Genes to Cells最新文献_第6页

Transcriptomic Regulation by Astrocytic m6A Methylation in the mPFC 星形细胞m6A甲基化在mPFC中的转录组调控

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-02-04 DOI: 10.1111/gtc.70003

You-Lu Wen, Fang Guo, Ting-ting Gu, Yan-ping Zeng, Xiong Cao

{"title":"Transcriptomic Regulation by Astrocytic m6A Methylation in the mPFC","authors":"You-Lu Wen, Fang Guo, Ting-ting Gu, Yan-ping Zeng, Xiong Cao","doi":"10.1111/gtc.70003","DOIUrl":"https://doi.org/10.1111/gtc.70003","url":null,"abstract":"Astrocytes, the most prevalent type of glial cells, have been found to play a crucial part in numerous physiological functions. By offering metabolic and structural support, astrocytes are vital for the proper functioning of the brain and regulating information processing and synaptic transmission. Astrocytes located in the medial prefrontal cortex (mPFC) are highly responsive to environmental changes and have been associated with the development of brain disorders. One of the primary mechanisms through which the brain responds to environmental factors is epitranscriptome modification. M6-methyladenosine methylation is the most prevalent internal modification of eukaryotic messenger RNA (mRNA), and it significantly impacts transcript processing and protein synthesis. However, the effects of m6A on astrocyte transcription and function are still not well understood. Our research demonstrates that ALKBH5, an RNA demethylase of m6A found in astrocytes, affects gene expression in the mPFC. These findings suggest that further investigation into the potential role of astrocyte-mediated m6A methylation in the mPFC is warranted.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 2","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gtc.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WDR74-Mediated Ribosome Biogenesis and Proteome Dynamics During Mouse Preimplantation Development 小鼠着床前发育过程中wdr74介导的核糖体生物发生和蛋白质组动力学。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-22 DOI: 10.1111/gtc.70001

Ayaka Kakihara, Marino Maemura, Atsushi Hatano, Masaki Matsumoto, Yu-ichi Tsukada

{"title":"WDR74-Mediated Ribosome Biogenesis and Proteome Dynamics During Mouse Preimplantation Development","authors":"Ayaka Kakihara, Marino Maemura, Atsushi Hatano, Masaki Matsumoto, Yu-ichi Tsukada","doi":"10.1111/gtc.70001","DOIUrl":"10.1111/gtc.70001","url":null,"abstract":"<div>\u0000 \u0000 Preimplantation embryonic development is orchestrated by dynamic changes in the proteome and transcriptome, regulated by mechanisms such as maternal-to-zygotic transition. Here, we employed label-free quantitative proteomics to comprehensively analyze proteome dynamics from germinal vesicle oocytes to blastocysts in mouse embryos. We identified 3490 proteins, including 715 consistently detected across all stages, revealing stage-specific changes in proteins associated with translation, protein modification, and mitochondrial metabolism. Comparison with transcriptomic data highlighted a low correlation between mRNA and protein levels, underscoring the significance of non-transcriptional regulatory mechanisms during early development. Additionally, we analyzed WD repeat-containing protein 74 (WDR74)-deficient embryos generated using CRISPR-Cas9 genome editing. WDR74, a pre-60S ribosome maturation factor, was found to be critical for ribosome biogenesis and cell division. Furthermore, WDR74 deficiency led to a significant reduction in ribosomal protein large subunit and impaired progression beyond the morula stage. Key ribosomal proteins such as ribosomal protein L24 (RPL24) and ribosomal protein L26 (RPL26), which influence cell division timing, were notably affected, while small subunit proteins remained largely unchanged. Taken together, our study demonstrates the utility of integrating genome editing with proteomic analysis to elucidate molecular mechanisms underlying early embryogenesis, and provides new insights into protein-level regulation of preimplantation development.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of Evolutionarily Young Endogenous Retroviruses Is Implicated in COVID-19 Immunopathology 进化年轻内源性逆转录病毒的激活与COVID-19免疫病理有关

