Genes and Environment最新文献

{"title":"Telomere length in peripheral leukocytes is a sensitive marker for assessing genetic damage among workers exposed to isopropanol, lead and noise: the case of an electronics manufacturer.","authors":"Yao Lu, Xinxia Liu, Zhiqiang Zhao, Xiaoyan Ou, Yarui Yang, Qing Wei, Jingli Chen, Jun Jiang, Yi Sun, Heping Zhao, Sai Wu, Yun He","doi":"10.1186/s41021-021-00226-x","DOIUrl":"https://doi.org/10.1186/s41021-021-00226-x","url":null,"abstract":"<p><strong>Background: </strong>Workers in electronics manufacturers may be exposed to various occupational hazards such as isopropanol, lead, and noise. Telomeres are special segments of cap-like DNA protein complex at end of liner chromosomes in eukaryotic cells. Telomere length is a potential marker of genetic damage. The aim of this study is to evaluate the effect of occupational hazards on the relative telomere length (rTL) of peripheral blood cells of workers in an electronics manufacturer, and to explore whether relative telomere length could be a biomarker for assessing genetic damage in the electronics manufacturing industry.</p><p><strong>Methods: </strong>We investigated a large-scale electronics manufacturer in the Pearl River Delta Region. We ultimately collected 699 qualified workers (248 with isopropanol exposure, 182 with lead exposure, 157 with noise exposure, and 112 controls). During physical examination of the workers, we gave them questionnaires to understand their health statuses and living habits. We also collected peripheral blood samples from these workers to test exposure levels and rTL in the leucocytes.</p><p><strong>Results: </strong>The concentrations of air isopropanol in all monitored workshops was 25.3 mg/m<sup>3</sup> and air lead smoke was 0.020 mg/m<sup>3</sup>. The maximum equivalent continuous A sound level noise exposure position was 82.2dB (A). All were lower than those in the Occupational Exposure Limits in Workplaces in China. Urinary acetone in the isopropanol exposed group was 1.04 (0, 1.50) mg/L, and cumulative urinary acetone was 1.48 (0, 5.09) mg-years/L. Blood lead levels (BLLs) were 28.57 (22.77, 37.06) µg/dL, and cumulative blood lead levels (CBLLs) were 92.75 (55.47, 165.13) µg-years/dL. rTL was different between occupational exposed workers and controls: rTL was 0.140 units (95 % CI: 0.022, 0.259) shorter in lead exposed workers and 0.467 units (95 % CI: 0.276-0.658) shorter in noise exposed workers compared to the controls. There is no statistical difference in rTL between isopropanol exposure workers and the controls. In order to elucidate the relationship between rTL and occupational hazards exposure, we divided the isopropanol exposure workers into three groups (0, ~1.43 mg/L, and >1.43 mg/L). None of the rTL difference was statistically significant among exposed workers at different uroacetone levels (P>0.05). The groups with ≥100 µg/dL blood lead had shorter rTL than the group with blood lead below 100 µg/dL (F=4.422, P=0.013). We incorporated age, gender, birthplace, race, education level, smoking, and alcohol consumption into the linear regression equation. Only blood lead concentration (X) was entered into the regression equation, yielding a multivariate linear regression equation of Y=0.397-0.124X (F=8.091, P=0.005). Workers with different hearing loss also had statistically significant differences in rTL (F=5.731, P=0.004). rTL was a protective factor for the occurrence of noise-induc","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"57"},"PeriodicalIF":1.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39820732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary selenium intake on gut microbiota in older population in Enshi region.","authors":"Zi-Xiong Zhang,&nbsp;Hua Xiang,&nbsp;Guo-Gen Sun,&nbsp;Yan-Hua Yang,&nbsp;Chen Chen,&nbsp;Tuo Li","doi":"10.1186/s41021-021-00220-3","DOIUrl":"https://doi.org/10.1186/s41021-021-00220-3","url":null,"abstract":"<p><strong>Background: </strong>The microbial ecosystem in the human gut varies between individuals with differences in diet. Selenium is one of most common trace elements in everyday diet, and selenium intake affects the human gut microbiota. We studied the effect of selenium intake on the gut microbiota in regions of Enshi with different distributions of selenium.</p><p><strong>Methods: </strong>One hundred elderly subjects (>65 years) were recruited from high-selenium and low-selenium areas in Enshi and blood, nail, and fecal specimens were obtained. The selenium contents in these samples were determined in triplicate by hydride generation atomic fluorescence spectrometry. DNA was extracted from fecal specimens and the microbial diversity was analyzed by 16 S RNA.