the tumorigenesis of FAP. These findings may improve the knowledge of the potential roles of microbes in FAP and aid the development of preventive measures for cancer development and progression through modulation of the gut microbiota.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a Novel Caco-2-Based Cell Culture System for Human Sapovirus Propagation 建立基于 Caco-2 细胞培养的新型人类沙波病毒传播系统

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-02-25 DOI: 10.1111/gtc.70007

Yuya Fukuda, Azusa Ishikawa, Ryoka Ishiyama, Reiko Takai-Todaka, Kei Haga, Yuichi Someya, Tomomi Kimura-Someya, Kazuhiko Katayama

{"title":"Establishment of a Novel Caco-2-Based Cell Culture System for Human Sapovirus Propagation","authors":"Yuya Fukuda, Azusa Ishikawa, Ryoka Ishiyama, Reiko Takai-Todaka, Kei Haga, Yuichi Someya, Tomomi Kimura-Someya, Kazuhiko Katayama","doi":"10.1111/gtc.70007","DOIUrl":"https://doi.org/10.1111/gtc.70007","url":null,"abstract":"Human sapovirus (HuSaV), first identified in the 1970s, is a significant cause of acute gastroenteritis, particularly in young children. Despite its clinical significance, research on HuSaV has been limited due to the absence of a reliable cell culture system. In 2020, a breakthrough study reported that HuSaV GI.1 and GII.3 strains could be cultured and serially propagated using HuTu80 cells in the presence of bile acids. However, in 2024, a subsequent study reported that effective replication in HuTu80 cells requires specialized cells that have undergone over 100 passages. In this study, we sought to identify an alternative cell culture system for HuSaV. HuSaV GI.1 can replicate and be serially propagated using Caco-2 cells under bile acid supplementation. Importantly, the Caco-2 cells were freshly sourced from the American Type Culture Collection, ensuring reproducibility for laboratories worldwide. Furthermore, Caco-2MC cells were established via single-cell cloning from in-house Caco-2/Cas9 cells with 91.5% HuSaV-susceptible. HuSaV strains GI.1, GI.2, GI.3, GII.1, GII.3, and GV.1 were successfully propagated using Caco-2MC cells, with RNA copy numbers increasing up to 4.4 log10-fold within 5 days post-infection. This efficient HuSaV cell culture system represents a significant advancement in HuSaV research.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0