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-19 DOI: 10.1111/gtc.13194

Reia Yoshida, Hitoshi Ohtani

{"title":"Activation of Evolutionarily Young Endogenous Retroviruses Is Implicated in COVID-19 Immunopathology","authors":"Reia Yoshida, Hitoshi Ohtani","doi":"10.1111/gtc.13194","DOIUrl":"10.1111/gtc.13194","url":null,"abstract":"The dysfunction of the innate immune system is well-described as a clinical characteristic of COVID-19. While several groups have reported human endogenous retroviruses (ERVs) as enhancing factors of immune reactivity, characterization of the COVID-19-specific ERVs has not yet been sufficiently conducted. Here, we revealed the transcriptome profile of more than 500 ERV subfamilies and innate immune response genes in eight different cohorts of platelet, peripheral blood mononuclear cells (PBMCs), lung, frontal cortex of brain, ventral midbrain, pooled human umbilical vein endothelial cells (pHUVECs), placenta, and cardiac microvascular endothelial cells (HCMEC) from COVID-19 patients (total; n = 124) and normal samples (total; n = 53) using publicly available datasets. While upregulation of ERV subfamilies was found in platelets, PBMCs, and placenta, the immune reactivity was confined to only platelets and PBMCs. It is noteworthy that the evolutionary ages of the upregulated ERV subfamilies detected in platelets and PBMCs were younger than other ERV subfamilies, but the tendency was not seen in the upregulated ERV subfamilies in placenta. The results suggest that only evolutionarily young ERVs can function as enhancing factors of the immune reactivity in COVID-19 patients. The finding should be instrumental in understanding the COVID-19 immunopathology.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“Chance and Necessity” on the Molecular Evolution of REV3 (a Catalytic Subunit of DNA Polymerase ζ)—The Dual Roles of Translesion and Neuronal Extension REV3 （DNA聚合酶ζ的催化亚基）分子进化的“偶然与必然”——翻译和神经元延伸的双重作用。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-17 DOI: 10.1111/gtc.13189

Kagemasa Kajiwara, Kouichi Yamada

{"title":"“Chance and Necessity” on the Molecular Evolution of REV3 (a Catalytic Subunit of DNA Polymerase ζ)—The Dual Roles of Translesion and Neuronal Extension","authors":"Kagemasa Kajiwara, Kouichi Yamada","doi":"10.1111/gtc.13189","DOIUrl":"10.1111/gtc.13189","url":null,"abstract":"<div>\u0000 \u0000 Catalytic subunit of DNA polymerase ζ (REV3), involved in translesion-replication is evolutionarily conserved from yeast and plants to higher eukaryotes. However, a large intermediate domain is inserted in REV3 of humans and mice. The domain has “DUF4683” region, which is significantly similar to human neurite extension and migration factor (NEXMIF). This region was also found in REV3 of invertebrates such as Ciona intestinalis (sea squirt) … and Lingula anatina (Brachiopoda). We hypothesize foreign sequences were introduced into the Rev3 genes in the ancestral species of L. anatina, which would have gradually evolved into the DUF4683 region through overly complicated processes. Besides DUF4683, various exogenous sequences would have been inserted during the REV3 evolution. Therefore, insertion events of foreign sequences are all products of “necessity”. tBLASTn analysis of the Callorhinchus milii (elephant shark) genome with the C. milii REV3 sequence identified three neural factors (NEXMIF, NEXMIF-like and AHDC1) in distinct positions of the genome. These factors may have differentiated from the Rev3 gene in Chondrichthyes, which had experienced two rounds of whole-genome duplication, and may have evolved into neurite-forming proteins in vertebrates. L. anatina has the DUF4683 C-terminal proximal consensus (SPPRA/CWSP) in REV3. However, the consensus was not necessarily maintained in Mollusca, the closely related animal phylum of L. anatina. Just as written by Jacques Monod, we assume “chance” (mutation in DNA) and “necessity” (selection at the “organism” population level) were frequently repeated on the Rev3 in Cambrian ancestors. As a result, certain species developed the DUF4683 consensus of a neurite extension activity.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIF1α Plays a Crucial Role in the Development of TFE3–Rearranged Renal Cell Carcinoma by Orchestrating a Metabolic Shift Toward Fatty Acid Synthesis HIF1α通过协调向脂肪酸合成的代谢转变在tfe3重排肾细胞癌的发展中起关键作用。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-14 DOI: 10.1111/gtc.13195