</p><p><strong>Results: </strong>The selenium contents in the blood and nails were significantly different between the high- and low-selenium areas, and the composition of the intestinal microbiota, including abundance and extent of intestinal flora, was altered. The function and metabolic pathways of the gut microbiota showed clear differences.</p><p><strong>Conclusions: </strong>As a trace element in human diet, selenium intake is an important factor that affects the intestinal microbiota and is likely involved in many human diseases. This study provides new clues and ideas for studying the correlation between selenium and human health.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"56"},"PeriodicalIF":1.7,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39834649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetics in neurodegenerative disorders induced by pesticides.","authors":"Guangxia Yu,&nbsp;Qianqian Su,&nbsp;Yao Chen,&nbsp;Lingyan Wu,&nbsp;Siying Wu,&nbsp;Huangyuan Li","doi":"10.1186/s41021-021-00224-z","DOIUrl":"https://doi.org/10.1186/s41021-021-00224-z","url":null,"abstract":"<p><p>Neurodegenerative diseases are becoming major socio-economic burdens. However, most of them still have no effective treatment. Growing evidence indicates excess exposure to pesticides are involved in the development of various forms of neurodegenerative and neurological diseases through trigger epigenetic changes and inducing disruption of the epigenome. This review summaries studies on epigenetics alterations in nervous systems in relation to different kinds of pesticides, highlighting potential mechanism in the etiology, precision prevention and target therapy of various neurodegenerative diseases. In addition, the current gaps in research and future areas for study were also discussed.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"55"},"PeriodicalIF":1.7,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39714125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PIG-A gene mutation as a genotoxicity biomaker in polycyclic aromatic hydrocarbon-exposed barbecue workers.","authors":"Yiyi Cao,&nbsp;Jing Xi,&nbsp;Chuanxi Tang,&nbsp;Ziying Yang,&nbsp;Weiying Liu,&nbsp;Xinyue You,&nbsp;Nannan Feng,&nbsp;Xin Yu Zhang,&nbsp;Jingui Wu,&nbsp;Yingxin Yu,&nbsp;Yang Luan","doi":"10.1186/s41021-021-00230-1","DOIUrl":"https://doi.org/10.1186/s41021-021-00230-1","url":null,"abstract":"<p><strong>Background: </strong>The PIG-A gene mutation assay is a valuable tool for measuring in vivo gene mutations in blood cells. The human PIG-A assay, used as a potential genotoxicity biomarker, is minimally invasive, sensitive, and cost-efficient; however, the relationship between carcinogen exposure and PIG-A mutations is not well understood.</p><p><strong>Methods: </strong>We investigated the genotoxic effect of red blood cells using PIG-A assay and lymphocyte cytokinesis-block micronucleus test in barbecue restaurant workers (N = 70) exposed to polycyclic aromatic hydrocarbons (PAHs) and self-identified healthy control subjects (N = 56). Urinary PAH metabolites were measured to evaluate internal exposure levels.</p><p><strong>Results: </strong>Multivariate Poisson regression showed that the PAH-exposed workers exhibited significantly higher PIG-A mutant frequency (MF) (8.04 ± 6.81 × 10^- 6) than did the controls (5.56 ± 5.26 × 10^- 6) (RR = 0.707, 95% CI: 0.615-0.812, P < 0.001). These results indicate that PAH exposure is a risk factor for elevated PIG-A MF. The frequencies of micronuclei (MN) and nuclear buds (NBUD) in the PAH-exposed workers (MN: 3.06 ± 2.07 ‰, NBUD: 1.38 ± 1.02 ‰) were also significantly higher than in the controls (MN: 1.46 ± 0.64 ‰, P < 0.001; NBUD: 0.70 ± 0.60 ‰, P < 0.001). Additionally, PIG-A MFs showed better associations with several urinary hydroxylated PAH metabolites (P_2-OH-Flu = 0.032, r_2-OH-Flu = 0. 268; P_2-OH-Phe = 0.022, r_2-OH-Phe = 0.286; P_3-OH-Phe = 0.0312, r_3-OH-Phe = 0.270; P_4-OH-Phe = 0.018, r_4-OH-Phe = 0.296), while the increase in MN, NPB, and NBUD frequencies was not associated with any OH-PAH metabolites; and high-PAH-exposed workers showed the highest PIG-A MFs. Furthermore, there was a significant association between PIG-A MF and PAH exposure levels (Chi-square test for trend, P = 0.006).</p><p><strong>Conclusions: </strong>Our results indicate that an increase in PIG-A MF in barbecue workers could reflect the response to PAH exposure, providing evidence of its potential as a genotoxicity biomarker in human risk assessment.