Hidekazu Nishizawa, Shintaro Funasaki, Wenjuan Ma, Yoshiaki Kubota, Kazuhide Watanabe, Yuichiro Arima, Shoichiro Kuroda, Takaaki Ito, Mitsuko Furuya, Takanobu Motoshima, Akira Nishiyama, Sally Mehanna, Yorifumi Satou, Hisashi Hasumi, Ryosuke Jikuya, Kazuhide Makiyama, Tomohiko Tamura, Yuichi Oike, Yasuhito Tanaka, Toshio Suda, Laura S. Schmidt, W. Marston Linehan, Masaya Baba, Tomomi Kamba

{"title":"HIF1α Plays a Crucial Role in the Development of TFE3–Rearranged Renal Cell Carcinoma by Orchestrating a Metabolic Shift Toward Fatty Acid Synthesis","authors":"Hidekazu Nishizawa, Shintaro Funasaki, Wenjuan Ma, Yoshiaki Kubota, Kazuhide Watanabe, Yuichiro Arima, Shoichiro Kuroda, Takaaki Ito, Mitsuko Furuya, Takanobu Motoshima, Akira Nishiyama, Sally Mehanna, Yorifumi Satou, Hisashi Hasumi, Ryosuke Jikuya, Kazuhide Makiyama, Tomohiko Tamura, Yuichi Oike, Yasuhito Tanaka, Toshio Suda, Laura S. Schmidt, W. Marston Linehan, Masaya Baba, Tomomi Kamba","doi":"10.1111/gtc.13195","DOIUrl":"10.1111/gtc.13195","url":null,"abstract":"Tumor development often requires cellular adaptation to a unique, high metabolic state; however, the molecular mechanisms that drive such metabolic changes in TFE3–rearranged renal cell carcinoma (TFE3-RCC) remain poorly understood. TFE3-RCC, a rare subtype of RCC, is defined by the formation of chimeric proteins involving the transcription factor TFE3. In this study, we analyzed cell lines and genetically engineered mice, demonstrating that the expression of the chimeric protein PRCC-TFE3 induced a hypoxia-related signature by transcriptionally upregulating HIF1α and HIF2α. The upregulation of HIF1α by PRCC-TFE3 led to increased cellular ATP production by enhancing glycolysis, which also supplied substrates for the TCA cycle while maintaining mitochondrial oxidative phosphorylation. We crossed TFE3-RCC mouse models with Hif1α and/or Hif2α knockout mice and found that Hif1α, rather than Hif2α, is essential for tumor development in vivo. RNA-seq and metabolomic analyses of the kidney tissues from these mice revealed that ketone body production is inversely correlated with tumor development, whereas de novo lipid synthesis is upregulated through the HIF1α/SREBP1-dependent mechanism in TFE3-RCC. Our data suggest that the coordinated metabolic shift via the PRCC-TFE3/HIF1α/SREBP1 axis is a key mechanism by which PRCC-TFE3 enhances cancer cell metabolism, promoting tumor development in TFE3-RCC.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell RNA-Sequencing of Zebrafish Olfactory Epithelium Identifies Odor-Responsive Candidate Olfactory Receptors 斑马鱼嗅觉上皮单细胞rna测序鉴定气味反应候选嗅觉受体。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-09 DOI: 10.1111/gtc.13191

Misaki Takaoka, Towako Hiraki-Kajiyama, Nobuhiko Miyasaka, Takahiro Hino, Kenji Kondo, Yoshihiro Yoshihara

{"title":"Single-Cell RNA-Sequencing of Zebrafish Olfactory Epithelium Identifies Odor-Responsive Candidate Olfactory Receptors","authors":"Misaki Takaoka, Towako Hiraki-Kajiyama, Nobuhiko Miyasaka, Takahiro Hino, Kenji Kondo, Yoshihiro Yoshihara","doi":"10.1111/gtc.13191","DOIUrl":"10.1111/gtc.13191","url":null,"abstract":"Single-cell RNA-sequencing (scRNA-seq) is a powerful method to comprehensively overlook gene expression profiles of individual cells in various tissues, providing fundamental datasets for classification of cell types and further functional analyses. Here we adopted scRNA-seq analysis for the zebrafish olfactory sensory neurons which respond to water-borne odorants and pheromones to elicit various behaviors crucial for survival and species preservation. Firstly, a single-cell dissociation procedure of the zebrafish olfactory rosettes was optimized by using cold-active protease, minimizing artifactual neuronal activation. Secondly, various cell types were classified into distinct clusters, based on the expressions of well-defined marker genes. Notably, we validated non-overlapping expressions of different families of olfactory receptors among the clusters of olfactory sensory neurons. Lastly, we succeeded in estimating candidate olfactory receptors responding to a particular odor stimulus by carefully scrutinizing correlated expressions of immediate early genes. Thus, scRNA-seq is a useful measure for the analysis of olfactory sensory neurons not only in classifying functional cell types but also in identifying olfactory receptor genes for given odorants and pheromones.","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SRPKs Homolog Dsk1 Regulates Homologous Recombination Repair in Schizosaccharomyces pombe SRPKs同源Dsk1调控裂糖酵母同源重组修复。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-09 DOI: 10.1111/gtc.13192

Guangchun Lu, Zhiheng Tang, Mei Wu, Li Liu, Mitchell Opoku, Kaicheng Bian, Rui Ruan, Jinjie Shang, Jia Liu, Gang Feng

{"title":"SRPKs Homolog Dsk1 Regulates Homologous Recombination Repair in Schizosaccharomyces pombe","authors":"Guangchun Lu, Zhiheng Tang, Mei Wu, Li Liu, Mitchell Opoku, Kaicheng Bian, Rui Ruan, Jinjie Shang, Jia Liu, Gang Feng","doi":"10.1111/gtc.13192","DOIUrl":"10.1111/gtc.13192","url":null,"abstract":"<div>\u0000 \u0000 Serine-arginine protein kinases (SRPKs) play important roles in diverse biological processes such as alternative splicing and cell cycle. However, the functions of SRPKs in DNA damage response remain unclear. Here we characterized the function of SRPKs homolog Dsk1 in regulating DNA repair in the fission yeast Schizosaccharomyces pombe. We demonstrated that Dsk1 defective mutants of loss of the gene, spacer domain, and kinase activity as well as its overexpression mutant exhibited sensitivities of replication stress. Genetic analysis revealed that the loss of dsk1+ compromised the efficiency of homologous recombination (HR) repair, and Dsk1 was probably involved in the Rad52- and Rad51-dependent HR repair pathways. Interestingly, Dsk1 translocated into the nucleus upon replication stress and directly interacted with Rad51-mediator Rad52 and phosphorylated Rad52-Ser365 residue. The Rad52-Ser365 phosphorylation-defective mutant was slightly sensitive to replication stress, and the phosphorylation-mimicking mutants exhibited more sensitivities, which were partially correlated with phenotypes of the loss- and gain-of-function of dsk1+. This study uncovers a potential HR repair regulator Dsk1 in response to replication stress and implies that its homolog SRPKs may have the conserved targets and functions in higher eukaryotes.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swimming Into Future Breakthroughs From Kyoto, Japan: Report of the 18th International Zebrafish Conference (IZFC2024) 从日本京都游向未来的突破：第18届国际斑马鱼会议（IZFC2024）报告。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-07 DOI: 10.1111/gtc.13193

Hiromi Hirata, Tohru Ishitani, Hitoshi Okamoto

{"title":"Swimming Into Future Breakthroughs From Kyoto, Japan: Report of the 18th International Zebrafish Conference (IZFC2024)","authors":"Hiromi Hirata, Tohru Ishitani, Hitoshi Okamoto","doi":"10.1111/gtc.13193","DOIUrl":"10.1111/gtc.13193","url":null,"abstract":"<div>\u0000 \u0000 The 18th International Zebrafish Conference (IZFC2024) took place from August 17 to 21, 2024, at Miyako Messe in Kyoto, Japan. This conference attracted 641 researchers from around the world along with 83 virtual participants, making it the largest gathering since the COVID-19 pandemic. The event featured two keynote lectures, three award lectures, 36 plenary talks, 90 oral presentations, and 374 poster presentations. Participants also engaged in luncheons, workshops, and community sessions, all aimed at exploring innovative technologies, enhancing scientific environments and strengthening connections within the research community. Additionally, the conference included cultural elements that allowed participants to immerse themselves in Japanese traditions, enriching their overall experience. This commitment to blending scientific inquiry with cultural appreciation ensured that participants gained valuable insights into the latest research while enjoying a taste of Japan's rich heritage. Overall, IZFC2024 successfully promoted collaboration and innovation in zebrafish research while celebrating the unique cultural aspects of Japan, making this conference an unforgettable event for everyone involved.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Transition From Interindividual Uniformity to Diversity in Appearance and Transcriptional Features at Midlife in Caenorhabditis elegans 中年秀丽隐杆线虫外貌和转录特征从个体间一致性到多样性的过渡。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2025-01-01 DOI: 10.1111/gtc.13187