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"54"},"PeriodicalIF":1.7,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8656086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39957076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycoprotein 2 in health and disease: lifting the veil.","authors":"Yingsong Lin,&nbsp;Masahiro Nakatochi,&nbsp;Naoki Sasahira,&nbsp;Makoto Ueno,&nbsp;Naoto Egawa,&nbsp;Yasushi Adachi,&nbsp;Shogo Kikuchi","doi":"10.1186/s41021-021-00229-8","DOIUrl":"https://doi.org/10.1186/s41021-021-00229-8","url":null,"abstract":"<p><p>In 2020, we discovered glycoprotein 2 (GP2) variants associated with pancreatic cancer susceptibility in a genome-wide association study involving the Japanese population. Individuals carrying a missense coding variant (rs78193826) in the GP2 gene resulting in a p.V432M substitution had an approximately 1.5-fold higher risk of developing pancreatic cancer than those without this variant. GP2 is expressed on the inner surface of zymogen granules in pancreatic acinar cells, which are responsible for the sorting, storage and secretion of digestive enzymes. Upon neuronal, hormonal, or other stimulation, GP2 is cleaved from the membrane of zymogen granules and then secreted into the pancreatic duct and intestinal lumen. While the functions of GP2 remain poorly understood, emerging evidence suggests that it plays an antibacterial role in the gastrointestinal tract after being secreted from pancreatic acinar cells. Impaired GP2 functions may facilitate the adhesion of bacteria to the intestinal mucosa. In this review article, we summarize the role of GP2 in health and disease, emphasizing its functions in the gastrointestinal tract, as well as genetic variations in the GP2 gene and their associations with disease susceptibility. We hope that its robust genetic associations with pancreatic cancer, coupled with its emerging role in gastrointestinal mucosal immunity, will spur renewed research interest in GP2, which has been understudied over the past 30 years compared with its paralog uromodulin (UMOD).</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"53"},"PeriodicalIF":1.7,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39690203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNA adductome analysis revealed a reduction in the global level of C5-hydroxymethyl-2'-deoxycytidine in the non-tumoral upper urinary tract mucosa of urothelial carcinoma patients.","authors":"Yuto Matsushita,&nbsp;Yuji Iwashita,&nbsp;Shunsuke Ohtsuka,&nbsp;Ippei Ohnishi,&nbsp;Takashi Yamashita,&nbsp;Hideaki Miyake,&nbsp;Haruhiko Sugimura","doi":"10.1186/s41021-021-00228-9","DOIUrl":"https://doi.org/10.1186/s41021-021-00228-9","url":null,"abstract":"<p><strong>Background: </strong>DNA adducts, covalent modifications to DNA due to exposure to specific carcinogens, cause the mispairing of DNA bases, which ultimately results in DNA mutations. DNA methylation in the promoter region, another type of DNA base modification, alters the DNA transcription process, and has been implicated in carcinogenesis in humans due to the down-regulation of tumor suppressor genes. Difficulties are associated with demonstrating the existence of DNA adducts or chemically modified bases in the human urological system. Apart from aristolochic acid-DNA adducts, which cause urothelial carcinoma and endemic nephropathy in a particular geographical area (Balkan), limited information is currently available on DNA adduct profiles in renal cell carcinoma and upper urinary tract urothelial carcinoma, including renal pelvic cancer and ureteral cancer.</p><p><strong>Method: </strong>To elucidate the significance of DNA adducts in carcinogenesis in the urothelial system, we investigated 53 DNA adducts in the non-tumoral renal parenchyma and non-tumoral renal pelvis of patients with renal cell carcinoma, upper urinary tract urothelial carcinoma, and other diseases using liquid chromatography coupled with tandem mass spectrometry. A comparative analysis of tissue types, the status of malignancy, and clinical characteristics, including lifestyle factors, was performed.</p><p><strong>Results: </strong>C5-Methyl-2'-deoxycytidine, C5-hydroxymethyl-2'-deoxycytidine (5hmdC), C5-formyl-2'-deoxycytidine, 2'-deoxyinosine, C8-oxo-2'-deoxyadenosine, and C8-oxo-2'-deoxyguanosine (8-OHdG) were detected in the renal parenchyma and renal pelvis. 8-OHdG was more frequently detected in the renal pelvis than in the renal cortex and medulla (p = 0.048 and p = 0.038, respectively). 