Masaharu Uno, Masanori Nono, Chika Takahashi, Saya Kishimoto, Emiko Okabe, Takuya Yamamoto, Eisuke Nishida

{"title":"A Transition From Interindividual Uniformity to Diversity in Appearance and Transcriptional Features at Midlife in Caenorhabditis elegans","authors":"Masaharu Uno, Masanori Nono, Chika Takahashi, Saya Kishimoto, Emiko Okabe, Takuya Yamamoto, Eisuke Nishida","doi":"10.1111/gtc.13187","DOIUrl":"10.1111/gtc.13187","url":null,"abstract":"<div>\u0000 \u0000 During embryogenesis, organisms function as a robust system that ensures uniformity within individuals, but they lose robustness and develop variations at advanced ages. However, when and how organisms lose this robustness remains largely elusive. Here, we identified a sharp transition from interindividual uniformity to diversity in the appearance and transcriptional features of age-matched Caenorhabditis elegans in midlife. Convolutional neural network analysis of individual appearance alterations revealed that the transition occurs in midlife, which coincides with the cessation of egg-laying activity and increased motility defects. This period represents the transition from the young state, marked by shared homogeneous features among same-age individuals, to the old state, marked by shared among old individuals. Transcriptional coherence within the age-matched individuals shows essentially the same transition, coinciding with the appearance features. These findings provide a new framework for understanding the aging trajectory in C. elegans, demonstrating the occurrence of the loss of robust control over appearance and transcriptional homeostasis in midlife.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulforaphane Induces Transient Reactive Oxygen Species-Mediated DNA Damage in HeLa Cells 萝卜硫素诱导HeLa细胞瞬时活性氧介导的DNA损伤。

IF 1.3 4区生物学

Genes to Cells Pub Date : 2024-12-27 DOI: 10.1111/gtc.13190

Sakine Kobayashi, Seiya Nishiba, Chinatsu Sato, Kazuya Toriumi, Yuduki Someya, Noritaka Adachi, Shigeki Takeda, Aya Kurosawa

{"title":"Sulforaphane Induces Transient Reactive Oxygen Species-Mediated DNA Damage in HeLa Cells","authors":"Sakine Kobayashi, Seiya Nishiba, Chinatsu Sato, Kazuya Toriumi, Yuduki Someya, Noritaka Adachi, Shigeki Takeda, Aya Kurosawa","doi":"10.1111/gtc.13190","DOIUrl":"10.1111/gtc.13190","url":null,"abstract":"<div>\u0000 \u0000 Sulforaphane (SFN), an isothiocyanate found in plants of the Brassicaceae family, possesses antioxidant, apoptosis-inducing, and radiosensitizing effects. As one of the mechanisms of cytotoxicity by SFN, SFN has been suggested to be involved in the induction of DNA damage and inhibition of DNA repair. Recently, we reported on the potency of SFN in inducing single-ended double-strand breaks (DSBs) that are caused by the collision of replication forks with single-strand breaks (SSBs). However, the mechanism of SSB accumulation by SFN remains unclear. In this study, we examined the effect of SFN on SSB-inducing factors in HeLa cells. Although the inhibitory effect of SFN on DNA topoisomerase I was not observed, we found that the reduced form of glutathione (GSH; an antioxidant) level was decreased in an SFN concentration-dependent manner. Furthermore, the addition of ascorbic acid partially increased the viability of SFN-treated HeLa cells. We subsequently observed that poly(ADP-ribose) accumulated in SFN-treated HeLa cells, which occurs during early SSB repair. Collectively, these findings suggest that SFN may transiently induce SSBs via reactive oxygen species in HeLa cells.\u0000 </div>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0