5hmdC levels were significantly lower in the renal pelvis of urothelial carcinoma patients (n = 10) than in the urothelium of patients without urothelial carcinoma (n = 15) (p = 0.010). Regarding 5hmdC levels in the renal cortex and medulla, Spearman's rank correlation test revealed a negative correlation between age and 5hmdC levels (r = - 0.46, p = 0.018 and r = - 0.45, p = 0.042, respectively).</p><p><strong>Conclusions: </strong>The present results revealed a reduction of 5hmdC levels in the non-tumoral urinary tract mucosa of patients with upper urinary tract urothelial carcinoma. Therefore, the urothelial cell epithelia of patients with upper urinary tract cancer, even in non-cancerous areas, may be predisposed to urothelial cancer.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"52"},"PeriodicalIF":1.7,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39795718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thorium and fetal neural tube defects: an epidemiological evidence from large case-control study.","authors":"Bin Wang,&nbsp;Yiming Pang,&nbsp;Yali Zhang,&nbsp;Le Zhang,&nbsp;Rongwei Ye,&nbsp;Lailai Yan,&nbsp;Zhiwen Li,&nbsp;Aiguo Ren","doi":"10.1186/s41021-021-00227-w","DOIUrl":"https://doi.org/10.1186/s41021-021-00227-w","url":null,"abstract":"<p><strong>Background: </strong>Thorium is ubiquitous in the environment and its relationship with birth defects is still under discussion. This study aimed to investigate the associations of maternal exposure to thorium with risk of neural tube defects (NTDs) by using a case-control study, as well as the relationship between thorium exposure and the indoor air pollution from coal combustion.</p><p><strong>Methods: </strong>This study was conducted in 11 local healthcare hospitals during 2003-2007 in Shanxi and Hebei provinces, China. A total of 774 mothers were included as participants who delivering 263 fetuses with NTDs including 123 with anencephaly, 115 with spina bifida, 18 with encephalocele, and 7 other NTD subtypes (cases), and 511 health fetuses without NTDs (controls). Their hair samples were collected as close as to the occipital posterior scalp, of which those grew from 3 months before to 3 months after conception was cut to measure the thorium concentration by inductively coupled plasma-mass spectrometry.</p><p><strong>Results: </strong>We found a higher hair thorium concentration in the total NTD cases with 0.901 (0.588-1.382) ng/g hair [median (inter-quartile range)] than that in the controls with a value of 0.621 (0.334-1.058) ng/g hair. Similar results were found for the three concerned NTD subtypes. Maternal hair thorium concentration above its median of the controls was associated with an increased risk of the total NTDs with an adjusted odds ratio of 1.80 [95% confidence interval (CI), 1.23-2.63)] by adjusting for all confounders. There was obvious dose-response relationship between maternal hair thorium concentration and the risk of total NTDs, as well as their two subtypes (i.e. anencephaly and spina bifida). Maternal hair thorium concentration was positive associated with their exposure level to indoor air pollution from coal combustion during cooking.</p><p><strong>Conclusions: </strong>Overall, our findings revealed that maternal periconceptional thorium exposure was associated with the risk of NTDs in North China. Reducing the coal usage in the household cooking activities may decrease maternal thorium exposure level.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"51"},"PeriodicalIF":1.7,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39771589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimutagenic, anti-inflammatory, and antioxidative activities of the juice of Vitis ficifolia var. Ganebu, a woody vine in the grape family, known as Ryukyu-ganebu in Japan.","authors":"Sakae Arimoto-Kobayashi,&nbsp;Ryoko Hida,&nbsp;Nana Fujii,&nbsp;Ryosuke Mochioka","doi":"10.1186/s41021-021-00225-y","DOIUrl":"https://doi.org/10.1186/s41021-021-00225-y","url":null,"abstract":"<p><strong>Background: </strong>Mutation, inflammation, and oxidative damage including lipid-peroxidation are factors involved in the development of cancer. We investigated the antimutagenic, in vivo and in vitro anti-inflammatory, and antioxidative effects of the juice of Vitis ficifolia var. ganebu (known as Ryukyu-ganebu in Japan) harvested in Kuchinoshima island (hereafter, the juice is referred to as ganebu-K) in comparison with the juice of Vitis coignetiae (crimson glory vine, known as yamabudo in Japan; hereafter, the juice is referred to as yamabudo) which we found antimutagenic and anti-inflammatory effects.</p><p><strong>Results: </strong>Ganebu-K inhibited the mutagenic activity of several carcinogens, MeIQx, IQ, Trp-P-2(NHOH), and MNNG, model compounds of tumor initiation. Using S. typhimurium YG7108, a strain lacking O⁶-methylguanine DNA methyltransferases, ganebu-K showed no significant inhibition of the mutagenicity of MNNG. Thus, DNA repair of O⁶-methylguanine produced by MNNG might be an antimutagenic target of the components in ganebu-K. Topical application of ganebu-K to the dorsal sides of mice resulted in potent suppression of acute edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Ganebu-K, but not yamabudo, exhibited significant inhibition of the induction of prostaglandin E₂ (PGE2) induced by TPA. Components contained in ganebu-K, but not in yamabudo, might be responsible for the inhibition of the induction of PGE2. Ganebu-K inhibited in vivo lipid peroxidation and decreased the level of glutamic oxaloacetic transaminase induced by CCL₄ treatment.</p><p><strong>Conclusions: </strong>These results suggest that the active components in ganebu-K juice are not the same as those in yamabudo, and the components in ganebu-K are attractive candidates as chemopreventive agents.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"50"},"PeriodicalIF":1.7,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39708153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of differences of N⁶-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis.","authors":"Xuejie Qi,&nbsp;Xiao Geng,&nbsp;Juan Zhang,&nbsp;Binpeng Qu,&nbsp;Xin Zhang,&nbsp;Qiang Jia,&nbsp;Wenhui Yin,&nbsp;Cunxiang Bo,&nbsp;Yan Liu,&nbsp;Hao Li,&nbsp;Linlin Sai,&nbsp;Mingming Han,&nbsp;Cheng Peng","doi":"10.1186/s41021-021-00223-0","DOIUrl":"https://doi.org/10.1186/s41021-021-00223-0","url":null,"abstract":"<p><strong>Background: </strong>Increasing evidence suggested N⁶-methyladenosine (m⁶A) modification is crucial for male germline development. However, m⁶A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m⁶A is a highly conserved modification across the species.</p><p><strong>Results: </strong>The results of m⁶A sequences showed that m⁶A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m⁶A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m⁶A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m⁶A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m⁶A-methylated mRNAs.</p><p><strong>Conclusions: </strong>These findings suggested that differentially m⁶A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m⁶A modifications in amphibian species.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"49"},"PeriodicalIF":1.7,"publicationDate":"2021-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39595445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between ADIPOQ rs2241766 SNP and breast cancer risk: a systematic review and a meta-analysis.","authors":"Xue Hu,&nbsp;Chunguo Cui,&nbsp;Tong Sun,&nbsp;Wan Wang","doi":"10.1186/s41021-021-00221-2","DOIUrl":"https://doi.org/10.1186/s41021-021-00221-2","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to conduct a meta-analysis to accurately evaluate the potential association between ADIPOQ rs2241766 gene SNP and breast cancer risk.</p><p><strong>Methods: </strong>A systematic literature search on Cochrane Library, PubMed, Embase, Web of Science and China National Knowledge Infrastructure (CNKI) identified 8 articles with 1692 cases and 1890 controls. Strength of association was evaluated by pooled odds ratio (OR), 95 % confidence interval (CI) and p value. Funnel plots and Begger's regression test were applied for testing the publication bias. Statistical analysis of all data was performed by Stata 12.0.</p><p><strong>Results: </strong>The meta-analysis results indicated that the ADIPOQ rs2241766 gene polymorphism did not significantly associated with the risk of breast cancer for these genetic models (TT vs. TG + GG: OR = 1.20, 95 % CI = 0.77-1.89, p=0.417; TT + TG vs. GG: OR = 1.05, 95 % CI = 0.71-1.56, p=0.805; T vs. G: OR =1.17, 95 % CI = 0.79-1.74, p=0.437).</p><p><strong>Conclusions: </strong>This study indicated that no significant relationship between the ADIPOQ rs2241766 SNP and breast cancer. Further large-scale and well-designed studies will be indispensable to confirm our result.</p>","PeriodicalId":12709,"journal":{"name":"Genes and Environment","volume":" ","pages":"48"},"PeriodicalIF":1.7,"publicationDate":"2021-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39595440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}